Decreased cell wall digestibility in canola transformed with chimeric tyrosine decarboxylase genes from opium poppy

Abstract:

:Tyrosine decarboxylase (TYDC) is a common plant enzyme involved in the biosynthesis of numerous secondary metabolites, including hydroxycinnamic acid amides. Although a definite function has not yet been determined, amides have been proposed to form a physical barrier against pathogens because they are usually found as integral cell wall components. Canola (Brassica napus) was independently transformed with chimeric genes (35S::TYDC1 and 35S::TYDC2) under the transcriptional control of the cauliflower mosaic virus 35S promoter, and encoding two TYDC isoforms from opium poppy (Papaver somniferum). All T0 plants displayed a suppressed level of wild-type TYDC activity, and transgene mRNAs were not detected. Silencing of 35S::TYDC1 was overcome in the T1 progeny of self-pollinated T0 plants, since high levels of TYDC1 mRNAs were detected, and TYDC activity increased up to 4-fold compared with wild-type levels. However, TYDC1 mRNA levels decreased in T2 plants and were not detected in the T3 progeny. TYDC activity also gradually declined in T2 and T3 plants to nearly wild-type levels. In contrast, silencing of 35S::TYDC2 was maintained through four consecutive generations. T1 plants with a 3- to 4-fold increase in wild-type TYDC activity showed a 30% decrease in cellular tyrosine pools and a 2-fold increase in cell wall-bound tyramine compared with wild-type plants. An increase in cell wall-bound aromatic compounds was also detected in these T1 plants by ultraviolet autofluorescence microscopy. The relative digestibility of cell walls measured by protoplast release efficiency was inversely related to the level of TYDC activity.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Facchini PJ,Yu M,Penzes-Yost C

doi

10.1104/pp.120.3.653

subject

Has Abstract

pub_date

1999-07-01 00:00:00

pages

653-64

issue

3

eissn

0032-0889

issn

1532-2548

journal_volume

120

pub_type

杂志文章
  • Physiological studies with isolated leaf cells: early products of photosynthesis and their metabolic interconversions.

    abstract::A number of plants have been surveyed with respect to isolation by mild grinding in large quantities of leaf cells. The extent of recovery of mesophyll cells per unit leaf area was found to vary with plant species and the method of grinding. Greater than 70% recovery was obtained from the leaves of Canna indica L., Cr...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.54.4.569

    authors: Kulandaivelu G,Gnanam A

    更新日期:1974-10-01 00:00:00

  • Identification of 3-(O-beta-Glucosyl)-2-Indolone-3-Acetylaspartic Acid as a New Indole-3-Acetic Acid Metabolite in Vicia Seedlings.

    abstract::A new indole-3-acetic acid metabolite was isolated from broad bean (Vicia faba L. cv Chukyo) seedlings. It was a conjugate of dioxindole-3-acetic acid, aspartic acid, and glucose and was identified as 3-(O-beta-glucosyl)-2-indolone-3-acetylaspartic acid (molecular weight 484) from ultraviolet, infrared, nuclear magnet...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.79.3.667

    authors: Tsurumi S,Wada S

    更新日期:1985-11-01 00:00:00

  • Increased ratio of electron transport to net assimilation rate supports elevated isoprenoid emission rate in eucalypts under drought.

    abstract::Plants undergoing heat and low-CO2 stresses emit large amounts of volatile isoprenoids compared with those in stress-free conditions. One hypothesis posits that the balance between reducing power availability and its use in carbon assimilation determines constitutive isoprenoid emission rates in plants and potentially...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.114.246207

    authors: Dani KG,Jamie IM,Prentice IC,Atwell BJ

    更新日期:2014-10-01 00:00:00

  • Gibberellins promote trichome formation by Up-regulating GLABROUS1 in arabidopsis

    abstract::Trichome development is dependent on gibberellin (GA) signaling in Arabidopsis thaliana. Using the GA-deficient mutant ga1-3, the GA-response mutant spy-5, and uniconazol (a GA-biosynthesis inhibitor), we show that the GA level response correlates positively with both trichome number and trichome branch number. Two ge...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.117.2.375

    authors: Perazza D,Vachon G,Herzog M

    更新日期:1998-06-01 00:00:00

  • The biochemical and molecular basis for the divergent patterns in the biosynthesis of terpenes and phenylpropenes in the peltate glands of three cultivars of basil.

    abstract::Surface glandular trichomes distributed throughout the aerial parts of sweet basil (Ocimum basilicum) produce and store monoterpene, sesquiterpene, and phenylpropene volatiles. Three distinct basil chemotypes were used to examine the molecular mechanisms underlying the divergence in their monoterpene and sesquiterpene...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.104.051318

    authors: Iijima Y,Davidovich-Rikanati R,Fridman E,Gang DR,Bar E,Lewinsohn E,Pichersky E

    更新日期:2004-11-01 00:00:00

  • Termination of nutrient import and development of vein loading capacity in albino tobacco leaves.

    abstract::The sink-source conversion in developing leaves of tobacco (Nicotiana tabacum L.) was studied to determine whether import termination is caused by the onset of export or is related to achievement of positive carbon balance. Albino shoots were grown in vitro and grafted to detopped stems of green tobacco plants. Termin...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.76.1.45

    authors: Turgeon R

    更新日期:1984-09-01 00:00:00

  • Further Characterization on the Transport Property of Plasmalemma NADH Oxidation System in Isolated Corn Root Protoplasts.

    abstract::Recent experiments show that exogenous NADH increases the O(2) consumption and uptake of inorganic ions into isolated corn (Zea mays L. Pioneer Hybrid 3320) root protoplasts (Lin 1982, Proc Natl Acad Sci USA 79: 3773-3776). A mild treatment of protoplasts with trypsin released most of the NADH oxidation system from th...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.74.2.219

    authors: Lin W

    更新日期:1984-02-01 00:00:00

  • Independent action of ELF3 and phyB to control hypocotyl elongation and flowering time.

    abstract::Light regulates various aspects of plant growth, and the photoreceptor phytochrome B (phyB) mediates many responses to red light. In a screen for Arabidopsis mutants with phenotypes similar to those of phyB mutants, we isolated two new elf3 mutants. One has weaker morphological phenotypes than previously identified el...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.122.4.1149

    authors: Reed JW,Nagpal P,Bastow RM,Solomon KS,Dowson-Day MJ,Elumalai RP,Millar AJ

    更新日期:2000-04-01 00:00:00

  • Oxygen-18 and deuterium labeling studies of choline oxidation by spinach and sugar beet.

    abstract::Chenopods synthesize betaine by a two-step oxidation of choline: choline --> betaine aldehyde --> betaine. The pathway is chloroplastic; the first step has been shown in isolated spinach (Spinacia oleracea L.) chloroplasts to be O(2)- and light-dependent, the role of light being to provide reducing power (P Weigel, EA...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.88.3.695

    authors: Lerma C,Hanson AD,Rhodes D

    更新日期:1988-11-01 00:00:00

  • Enzymes of the Poly-beta-Hydroxybutyrate and Citric Acid Cycles of Rhizobium japonicum Bacteroids.

    abstract::The activities of several enzymes of the citric acid and poly-beta-hydroxybutyrate cycles were measured in Rhizobium japonicum 3I1B-143 bacteroids which had been isolated from soybean nodules by sucrose gradient centrifugation. During the period of developing nitrogenase activity, the specific activity of fumarase, hy...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.75.4.1158

    authors: Karr DB,Waters JK,Suzuki F,Emerich DW

    更新日期:1984-08-01 00:00:00

  • Non-targeted and targeted protein movement through plasmodesmata in leaves in different developmental and physiological states.

    abstract::Plant cells rely on plasmodesmata for intercellular transport of small signaling molecules as well as larger informational macromolecules such as proteins. A green fluorescent protein (GFP) reporter and low-pressure microprojectile bombardment were used to quantify the degree of symplastic continuity between cells of ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.125.4.1802

    authors: Crawford KM,Zambryski PC

    更新日期:2001-04-01 00:00:00

  • Plasmalemma redox activity in the diatom thalassiosira: a possible role for nitrate reductase.

    abstract::Plasmalemma redox activity in the diatom Thalassiosira is competitively inhibited by antiserum prepared against algal nitrate reductase (NR), and fluorescent labeling experiments reveal the binding of NR antiserum to the cell surface. Furthermore, the external electron acceptor Cu bathophenanthroline disulfonate cause...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.87.1.143

    authors: Jones GJ,Morel FM

    更新日期:1988-05-01 00:00:00

  • WRI1 is required for seed germination and seedling establishment.

    abstract::Storage compound accumulation during seed development prepares the next generation of plants for survival. Therefore, processes involved in the regulation and synthesis of storage compound accumulation during seed development bear relevance to germination and seedling establishment. The wrinkled1 (wri1) mutant of Arab...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.106.079574

    authors: Cernac A,Andre C,Hoffmann-Benning S,Benning C

    更新日期:2006-06-01 00:00:00

  • Peroxygenase-Catalyzed Fatty Acid Epoxidation in Cereal Seeds (Sequential Oxidation of Linoleic Acid into 9(S),12(S),13(S)-Trihydroxy-10(E)-Octadecenoic Acid).

    abstract::Peroxygenase-catalyzed epoxidation of oleic acid in preparations of cereal seeds was investigated. The 105,000g particle fraction of oat (Avena sativa) seed homogenate showed high peroxygenase activity, i.e. 3034 [plus or minus] 288 and 2441 [plus or minus] 168 nmol (10 min)-1 mg-1 protein in two cultivars, whereas th...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.110.3.807

    authors: Hamberg M,Hamberg G

    更新日期:1996-03-01 00:00:00

  • A stress-inducible resveratrol O-methyltransferase involved in the biosynthesis of pterostilbene in grapevine.

    abstract::Stilbenes are considered the most important phytoalexin group in grapevine (Vitis vinifera) and they are known to contribute to the protection against various pathogens. The main stilbenes in grapevine are resveratrol and its derivatives and, among these, pterostilbene has recently attracted much attention due both to...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.108.126003

    authors: Schmidlin L,Poutaraud A,Claudel P,Mestre P,Prado E,Santos-Rosa M,Wiedemann-Merdinoglu S,Karst F,Merdinoglu D,Hugueney P

    更新日期:2008-11-01 00:00:00

  • Molecular and biochemical characterization of 2-hydroxyisoflavanone dehydratase. Involvement of carboxylesterase-like proteins in leguminous isoflavone biosynthesis.

    abstract::Isoflavonoids are ecophysiologically active secondary metabolites of the Leguminosae and known for health-promoting phytoestrogenic functions. Isoflavones are synthesized by 1,2-elimination of water from 2-hydroxyisoflavanones, the first intermediate with the isoflavonoid skeleton, but details of this dehydration have...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.104.056747

    authors: Akashi T,Aoki T,Ayabe S

    更新日期:2005-03-01 00:00:00

  • Photosynthesis in Eurasian Watermilfoil (Myriophyllum spicatum L.).

    abstract::Gas exchange of Eurasian watermilfoil (Myriophyllum spicatum L.) indicated a near-zero CO(2) compensation point and a high temperature optimum for photosynthesis. These properties are characteristic of plants fixing CO(2) by a beta-carboxylation mechanism. Operation of the Calvin cycle was shown and no evidence for be...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.50.1.149

    authors: Stanley RA,Naylor AW

    更新日期:1972-07-01 00:00:00

  • Superoxide dismutase in Arabidopsis: an eclectic enzyme family with disparate regulation and protein localization.

    abstract::A number of environmental stresses can lead to enhanced production of superoxide within plant tissues, and plants are believed to rely on the enzyme superoxide dismutase (SOD) to detoxify this reactive oxygen species. We have identified seven cDNAs and genes for SOD in Arabidopsis. These consist of three CuZnSODs (CSD...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.118.2.637

    authors: Kliebenstein DJ,Monde RA,Last RL

    更新日期:1998-10-01 00:00:00

  • Modification of cytokinins by cauliflower microsomal enzymes.

    abstract::N(6)-(Delta(2)-isopentenyl)Adenine and N(6)-(Delta(2)-isopentenyl)adenosine were hydroxylated, respectively, to 6-(4-hydroxy-3-methyl-trans-2-butenylamino)purine and 6-(4-hydroxy-3-methyl-trans-2-butenylamino) -9-beta-ribofuranosylpurine in the presence of NADPH and the microsomal fraction from cauliflowers (Brassica ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.75.2.442

    authors: Chen CM,Leisner SM

    更新日期:1984-06-01 00:00:00

  • Accumulation of Free Proline in Citrus Leaves during Cold Hardening of Young Trees in Controlled Temperature Regimes.

    abstract::Free proline increased in leaves of orange (Citrus sinensis [L.] Osb. cv. Valencia) and grapefruit (Citrus paradisi Macfad. cv. Star Ruby) trees on a wide range of citrus rootstocks during cold hardening. Increases in sugars accompanied proline accumulation. During cold hardening, the rate of proline accumulation was ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.64.3.425

    authors: Yelenosky G

    更新日期:1979-09-01 00:00:00

  • Cytoplasmic free Ca2+ in Arabidopsis roots changes in response to touch but not gravity.

    abstract::Changes in cytoplasmic Ca2+ concentration ([Ca2+]i) have been proposed to be involved in signal transduction pathways in response to a number of stimuli, including gravity and touch. The current hypothesis proposes that the development of gravitropic bending is correlated with a redistribution of [Ca2+]i in gravistimu...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.114.3.789

    authors: Legué V,Blancaflor E,Wymer C,Perbal G,Fantin D,Gilroy S

    更新日期:1997-07-01 00:00:00

  • Inhibition of ethylene production by cobaltous ion.

    abstract::The effect of Co(2+) on ethylene production by mung bean (Phaseolus aureus Roxb.) and by apple tissues was studied. Co(2+), depending on concentrations applied, effectively inhibited ethylene production by both tissues. It also strongly inhibited the ethylene production induced by IAA, kinetin, IAA plus kinetin, Ca(2+...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.58.1.114

    authors: Lau OL,Yang SF

    更新日期:1976-07-01 00:00:00

  • Loss-of-function mutations of ROOT HAIR DEFECTIVE3 suppress root waving, skewing, and epidermal cell file rotation in Arabidopsis.

    abstract::Wild-type Arabidopsis (Arabidopsis thaliana L. Heynh.) roots growing on a tilted surface of impenetrable hard-agar media adopt a wave-like pattern and tend to skew to the right of the gravity vector (when viewed from the back of the plate through the medium). Reversible root-tip rotation often accompanies the clockwis...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.105.059774

    authors: Yuen CY,Sedbrook JC,Perrin RM,Carroll KL,Masson PH

    更新日期:2005-06-01 00:00:00

  • Evidence for osmotic regulation of hydrolytic enzyme production in germinating barley seeds.

    abstract::alpha-Amylase levels in intact seeds of barley (Hordeum vulgare L. cv. Himalaya) reach a maximum at 3 to 4 days of germination while gibberellin levels continue to increase beyond 6 days of germination. In contrast to its effect on half seeds, gibberellic acid does not increase the total amount of alpha-amylase produc...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.48.2.137

    authors: Jones RL,Armstrong JE

    更新日期:1971-08-01 00:00:00

  • Sodium Requirement for Photosynthesis and Its Relationship with Dinitrogen Fixation and the External CO(2) Concentration in Cyanobacteria.

    abstract::Cells of Anabaena PCC 7119 and of a mutant strain of Nostoc muscorum unable to fix dinitrogen, grown at pH 8 and under low CO(2) tension (air), showed a reduced capacity for photosynthesis when cultured in the absence of sodium, this inhibition being followed by symptoms of photooxidation, such as chlorosis, oxygen co...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.85.2.585

    authors: Maeso ES,Piñas FF,Gonzalez MG,Valiente EF

    更新日期:1987-10-01 00:00:00

  • Targets of the StBEL5 Transcription Factor Include the FT Ortholog StSP6A.

    abstract::The BEL1-like family of transcription factors is ubiquitous in plants and plays important roles in regulating development. They function in tandem with KNOTTED1 types to bind to a double TTGAC motif in the upstream sequence of target genes. StBEL5 of potato (Solanum tuberosum) functions as a mobile RNA signal that is ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.15.01314

    authors: Sharma P,Lin T,Hannapel DJ

    更新日期:2016-01-01 00:00:00

  • A simple theory regarding ambimobility of xenobiotics with special reference to the nematicide, oxamyl.

    abstract::A theory is presented to explain the phloem mobility of certain systemic xenobiotics that are not weak acids. It is shown that there is a theoretically optimum permeability that permits optimum circulation through the symplasm and apoplast (including the phloem and xylem) of Solanum tuberosum plants. The optimum perme...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.63.2.367

    authors: Tyree MT

    更新日期:1979-02-01 00:00:00

  • mRNA Localization in Plant Cells.

    abstract::Localization of mRNAs at the subcellular level is an essential mechanism for specific protein targeting and local control of protein synthesis in both eukaryotes and bacteria. While mRNA localization is well documented in metazoans, somatic cells, and microorganisms, only a handful of well-defined mRNA localization ex...

    journal_title:Plant physiology

    pub_type: 杂志文章,评审

    doi:10.1104/pp.19.00972

    authors: Tian L,Chou HL,Fukuda M,Kumamaru T,Okita TW

    更新日期:2020-01-01 00:00:00

  • Effect of glucose on the induction of nitrate reductase in corn roots.

    abstract::In Zea mays L., addition of glucose to the induction medium has no effect on the induction of nitrate reductase during the initial 3 hours either in root tips (0-10 mm) or mature root sections (25-35 mm). With longer times, higher levels of enzyme activity are recovered from both root segments when glucose is present ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.56.5.634

    authors: Aslam M,Oaks A

    更新日期:1975-11-01 00:00:00

  • The Liverwort, Marchantia, Drives Alternative Electron Flow Using a Flavodiiron Protein to Protect PSI.

    abstract::The diffusion efficiency of oxygen in the atmosphere, like that of CO2, is approximately 104 times greater than that in aqueous environments. Consequently, terrestrial photosynthetic organisms need mechanisms to protect against potential oxidative damage. The liverwort Marchantia polymorpha, a basal land plant, has ha...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.16.01038

    authors: Shimakawa G,Ishizaki K,Tsukamoto S,Tanaka M,Sejima T,Miyake C

    更新日期:2017-03-01 00:00:00