Alternative Oxidase Activity in Tobacco Leaf Mitochondria (Dependence on Tricarboxylic Acid Cycle-Mediated Redox Regulation and Pyruvate Activation).

Abstract:

:Transgenic Nicotiana tabacum (cv Petit Havana SR1) containing high levels of mitochondrial alternative oxidase (AOX) protein due to the introduction of a sense transgene(s) of Aox1, the nuclear gene encoding AOX, were used to investigate mechanisms regulating AOX activity. After purification of leaf mitochondria, a large proportion of the AOX protein was present as the oxidized (covalently associated and less active) dimer. High AOX activity in these mitochondria was dependent on both reduction of the protein by DTT (to the noncovalently associated and more active dimer) and its subsequent activation by certain [alpha]-keto acids, particularly pyruvate. Reduction of AOX to its more active form could also be mediated by intramitochondrial reducing power generated by the oxidation of certain tricarboxylic acid cycle substrates, most notably isocitrate and malate. Our evidence suggests that NADPH may be specifically required for AOX reduction. All of the above regulatory mechanisms applied to AOX in wild-type mitochondria as well. Transgenic leaves lacking AOX due to the introduction of an Aox1 antisense transgene or multiple sense transgenes were used to investigate the potential physiological significance of the AOX-regulatory mechanisms. Under conditions in which respiratory carbon metabolism is restricted by the capacity of mitochondrial electron transport, feed-forward activation of AOX by mitochondrial reducing power and pyruvate may act to prevent redirection of carbon metabolism, such as to fermentative pathways.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Vanlerberghe GC,Day DA,Wiskich JT,Vanlerberghe AE,McIntosh L

doi

10.1104/pp.109.2.353

subject

Has Abstract

pub_date

1995-10-01 00:00:00

pages

353-361

issue

2

eissn

0032-0889

issn

1532-2548

pii

109/2/353

journal_volume

109

pub_type

杂志文章
  • Functional analysis of the cellulose synthase genes CesA1, CesA2, and CesA3 in Arabidopsis.

    abstract::Polysaccharide analyses of mutants link several of the glycosyltransferases encoded by the 10 CesA genes of Arabidopsis to cellulose synthesis. Features of those mutant phenotypes point to particular genes depositing cellulose predominantly in either primary or secondary walls. We used transformation with antisense co...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.010931

    authors: Burn JE,Hocart CH,Birch RJ,Cork AC,Williamson RE

    更新日期:2002-06-01 00:00:00

  • Partial purification and characterization of the mitochondrial and peroxisomal isozymes of enoyl-coenzyme a hydratase from germinating pea seedlings.

    abstract::Distinct organellar forms of the beta-oxidation enzyme enoyl-coenzyme A (CoA) hydratase were partially purified and characterized from 2-day germinated pea (Pisum sativum L.) seedlings. The purification was accomplished by disruption of purified mitochondria or peroxisomes, (NH(4))(2)SO(4) fractionation, and gel perme...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.95.2.564

    authors: Miernyk JA,Thomas DR,Wood C

    更新日期:1991-02-01 00:00:00

  • Calcium and Nodulation in Subterranean Clover (Trifolium subterraneum L.).

    abstract::From a study of the effects of Ca ions on the nodulation of subterranean clover in flowing culture solutions it is concluded that root infection or nodule initiation has a higher Ca requirement than either nodule development or host plant growth in the presence of fixed nitrogen.Increasing Ca concentration from 246 to...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.43.9.1362

    authors: Lowther WL,Loneragan JF

    更新日期:1968-09-01 00:00:00

  • Identification and gene expression of anaerobically induced enolase in Echinochloa phyllopogon and Echinochloa crus-pavonis.

    abstract::Enolase (2-phospho-D-glycerate hydrolase, EC 4.2.1.11) has been identified as an anaerobic stress protein in Echinochloa oryzoides based on the homology of its internal amino acid sequence with those of enolases from other organisms, by immunological reactivity, and induction of catalytic activity during anaerobic str...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.109.2.433

    authors: Fox TC,Mujer CV,Andrews DL,Williams AS,Cobb BG,Kennedy RA,Rumpho ME

    更新日期:1995-10-01 00:00:00

  • Characterization of Sucrolysis via the Uridine Diphosphate and Pyrophosphate-Dependent Sucrose Synthase Pathway.

    abstract::The breakdown of sucrose to feed both hexoses into glycolytic carbon flow can occur by the sucrose synthase pathway. This uridine diphosphate (UDP) and pyrophosphate (PPi)-dependent pathway was biochemically characterized using soluble extracts from several plants. The sucrolysis process required the simultaneous pres...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.90.2.635

    authors: Xu DP,Sung SJ,Loboda T,Kormanik PP,Black CC

    更新日期:1989-06-01 00:00:00

  • The DEAD-box RNA Helicase RH50 Is a 23S-4.5S rRNA Maturation Factor that Functionally Overlaps with the Plastid Signaling Factor GUN1.

    abstract::DEAD-box RNA helicases (DBRHs) modulate RNA secondary structure, allowing RNA molecules to adopt the conformations required for interaction with their target proteins. RH50 is a chloroplast-located DBRH that colocalizes and is coexpressed with GUN1, a central factor in chloroplast-to-nucleus signaling. When combined w...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.17.01545

    authors: Paieri F,Tadini L,Manavski N,Kleine T,Ferrari R,Morandini P,Pesaresi P,Meurer J,Leister D

    更新日期:2018-01-01 00:00:00

  • Sodium and potassium absorption by bean stem tissue.

    abstract::The effect of various periods of pretreatment in CaSO(4) solutions (aging) on the absorption of Na and K by bean stem slices was investigated. Freshly sliced tissue absorbed Na over the entire range of concentrations studied (0.02-50 mm). Potassium absorption by fresh tissue was nil at concentrations below 0.5 mm but ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.44.4.547

    authors: Rains DW

    更新日期:1969-04-01 00:00:00

  • Red clover HCT2, a hydroxycinnamoyl-coenzyme A:malate hydroxycinnamoyl transferase, plays a crucial role in biosynthesis of phaselic acid and other hydroxycinnamoyl-malate esters in vivo.

    abstract::In red clover (Trifolium pratense) leaves, phaselic acid (2-O-caffeoyl-L-malate) accumulates to several mmol kg(-1) fresh weight and is a crucial component of a natural system that prevents protein breakdown during harvest and storage of this forage crop. Previously, we identified HCT2, a red clover gene encoding a hy...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.110.166793

    authors: Sullivan ML,Zarnowski R

    更新日期:2011-03-01 00:00:00

  • Wall relaxation and the driving forces for cell expansive growth.

    abstract::When water uptake by growing cells is prevented, the turgor pressure and the tensile stress in the cell wall are reduced by continued wall loosening. This process, termed in vivo stress relaxation, provides a new way to study the dynamics of wall loosening and to measure the wall yield threshold and the physiological...

    journal_title:Plant physiology

    pub_type: 杂志文章,评审

    doi:10.1104/pp.84.3.561

    authors: Cosgrove DJ

    更新日期:1987-01-01 00:00:00

  • Immunocytolocalization of polygalacturonase in ripening tomato fruit.

    abstract::Using tissue blotting and immunocytolocalization, we have investigated the appearance and accumulation of polygalacturonase (PG) during tomato (Lycopersicon esculentum Mill.) fruit ripening. Results show that PG first appears in the collumella region followed by sequential appearance in the exopericarp and endopericar...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.90.1.17

    authors: Tieman DM,Handa AK

    更新日期:1989-05-01 00:00:00

  • Phloem mobility of magnesium.

    abstract::Magnesium-28 was applied to specific leaves of bean (Phaseolus vulgaris) and barley (Hordeum vulgare) plants. After 24 hours, as much as 7% of the absorbed Mg was exported from the treated bean leaves and 11% was transported basipetally from the treated zone of the barley leaves. Transport of Mg did not occur past a h...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.46.1.50

    authors: Steucek GL,Koontz HV

    更新日期:1970-07-01 00:00:00

  • Cytochrome c is released in a reactive oxygen species-dependent manner and is degraded via caspase-like proteases in tobacco Bright-Yellow 2 cells en route to heat shock-induced cell death.

    abstract::To gain some insight into the mechanism of plant programmed cell death, certain features of cytochrome c (cyt c) release were investigated in heat-shocked tobacco (Nicotiana tabacum) Bright-Yellow 2 cells in the 2- to 6-h time range. We found that 2 h after heat shock, cyt c is released from intact mitochondria into t...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.106.078683

    authors: Vacca RA,Valenti D,Bobba A,Merafina RS,Passarella S,Marra E

    更新日期:2006-05-01 00:00:00

  • Arabidopsis NATA1 Acetylates Putrescine and Decreases Defense-Related Hydrogen Peroxide Accumulation.

    abstract::Biosynthesis of the polyamines putrescine, spermidine, and spermine is induced in response to pathogen infection of plants. Putrescine, which is produced from Arg, serves as a metabolic precursor for longer polyamines, including spermidine and spermine. Polyamine acetylation, which has important regulatory functions i...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.16.00446

    authors: Lou YR,Bor M,Yan J,Preuss AS,Jander G

    更新日期:2016-06-01 00:00:00

  • Investigation of respiratory and ion transport properties of aging bean stem slices.

    abstract::Ion and oxygen uptake were studied on aging bean stem slices. Oxygen uptake was high immediately after slicing, decreased to a minimum at 100 minutes, and then increased again. Ion uptake per unit of O(2) uptake data suggested that metabolic energy was utilized almost exclusively for sodium transport in fresh tissue b...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.47.5.663

    authors: Floyd RA,Rains DW

    更新日期:1971-05-01 00:00:00

  • Expression of Escherichia coli glycogen synthase in the tubers of transgenic potatoes (Solanum tuberosum) results in a highly branched starch.

    abstract::A chimeric gene containing the patatin promoter and the transit-peptide region of the small-subunit carboxylase gene was utilized to direct expression of Escherichia coli glycogen synthase (glgA) to potato (Solanum tuberosum) tuber amyloplasts. Expression of the glgA gene product in tuber amyloplasts was between 0.007...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.104.4.1159

    authors: Shewmaker CK,Boyer CD,Wiesenborn DP,Thompson DB,Boersig MR,Oakes JV,Stalker DM

    更新日期:1994-04-01 00:00:00

  • Starch-branching enzyme I-deficient mutation specifically affects the structure and properties of starch in rice endosperm.

    abstract::We have isolated a starch mutant that was deficient in starch-branching enzyme I (BEI) from the endosperm mutant stocks of rice (Oryza sativa) induced by the treatment of fertilized egg cells with N-methyl-N-nitrosourea. The deficiency of BEI in this mutant was controlled by a single recessive gene, tentatively design...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.103.021527

    authors: Satoh H,Nishi A,Yamashita K,Takemoto Y,Tanaka Y,Hosaka Y,Sakurai A,Fujita N,Nakamura Y

    更新日期:2003-11-01 00:00:00

  • The SCABRA3 nuclear gene encodes the plastid RpoTp RNA polymerase, which is required for chloroplast biogenesis and mesophyll cell proliferation in Arabidopsis.

    abstract::In many plant species, a subset of the genes of the chloroplast genome is transcribed by RpoTp, a nuclear-encoded plastid-targeted RNA polymerase. Here, we describe the positional cloning of the SCABRA3 (SCA3) gene, which was found to encode RpoTp in Arabidopsis (Arabidopsis thaliana). We studied one weak (sca3-1) and...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.106.080069

    authors: Hricová A,Quesada V,Micol JL

    更新日期:2006-07-01 00:00:00

  • Nitrogen Metabolism in Soybean Tissue Culture: II. Urea Utilization and Urease Synthesis Require Ni.

    abstract::Potassium citrate (10 mM, pH 6) inhibits the growth of cultured (Glycine max L.) cells when urea is the sole nitrogen source. Ureadependent citrate toxicity is overcome by three separate additions to the growth medium: (a) NH(4)Cl (20 mM); (b) high levels of MgCl(2) (10 mM) or CaCl(2) (5-10 mM); (c) low levels of NiSO...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.59.5.827

    authors: Polacco JC

    更新日期:1977-05-01 00:00:00

  • Dynamics of Endogenous Cytokinins during the Growth Cycle of a Hormone-Autotrophic Genetic Tumor Line of Tobacco.

    abstract::The profile of endogenous cytokinins in a genetic tumor line of tobacco, namely, Nicotiana glauca (Grah.) x Nicotiana langsdorffii (Weinm.), following 1 to 10 weeks of growth on solid medium was determined by radioimmunoassay. (3)H-labeled cytokinins of high specific activity were added during tissue extraction to cor...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.94.3.1084

    authors: Nandi SK,Palni LM,Parker CW

    更新日期:1990-11-01 00:00:00

  • Two young MicroRNAs originating from target duplication mediate nitrogen starvation adaptation via regulation of glucosinolate synthesis in Arabidopsis thaliana.

    abstract::Nitrogen is an essential macronutrient required for plant growth and development. A number of genes respond to nitrogen starvation conditions. However, the functions of most of these nitrogen starvation-responsive genes are unclear. Our recent survey suggested that many microRNAs (miRNAs) are responsive to nitrogen st...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.113.228635

    authors: He H,Liang G,Li Y,Wang F,Yu D

    更新日期:2014-02-01 00:00:00

  • Variations in leaf soluble amino acids and ammonium content in subtropical seagrasses related to salinity stress.

    abstract::A survey of leaf soluble amino acids was conducted for four subtropical seagrasses grown at several salinities. Proline functioned as an organic osmoticum in Halodule wrightii Aschers., Thallasia testudinum Banks ex Koenig, and Ruppia maritima L., while alanine functioned in an osmoregulatory capacity in Halophila eng...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.80.1.283

    authors: Pulich WM

    更新日期:1986-01-01 00:00:00

  • Key Traits and Genes Associate with Salinity Tolerance Independent from Vigor in Cultivated Sunflower.

    abstract::With rising food demands, crop production on salinized lands is increasingly necessary. Sunflower (Helianthus annuus), a moderately salt-tolerant crop, exhibits a tradeoff where more vigorous, high-performing genotypes have a greater proportional decline in biomass under salinity stress. Prior research has found devia...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.20.00873

    authors: Temme AA,Kerr KL,Masalia RR,Burke JM,Donovan LA

    更新日期:2020-10-01 00:00:00

  • Overproduction in Escherichia coli and Characterization of a Soybean Ferric Leghemoglobin Reductase.

    abstract::We previously cloned and sequenced a cDNA encoding soybean ferric leghemoglobin reductase (FLbR), an enzyme postulated to play an important role in maintaining leghemoglobin in a functional ferrous state in nitrogen-fixing root nodules. This cDNA was sub-cloned into an expression plasmid, pTrcHis C, and overexpressed ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.106.1.203

    authors: Ji L,Becana M,Sarath G,Shearman L,Klucas RV

    更新日期:1994-09-01 00:00:00

  • Cotyledon-Generated Auxin Is Required for Shade-Induced Hypocotyl Growth in Brassica rapa.

    abstract::Plant architecture is optimized for the local light environment. In response to foliar shade or neighbor proximity (low red to far-red light), some plant species exhibit shade-avoiding phenotypes, including increased stem and hypocotyl growth, which increases the likelihood of outgrowing competitor plants. If shade pe...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.114.241844

    authors: Procko C,Crenshaw CM,Ljung K,Noel JP,Chory J

    更新日期:2014-07-01 00:00:00

  • Isolation and Characterization of O-methyltransferases Involved in the Biosynthesis of Glaucine in Glaucium flavum.

    abstract::Transcriptome resources for the medicinal plant Glaucium flavum were searched for orthologs showing identity with characterized O-methyltransferases (OMTs) involved in benzylisoquinoline alkaloid biosynthesis. Seven recombinant proteins were functionally tested using the signature alkaloid substrates for six OMTs: nor...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.15.01240

    authors: Chang L,Hagel JM,Facchini PJ

    更新日期:2015-10-01 00:00:00

  • Structure-activity relationship in the auxin activity of mono-substituted phenylacetic acids.

    abstract::The analysis of substituent constants for the lipophilic and electronic factors in the auxin activity of substituted phenylacetic acids in elongation of coleoptile segments shows that these factors parallel those for the phenoxyacetic acids but assign reactivity in growth promotion to the meta position of phenylacetic...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.42.11.1519

    authors: Muir RM,Fujita T,Hansch C

    更新日期:1967-11-01 00:00:00

  • Iron-mediated control of the basic helix-loop-helix protein FER, a regulator of iron uptake in tomato.

    abstract::Root iron mobilization genes are induced by iron deficiency downstream of an unknown signaling mechanism. The FER gene, encoding a basic helix-loop-helix domain protein and putative transcription factor, is required for induction of iron mobilization genes in roots of tomato (Lycopersicon esculentum). To study upstrea...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.104.054270

    authors: Brumbarova T,Bauer P

    更新日期:2005-03-01 00:00:00

  • Heat treatment results in a loss of transgene-encoded activities in several tobacco lines.

    abstract::Heat treatment (37 degrees C) of transgenic tobacco (Nicotiana tabacum) plants led to a reversible reduction or complete loss of transgene-encoded activities in about 40% of 10 independent transformants carrying the luciferase-coding region fused to the 355 cauliflower mosaic virus or the soybean small subunit promote...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.115.3.939

    authors: Neumann K,Dröge-Laser W,Köhne S,Broer I

    更新日期:1997-11-01 00:00:00

  • Dynamic Maize Responses to Aphid Feeding Are Revealed by a Time Series of Transcriptomic and Metabolomic Assays.

    abstract::As a response to insect attack, maize (Zea mays) has inducible defenses that involve large changes in gene expression and metabolism. Piercing/sucking insects such as corn leaf aphid (Rhopalosiphum maidis) cause direct damage by acquiring phloem nutrients as well as indirect damage through the transmission of plant vi...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.15.01039

    authors: Tzin V,Fernandez-Pozo N,Richter A,Schmelz EA,Schoettner M,Schäfer M,Ahern KR,Meihls LN,Kaur H,Huffaker A,Mori N,Degenhardt J,Mueller LA,Jander G

    更新日期:2015-11-01 00:00:00

  • In Vivo and in Vitro Studies of Glucose-6-Phosphate Dehydrogenase from Barley Root Plastids in Relation to Reductant Supply for NO2- Assimilation.

    abstract::Pyridine nucleotide pools were measured in intact plastids from roots of barley (Hordeum vulgare L.) during the onset of NO2- assimilation and compared with the in vitro effect of the NADPH/NADP ratio on the activity of plastidic glucose-6-phosphate dehydrogenase (G6PDH, EC 1.1.1.49) from N-sufficient or N-starved roo...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.114.4.1413

    authors: Wright DP,Huppe HC,Turpin DH

    更新日期:1997-08-01 00:00:00