Functional Promiscuity of Two Divergent Paralogs of Type III Plant Polyketide Synthases.

Abstract:

:Plants effectively defend themselves against biotic and abiotic stresses by synthesizing diverse secondary metabolites, including health-protective flavonoids. These display incredible chemical diversity and ubiquitous occurrence and confer impeccable biological and agricultural applications. Chalcone synthase (CHS), a type III plant polyketide synthase, is critical for flavonoid biosynthesis. It catalyzes acyl-coenzyme A thioesters to synthesize naringenin chalcone through a polyketidic intermediate. The functional divergence among the evolutionarily generated members of a gene family is pivotal in driving the chemical diversity. Against this backdrop, this study was aimed to functionally characterize members of the CHS gene family from Rheum emodi, an endangered and endemic high-altitude medicinal herb of northwestern Himalayas. Two full-length cDNAs (1,179 bp each), ReCHS1 and ReCHS2, encoding unique paralogs were isolated and characterized. Heterologous expression and purification in Escherichia coli, bottom-up proteomic characterization, high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry analysis, and enzyme kinetic studies using five different substrates confirmed their catalytic potential. Phylogenetic analysis revealed the existence of higher synonymous mutations in the intronless divergents of ReCHS. ReCHS2 displayed significant enzymatic efficiency (Vmax/Km) with different substrates. There were significant spatial and altitudinal variations in messenger RNA transcript levels of ReCHSs correlating positively with metabolite accumulation. Furthermore, the elicitations in the form of methyl jasmonate, salicylic acid, ultraviolet B light, and wounding, chosen on the basis of identified cis-regulatory promoter elements, presented considerable differences in the transcript profiles of ReCHSs. Taken together, our results demonstrate differential propensities of CHS paralogs in terms of the accumulation of flavonoids and their relative substrate selectivities.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Pandith SA,Dhar N,Rana S,Bhat WW,Kushwaha M,Gupta AP,Shah MA,Vishwakarma R,Lattoo SK

doi

10.1104/pp.16.00003

subject

Has Abstract

pub_date

2016-08-01 00:00:00

pages

2599-619

issue

4

eissn

0032-0889

issn

1532-2548

pii

pp.16.00003

journal_volume

171

pub_type

杂志文章
  • Molecular characterization of oat seed globulins.

    abstract::We have isolated full-length cDNA clones that encode oat (Avena sativa) seed storage globulin mRNAs from a cDNA library in the expression vector lambda gtll. The longest of these clones, pOG2, has an 1840-base pair insert that encodes a complete precursor subunit with a signal peptide of 24 amino acids followed by an ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.87.3.698

    authors: Shotwell MA,Afonso C,Davies E,Chesnut RS,Larkins BA

    更新日期:1988-07-01 00:00:00

  • Resemblance and dissemblance of Arabidopsis type II peroxiredoxins: similar sequences for divergent gene expression, protein localization, and activity.

    abstract::The Arabidopsis type II peroxiredoxin (PRXII) family is composed of six different genes, five of which are expressed. On the basis of the nucleotide and protein sequences, we were able to define three subgroups among the PRXII family. The first subgroup is composed of AtPRXII-B, -C, and -D, which are highly similar an...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.103.022533

    authors: Bréhélin C,Meyer EH,de Souris JP,Bonnard G,Meyer Y

    更新日期:2003-08-01 00:00:00

  • Overexpression of an isoprenyl diphosphate synthase in spruce leads to unexpected terpene diversion products that function in plant defense.

    abstract::Spruce (Picea spp.) and other conifers employ terpenoid-based oleoresin as part of their defense against herbivores and pathogens. The short-chain isoprenyl diphosphate synthases (IDS) are situated at critical branch points in terpene biosynthesis, producing the precursors of the different terpenoid classes. To determ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.113.228940

    authors: Nagel R,Berasategui A,Paetz C,Gershenzon J,Schmidt A

    更新日期:2014-02-01 00:00:00

  • Inhibition of adenosine triphosphatase activity of the plasma membrane fraction of oat roots by diethylstilbestrol.

    abstract::Diethylstibestrol (DES) inhibited noncompetitively the ATPase in the plasma membrane fraction from Avena sativa L. cv. Goodfield roots when assayed in the presence of MgSO(4) or MgSO(4) plus KCl. In the presence of MgSO(4), 7.1x10(-5) molar DES inhibited the enzyme 50%; whereas in the presence of MgSO(4) and KCl, 1.3x...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.63.1.48

    authors: Balke NE,Hodges TK

    更新日期:1979-01-01 00:00:00

  • Effects of temperature on orthophosphate absorption by excised corn roots.

    abstract::The uptake of orthophosphate ((32)P) by excised corn roots, Zea mays L. was studied using roots grown on 0.2 mm CaSO(4). Nine concentrations of KH(2)PO(4) from 1 to 256 mum were used at temperatures of 20 degrees , 30 degrees , and 40 degrees . Enzyme kinetic analysis was applied to the data obtained. Two apparent mec...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.42.10.1407

    authors: Carter OG,Lathwell DJ

    更新日期:1967-10-01 00:00:00

  • AtFXG1, an Arabidopsis gene encoding alpha-L-fucosidase active against fucosylated xyloglucan oligosaccharides.

    abstract::An alpha-L-fucosidase (EC 3.2.1.51) able to release the t-fucosyl residue from the side chain of xyloglucan oligosaccharides has been detected in the leaves of Arabidopsis plants. Moreover, an alpha-L-fucosidase with similar substrate specificity was purified from cabbage (Brassica oleracea) leaves to render a single ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:

    authors: de La Torre F,Sampedro J,Zarra I,Revilla G

    更新日期:2002-01-01 00:00:00

  • l-Ornithine:2-Oxoacid Aminotransferase from Squash (Cucurbita pepo, L.) Cotyledons: Purification and Properties.

    abstract::ORNITHINE: 2-oxoacid aminotransferase (EC 2.6.1.13) has been purified over 400-fold with a total recovery of 14% from acetone powders of cotyledons of germinating squash (Cucurbita pepo, L.) seedlings. The pH optimum of the transamination between l-ornithine and alpha-ketoglutarate is 8 and the Michaelis constants are...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.55.3.502

    authors: Lu TS,Mazelis M

    更新日期:1975-03-01 00:00:00

  • Effects of sulfur nutrition on expression of the soybean seed storage protein genes in transgenic petunia.

    abstract::The 7S seed storage protein (beta-conglycinin) of soybean (Glycine max [L]. Merr.) has three major subunits; alpha, alpha', and beta. Accumulation of the beta-subunit, but not the alpha- and alpha'-subunits, has been shown to be repressed by exogenously applied methionine to the immature cotyledon culture system (LP H...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.99.1.263

    authors: Fujiwara T,Hirai MY,Chino M,Komeda Y,Naito S

    更新日期:1992-05-01 00:00:00

  • The C-Domain of Oleuropein β-Glucosidase Assists in Protein Folding and Sequesters the Enzyme in Nucleus.

    abstract::Oleuropein, a terpene-derived glycosylated secoiridoid biosynthesized exclusively by members of the Oleaceae family, is involved in a two-component defense system comprising a β-glucosidase that activates oleuropein into a toxic glutaraldehyde-like structure. Oleuropein and its deglycosylated derivatives have high pha...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.17.00512

    authors: Koudounas K,Thomopoulou M,Michaelidis C,Zevgiti E,Papakostas G,Tserou P,Daras G,Hatzopoulos P

    更新日期:2017-07-01 00:00:00

  • Transcriptional profiling of the Arabidopsis embryo.

    abstract::We have used laser-capture microdissection to isolate RNA from discrete tissues of globular, heart, and torpedo stage embryos of Arabidopsis (Arabidopsis thaliana). This was amplified and analyzed by DNA microarray using the Affymetrix ATH1 GeneChip, representing approximately 22,800 Arabidopsis genes. Cluster analysi...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.106.087668

    authors: Spencer MW,Casson SA,Lindsey K

    更新日期:2007-02-01 00:00:00

  • Contribution of Nonautotrophic Carbon Dioxide Fixation to Protein Synthesis in Suspension Cultures of Paul's Scarlet Rose.

    abstract::Bicarbonate-(14)C was provided to 5- and 11-day-old suspension cultures of Paul's Scarlet rose, and the incorporation of (14)C into lipid, protein, amino acids, and organic acids was determined. The rate of bicarbonate uptake was approximately the same by 5- and 11-day-old cells, but the distribution of (14)C among ce...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.55.4.643

    authors: Nesius KK,Fletcher JS

    更新日期:1975-04-01 00:00:00

  • The Multisite PeachRefPop Collection: A True Cultural Heritage and International Scientific Tool for Fruit Trees.

    abstract::Plants have evolved a range of adaptive mechanisms that adjust their development and physiology to variable external conditions, particularly in perennial species subjected to long-term interplay with the environment. Exploiting the allelic diversity within available germplasm and leveraging the knowledge of the mecha...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.19.01412

    authors: Cirilli M,Micali S,Aranzana MJ,Arús P,Babini A,Barreneche T,Bink M,Cantin CM,Ciacciulli A,Cos-Terrer JE,Drogoudi P,Eduardo I,Foschi S,Giovannini D,Guerra W,Liverani A,Pacheco I,Pascal T,Quilot-Turion B,Verde I,Ros

    更新日期:2020-10-01 00:00:00

  • A Short Open Reading Frame Encompassing the MicroRNA173 Target Site Plays a Role in trans-Acting Small Interfering RNA Biogenesis.

    abstract::trans-Acting small interfering RNAs (tasiRNAs) participate in the regulation of organ morphogenesis and determination of developmental timing in plants by down-regulating target genes through mRNA cleavage. The production of tasiRNAs is triggered by microRNA173 (miR173) and other specific microRNA-mediated cleavage of...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.16.00148

    authors: Yoshikawa M,Iki T,Numa H,Miyashita K,Meshi T,Ishikawa M

    更新日期:2016-05-01 00:00:00

  • A shift toward smaller cell size via manipulation of cell cycle gene expression acts to smoothen Arabidopsis leaf shape.

    abstract::Understanding the relationship of the size and shape of an organism to the size, shape, and number of its constituent cells is a basic problem in biology; however, numerous studies indicate that the relationship is complex and often nonintuitive. To investigate this problem, we used a system for the inducible expressi...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.111.176073

    authors: Kuwabara A,Backhaus A,Malinowski R,Bauch M,Hunt L,Nagata T,Monk N,Sanguinetti G,Fleming A

    更新日期:2011-08-01 00:00:00

  • Architecture-based multiscale computational modeling of plant cell wall mechanics to examine the hydrogen-bonding hypothesis of the cell wall network structure model.

    abstract::A primary plant cell wall network was computationally modeled using the finite element approach to study the hypothesis of hemicellulose (HC) tethering with the cellulose microfibrils (CMFs) as one of the major load-bearing mechanisms of the growing cell wall. A computational primary cell wall network fragment (10 × 1...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.112.201228

    authors: Yi H,Puri VM

    更新日期:2012-11-01 00:00:00

  • Genetic enhancement of fatty acid synthesis by targeting rat liver ATP:citrate lyase into plastids of tobacco.

    abstract::ATP:citrate lyase (ACL) catalyzes the conversion of citrate to acetyl-coenzyme A (CoA) and oxaloacetate and is a key enzyme for lipid accumulation in mammals and oleaginous yeasts and fungi. To investigate whether heterologous ACL genes can be targeted and imported into the plastids of plants, a gene encoding a fusion...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.122.4.1231

    authors: Rangasamy D,Ratledge C

    更新日期:2000-04-01 00:00:00

  • A new resource for cereal genomics: 22K barley GeneChip comes of age.

    abstract::In recent years, access to complete genomic sequences, coupled with rapidly accumulating data related to RNA and protein expression patterns, has made it possible to determine comprehensively how genes contribute to complex phenotypes. However, for major crop plants, publicly available, standard platforms for parallel...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.103.034462

    authors: Close TJ,Wanamaker SI,Caldo RA,Turner SM,Ashlock DA,Dickerson JA,Wing RA,Muehlbauer GJ,Kleinhofs A,Wise RP

    更新日期:2004-03-01 00:00:00

  • Pathway-level acceleration of glycogen catabolism by a response regulator in the cyanobacterium Synechocystis species PCC 6803.

    abstract::Response regulators of two-component systems play pivotal roles in the transcriptional regulation of responses to environmental signals in bacteria. Rre37, an OmpR-type response regulator, is induced by nitrogen depletion in the unicellular cyanobacterium Synechocystis species PCC 6803. Microarray and quantitative rea...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.113.232025

    authors: Osanai T,Oikawa A,Numata K,Kuwahara A,Iijima H,Doi Y,Saito K,Hirai MY

    更新日期:2014-04-01 00:00:00

  • Male Sterility in Maize after Transient Heat Stress during the Tetrad Stage of Pollen Development.

    abstract::Shifts in the duration and intensity of ambient temperature impair plant development and reproduction, particularly male gametogenesis. Stress exposure causes meiotic defects or premature spore abortion in male reproductive organs, leading to male sterility. However, little is known about the mechanisms underlying str...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.19.00707

    authors: Begcy K,Nosenko T,Zhou LZ,Fragner L,Weckwerth W,Dresselhaus T

    更新日期:2019-10-01 00:00:00

  • Distinct cell wall architectures in seed endosperms in representatives of the Brassicaceae and Solanaceae.

    abstract::In some species, a crucial role has been demonstrated for the seed endosperm during germination. The endosperm has been shown to integrate environmental cues with hormonal networks that underpin dormancy and seed germination, a process that involves the action of cell wall remodeling enzymes (CWREs). Here, we examine ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.112.203661

    authors: Lee KJ,Dekkers BJ,Steinbrecher T,Walsh CT,Bacic A,Bentsink L,Leubner-Metzger G,Knox JP

    更新日期:2012-11-01 00:00:00

  • Comparison of the Ability of Salicylic Acid and Ferricyanide to Induce Flowering in the Long-day Plant, Lemna Gibba G3.

    abstract::Both salicylic acid and ferricyanide induce flowering in the long-day plant Lemna gibba L., strain G3 under 8- and 9-hour short days. In both cases the effect is daylength-dependent. Salicylic acid is ineffective on daylengths less than 8 hours and ferricyanide is ineffective on daylengths less than 5 hours. When both...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.65.6.1058

    authors: Tanaka O,Cleland CF

    更新日期:1980-06-01 00:00:00

  • Buoyant density studies of chloroplast and nuclear deoxyribonucleic acid from control and 3-amino-1,2,4-triazole-treated wheat seedlings, Triticum vulgare.

    abstract::The isolation of chloroplast and nuclear DNA from dark- and light-grown, control- and 3-amino-1,2,4-triazole-treated wheat seedlings, Triticum vulgare, is described. Contrary to a previous report, we found that chloroplast and nuclear DNA had similar buoyant densities (1.702 grams per cubic centimeter) and that they c...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.46.6.825

    authors: Bartels PG,Hyde A

    更新日期:1970-12-01 00:00:00

  • Altered gene expression in three plant species in response to treatment with Nep1, a fungal protein that causes necrosis.

    abstract::Nep1 is an extracellular fungal protein that causes necrosis when applied to many dicotyledonous plants, including invasive weed species. Using transmission electron microscopy, it was determined that application of Nep1 (1.0 micro g mL(-)(1), 0.1% [v/v] Silwet-L77) to Arabidopsis and two invasive weed species, spotte...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.102.019836

    authors: Keates SE,Kostman TA,Anderson JD,Bailey BA

    更新日期:2003-07-01 00:00:00

  • The requirement for sodium as a micronutrient by species having the c(4) dicarboxylic photosynthetic pathway.

    abstract::Six species having characteristics of plants with the C(4) dicarboxylic photosynthetic pathway, Echinochloa utilis L. Ohwi et Yabuno (Japanese millet), Cynodon dactylon L. (Bermuda grass), Kyllinga brevifolia Rottb., Amaranthus tricolor L. cv. Early splendour, Kochia childsii Hort., and Portulaca grandiflora Hook (ros...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.49.5.794

    authors: Brownell PF,Crossland CJ

    更新日期:1972-05-01 00:00:00

  • Adenine phosphoribosyltransferase in plant tissues: some effects of kinetin on enzymic activity.

    abstract::Adenine phosphoribosyltransferase activity was measured in extracts of soybean (Glycine max var. Acme) callus and of senescing barley leaves (Hordeum distichon c.v. Prior). The enzyme from soybean callus had Michaelis constants for adenine and 5-phosphoribosyl pyrophosphate of 1.5 and 7.5 mum respectively and was inhi...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.43.4.645

    authors: Nicholls PB,Murray AW

    更新日期:1968-04-01 00:00:00

  • A cyclic nucleotide-gated channel (CNGC16) in pollen is critical for stress tolerance in pollen reproductive development.

    abstract::Cyclic nucleotide-gated channels (CNGCs) have been implicated in diverse aspects of plant growth and development, including responses to biotic and abiotic stress, as well as pollen tube growth and fertility. Here, genetic evidence identifies CNGC16 in Arabidopsis (Arabidopsis thaliana) as critical for pollen fertilit...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.112.206888

    authors: Tunc-Ozdemir M,Tang C,Ishka MR,Brown E,Groves NR,Myers CT,Rato C,Poulsen LR,McDowell S,Miller G,Mittler R,Harper JF

    更新日期:2013-02-01 00:00:00

  • Comparative freezing patterns in stems of cherry and azalea.

    abstract::Ice formation in stems, as determined by means of an electrophoretic mobility technique, occurs much more rapidly in azalea than in sour cherry. The difference is more marked in the bark than in the wood. Disrupting the structure of the tissues completely eliminates differences in freezing patterns, although gross ana...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.50.5.527

    authors: Dennis FG,Lumis GP,Olien CR

    更新日期:1972-11-01 00:00:00

  • Regulation of Glutamine Synthetase by Light and during Nitrogen Deficiency in Synchronous Chlorella sorokiniana.

    abstract::A method is described to achieve density labeling of proteins in unicellular algae by using (13)CO(2). This is a satisfactory procedure especially for work on nitrogen metabolism. The increase in activity of glutamine synthetase (EC 6.3.1.2.) and glutamate synthase (EC 1.4.7.1.) in Chlorella sorokiniana mediated by a ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.66.5.805

    authors: Tischner R

    更新日期:1980-11-01 00:00:00

  • The NAC Transcription Factors OsNAC20 and OsNAC26 Regulate Starch and Storage Protein Synthesis.

    abstract::Starch and storage proteins determine the weight and quality of cereal grains. Synthesis of these two grain components has been comprehensively investigated, but the transcription factors responsible for their regulation remain largely unknown. In this study, we investigated the roles of NAM, ATAF, and CUC (NAC) trans...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.20.00984

    authors: Wang J,Chen Z,Zhang Q,Meng S,Wei C

    更新日期:2020-12-01 00:00:00

  • Selective enhancement of alternative path capacity in plant storage organs in response to ethylene plus oxygen: a comparative study.

    abstract::The respiration rise in bulky storage organs induced by ethylene plus pure O(2) is accompanied by an increase in the CN-resistant respiration, or alternative path. Whereas a lesser respiratory stimulation in response to ethylene is demonstrable in air and increased by peeling, ethylene-induced alternative path develop...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.69.5.1036

    authors: Theologis A,Laties GG

    更新日期:1982-05-01 00:00:00