Abstract:
:Branched-chain 2-oxo acids which are formed by transamination of leucine, isoleucine, and valine are metabolized by the peroxisomes from mung bean (Vigna radiata L.) hypocotyls. Acylcoenzyme A (CoA) thio ester intermediates of the pathways were separated by reversed-phase high performance liquid chromatography. Retention time and cochromatography of individual acyl-CoA reference standards were used for identification of the acyl-CoA esters separated from the assay mixtures. Based on the results of identification and those of kinetic experiments, pathways of the peroxisomal degradation of 2-oxoisocaproate, 2-oxoisovalerate, and 2-oxo-3-methylvalerate are suggested.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Gerbling H,Gerhardt Bdoi
10.1104/pp.91.4.1387subject
Has Abstractpub_date
1989-12-01 00:00:00pages
1387-92issue
4eissn
0032-0889issn
1532-2548journal_volume
91pub_type
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