Peroxisomal degradation of branched-chain 2-oxo acids.

Abstract:

:Branched-chain 2-oxo acids which are formed by transamination of leucine, isoleucine, and valine are metabolized by the peroxisomes from mung bean (Vigna radiata L.) hypocotyls. Acylcoenzyme A (CoA) thio ester intermediates of the pathways were separated by reversed-phase high performance liquid chromatography. Retention time and cochromatography of individual acyl-CoA reference standards were used for identification of the acyl-CoA esters separated from the assay mixtures. Based on the results of identification and those of kinetic experiments, pathways of the peroxisomal degradation of 2-oxoisocaproate, 2-oxoisovalerate, and 2-oxo-3-methylvalerate are suggested.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Gerbling H,Gerhardt B

doi

10.1104/pp.91.4.1387

subject

Has Abstract

pub_date

1989-12-01 00:00:00

pages

1387-92

issue

4

eissn

0032-0889

issn

1532-2548

journal_volume

91

pub_type

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