Enhancement of plant-microbe interactions using a rhizosphere metabolomics-driven approach and its application in the removal of polychlorinated biphenyls.

Abstract:

:Persistent organic pollutants, such as polychlorinated biphenyls (PCBs), are a global problem. We demonstrate enhanced depletion of PCBs using root-associated microbes, which can use plant secondary metabolites, such as phenylpropanoids. Using a "rhizosphere metabolomics" approach, we show that phenylpropanoids constitute 84% of the secondary metabolites exuded from Arabidopsis roots. Phenylpropanoid-utilizing microbes are more competitive and are able to grow at least 100-fold better than their auxotrophic mutants on roots of plants that are able to synthesize or overproduce phenylpropanoids, such as flavonoids. Better colonization of the phenylpropanoid-utilizing strain in a gnotobiotic system on the roots of flavonoid-producing plants leads to almost 90% removal of PCBs in a 28-d period. Our work complements previous approaches to engineer soil microbial populations based on opines produced by transgenic plants and used by microbes carrying opine metabolism genes. The current approach based on plant natural products can be applied to contaminated soils with pre-existing vegetation. This strategy is also likely to be applicable to improving the competitive abilities of biocontrol and biofertilization strains.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Narasimhan K,Basheer C,Bajic VB,Swarup S

doi

10.1104/pp.102.016295

subject

Has Abstract

pub_date

2003-05-01 00:00:00

pages

146-53

issue

1

eissn

0032-0889

issn

1532-2548

journal_volume

132

pub_type

杂志文章
  • Peroxisomal degradation of branched-chain 2-oxo acids.

    abstract::Branched-chain 2-oxo acids which are formed by transamination of leucine, isoleucine, and valine are metabolized by the peroxisomes from mung bean (Vigna radiata L.) hypocotyls. Acylcoenzyme A (CoA) thio ester intermediates of the pathways were separated by reversed-phase high performance liquid chromatography. Retent...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.91.4.1387

    authors: Gerbling H,Gerhardt B

    更新日期:1989-12-01 00:00:00

  • Saponin biosynthesis in Saponaria vaccaria. cDNAs encoding beta-amyrin synthase and a triterpene carboxylic acid glucosyltransferase.

    abstract::Saponaria vaccaria (Caryophyllaceae), a soapwort, known in western Canada as cowcockle, contains bioactive oleanane-type saponins similar to those found in soapbark tree (Quillaja saponaria; Rosaceae). To improve our understanding of the biosynthesis of these saponins, a combined polymerase chain reaction and expresse...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.106.088484

    authors: Meesapyodsuk D,Balsevich J,Reed DW,Covello PS

    更新日期:2007-02-01 00:00:00

  • Carotenoid Biosynthesis during Tomato Fruit Development (Evidence for Tissue-Specific Gene Expression).

    abstract::Tomato (Lycopersicon esculentum Mill. cv Ailsa Craig) fruit, at five stages of development, have been analyzed for their carotenoid and chlorophyll (Chl) contents, in vitro activities of phytoene synthase, phytoene desaturase, and lycopene cyclase, as well as expression of the phytoene synthase (Psy) and phytoene desa...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.105.1.405

    authors: Fraser PD,Truesdale MR,Bird CR,Schuch W,Bramley PM

    更新日期:1994-05-01 00:00:00

  • Abscisic acid-induced resistance against the brown spot pathogen Cochliobolus miyabeanus in rice involves MAP kinase-mediated repression of ethylene signaling.

    abstract::The plant hormone abscisic acid (ABA) is involved in an array of plant processes, including the regulation of gene expression during adaptive responses to various environmental cues. Apart from its well-established role in abiotic stress adaptation, emerging evidence indicates that ABA is also prominently involved in ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.109.152702

    authors: De Vleesschauwer D,Yang Y,Cruz CV,Höfte M

    更新日期:2010-04-01 00:00:00

  • Rice cytokinin GATA transcription Factor1 regulates chloroplast development and plant architecture.

    abstract::Chloroplast biogenesis has been well documented in higher plants, yet the complex methods used to regulate chloroplast activity under fluctuating environmental conditions are not well understood. In rice (Oryza sativa), the CYTOKININ-RESPONSIVE GATA TRANSCRIPTION FACTOR1 (Cga1) shows increased expression following lig...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.113.217265

    authors: Hudson D,Guevara DR,Hand AJ,Xu Z,Hao L,Chen X,Zhu T,Bi YM,Rothstein SJ

    更新日期:2013-05-01 00:00:00

  • Regulation of Pyrimidine Biosynthesis in Intact Cells of Cucurbita pepo.

    abstract::The occurrence of the complete orotic acid pathway for the biosynthesis de novo of pyrimidine nucleotides was demonstrated in the intact cells of roots excised from summer squash (Cucurbita pepo L. cv. Early Prolific Straightneck). Evidence that the biosynthesis of pyrimidine nucleotides proceeds via the orotate pathw...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.64.4.562

    authors: Lovatt CJ,Albert LS

    更新日期:1979-10-01 00:00:00

  • Phloem loading in two Scrophulariaceae species. What can drive symplastic flow via plasmodesmata?

    abstract::To determine the driving forces for symplastic sugar flux between mesophyll and phloem, gradients of sugar concentrations and osmotic pressure were studied in leaf tissues of two Scrophulariaceae species, Alonsoa meridionalis and Asarina barclaiana. A. meridionalis has a typical symplastic configuration of minor-vein ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.105.068312

    authors: Voitsekhovskaja OV,Koroleva OA,Batashev DR,Knop C,Tomos AD,Gamalei YV,Heldt HW,Lohaus G

    更新日期:2006-01-01 00:00:00

  • Purification and partial characterization of a membrane-associated lipoxygenase in tomato fruit.

    abstract::Membrane-associated lipoxygenase from green tomato (Lycopersicon esculentum L. cv Caruso) fruit has been purified 49-fold to a specific activity of 8.3 mumol.min(-1).mg(-1) of protein by solubilization of microsomal membranes with Triton X-100, followed by anion- exchange and size-exclusion chromatography. The apparen...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.100.4.1802

    authors: Bowsher CG,Ferrie BJ,Ghosh S,Todd J,Thompson JE,Rothstein SJ

    更新日期:1992-12-01 00:00:00

  • Phosphatidylcholine Synthesis in Castor Bean Endosperm : I. Metabolism of l-Serine.

    abstract::Endosperm halves from 3-day-old castor bean (Ricinus communis var Hale) were incubated for 30 minutes with l-[(14)C]serine, after which label was observed in ethanolamine, choline, phosphatidylserine, phosphatidylethanolamine, phosphatidylcholine, ethanolaminephosphate, and CDPethanolamine, but not in cholinephosphate...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.84.1.78

    authors: Kinney AJ,Moore TS

    更新日期:1987-05-01 00:00:00

  • Phytoluminographic detection of dynamic variations in leaf gaseous conductivity.

    abstract::Gas exchange and plant luminescence (delayed light emission) of a single red kidney bean leaf undergoing synchronous oscillations in gas exchange were recorded and analyzed. Introduction of 1.1 microliter per liter SO(2) during these oscillations produced increases in plant luminescence that, when averaged over a port...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.78.4.904

    authors: Ellenson JL

    更新日期:1985-08-01 00:00:00

  • Identification of negative cis-acting elements in response to copper in the chloroplastic iron superoxide dismutase gene of the moss Barbula unguiculata.

    abstract::Superoxide dismutases (SODs) are ubiquitous metalloenzymes that catalyze the dismutation of superoxide radicals. Chloroplasts have two isozymes, copper/zinc SOD (Cu/ZnSOD) and iron SOD (FeSOD), encoded by nuclear genes. Because bryophytes are considered as the earliest land plants, they are one of the most interesting...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.107.114868

    authors: Nagae M,Nakata M,Takahashi Y

    更新日期:2008-04-01 00:00:00

  • Bacitracin Inhibits the Synthesis of Lipid-linked Saccharides and Glycoproteins in Plants.

    abstract::The particulate enzyme fraction from mung bean (Phaseolus aureus) seedlings catalyzes the incorporation of mannose from GDP-[(14)C]mannose into mannosyl-phosphoryl-dolichol and of N-acetylglucosamine from UDP-[(3)H]N-acetylglucosamine into N-acetylglucosamine-pyrophosphoryl-polyisoprenol. Bacitracin inhibits the trans...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.62.3.373

    authors: Ericson MC,Gafford J,Elbein AD

    更新日期:1978-09-01 00:00:00

  • A common fungal associate of the spruce bark beetle metabolizes the stilbene defenses of Norway spruce.

    abstract::Norway spruce (Picea abies) forests suffer periodic fatal attacks by the bark beetle Ips typographus and its fungal associate, Ceratocystis polonica. Norway spruce protects itself against fungal and bark beetle invasion by the production of terpenoid resins, but it is unclear whether resins or other defenses are effec...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.113.218610

    authors: Hammerbacher A,Schmidt A,Wadke N,Wright LP,Schneider B,Bohlmann J,Brand WA,Fenning TM,Gershenzon J,Paetz C

    更新日期:2013-07-01 00:00:00

  • Radioactive gibberellin a(5) and its metabolism in dwarf peas.

    abstract::Radioactive gibberellin A(5) ((3)H-GA(5)) was synthesized from gibberellic acid. When it was applied to dwarf peas grown in the dark, an average of 3% was converted to another acid gibberellin within 48 hours. The biological activity of the metabolite did not account for the response to applied GA(5). GA(5) is therefo...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.45.1.56

    authors: Musgrave A,Kende H

    更新日期:1970-01-01 00:00:00

  • New lv Mutants of Pea Are Deficient in Phytochrome B.

    abstract::The lv-1 mutant of pea (Pisum sativum L.) is deficient in responses regulated by phytochrome B (phyB) in other species but has normal levels of spectrally active phyB. We have characterized three further lv mutants (lv-2, lv-3, and lv-4), which are all elongated under red (R) and white light but are indistinguishable ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.108.2.525

    authors: Weller JL,Nagatani A,Kendrick RE,Murfet IC,Reid JB

    更新日期:1995-06-01 00:00:00

  • Environmental and genetic components of stomatal behavior in two genotypes of upland cotton.

    abstract::Two parental lines of Upland cotton (Gossypium hirsutum L.) plus their reciprocal F(1), F(2), and backcross populations were field-tested for environmental and genetic components of leaf diffusive resistance. Leaf resistance was measured with a diffusion porometer three times each day during 6 days in August. A large,...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.59.3.354

    authors: Roark B,Quisenberry JE

    更新日期:1977-03-01 00:00:00

  • Growth, Gravitropism, and Endogenous Ion Currents of Cress Roots (Lepidium sativum L.) : Measurements Using a Novel Three-Dimensional Recording Probe.

    abstract::A novel, three-dimensional recording, vibrating probe was used for measuring the density and direction of the endogenous ionic current of cress roots (Lepidium sativum L.) bathed in low salt media (artificial pond water, APW). Roots submerged in regular APW and growing vertically show the following current pattern. Cu...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.100.1.16

    authors: Weisenseel MH,Becker HF,Ehlgötz JG

    更新日期:1992-09-01 00:00:00

  • Isolation of a Putative Carboxysomal Carbonic Anhydrase Gene from the Cyanobacterium Synechococcus PCC7942.

    abstract::The Type II mutants of the cyanobacterium Synechococcus PCC7942 (G.D. Price, M.R. Badger [1989] Plant Physiol 91: 514-525) are able to accumulate a large pool of inorganic carbon inside the cell, but are unable to utilize it for CO(2) fixation, resulting in a high CO(2)-requiring phenotype. We have isolated a 3.5-kb B...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.100.2.794

    authors: Yu JW,Price GD,Song L,Badger MR

    更新日期:1992-10-01 00:00:00

  • Epigenetic transcriptional silencing and 5-azacytidine-mediated reactivation of a complex transgene in rice.

    abstract::Despite a growing number of reports indicating non-Mendelian inheritance of transgene expression in monocots, no detailed description of the structure and stability of the transgene exists for transformants generated by direct DNA-transfer techniques, making the cause for these observations difficult to determine. In ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.115.2.361

    authors: Kumpatla SP,Teng W,Buchholz WG,Hall TC

    更新日期:1997-10-01 00:00:00

  • Glutamine Synthetase/Glutamine: alpha-Ketoglutarate Aminotransferase in Chloroplasts from the Marine Alga Caulerpa simpliciuscula.

    abstract::The enzymic capacities for ammonia assimilation into amino acids have been investigated in chloroplasts from the siphonous green alga Caulerpa simpliciuscula (Turner) C. Ag. The results show that these chloroplasts differ from those of higher plants in having present simultaneously the enzymic capacities to permit ass...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.63.3.578

    authors: McKenzie GH,Ch'ng AL,Gayler KR

    更新日期:1979-03-01 00:00:00

  • Early effects of boron deficiency on indoleacetic Acid oxidase levels of squash root tips.

    abstract::The indoleacetic acid (IAA) oxidase activity of root tips of boron-sufficient, -deficient, recovering, and IAA-treated boron-sufficient squash plants (Cucurbita pepo L.) was determined. Apical and subapical root sections displayed an increase in IAA oxidase activity between 6 and 9 hours after boron was withheld, and ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.59.6.1047

    authors: Bohnsack CW,Albert LS

    更新日期:1977-06-01 00:00:00

  • Modification of Sulfhydryl Groups in the [gamma]-Subunit of Chloroplast-Coupling Factor 1 Affects the Proton Slip through the ATP Synthase.

    abstract::A large proton leak not coupled to ATP synthesis (slip) occurs at alkaline pH through the chloroplast ATP synthase (Y. Evron, M. Avron [1990] Biochim Biophys Acta 1019: 115-120). The involvement of the ATP synthase [gamma]-subunit in the regulation of proton conductance was analyzed by measuring the effect of thiolalk...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.115.4.1549

    authors: Evron Y,Pick U

    更新日期:1997-12-01 00:00:00

  • SHORT-ROOT and SCARECROW regulate leaf growth in Arabidopsis by stimulating S-phase progression of the cell cycle.

    abstract::SHORT-ROOT (SHR) and SCARECROW (SCR) are required for stem cell maintenance in the Arabidopsis (Arabidopsis thaliana) root meristem, ensuring its indeterminate growth. Mutation of SHR and SCR genes results in disorganization of the quiescent center and loss of stem cell activity, resulting in the cessation of root gro...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.110.158857

    authors: Dhondt S,Coppens F,De Winter F,Swarup K,Merks RM,Inzé D,Bennett MJ,Beemster GT

    更新日期:2010-11-01 00:00:00

  • Properties of a Chloroplast Enzyme that Cleaves the Chlorophyll a/b Binding Protein Precursor : Optimization of an Organelle-Free Reaction.

    abstract::The major light-harvesting chlorophyll a/b binding protein (LHCP) of higher plant chloroplasts is nuclear-encoded, synthesized as a precursor, and processed upon import. We have previously (GK Lamppa, M Abad [1987] J Cell Biol 105: 2641-2648) identified a soluble enzyme that cleaves the LHCP precursor (pLHCP). In this...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.90.1.117

    authors: Abad MS,Clark SE,Lamppa GK

    更新日期:1989-05-01 00:00:00

  • An Arabidopsis prenylated Rab acceptor 1 isoform, AtPRA1.B6, displays differential inhibitory effects on anterograde trafficking of proteins at the endoplasmic reticulum.

    abstract::Prenylated Rab acceptors (PRAs), members of the Ypt-interacting protein family of small membrane proteins, are thought to aid the targeting of prenylated Rabs to their respective endomembrane compartments. In plants, the Arabidopsis (Arabidopsis thaliana) PRA1 family contains 19 members that display varying degrees of...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.111.180810

    authors: Lee MH,Jung C,Lee J,Kim SY,Lee Y,Hwang I

    更新日期:2011-10-01 00:00:00

  • mEosFP-based green-to-red photoconvertible subcellular probes for plants.

    abstract::Photoconvertible fluorescent proteins (FPs) are recent additions to the biologists' toolbox for understanding the living cell. Like green fluorescent protein (GFP), monomeric EosFP is bright green in color but is efficiently photoconverted into a red fluorescent form using a mild violet-blue excitation. Here, we repor...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.110.165431

    authors: Mathur J,Radhamony R,Sinclair AM,Donoso A,Dunn N,Roach E,Radford D,Mohaghegh PS,Logan DC,Kokolic K,Mathur N

    更新日期:2010-12-01 00:00:00

  • Influence of Iron Chlorosis on Pigment and Protein Metabolism in Leaves of Nicotiana tabacum L.

    abstract::Experiments were conducted on Nicotiana tabacum, L. to study the relation in the grana among chlorophylls, carotenoids, and proteins. The effect of iron chlorosis on protein and pigment synthesis was studied at different stages of chlorosis using glycine-U-C(14). Pigments were separated by thin layer chromatography.Ch...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.41.3.415

    authors: Shetty AS,Miller GW

    更新日期:1966-03-01 00:00:00

  • Localization, solubilization and characterization of plant membrane-associated calcium-dependent protein kinases.

    abstract::Membrane fractions from mature silver beet (Beta vulgaris) deveined leaf and leaf stem homogenates have associated Ca(2+) -dependent protein kinase. The Ca(2+) -dependent protein kinase activity is associated with plasma membranes (density 1.14-1.18 grams per cubic centimeter) as determined from copurification on isop...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.88.1.164

    authors: Klucis E,Polya GM

    更新日期:1988-09-01 00:00:00

  • The decisive step in betaxanthin biosynthesis is a spontaneous reaction1

    abstract::Experiments were performed to confirm that the aldimine bond formation is a spontaneous reaction, because attempts to find an enzyme catalyzing the last decisive step in betaxanthin biosynthesis, the aldimine formation, failed. Feeding different amino acids to betalain-forming hairy root cultures of yellow beet (Beta ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.119.4.1217

    authors: Schliemann W,Kobayashi N,Strack D

    更新日期:1999-04-01 00:00:00

  • ADP/ATP Translocator from Pea Root Plastids (Comparison with Translocators from Spinach Chloroplasts and Pea Leaf Mitochondria).

    abstract::The kinetic properties of the adenosine 5[prime]-diphosphate/adenosine 5[prime]-triphosphate (ADP/ATP) translocator from pea (Pisum sativum L.) root plastids were determined by silicone oil filtering centrifugation and compared with those of spinach (Spinacia oleracea L.) chloroplasts and pea leaf mitochondria. In add...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.103.1.131

    authors: Schunemann D,Borchert S,Flugge UI,Heldt HW

    更新日期:1993-09-01 00:00:00