Transcriptional dynamics of two seed compartments with opposing roles in Arabidopsis seed germination.

Abstract:

:Seed germination is a critical stage in the plant life cycle and the first step toward successful plant establishment. Therefore, understanding germination is of important ecological and agronomical relevance. Previous research revealed that different seed compartments (testa, endosperm, and embryo) control germination, but little is known about the underlying spatial and temporal transcriptome changes that lead to seed germination. We analyzed genome-wide expression in germinating Arabidopsis (Arabidopsis thaliana) seeds with both temporal and spatial detail and provide Web-accessible visualizations of the data reported (vseed.nottingham.ac.uk). We show the potential of this high-resolution data set for the construction of meaningful coexpression networks, which provide insight into the genetic control of germination. The data set reveals two transcriptional phases during germination that are separated by testa rupture. The first phase is marked by large transcriptome changes as the seed switches from a dry, quiescent state to a hydrated and active state. At the end of this first transcriptional phase, the number of differentially expressed genes between consecutive time points drops. This increases again at testa rupture, the start of the second transcriptional phase. Transcriptome data indicate a role for mechano-induced signaling at this stage and subsequently highlight the fates of the endosperm and radicle: senescence and growth, respectively. Finally, using a phylotranscriptomic approach, we show that expression levels of evolutionarily young genes drop during the first transcriptional phase and increase during the second phase. Evolutionarily old genes show an opposite pattern, suggesting a more conserved transcriptome prior to the completion of germination.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Dekkers BJ,Pearce S,van Bolderen-Veldkamp RP,Marshall A,Widera P,Gilbert J,Drost HG,Bassel GW,Müller K,King JR,Wood AT,Grosse I,Quint M,Krasnogor N,Leubner-Metzger G,Holdsworth MJ,Bentsink L

doi

10.1104/pp.113.223511

subject

Has Abstract

pub_date

2013-09-01 00:00:00

pages

205-15

issue

1

eissn

0032-0889

issn

1532-2548

pii

pp.113.223511

journal_volume

163

pub_type

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