Abstract:
:Rootless soybean (Glycine max) seedlings were used as a test system to examine the action of auxin on chromatin-directed RNA synthesis. Chromatin from the basal tissue of rootless seedlings (both control and auxin-treated) had RNA synthetic capacity similar to that of chromatin from comparably treated intact seedlings. When DNA synthesis normally induced in the basal tissue by auxin was blocked in the rootless seedlings by 5-fluorodeoxyuridine, the auxin enhancement of chromatin activity was inhibited 70%. This level was still three times the control level, indicating that auxin influenced the synthetic activity of existing DNA template. Experiments with Escherichia coli RNA polymerase revealed that chromatin from both auxin- and auxin plus 5-fluorodeoxyuridine-treated tissue saturated at higher levels than chromatin from control tissue.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Holm RE,Key JLdoi
10.1104/pp.47.5.606subject
Has Abstractpub_date
1971-05-01 00:00:00pages
606-8issue
5eissn
0032-0889issn
1532-2548journal_volume
47pub_type
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