Abstract:
:The addition of primary amines to the growth medium of the unicellular green alga Chlamydomonas reinhardtii disrupts cell wall assembly in both vegetative and zygotic cells. Primary amines are competitive inhibitors of the protein-cross-linking activity of transglutaminases. Two independent assays for transglutaminase confirmed a burst of extracellular activity during the early stages of cell wall formation in both vegetative cells and zygotes. When non-inhibiting levels of a radioactive primary amine ((14)C-putrescine) were added to the growth medium, both cell types were labeled in a reaction catalyzed by extracellular transglutaminase. The radioactive label was found specifically in the cell wall proteins of both cell types, and acid hydrolysis of the labeled material released unmodified (14)C-putrescine. Western blots of the proteins secreted at the times of maximal transglutaminase activity in both cell types revealed a single highly cross-reactive 72-kD band when screened with antibodies to guinea pig tissue transglutaminase. Furthermore, the proteins immunoprecipitated by this antiserum in vivo exhibited transglutaminase activity. We propose that this transglutaminase is responsible for an early cell wall protein cross-linking event that temporally precedes the oxidative cross-linking mediated by extracellular peroxidases.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Waffenschmidt S,Kusch T,Woessner JPdoi
10.1104/pp.121.3.1003subject
Has Abstractpub_date
1999-11-01 00:00:00pages
1003-15issue
3eissn
0032-0889issn
1532-2548journal_volume
121pub_type
杂志文章相关文献
PLANT PHYSIOLOGY文献大全abstract::The role of polygalacturonase in leaf abscission was studied in explants of Phaseolus vulgaris L. cv. Red Kidney. Bean polygalacturonase was partially characterized and comparisons were made between the bean enzyme and previously reported higher plant polygalacturonases. Polygalacturonase isolated from bean leaf absci...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.63.6.1133
更新日期:1979-06-01 00:00:00
abstract::Brassinosteroids (BRs) play important roles throughout plant development. Although many genes have been identified that are involved in BR biosynthesis, genetic approaches in Arabidopsis have led to the identification of only one gene, BRI1, that encodes a membrane receptor for BRs. To expand our knowledge of the mole...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.127.1.14
更新日期:2001-09-01 00:00:00
abstract::The light and CO(2) response of (a) photosynthesis, (b) the activation state and total catalytic efficiency (k(cat)) of ribulose-1,5-bisphosphate carboxylase (rubisco), and (c) the pool sizes of ribulose 1,5-bisphosphate, (RuBP), ATP, and ADP were studied in the C(3) annuals Chenopodium album and Phaseolus vulgaris at...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.94.4.1735
更新日期:1990-12-01 00:00:00
abstract::Isolated maize (Zea mays var. kelvedon glory) etioplasts have been used to investigate the relationships between the spectral shifts and ultrastructural changes which occur during light-induced chloroplast development. After primary photoconversion, the Shibata shift was observed as a change from 680 to 670 nm in the ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.55.2.393
更新日期:1975-02-01 00:00:00
abstract::A circadian rhythm of O(2) evolution has been found in Euglena gracilis, Klebs strain Z. The rhythm persists for at least 5 days in constant dim light and temperature, but damps out in constant bright light. The phase of this rhythm can be shifted by a pulse of bright light and the period length is not changed over a ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.61.2.150
更新日期:1978-02-01 00:00:00
abstract::Barley (c.v. Himalaya) aleurone layers were incubated in [(3)H]gibberellin A(1) (GA(1)) at low temperatures. At 3 and 4 C, (3)H-activity was steadily accumulated in aleurone layers, and this accumulation was correlated with significant [(3)H]GA(1) metabolism. At 1 and 1.5 C, metabolism could not be detected, and at th...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.66.5.956
更新日期:1980-11-01 00:00:00
abstract::The occurrence of proteins able to oxidize polyphenols even in the absence of H2O2 was recently reported in mung bean (Vigna radiata L.) hypocotyl cell wall extracts (R. Goldberg, A. Chabanet, A.M. Catesson [1993] In K.G. Welinder, S.K. Rasmussen, C. Penel, H. Greppin, eds, Plant Peroxidases: Biochemistry and Physiolo...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.106.3.1095
更新日期:1994-11-01 00:00:00
abstract::Megasporogenesis is a key step during ovule development in angiosperms, but the small number and inaccessibility of these cells have hampered molecular and genome-wide studies. Thus, many questions remain regarding the molecular basis of cell specification, differentiation, and development in the female gametophyte. H...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.19.01254
更新日期:2020-04-01 00:00:00
abstract::A novel, three-dimensional recording, vibrating probe was used for measuring the density and direction of the endogenous ionic current of cress roots (Lepidium sativum L.) bathed in low salt media (artificial pond water, APW). Roots submerged in regular APW and growing vertically show the following current pattern. Cu...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.100.1.16
更新日期:1992-09-01 00:00:00
abstract::Suspension-cultured cells of sycamore (Acer pseudoplatanus L.) which were immersed in liquid nitrogen after prefreezing to the temperatures from -30 to -50 C in the presence of dimethylsulfoxide and glucose as cryoprotective additive could proliferate vigorously when rewarmed rapidly in water at 40 C. For maintaining ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.54.5.722
更新日期:1974-11-01 00:00:00
abstract::The structure of phosphatidylinositol in barley (Hordeum vulgare) aleurone layers was investigated by chemical degradation. In vivo myo-[2-(3)H]inositol-labeled phosphatidylinositol was first converted to glycerophosphoinositol and, subsequently, after removal of the glycerol moiety, to inositol monophosphate. Here, w...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.98.4.1498
更新日期:1992-04-01 00:00:00
abstract::Roots of taro (Colocasia esculenta [L.] Schott cvs Bun-long and Lehua maoli) exuded increasing concentrations of oxalate with increasing Al stress. This exudation was a specific response to excess Al and not to P deficiency. Addition of oxalate to Al-containing solutions ameliorated the toxic effect of Al. ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.118.3.861
更新日期:1998-11-01 00:00:00
abstract::Ribulose-1,5-bisphosphate carboxylase/oxygenase (EC 4.1.1.39) was crystallized from a heterozygous tobacco (Nicotiana tabacum L.) aurea mutant (Su/su), its wild-type sibling (su/su), and green revertant plants regenerated from green spots found on leaves of haploid Su plants. No differences were found in the specific ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.65.5.828
更新日期:1980-05-01 00:00:00
abstract::In comparison with other cell organelles, the Dunaliella salina plasma membrane was found to be highly enriched in phospholipase C activity toward exogenous [(3)H]phosphatidylinositol 4,5-bisphosphate (PIP(2)). Based on release of [(3)H]inositol phosphates, the plasma membrane exhibited a PIP(2)-phospholipase C activi...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.90.3.1115
更新日期:1989-07-01 00:00:00
abstract::Potato (Solanum tuberosum) tuber, a swollen underground stem, is used as a model system for the study of dormancy release and sprouting. Natural dormancy release, at room temperature, is initiated by tuber apical bud meristem (TAB-meristem) sprouting characterized by apical dominance (AD). Dormancy is shortened by tre...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.112.194076
更新日期:2012-04-01 00:00:00
abstract::Biochemical changes related to cyanogenesis (hydrogen cyanide production) were monitored during maturation of black cherry (Prunus serotina Ehrh.) fruits. At weekly intervals from flowering until maturity, fruits (or selected parts thereof) were analyzed for (a) fresh and dry weights, (b) prunasin and amygdalin levels...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.98.4.1423
更新日期:1992-04-01 00:00:00
abstract::The kinetics of (14)CO(2) carboxylation and decarboxylation in corn root tips were determined to ascertain the sequence of product formation and subsequent utilization, and to obtain further evidence to predict the enzymes mediating the carboxylation and decarboxylations. The carboxylation data indicated that the firs...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.42.5.712
更新日期:1967-05-01 00:00:00
abstract::In maize (Zea mays) and other grasses, changes in orientation of stems are perceived by pulvinal tissue, which responds to the stimulus by differential growth resulting in upward bending of the stem. The amyloplast-containing bundle sheath cells are the sites of gravity perception, although the initial steps of gravit...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.127.1.119
更新日期:2001-09-01 00:00:00
abstract::A new hydroxycinnamoyl-CoA:putrescine hydroxycinnamoyltransferase (PHT) was detected in two variant lines of Nicotiana tabacum L. (TX1, TX4) accumulating markedly different levels of caffeoylputrescine. The enzyme accepted only the aliphatic diamines putrescine, cadaverine and 1,3-diaminopropane at a ratio of 100:33:8...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.89.2.488
更新日期:1989-02-01 00:00:00
abstract::The effect of NaCl and Na(2)SO(4) salinity on NO(3) (-) assimilation in young barley (Hordeum vulgare L. var Numar) seedlings was studied. The induction of the NO(3) (-) transporter was affected very little; the major effect of the salts was on its activity. Both Cl(-) and SO(4) (2-) salts severely inhibited uptake of...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.76.2.321
更新日期:1984-10-01 00:00:00
abstract::Transgenic Nicotiana tabacum (cv Petit Havana SR1) containing high levels of mitochondrial alternative oxidase (AOX) protein due to the introduction of a sense transgene(s) of Aox1, the nuclear gene encoding AOX, were used to investigate mechanisms regulating AOX activity. After purification of leaf mitochondria, a la...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.109.2.353
更新日期:1995-10-01 00:00:00
abstract::Rhythmic fluctuation in the basipetal movement of auxin occurs in corn (Zea mays) coleoptiles oriented either in the vertical or in the horizontal position. This periodicity of transport rate varies from region to region in a horizontal coleoptile. Between an upper and lower half coleoptile (with respect to gravity), ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.52.2.166
更新日期:1973-08-01 00:00:00
abstract::The binding of ferredoxin-NADP reductase to spinach chloroplast membranes was studied by washing the membranes with different media. Release of the enzyme from the thylakoids was greater in 0.75 millimolar EDTA but was not complete inasmuch as 20% the activity remained membrane-bound after three washes.A Scatchard plo...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.69.1.210
更新日期:1982-01-01 00:00:00
abstract::We examined the consistency of the native diploid Nicotiana attenuata (Na)'s herbivore-induced transcriptional changes in the two allotetraploid natives, Nicotiana clevelandii (Nc) and Nicotiana quadrivalvis (Nq), which are thought to be derived from hybridizations with an ancestral Na. An analysis of nuclear-encoded ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.103.037036
更新日期:2004-05-01 00:00:00
abstract::Acyl carrier protein (ACP) is a small (9 kD) acidic protein that is an essential cofactor in plant fatty acid biosynthesis. Most plants have several isoforms of ACP, some of which are expressed constitutively and others that appear to be more tissue specific. Although the critical role of ACP in fatty acid biosynthesi...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.127.1.222
更新日期:2001-09-01 00:00:00
abstract::Recent structure-function analysis of heterologously expressed K+-selective inward-rectifying channels (KIRCs) from plants has revealed that external protons can have opposite effects on different members of the same gene family. An important question is how the diverse response of KIRCs to apoplastic pH is reflected ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.120.1.331
更新日期:1999-05-01 00:00:00
abstract::The relationship between the rate of orthophosphate (Pi) transport into the stroma and the rate of CO(2) fixation by intact chloroplasts was investigated. High Pi concentrations in the medium lead to a depletion of stromal metabolites, due to excessive Pi transport into the stroma, resulting in the inhibition of CO(2)...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.65.4.574
更新日期:1980-04-01 00:00:00
abstract::Since NO(3) (-) availability in the rooting medium seriously limits symbiotic N(2) fixation by soybean (Glycine max [L.] Merr.), studies were initiated to select nodulation mutants which were more tolerant to NO(3) (-) and were adapted to the Midwest area of the United States. Three independent mutants were selected i...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.89.1.169
更新日期:1989-01-01 00:00:00
abstract::Complementary DNA (cDNA) clones encoding calmodulin isoforms were isolated from an Arabidopsis leaf lambdagt10 library by screening with cloned barley calmodulin cDNA probes. Two cDNAs, one a 626-base pair partial-length clone (ACaM-1) and one a 1400-base pair full-length clone (ACaM-2), encode calmodulin polypeptides...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.96.4.1196
更新日期:1991-08-01 00:00:00
abstract::Bundle sheath strands and mesophyll cell extracts have been isolated from maize (Zea mays L.) leaves using a mechanical disruption-filtration technique. Northern blot analysis showed that phosphoenolpyruvate carboxylase (PEPCase; EC 4.1.1.31) mRNA accumulates only in mesophyll cells. The mechanisms regulating the cell...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.82.2.613
更新日期:1986-10-01 00:00:00