Abstract：
:To examine the role of isoamylase1 (ISA1) in amylopectin biosynthesis in plants, a genomic DNA fragment from Aegilops tauschii was introduced into the ISA1-deficient rice (Oryza sativa) sugary-1 mutant line EM914, in which endosperm starch is completely replaced by phytoglycogen. A. tauschii is the D genome donor of wheat (Triticum aestivum), and the introduced fragment effectively included the gene for ISA1 for wheat (TaISA1) that was encoded on the D genome. In TaISA1-expressing rice endosperm, phytoglycogen synthesis was substantially replaced by starch synthesis, leaving only residual levels of phytoglycogen. The levels of residual phytoglycogen present were inversely proportional to the expression level of the TaISA1 protein, although the level of pullulanase that had been reduced in EM914 was restored to the same level as that in the wild type. Small but significant differences were found in the amylopectin chain-length distribution, gelatinization temperatures, and A-type x-ray diffraction patterns of the starches from lines expressing TaISA1 when compared with wild-type rice starch, although in the first two parameters, the effect was proportional to the expression level of TaISA. The impact of expression levels of ISA1 on starch structure and properties provides support for the view that ISA1 is directly involved in the synthesis of amylopectin.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Kubo A,Rahman S,Utsumi Y,Li Z,Mukai Y,Yamamoto M,Ugaki M,Harada K,Satoh H,Konik-Rose C,Morell M,Nakamura Ydoi
10.1104/pp.104.051359subject
Has Abstractpub_date
2005-01-01 00:00:00pages
43-56issue
1eissn
0032-0889issn
1532-2548pii
pp.104.051359journal_volume
137pub_type
杂志文章相关文献
PLANT PHYSIOLOGY文献大全abstract：:Phosphorus (P) is a crucial nutrient for plant growth, but its availability to roots is limited in soil. Arbuscular mycorrhizal (AM) symbiosis is a promising strategy for improving plant P acquisition. However, P fertilizer reduces fungal colonization (P inhibition) and compromises mycorrhizal P uptake, warranting stu...
journal_title：Plant physiology
pub_type： 杂志文章
doi：10.1104/pp.16.00127
更新日期：2016-05-01 00:00:00
abstract：:A depolarization-activated K(+) channel capable of carrying the large K(+) currents that flow from shrinking cells during movements of Samanea saman leaflets has been described in the plasmalemma of Samanea motor cell protoplasts (N Moran et al [1988] Plant Physiol 88:643-648). We now characterize this channel in grea...
journal_title：Plant physiology
pub_type： 杂志文章
doi：10.1104/pp.94.2.424
更新日期：1990-10-01 00:00:00
abstract：:Incubation under water in a 30 C/14-hour or 12 C/10-hour photoperiod caused the CO(2) compensation points of 10 aquatic macrophytes to decrease below 25 or increase above 50 microliters CO(2) per liter, respectively. Submerged and aerial leaves of two amphibious angiosperms (Myriophyllum brasiliense and Proserpinaca p...
journal_title：Plant physiology
pub_type： 杂志文章
doi：10.1104/pp.67.2.335
更新日期：1981-02-01 00:00:00
abstract：:The cytochrome b(6)f (Cyt b(6)f) complex in flowering plants contains nine conserved subunits, of which three, PetG, PetL, and PetN, are bitopic plastid-encoded low-molecular-weight proteins of largely unknown function. Homoplastomic knockout lines of the three genes have been generated in tobacco (Nicotiana tabacum '...
journal_title：Plant physiology
pub_type： 杂志文章
doi：10.1104/pp.107.100131
更新日期：2007-08-01 00:00:00
abstract：:Nitrate releases seed dormancy in Arabidopsis (Arabidopsis thaliana) Columbia accession seeds in part by reducing abscisic acid (ABA) levels. Nitrate led to lower levels of ABA in imbibed seeds when included in the germination medium (exogenous nitrate). Nitrate also reduced ABA levels in dry seeds when provided to th...
journal_title：Plant physiology
pub_type： 杂志文章
doi：10.1104/pp.108.126938
更新日期：2009-02-01 00:00:00
abstract：:Cytidine deoxyriboside (Cdr), uridine deoxyriboside (Udr), and guanosine deoxyriboside (Gdr), induce quantitative bleaching of the fronds of Lemna minor (duckweed) during growth in continuous light on photoheterotrophic medium. Cdr-induced bleaching is not accompanied by a reduction in frond multiplication rate, but U...
journal_title：Plant physiology
pub_type： 杂志文章
doi：10.1104/pp.61.6.989
更新日期：1978-06-01 00:00:00
abstract：:We have isolated the plasma membrane H+-ATPase in a phosphorylated form from spinach (Spinacia oleracea L.) leaf tissue incubated with fusicoccin, a fungal toxin that induces irreversible binding of 14-3-3 protein to the C terminus of the H+-ATPase, thus activating H+ pumping. We have identified threonine-948, the sec...
journal_title：Plant physiology
pub_type： 杂志文章
doi：10.1104/pp.118.2.551
更新日期：1998-10-01 00:00:00
abstract：:As a response to insect attack, maize (Zea mays) has inducible defenses that involve large changes in gene expression and metabolism. Piercing/sucking insects such as corn leaf aphid (Rhopalosiphum maidis) cause direct damage by acquiring phloem nutrients as well as indirect damage through the transmission of plant vi...
journal_title：Plant physiology
pub_type： 杂志文章
doi：10.1104/pp.15.01039
更新日期：2015-11-01 00:00:00
abstract：:The effect of ozone was studied on the peroxidase activity from various compartments of Sedum album leaves (epidermis, intercellular fluid, residual cell material, and total cell material). The greatest increase following a 2-hour ozone exposure (0.4 microliters O(3) per liter) was observed in extracellular peroxidase...
journal_title：Plant physiology
pub_type： 杂志文章
doi：10.1104/pp.74.4.846
更新日期：1984-04-01 00:00:00
abstract：:We used tissue printing and specific immunostaining to examine the localization of the alternative oxidase (AOX) protein in correlation with measurements of AOX capacity. Selected root and hypocotyl regions were analyzed during the first 14 d of growth. It is shown that AOX protein is localized in the apical meristem ...
journal_title：Plant physiology
pub_type： 杂志文章
doi：10.1104/pp.115.4.1499
更新日期：1997-12-01 00:00:00
abstract：:NADP-glyceraldehyde-3-P dehydrogenase of spinach (Spinacia oleracea) chloroplasts was activated by thioredoxin that was reduced either photochemically with ferredoxin and ferredoxin-thioredoxin reductase or chemically with dithiothreitol. The activation process that was observed with the soluble protein fraction from ...
journal_title：Plant physiology
pub_type： 杂志文章
doi：10.1104/pp.61.4.669
更新日期：1978-04-01 00:00:00
abstract：:Mildly disrupted cells of Chlamydomonas reinhardi Dangeard were incubated in a reaction medium containing glycolate, ferricyanide, and cupric ions, and then processed for electron microscopy. As a result of the cytochemical treatment, an electron opaque product was deposited specifically in the outer compartment of mi...
journal_title：Plant physiology
pub_type： 杂志文章
doi：10.1104/pp.58.3.315
更新日期：1976-09-01 00:00:00
abstract：:Plant roots show a particularly high variation in their morphological response to different nutrient deficiencies. Although such changes often determine the nutrient efficiency or stress tolerance of plants, it is surprising that a comprehensive and comparative analysis of root morphological responses to different nut...
journal_title：Plant physiology
pub_type： 杂志文章
doi：10.1104/pp.113.218453
更新日期：2013-09-01 00:00:00
abstract：:Intact peroxisomes were prepared from green leaves of a number of C(3) species, both monocots and dicots. A protoplast extract from which chloroplasts have been removed by a 1-minute 10,000g centrifugation was applied to a step gradient of 5, 15, 30, and 45% Percoll containing 0.5 molar sucrose, 0.1% BSA, and 25 milli...
journal_title：Plant physiology
pub_type： 杂志文章
doi：10.1104/pp.70.4.1213
更新日期：1982-10-01 00:00:00
abstract：:Rabbit antiserum against beta-amylase isolated from germinating seeds of rice was produced, and its specific cross-reactivity with beta-amylase was confirmed by means of Ouchterlony double immunodiffusion and immunoelectrophoresis procedures. The cellular localization of beta-amylase was studied by indirect fluorescen...
journal_title：Plant physiology
pub_type： 杂志文章
doi：10.1104/pp.64.2.337
更新日期：1979-08-01 00:00:00
abstract：:The BEL1-like family of transcription factors is ubiquitous in plants and plays important roles in regulating development. They function in tandem with KNOTTED1 types to bind to a double TTGAC motif in the upstream sequence of target genes. StBEL5 of potato (Solanum tuberosum) functions as a mobile RNA signal that is ...
journal_title：Plant physiology
pub_type： 杂志文章
doi：10.1104/pp.15.01314
更新日期：2016-01-01 00:00:00
abstract：:The interaction between mannan polysaccharides and cellulose microfibrils contributes to cell wall properties in some vascular plants, but the molecular arrangement of mannan in the cell wall and the nature of the molecular bonding between mannan and cellulose remain unknown. Previous studies have shown that mannan is...
journal_title：Plant physiology
pub_type： 杂志文章
doi：10.1104/pp.18.00709
更新日期：2018-11-01 00:00:00
abstract：:It has been shown previously that an increase in ribulose diphosphate carboxylase activity occurs upon brief illumination of leaves of dark-grown Zea mays plants; an increase in ribose 5-phosphate isomerase occurs after prolonged illumination. Both of these responses to illumination are inhibited by chloramphenicol.Th...
journal_title：Plant physiology
pub_type： 杂志文章
doi：10.1104/pp.43.2.188
更新日期：1968-02-01 00:00:00
abstract：:The structure of a glucuronoarabinoxylan in Zea mays L. (hybrid B73 x Mo17) shoot cell walls has been studied. The water-insoluble fraction of Zea shoot cell walls, pretreated with purified Bacillus subtilis (1 --> 3), (1 --> 4)-beta-d-glucan 4-glucanohydrolase, was treated with purified B. subtilis endo-(1 --> 4)-bet...
journal_title：Plant physiology
pub_type： 杂志文章
doi：10.1104/pp.75.3.759
更新日期：1984-07-01 00:00:00
abstract：:Glyceraldehyde 3-phosphate dehydrogenases (EC 1.2.1.12 and 1.2.1.13) have been purified from the seed, root, etiolated, and green shoot of peas (Pisum sativum). These enzymes are tetramers of 140,000 daltons, with subunits of 35,000 daltons. The enzymes differ in isoelectric point. The seed enzyme has a pI of 5.1, and...
journal_title：Plant physiology
pub_type： 杂志文章
doi：10.1104/pp.54.3.312
更新日期：1974-09-01 00:00:00
abstract：:In eukaryotic cells, histones are subject to a large number of posttranslational modifications whose sequential or combinatorial action affects chromatin structure and genome function. We identified acetylation at Lys-36 in histone H3 (H3K36ac) as a new chromatin modification in plants. The H3K36ac modification is evo...
journal_title：Plant physiology
pub_type： 杂志文章
doi：10.1104/pp.15.01744
更新日期：2016-03-01 00:00:00
abstract：:The Intracellular Na(+) concentration in the halotolerant alga Dunaliella salina was measured in intact cells by (23)Na-NMR spectroscopy, utilizing the dysprosium tripolyphosphate complex as a sodium shift reagent, and was found to be 88 +/- 28 millimolar. Intracellular sodium ion content and intracellular volume were...
journal_title：Plant physiology
pub_type： 杂志文章
doi：10.1104/pp.87.4.813
更新日期：1988-08-01 00:00:00
abstract：:Changes in acyl lipids and pigments during leaf development in a virescens barley mutant (M) and the normal (N) were studied. Apical 3-cm leaf segments were extracted with chloroform-methanol, the extracts were purified on Sephadex G-25 columns, and the polar lipids were separated on two-dimensional-thin layer chromat...
journal_title：Plant physiology
pub_type： 杂志文章
doi：10.1104/pp.67.4.646
更新日期：1981-04-01 00:00:00
abstract：:Three fractions (male germ unit [MGU]-rich, cytoplasmic-particulate and water-soluble proteins) were isolated from pollen of Plumbago zeylanica L. Proteins were extracted using a phenol procedure and polypeptide patterns were compared on one- and two-dimensional polyacrylamide gels. The MGU-rich fraction contains the ...
journal_title：Plant physiology
pub_type： 杂志文章
doi：10.1104/pp.88.3.764
更新日期：1988-11-01 00:00:00
abstract：:After incubation for 1 hr with (75)Se-selenate, excised roots of Astragalus crotalariae, a selenium-accumulating species, and A. lentiginosus, a nonaccumulator, had absorbed radioactivity to levels well over the external concentration. About 98% of the radioactivity was ethanol-soluble, and when analyzed by column and...
journal_title：Plant physiology
pub_type： 杂志文章
doi：10.1104/pp.44.6.893
更新日期：1969-06-01 00:00:00
abstract：:Treatment of Pisum sativum (L.) cv. ;Sparkle' with ethyl methanesulfonic acid (EMS) produced a stable mutant, E135F, which forms small, white, ineffective nodules. These nodules exhibit histological zonation typical of an indeterminant nodule, e.g. meristematic, early symbiotic, late symbiotic, and senescent zones. Co...
journal_title：Plant physiology
pub_type： 杂志文章
doi：10.1104/pp.94.3.899
更新日期：1990-11-01 00:00:00
abstract：:Preincubation of wheat (Triticum aestivum L. Thell.) seedlings in a nutrient solution containing low doses of aluminum (0.5 microgram per milliliter for tolerant cultivar Atlas 66 and 0.1 microgram per milliliter for the sensitive cultivar Grana) enabled substantial root regrowth of varieties grown in a lethal aluminu...
journal_title：Plant physiology
pub_type： 杂志文章
doi：10.1104/pp.76.3.551
更新日期：1984-11-01 00:00:00
abstract：:Previous investigations suggested that specific auxin spatial distribution due to auxin movements to particular embryonic regions was important for normal embryonic pattern formation. To gain information on the molecular mechanism(s) by which auxin acts to direct pattern formation in specific embryonic regions, the ro...
journal_title：Plant physiology
pub_type： 杂志文章
doi：10.1104/pp.013466
更新日期：2003-03-01 00:00:00
abstract：:Major storage reserves of Arabidopsis (Arabidopsis thaliana) seeds are triacylglycerols (seed oils) and proteins. Seed oil content is severely reduced for the regulatory mutant wrinkled1 (wri1-1; At3g54320) and for a double mutant in two isoforms of plastidic pyruvate kinase (pkpbeta(1)pkpalpha; At5g52920 and At3g2296...
journal_title：Plant physiology
pub_type： 杂志文章
doi：10.1104/pp.109.144121
更新日期：2009-11-01 00:00:00
abstract：:Meristematic tissues from rye (Secale cereale) and oat (Avena sativa) were studied in an isothermal calorimeter at -3 degrees C. When the frozen tissue was placed in the calorimeter, the pressure increased within 4 d to 25 and 9 kPa above ambient pressure in the sample vessels containing crowns of rye and oat, respect...
journal_title：Plant physiology
pub_type： 杂志文章
doi：10.1104/pp.122.3.861
更新日期：2000-03-01 00:00:00
