Abstract:
:The isolation of chloroplast and nuclear DNA from dark- and light-grown, control- and 3-amino-1,2,4-triazole-treated wheat seedlings, Triticum vulgare, is described. Contrary to a previous report, we found that chloroplast and nuclear DNA had similar buoyant densities (1.702 grams per cubic centimeter) and that they could not be resolved by buoyant density centrifugation in CsCl. Difference in renaturation behavior of the chloroplast and nuclear DNA was used as the criterion for distinguishing one from the other. Only chloroplast DNA readily renatured whereas nuclear DNA renatured only slightly. Light-grown, 3-amino-1,2,4-triazole-treated plants were found to lack detectable quantities of chloroplast DNA whereas treated, dark-grown plants contained plastid DNA. We suggest that 3-amino-1,2,4-triazole affects the accumulation of chloroplast DNA by inhibiting the formation of chloroplast membranes, enzymes, and pigments.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Bartels PG,Hyde Adoi
10.1104/pp.46.6.825subject
Has Abstractpub_date
1970-12-01 00:00:00pages
825-30issue
6eissn
0032-0889issn
1532-2548journal_volume
46pub_type
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