Abstract:
:Seed germination is a critical stage in the plant life cycle and the first step toward successful plant establishment. Therefore, understanding germination is of important ecological and agronomical relevance. Previous research revealed that different seed compartments (testa, endosperm, and embryo) control germination, but little is known about the underlying spatial and temporal transcriptome changes that lead to seed germination. We analyzed genome-wide expression in germinating Arabidopsis (Arabidopsis thaliana) seeds with both temporal and spatial detail and provide Web-accessible visualizations of the data reported (vseed.nottingham.ac.uk). We show the potential of this high-resolution data set for the construction of meaningful coexpression networks, which provide insight into the genetic control of germination. The data set reveals two transcriptional phases during germination that are separated by testa rupture. The first phase is marked by large transcriptome changes as the seed switches from a dry, quiescent state to a hydrated and active state. At the end of this first transcriptional phase, the number of differentially expressed genes between consecutive time points drops. This increases again at testa rupture, the start of the second transcriptional phase. Transcriptome data indicate a role for mechano-induced signaling at this stage and subsequently highlight the fates of the endosperm and radicle: senescence and growth, respectively. Finally, using a phylotranscriptomic approach, we show that expression levels of evolutionarily young genes drop during the first transcriptional phase and increase during the second phase. Evolutionarily old genes show an opposite pattern, suggesting a more conserved transcriptome prior to the completion of germination.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Dekkers BJ,Pearce S,van Bolderen-Veldkamp RP,Marshall A,Widera P,Gilbert J,Drost HG,Bassel GW,Müller K,King JR,Wood AT,Grosse I,Quint M,Krasnogor N,Leubner-Metzger G,Holdsworth MJ,Bentsink Ldoi
10.1104/pp.113.223511subject
Has Abstractpub_date
2013-09-01 00:00:00pages
205-15issue
1eissn
0032-0889issn
1532-2548pii
pp.113.223511journal_volume
163pub_type
杂志文章相关文献
PLANT PHYSIOLOGY文献大全abstract::We examined the response of primary roots of maize (Zea mays L. cv Merit) to unilateral application of calcium with particular attention to the site of application, the dependence on growth rate, and possible contributions of thigmotropic stimulation during application. Unilateral application of agar to the root cap ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.100.2.762
更新日期:1992-01-01 00:00:00
abstract::Cold acclimation and freezing tolerance are the result of complex interaction between low temperature, light, and photosystem II (PSII) excitation pressure. Previous results have shown that expression of the Wcs19 gene is correlated with PSII excitation pressure measured in vivo as the relative reduction state of PSII...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.001925
更新日期:2002-07-01 00:00:00
abstract::Ethylene inhibited the tuberization of etiolated potato (Solanum tuberosum L. var. Red La Soda) sprout sections cultured in vitro. Carbon dioxide did not overcome the C(2)H(4) inhibition but it was required for normal tuberization. Ethylene totally prevented root formation and development. It inhibited stolon elongati...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.57.4.480
更新日期:1976-04-01 00:00:00
abstract::Combined gas chromatography-mass spectrometry has been used to identify indole-3-methanol in a purified buffer extract from etiolated seedlings of Pinus sylvestris L. Quantitative estimates obtained by high performance liquid chromatography with fluorescence detection, corrected for losses occurring during purificatio...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.77.4.952
更新日期:1985-04-01 00:00:00
abstract::For an efficient defense response against pathogens, plants must coordinate rapid genetic reprogramming to produce an incompatible interaction. Nitrate Trasnporter2 (NRT2) gene family members are sentinels of nitrate availability. In this study, we present an additional role for NRT2.1 linked to plant resistance again...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.111.184424
更新日期:2012-02-01 00:00:00
abstract::Indole-3-acetic acid was applied asymmetrically to the hypocotyls of sunflower (Helianthus annuus L.) seedlings. After 5 hours on a clinostat, auxin gradients as small as 1 to 1.3 produced substantial (more than 60 degrees) hypocotyl curvature. This result suggests the asymmetric growth underlying hypocotyl gravitro...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.91.2.466
更新日期:1989-01-01 00:00:00
abstract::Electron transport activity at the cell surface of intact oat seedlings (Avena sativa L. cv Garry) was examined by measuring the oxidation and/or reduction of agents in the medium bathing the roots. Oxidation of NADH with or without added electron acceptors and reduction of ferricyanide by an endogenous electron donor...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.76.2.386
更新日期:1984-10-01 00:00:00
abstract::We present here a vector system to obtain homozygous marker-free transgenic plants without the need of extra handling and within the same time frame as compared to transformation methods in which the marker is not removed. By introducing a germline-specific auto-excision vector containing a cre recombinase gene under ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.107.106526
更新日期:2007-12-01 00:00:00
abstract::Thermal conductivity coefficients have been determined for longitudinal and transverse flow in 4 varieties of fresh Citrus wood using steady state-methods. Equations were developed from which thermal conductivity could be rapidly estimated from moisture content or electrical conductivity. The heat balance of large and...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.42.8.1025
更新日期:1967-08-01 00:00:00
abstract::In plants, the excess of several heavy metals mimics iron (Fe) deficiency-induced chlorosis, indicating a disturbance in Fe homeostasis. To examine the level at which heavy metals interfere with Fe deficiency responses, we carried out an in-depth characterization of Fe-related physiological, regulatory, and morphologi...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.16.01916
更新日期:2017-07-01 00:00:00
abstract::Malformin and ethylene stimulate abscission of the primary leaves of Phaseolus aureus Roxb. in the dark, and abscission stimulation by both compounds is inhibited by indeleacetic acid and CO(2). Ethylene production by malformin-treated buds is stimulated within 4 hours. and up to 8 days, after treatment. Malformin-ind...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.43.1.76
更新日期:1968-01-01 00:00:00
abstract::Pyridine nucleotide pools were measured in intact plastids from roots of barley (Hordeum vulgare L.) during the onset of NO2- assimilation and compared with the in vitro effect of the NADPH/NADP ratio on the activity of plastidic glucose-6-phosphate dehydrogenase (G6PDH, EC 1.1.1.49) from N-sufficient or N-starved roo...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.114.4.1413
更新日期:1997-08-01 00:00:00
abstract::Photosynthetic fixation of (14)CO(2) in the bean Phaseolus vulgaris, cv. Pencil Pod Black Wax, resulted in the appearance of labeled compounds that were characterized as phosphoserine and phosphohydroxypyruvate by chromatographic separation and by the synthesis of chemical derivatives. In (14)CO(2)/(12)CO(2) pulse-cha...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.60.1.109
更新日期:1977-07-01 00:00:00
abstract::The infectivity of Agrobacterium tumefaciens strain B6 was inhibited about 50% when these bacteria were inoculated on potato discs with equal viable cell counts of a weakly virulent strain of A. tumefaciens (B-48) or autoclaved strains of B6 or B-48. Inhibition by B-48 or autoclaved B6 could still be obtained when the...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.61.6.1031
更新日期:1978-06-01 00:00:00
abstract::The O(2) concentration in intact and osmotically disrupted isolated spinach (Spinacia oleracea, L.) chloroplasts during photosynthesis was estimated. The chloroplasts were allowed to reduce 3-phosphoglycerate, CO(2), or ferricyanide in light until the rate of O(2) production was linear. When the light was turned off O...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.60.6.903
更新日期:1977-12-01 00:00:00
abstract::A method has been developed to measure concentrations of CO(2) in gases rapidly. A gas sample is injected into a flowing carrier gas that passes through an infrared CO(2) analyzer. A strip chart recorded peak response is obtained which is proportional to the CO(2) concentration. A resolution of better than 2 microlite...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.62.6.924
更新日期:1978-12-01 00:00:00
abstract::We examined the response of leaves of 3-week-old maize (Zea mays L.) to short-term (5 h) fumigation with O3-enriched air (0, 0.12, 0.24, or 0.36 [mu]L/L). Older leaves and leaf tissue developed more severe visible damage at higher external O3 concentrations. To investigate the immediate effect of O3 exposure on the ac...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.108.2.777
更新日期:1995-06-01 00:00:00
abstract::Plants undergoing heat and low-CO2 stresses emit large amounts of volatile isoprenoids compared with those in stress-free conditions. One hypothesis posits that the balance between reducing power availability and its use in carbon assimilation determines constitutive isoprenoid emission rates in plants and potentially...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.114.246207
更新日期:2014-10-01 00:00:00
abstract::Seedlings of maize (Zea mays L. cv Pioneer 3906), hydroponically grown in the dark, were exposed to NaCl either gradually (salt acclimation) or in one step (salt shock). In the salt-acclimation treatment, root extension was indistinguishable from that of unsalinized controls for at least 6 d at concentrations up to 10...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.113.3.881
更新日期:1997-03-01 00:00:00
abstract::Changes in acyl lipids and pigments during leaf development in a virescens barley mutant (M) and the normal (N) were studied. Apical 3-cm leaf segments were extracted with chloroform-methanol, the extracts were purified on Sephadex G-25 columns, and the polar lipids were separated on two-dimensional-thin layer chromat...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.67.4.646
更新日期:1981-04-01 00:00:00
abstract::Waxy wheat (Triticum aestivum L.) lacks the waxy protein, which is also known as granule-bound starch synthase I (GBSSI). The starch granules of waxy wheat endosperm and pollen do not contain amylose and therefore stain red-brown with iodine. However, we observed that starch from pericarp tissue of waxy wheat stained ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.118.2.451
更新日期:1998-10-01 00:00:00
abstract::Rapidly dried Tortula ruralis, a drought-tolerant moss, is known to synthesize proteins on rehydration at a much lower rate than the slowly dried moss. The reasons for this low rate of protein synthesis are unclear. We have found that during rehydration of rapidly dried moss, there is a negative correlation between th...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.85.4.1094
更新日期:1987-12-01 00:00:00
abstract::Plant viral suppressors of RNA silencing induce developmental defects similar to those caused by mutations in genes involved in the microRNA pathway. A recent report has attributed viral suppressor-mediated developmental defects to up-regulation of AUXIN RESPONSE FACTOR 8 (ARF8), a target of miR167. The key piece of e...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.16.01030
更新日期:2016-11-01 00:00:00
abstract::Xyloglucan is an important hemicellulosic polysaccharide in dicot primary cell walls. Most of the enzymes involved in xyloglucan synthesis have been identified. However, many important details of its synthesis in vivo remain unknown. The roles of three genes encoding xylosyltransferases participating in xyloglucan bio...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.112.198119
更新日期:2012-08-01 00:00:00
abstract::Persistent organic pollutants, such as polychlorinated biphenyls (PCBs), are a global problem. We demonstrate enhanced depletion of PCBs using root-associated microbes, which can use plant secondary metabolites, such as phenylpropanoids. Using a "rhizosphere metabolomics" approach, we show that phenylpropanoids consti...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.102.016295
更新日期:2003-05-01 00:00:00
abstract::Relative elemental growth rate (REGR) profiles describe spatial patterns of growth intensity; they are indispensable for causal growth analyses. Published methods of REGR profile determination from marking experiments fall in two classes: the profile is either described by a series of segmental growth rates, or calcul...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.113.4.1395
更新日期:1997-04-01 00:00:00
abstract::In C3 plants large amounts of photorespiratory glycine (Gly) are converted to serine by the tetrahydrofolate (THF)-dependent activities of the Gly decarboxylase complex (GDC) and serine hydroxymethyltransferase (SHMT). Using 13C nuclear magnetic resonance, we monitored the flux of carbon through the GDC/SHMT enzyme sy...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.116.1.137
更新日期:1998-01-01 00:00:00
abstract::The influence of ozone on Ca(2+) transport in plant membranes from pinto bean (Phaseolus vulgaris L. var Pinto) leaves was investigated in vitro by means of a filtration method using purified vesicles. Two transport mechanisms located at the plasma membrane are involved in a response to ozone: (a) passive Ca(2+) influ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.94.2.788
更新日期:1990-10-01 00:00:00
abstract::Peroxygenase-catalyzed epoxidation of oleic acid in preparations of cereal seeds was investigated. The 105,000g particle fraction of oat (Avena sativa) seed homogenate showed high peroxygenase activity, i.e. 3034 [plus or minus] 288 and 2441 [plus or minus] 168 nmol (10 min)-1 mg-1 protein in two cultivars, whereas th...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.110.3.807
更新日期:1996-03-01 00:00:00
abstract::Previous evidence has suggested that SPA1 is a signal transduction component that appears to require phytochrome A for function in seedling photomorphogenesis. Using digital image analysis, we examined the time course of growth inhibition induced by red light in spa1 mutants to test the interpretation that SPA1 functi...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.126.3.1291
更新日期:2001-07-01 00:00:00