Immunocytolocalization of polygalacturonase in ripening tomato fruit.

Abstract:

:Using tissue blotting and immunocytolocalization, we have investigated the appearance and accumulation of polygalacturonase (PG) during tomato (Lycopersicon esculentum Mill.) fruit ripening. Results show that PG first appears in the collumella region followed by sequential appearance in the exopericarp and endopericarp, respectively. Detectable levels of PG were not present in the locular material containing seeds. This result indicates that PG synthesis initiates at the central collumella region of tomato fruit during ripening.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Tieman DM,Handa AK

doi

10.1104/pp.90.1.17

subject

Has Abstract

pub_date

1989-05-01 00:00:00

pages

17-20

issue

1

eissn

0032-0889

issn

1532-2548

journal_volume

90

pub_type

杂志文章
  • Senescence-induced, thylakoid-bound diisopropylfluorophosphate-binding protein in spinach : induction pattern, localization, and some properties.

    abstract::Changes in diisopropylfluorophosphate (DFP)-binding proteins during development and senescence of spinach (Spinacia oleracea) leaves were followed using [(3)H]DFP and sodium dodecylsulfate-polyacrylamide gel electrophoresis-fluorography. Experiments using a series of aging stages of leaves attached to plants and ones ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.90.1.338

    authors: Kawasaki S,Takeuchi J

    更新日期:1989-05-01 00:00:00

  • sym 13-A Gene Conditioning Ineffective Nodulation in Pisum sativum.

    abstract::Treatment of Pisum sativum (L.) cv. ;Sparkle' with ethyl methanesulfonic acid (EMS) produced a stable mutant, E135F, which forms small, white, ineffective nodules. These nodules exhibit histological zonation typical of an indeterminant nodule, e.g. meristematic, early symbiotic, late symbiotic, and senescent zones. Co...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.94.3.899

    authors: Kneen BE,Larue TA,Hirsch AM,Smith CA,Weeden NF

    更新日期:1990-11-01 00:00:00

  • Light Quality and Irradiance Level Interaction in the Control of Expression of Light-Harvesting Complex of Photosystem II: Pigments, Pigment-Proteins, and mRNA Accumulation.

    abstract::Effects of red and blue light at irradiances from 1.6 to 28.3 micromolar per square meter per second on chloroplast pigments, light-harvesting pigment-proteins associated with photosystem II, and the corresponding mRNA were evaluated in maize (Zea mays L.) plants (OP Golden Bantum) grown for 14 days under 14 hours lig...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.91.1.163

    authors: Eskins K,Westhoff P,Beremand PD

    更新日期:1989-09-01 00:00:00

  • Photocontrol of the Accumulation of Plastid Polypeptides during Greening of Tomato Cotyledons : Potentiation by a Pulse of Red Light.

    abstract::A pulse of red light acting through phytochrome accelerates the formation of chlorophyll upon subsequent transfer of dark-grown seedlings to continuous white light. Specific antibodies were used to follow the accumulation of representative subunits of the major photosynthetic complexes during greening of seedlings of ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.100.4.1934

    authors: Pauncz Y,Gepstein S,Horwitz BA

    更新日期:1992-12-01 00:00:00

  • Involvement of intracellular calcium in anaerobic gene expression and survival of maize seedlings.

    abstract::Ca-mediated processes are known to be involved in transducing many developmental, hormonal, and environmental cues in plant cells. In this study, the role of Ca in the perception of anoxic stress signals by maize (Zea mays L. cv B73) roots was assessed by studying the effect of various Ca antagonists on the induction ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.105.1.369

    authors: Subbaiah CC,Zhang J,Sachs MM

    更新日期:1994-05-01 00:00:00

  • Fatty acid activation in cyanobacteria mediated by acyl-acyl carrier protein synthetase enables fatty acid recycling.

    abstract::In cyanobacteria fatty acids destined for lipid synthesis can be synthesized de novo, but also exogenous free fatty acids from the culture medium can be directly incorporated into lipids. Activation of exogenous fatty acids is likely required prior to their utilization. To identify the enzymatic activity responsible f...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.109.148007

    authors: Kaczmarzyk D,Fulda M

    更新日期:2010-03-01 00:00:00

  • Second positive phototropism results from coordinated co-action of the phototropins and cryptochromes.

    abstract::Phototropism and hypocotyl growth inhibition are modulated by the coaction of different blue-light photoreceptors and their signaling pathways. How seedlings integrate the activities of the different blue-light photoreceptors to coordinate these hypocotyl growth responses is still unclear. We have used time-lapse imag...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.102.018481

    authors: Whippo CW,Hangarter RP

    更新日期:2003-07-01 00:00:00

  • A survey of the pectic content of nonlignified monocot cell walls.

    abstract::The primary cell walls of graminaceous monocots were known to have a low content of pectin compared to those of dicots, but it was uncertain how widespread this feature was within the monocots as a whole. Nonlignified cell walls were therefore prepared from 33 monocot species for determination of their pectin content....

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.88.2.309

    authors: Jarvis MC,Forsyth W,Duncan HJ

    更新日期:1988-10-01 00:00:00

  • Differences in PpAAT1 Activity in High- and Low-Aroma Peach Varieties Affect γ-Decalactone Production.

    abstract::Aroma contributes to the unique flavors of fruits and is important for fruit quality evaluation. Among the many volatiles in peach (Prunus persica) fruits, γ-decalactone has the greatest contribution to the characteristic peach aroma. Some peach cultivars have γ-decalactone contents that are too low to detect. Compari...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.19.00964

    authors: Peng B,Yu M,Zhang B,Xu J,Ma R

    更新日期:2020-04-01 00:00:00

  • Phosphorylation of serine-15 of maize leaf sucrose synthase. Occurrence in vivo and possible regulatory significance.

    abstract::Experiments were conducted to determine whether sucrose synthase (SuSy) was phosphorylated in the elongation zone of maize (Zea mays L.) leaves. The approximately 90-kD subunit of SuSy was 32P-labeled on seryl residue(s) when excised shoots were fed [32P]orthophosphate. Both isoforms of SuSy (the SS1 and SS2 proteins)...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.112.2.793

    authors: Huber SC,Huber JL,Liao PC,Gage DA,McMichael RW Jr,Chourey PS,Hannah LC,Koch K

    更新日期:1996-10-01 00:00:00

  • Ion transport processes in corn mitochondria : I. Effect of the local anesthetic dibucaine.

    abstract::The local anesthetic dibucaine inhibited respiration-dependent contraction mediated by the K(+)/H(+) antiport system of isolated corn mitochondria. Respiration declined concurrently. Nigericin, an exogenous K(+)/H(+) exchanger, restored ion efflux in dibucaine-blocked corn mitochondria. It was concluded that dibucaine...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.77.4.909

    authors: Klein RR,Koeppe DE

    更新日期:1985-04-01 00:00:00

  • Regulation of microbe-associated molecular pattern-induced hypersensitive cell death, phytoalexin production, and defense gene expression by calcineurin B-like protein-interacting protein kinases, OsCIPK14/15, in rice cultured cells.

    abstract::Although cytosolic free Ca(2+) mobilization induced by microbe/pathogen-associated molecular patterns is postulated to play a pivotal role in innate immunity in plants, the molecular links between Ca(2+) and downstream defense responses still remain largely unknown. Calcineurin B-like proteins (CBLs) act as Ca(2+) sen...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.109.151852

    authors: Kurusu T,Hamada J,Nokajima H,Kitagawa Y,Kiyoduka M,Takahashi A,Hanamata S,Ohno R,Hayashi T,Okada K,Koga J,Hirochika H,Yamane H,Kuchitsu K

    更新日期:2010-06-01 00:00:00

  • Isolation of Guard Cell Protoplasts from Mechanically Prepared Epidermis of Vicia faba Leaves.

    abstract::A method for isolating guard cell protoplasts (GCP) from mechanically prepared epidermis of Vicia faba is described. Epidermis was prepared by homogenizing leaves in a Waring blender in a solution of 10% Ficoll, 5 millimolar CaCl(2), and 0.1% polyvinylpyrrolidone 40 (PVP). Attached mesophyll and epidermal cells were r...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.90.4.1382

    authors: Kruse T,Tallman G,Zeiger E

    更新日期:1989-08-01 00:00:00

  • Isolation of a cDNA encoding a granule-bound 152-kilodalton starch-branching enzyme in wheat.

    abstract::Screening of a wheat (Triticum aestivum) cDNA library for starch-branching enzyme I (SBEI) genes combined with 5'-rapid amplification of cDNA ends resulted in isolation of a 4,563-bp composite cDNA, Sbe1c. Based on sequence alignment to characterized SBEI cDNA clones isolated from plants, the SBEIc predicted from the ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.124.1.253

    authors: Båga M,Nair RB,Repellin A,Scoles GJ,Chibbar RN

    更新日期:2000-09-01 00:00:00

  • Foxtail Mosaic Virus-Induced Gene Silencing in Monocot Plants.

    abstract::Virus-induced gene silencing (VIGS) is a powerful technique to study gene function in plants. However, very few VIGS vectors are available for monocot plants. Here we report that Foxtail mosaic virus (FoMV) can be engineered as an effective VIGS system to induce efficient silencing of endogenous genes in monocot plant...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.16.00010

    authors: Liu N,Xie K,Jia Q,Zhao J,Chen T,Li H,Wei X,Diao X,Hong Y,Liu Y

    更新日期:2016-07-01 00:00:00

  • Fifty years of progress in water relations research.

    abstract::Many of the basic concepts dealing with soil and plant water relationships were in existence 50 years ago, but were inadequately presented in the textbooks of that time. There has been a marked increase in the amount of work done in this field during recent decades, but much of it involves advances in understanding th...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.54.4.463

    authors: Kramer PJ

    更新日期:1974-10-01 00:00:00

  • Rice ROOT ARCHITECTURE ASSOCIATED1 binds the proteasome subunit RPT4 and is degraded in a D-box and proteasome-dependent manner.

    abstract::Root growth is mainly determined by cell division and subsequent elongation in the root apical area. Components regulating cell division in root meristematic cells are largely unknown. Previous studies have identified rice (Oryza sativa) ROOT ARCHITECTURE ASSOCIATED1 (OsRAA1) as a regulator in root development. Yet, t...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.108.125294

    authors: Han Y,Cao H,Jiang J,Xu Y,Du J,Wang X,Yuan M,Wang Z,Xu Z,Chong K

    更新日期:2008-10-01 00:00:00

  • Alteration in cell permeability as a mechanism of action of certain quinone pesticides.

    abstract::The permeability of the Chlorella pyrenoidosa membrane was studied by following the efflux of (14)C-intracellular material from cells which had been allowed to incorporate (14)CO(2) photosynthetically. It was observed that the efflux increased upon treatment with low concentrations (3-30 muM) of 2, 3-dichloro-1, 4-nap...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.51.2.363

    authors: Sikka HC,Saxena J,Zweig G

    更新日期:1973-02-01 00:00:00

  • Histochemical Compartmentation of Photosynthesis in the Crassulacean Acid Metabolism Plant Crassula falcata.

    abstract::The succulent leaf of the obligate Crassulacean acid metabolism plant Crassula falcata comprises two distinct types of parenchyma. The peripheral tissue is dark green, whereas the central tissue is relatively colorless. We have investigated whether the conventional interpretation of Crassulacean acid metabolism-simply...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.88.3.633

    authors: Springer SA,Outlaw WH

    更新日期:1988-11-01 00:00:00

  • Studies on Cation-induced Thylakoid Membrane Stacking, Fluorescence Yield, and Photochemical Efficiency.

    abstract::Trypsin digestion of photosynthetic membranes isolated from spinach (Spinacia oleracea L.) leaves eliminates the cation stimulation of chlorophyll fluorescence. High concentrations of cations protect the fluorescence yield against trypsin digestion, and the cation specificity for this protection closely resembles that...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.62.6.879

    authors: Jennings RC,Forti G,Gerola PD,Garlaschi FM

    更新日期:1978-12-01 00:00:00

  • Loss-of-function mutations of ROOT HAIR DEFECTIVE3 suppress root waving, skewing, and epidermal cell file rotation in Arabidopsis.

    abstract::Wild-type Arabidopsis (Arabidopsis thaliana L. Heynh.) roots growing on a tilted surface of impenetrable hard-agar media adopt a wave-like pattern and tend to skew to the right of the gravity vector (when viewed from the back of the plate through the medium). Reversible root-tip rotation often accompanies the clockwis...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.105.059774

    authors: Yuen CY,Sedbrook JC,Perrin RM,Carroll KL,Masson PH

    更新日期:2005-06-01 00:00:00

  • Kinetic Variance of Ribulose-1,5-bisphosphate Carboxylase/Oxygenase Isolated from Diverse Taxonomic Sources : II. Analysis by Two Dual Label Methods.

    abstract::Two dual label methods were used to investigate kinetic variability of ribulose 1,5-bisphosphate (RuBP) carboxylase/oxygenase (EC 4.1.1.39). In addition to using [1-(14)C,5-(3)H]RuBP (method 1), we describe here the detailed assay with (14)CO(2) and [5-(3)H]RuBP (method 2), which generates [(3)H,(14)C]3-phosphoglyceri...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.75.3.645

    authors: Kent SS,Tomany MJ

    更新日期:1984-07-01 00:00:00

  • Chlorophyll Formation and Photosynthetic Competence in Euglena During Light-Induced Chloroplast Development in the Presence of 3, (3,4-dichlorophenyl) 1,1-Dimethyl Urea (DCMU).

    abstract::The possibility that photosynthetic competence is gratuitous for light-induced chloroplast development in Euglena gracilis var. bacillaris was examined by incubating dark-grown resting cells in the light with DCMU, an inhibitor of photosynthesis. Under these conditions photosynthetic carbon dioxide fixation was inhibi...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.42.12.1716

    authors: Schiff JA,Zeldin MH,Rubman J

    更新日期:1967-12-01 00:00:00

  • Complementation of sugary-1 phenotype in rice endosperm with the wheat isoamylase1 gene supports a direct role for isoamylase1 in amylopectin biosynthesis.

    abstract::To examine the role of isoamylase1 (ISA1) in amylopectin biosynthesis in plants, a genomic DNA fragment from Aegilops tauschii was introduced into the ISA1-deficient rice (Oryza sativa) sugary-1 mutant line EM914, in which endosperm starch is completely replaced by phytoglycogen. A. tauschii is the D genome donor of w...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.104.051359

    authors: Kubo A,Rahman S,Utsumi Y,Li Z,Mukai Y,Yamamoto M,Ugaki M,Harada K,Satoh H,Konik-Rose C,Morell M,Nakamura Y

    更新日期:2005-01-01 00:00:00

  • Correction of Freezing Artifacts in the Application of Electron Paramagnetic Resonance for Quantification of the Reduction of Ferric Hydroxamate by Chlorella vulgaris.

    abstract::Electron paramagnetic resonance has been used to quantitate reduction of ferric ion bound to hydroxamate siderophores by iron-stressed fungi (DJ Ecker et al. 1982 J Biol Chem 257:8623-8626) and by iron-stressed Chlorella vulgaris (ATCC 11468) (FCT Allnutt, WD Bonner 1984 J Plant Nutr 7: 427-435). Application of this t...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.83.4.739

    authors: Allnutt FC,Bonner WD

    更新日期:1987-04-01 00:00:00

  • Photosynthetic carbon metabolism of isolated corn chloroplasts.

    abstract::Chloroplasts have been isolated from 4- to 6-day-old corn (Zea mays) leaves capable of assimilating 45 micromoles CO(2) per milligram chlorophyll per hour. The effects of various factors such as inorganic phosphate, reducing agents, inhibitors, intermediates of the photosynthetic carbon reduction cycle, organic acids,...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.49.4.607

    authors: O'neal D,Hew CS,Latzko E,Gibbs M

    更新日期:1972-04-01 00:00:00

  • Gibberellins promote trichome formation by Up-regulating GLABROUS1 in arabidopsis

    abstract::Trichome development is dependent on gibberellin (GA) signaling in Arabidopsis thaliana. Using the GA-deficient mutant ga1-3, the GA-response mutant spy-5, and uniconazol (a GA-biosynthesis inhibitor), we show that the GA level response correlates positively with both trichome number and trichome branch number. Two ge...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.117.2.375

    authors: Perazza D,Vachon G,Herzog M

    更新日期:1998-06-01 00:00:00

  • Dehydroascorbate influences the plant cell cycle through a glutathione-independent reduction mechanism.

    abstract::Glutathione is generally accepted as the principal electron donor for dehydroascorbate (DHA) reduction. Moreover, both glutathione and DHA affect cell cycle progression in plant cells. But other mechanisms for DHA reduction have been proposed. To investigate the connection between DHA and glutathione, we have evaluate...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.103.033548

    authors: Potters G,Horemans N,Bellone S,Caubergs RJ,Trost P,Guisez Y,Asard H

    更新日期:2004-04-01 00:00:00

  • Role of the low-molecular-weight subunits PetL, PetG, and PetN in assembly, stability, and dimerization of the cytochrome b6f complex in tobacco.

    abstract::The cytochrome b(6)f (Cyt b(6)f) complex in flowering plants contains nine conserved subunits, of which three, PetG, PetL, and PetN, are bitopic plastid-encoded low-molecular-weight proteins of largely unknown function. Homoplastomic knockout lines of the three genes have been generated in tobacco (Nicotiana tabacum '...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.107.100131

    authors: Schwenkert S,Legen J,Takami T,Shikanai T,Herrmann RG,Meurer J

    更新日期:2007-08-01 00:00:00

  • Chill-Induced Changes in the Activity and Abundance of the Vacuolar Proton-Pumping Pyrophosphatase from Mung Bean Hypocotyls.

    abstract::Changes in the properties of extractable vacuolar H+-pumping pyrophosphatase (V-PPase) and vacuolar ATPase activities in chilling-sensitive seedlings of mung bean (Vigna radiata) were investigated. Following chilling at 4[deg]C for 48 h, both hydrolytic and proton-pumping activities of the V-PPase increased 1.5- to 2-...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.109.2.659

    authors: Darley CP,Davies JM,Sanders D

    更新日期:1995-10-01 00:00:00