Foxtail Mosaic Virus-Induced Gene Silencing in Monocot Plants.

Abstract:

:Virus-induced gene silencing (VIGS) is a powerful technique to study gene function in plants. However, very few VIGS vectors are available for monocot plants. Here we report that Foxtail mosaic virus (FoMV) can be engineered as an effective VIGS system to induce efficient silencing of endogenous genes in monocot plants including barley (Hordeum vulgare L.), wheat (Triticum aestivum) and foxtail millet (Setaria italica). This is evidenced by FoMV-based silencing of phytoene desaturase (PDS) and magnesium chelatase in barley, of PDS and Cloroplastos alterados1 in foxtail millet and wheat, and of an additional gene IspH in foxtail millet. Silencing of these genes resulted in photobleached or chlorosis phenotypes in barley, wheat, and foxtail millet. Furthermore, our FoMV-based gene silencing is the first VIGS system reported for foxtail millet, an important C4 model plant. It may provide an efficient toolbox for high-throughput functional genomics in economically important monocot crops.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Liu N,Xie K,Jia Q,Zhao J,Chen T,Li H,Wei X,Diao X,Hong Y,Liu Y

doi

10.1104/pp.16.00010

subject

Has Abstract

pub_date

2016-07-01 00:00:00

pages

1801-7

issue

3

eissn

0032-0889

issn

1532-2548

pii

pp.16.00010

journal_volume

171

pub_type

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