Abstract:
:Rabbit antiserum against beta-amylase isolated from germinating seeds of rice was produced, and its specific cross-reactivity with beta-amylase was confirmed by means of Ouchterlony double immunodiffusion and immunoelectrophoresis procedures. The cellular localization of beta-amylase was studied by indirect fluorescence microscopy of thin sectioned germinating rice seed specimens (1-day stage) which had been fixed and treated with purified rabbit anti-beta-amylase immunoglobulin G followed by conjugation with fluorescein isothiocyanate-labeled goat antirabbit immunoglobulin G. It has been demonstrated that beta-amylase is uniformly associated with the periphery of starch granules in the starchy endosperm cells. The finding is discussed in relation to the general notion concerning the presence of the latent form of beta-amylase bound to protein bodies in cereal seeds.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Okamoto K,Akazawa Tdoi
10.1104/pp.64.2.337subject
Has Abstractpub_date
1979-08-01 00:00:00pages
337-40issue
2eissn
0032-0889issn
1532-2548journal_volume
64pub_type
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