Osmotic effects on the electrical properties of Arabidopsis root hair vacuoles in situ.

Abstract:

:To assess the role of the vacuole in responses to hyperosmotic and hypo-osmotic stress, the electrical properties of the vacuole were measured in situ. A double-barrel micropipette was inserted into the vacuole for voltage clamping. A second double-barrel micropipette was inserted into the cytoplasm to provide a virtual ground that separated the electrical properties of the vacuole from those of the plasma membrane. Osmotic stress causes immediate electrical responses at the plasma membrane (Lew RR [1996] Plant Physiol 97: 2002-2005) and ion flux changes and turgor recovery (Shabala SN, Lew RR [2002] 129: 290-299) in Arabidopsis root cells. In situ, the vacuole also responds rapidly to changes in extracellular osmotic potential. Hyperosmotic treatment caused a very large increase in the ionic conductance of the vacuole. Hypo-osmotic treatment did not affect the vacuolar conductance. In either case, the vacuolar electrical potential was unchanged. Taken in concert with previous studies of changes at the plasma membrane, these results demonstrate a highly coordinated system in which the vacuole and plasma membrane are primed to respond immediately to hyperosmotic stress before changes in gene expression.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Lew RR

doi

10.1104/pp.103.031427

subject

Has Abstract

pub_date

2004-01-01 00:00:00

pages

352-60

issue

1

eissn

0032-0889

issn

1532-2548

pii

134/1/352

journal_volume

134

pub_type

杂志文章
  • Genetic and genomic evidence that sucrose is a global regulator of plant responses to phosphate starvation in Arabidopsis.

    abstract::Plants respond to phosphate (Pi) starvation by exhibiting a suite of developmental, biochemical, and physiological changes to cope with this nutritional stress. To understand the molecular mechanism underlying these responses, we isolated an Arabidopsis (Arabidopsis thaliana) mutant, hypersensitive to phosphate starva...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.110.171736

    authors: Lei M,Liu Y,Zhang B,Zhao Y,Wang X,Zhou Y,Raghothama KG,Liu D

    更新日期:2011-07-01 00:00:00

  • C-Photosynthate Partitioning and Translocation in Soybeans during Reproductive Development.

    abstract::Partitioning and translocation of (14)C-photosynthates were examined during flowering and seed maturation in soybean (Glycine max [L.]Merr.) plants to quantify allocation to sugars, amino acids, organic acids, and starch and to study transport of C and N from leaves to reproductive sinks. The trifoliolate leaf at the ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.75.4.1040

    authors: Koch KE,Schrader LE

    更新日期:1984-08-01 00:00:00

  • AtATM3 is involved in heavy metal resistance in Arabidopsis.

    abstract::AtATM3, an ATP-binding cassette transporter of Arabidopsis (Arabidopsis thaliana), is a mitochondrial protein involved in the biogenesis of iron-sulfur clusters and iron homeostasis in plants. Our gene expression analysis showed that AtATM3 is up-regulated in roots of plants treated with cadmium [Cd(II)] or lead (II);...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.105.074146

    authors: Kim DY,Bovet L,Kushnir S,Noh EW,Martinoia E,Lee Y

    更新日期:2006-03-01 00:00:00

  • Tonoplast stability and survival of isolated vacuoles in different buffers.

    abstract::The mechanism of sucrose transport into vacuoles isolated from leaf tissue has been studied only in barley (Hordeum vulgare) mesophyll cells. In this tissue, sucrose transport was reported to be a facilitated diffusion. We have observed a facilitated diffusion of sucrose into vacuoles isolated from this tissue. Howeve...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.88.2.251

    authors: De Leon JL,Daie J,Wyse R

    更新日期:1988-10-01 00:00:00

  • Looking deep inside: detection of low-abundance proteins in leaf extracts of Arabidopsis and phloem exudates of pumpkin.

    abstract::The field of proteomics suffers from the immense complexity of even small proteomes and the enormous dynamic range of protein concentrations within a given sample. Most protein samples contain a few major proteins, which hamper in-depth proteomic analysis. In the human field, combinatorial hexapeptide ligand libraries...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.112.198077

    authors: Fröhlich A,Gaupels F,Sarioglu H,Holzmeister C,Spannagl M,Durner J,Lindermayr C

    更新日期:2012-07-01 00:00:00

  • The hpa1 mutant of Arabidopsis reveals a crucial role of histidine homeostasis in root meristem maintenance.

    abstract::Histidine (His) is an essential ingredient for protein synthesis and is required by all living organisms. In higher plants, although there is considerable evidence that His is essential for plant growth and survival, there is very little information as to whether it plays any specific role in plant development. Here, ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.106.084178

    authors: Mo X,Zhu Q,Li X,Li J,Zeng Q,Rong H,Zhang H,Wu P

    更新日期:2006-08-01 00:00:00

  • Low Temperature Enhances Plant Immunity via Salicylic Acid Pathway Genes That Are Repressed by Ethylene.

    abstract::Temperature has a large impact on plant immune responses. Earlier studies identified intracellular immune receptor nucleotide-binding leucine-rich repeat (NLR) genes and salicylic acid (SA) as targets of high-temperature inhibition of plant immunity. Here, we report that moderately low temperature enhances immunity to...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.19.01130

    authors: Li Z,Liu H,Ding Z,Yan J,Yu H,Pan R,Hu J,Guan Y,Hua J

    更新日期:2020-01-01 00:00:00

  • Isolation of a Putative Carboxysomal Carbonic Anhydrase Gene from the Cyanobacterium Synechococcus PCC7942.

    abstract::The Type II mutants of the cyanobacterium Synechococcus PCC7942 (G.D. Price, M.R. Badger [1989] Plant Physiol 91: 514-525) are able to accumulate a large pool of inorganic carbon inside the cell, but are unable to utilize it for CO(2) fixation, resulting in a high CO(2)-requiring phenotype. We have isolated a 3.5-kb B...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.100.2.794

    authors: Yu JW,Price GD,Song L,Badger MR

    更新日期:1992-10-01 00:00:00

  • A Noninvasive Technique for Monitoring Peroxidative and H2O2-Scavenging Activities during Interactions between Bacterial Plant Pathogens and Suspension Cells.

    abstract::Stimulation of active oxygen metabolism occurs during the early stages of interactions involving bacteria and plant cell suspensions. Although many cellular processes are known to affect active oxygen metabolism in plants, it is not known which of these factors affect active oxygen levels during plant-bacteria interac...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.108.1.353

    authors: Baker CJ,Harmon GL,Glazener JA,Orlandi EW

    更新日期:1995-05-01 00:00:00

  • Plastidic Δ6 Fatty-Acid Desaturases with Distinctive Substrate Specificity Regulate the Pool of C18-PUFAs in the Ancestral Picoalga Ostreococcus tauri.

    abstract::Eukaryotic Δ6-desaturases are microsomal enzymes that balance the synthesis of ω-3 and ω-6 C18-polyunsaturated fatty acids (C18-PUFAs) according to their specificity. In several microalgae, including Ostreococcus tauri, plastidic C18-PUFAs are strictly regulated by environmental cues suggesting an autonomous control o...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.20.00281

    authors: Degraeve-Guilbault C,Gomez RE,Lemoigne C,Pankansem N,Morin S,Tuphile K,Joubès J,Jouhet J,Gronnier J,Suzuki I,Coulon D,Domergue F,Corellou F

    更新日期:2020-09-01 00:00:00

  • Preparation of Membrane Vesicles Enriched in ATP-Dependent Proton Transport from Suspension Cultures of Tomato Cells.

    abstract::Membranes enriched in ATP-dependent proton transport were prepared from suspension cultures of tomato cells (Lycopersicon esculentum Mill cv VF36). Suspension cultures were a source of large quantities of membranes from rapidly growing, undifferentiated cells. Proton transport activity was assayed as quench of acridin...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.77.1.69

    authors: Dupont FM,Zabala Mde G

    更新日期:1985-01-01 00:00:00

  • In vitro biosynthesis of phosphorylated starch in intact potato amyloplasts

    abstract::Intact amyloplasts from potato (Solanum tuberosum L.) were used to study starch biosynthesis and phosphorylation. Assessed by the degree of intactness and by the level of cytosolic and vacuolar contamination, the best preparations were selected by searching for amyloplasts containing small starch grains. The isolated,...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.119.2.455

    authors: Wischmann B,Hamborg Nielsen T,Lindberg Moller B

    更新日期:1999-02-01 00:00:00

  • An arginyl-transfer ribonucleic Acid protein transferase from cereal embryos.

    abstract::Embryos from rice (Oryza sativa L. var. Bluebonnet) and wheat (Triticum aestivum L.) contain an aminoacyl-tRNA protein transferase which transfers arginine from arginyl-tRNA to the N terminus of a protein acceptor. The activity was measured in vitro in a reaction mixture containing embryo supernatant fraction, buffer,...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.52.1.13

    authors: Manahan CO,App AA

    更新日期:1973-07-01 00:00:00

  • AtNUDX6, an ADP-ribose/NADH pyrophosphohydrolase in Arabidopsis, positively regulates NPR1-dependent salicylic acid signaling.

    abstract::Here, we investigated the physiological role of Arabidopsis (Arabidopsis thaliana) AtNUDX6, the gene encoding ADP-ribose (Rib)/NADH pyrophosphohydrolase, using its overexpressor (Pro35S:AtNUDX6) or disruptant (KO-nudx6). The level of NADH in Pro35S:AtNUDX6 and KO-nudx6 plants was decreased and increased, respectively,...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.110.153569

    authors: Ishikawa K,Yoshimura K,Harada K,Fukusaki E,Ogawa T,Tamoi M,Shigeoka S

    更新日期:2010-04-01 00:00:00

  • Specificity of RCN1-mediated protein phosphatase 2A regulation in meristem organization and stress response in roots.

    abstract::Protein dephosphorylation by the serine/threonine protein phosphatase 2A (PP2A) modulates a broad array of cellular functions. PP2A normally acts as a heterotrimeric holoenzyme complex comprising a catalytic subunit bound by regulatory A and B subunits. Characterization of the regulatory A subunit isoforms (ROOTS CURL...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.107.112995

    authors: Blakeslee JJ,Zhou HW,Heath JT,Skottke KR,Barrios JA,Liu SY,DeLong A

    更新日期:2008-02-01 00:00:00

  • BIN2, a new brassinosteroid-insensitive locus in Arabidopsis.

    abstract::Brassinosteroids (BRs) play important roles throughout plant development. Although many genes have been identified that are involved in BR biosynthesis, genetic approaches in Arabidopsis have led to the identification of only one gene, BRI1, that encodes a membrane receptor for BRs. To expand our knowledge of the mole...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.127.1.14

    authors: Li J,Nam KH,Vafeados D,Chory J

    更新日期:2001-09-01 00:00:00

  • Changes in biochemical composition of the cell wall of the cotton fiber during development.

    abstract::The composition of the cell wall of the cotton fiber (Gossypium hirsutum L. Acala SJ-1) has been studied from the early stages of elongation (5 days postanthesis) through the period of secondary wall formation, using cell walls derived both from fibers developing on the plant and from fibers obtained from excised, cul...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.59.6.1088

    authors: Meinert MC,Delmer DP

    更新日期:1977-06-01 00:00:00

  • Stable transformation of ferns using spores as targets: Pteris vittata and Ceratopteris thalictroides.

    abstract::Ferns (Pteridophyta) are very important members of the plant kingdom that lag behind other taxa with regards to our understanding of their genetics, genomics, and molecular biology. We report here, to our knowledge, the first instance of stable transformation of fern with recovery of transgenic sporophytes. Spores of ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.113.224675

    authors: Muthukumar B,Joyce BL,Elless MP,Stewart CN Jr

    更新日期:2013-10-01 00:00:00

  • Ionic Current Changes Associated with the Gravity-Induced Bending Response in Roots of Zea mays L.

    abstract::A vibrating probe was used to measure the changes in ionic currents around gravistimulated roots of Zea mays L. in an effort to determine whether these currents are involved in stimulus transduction from the root cap to the elongation zone. We did not observe a migration of the previously reported auxin-insensitive cu...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.100.3.1417

    authors: Collings DA,White RG,Overall RL

    更新日期:1992-11-01 00:00:00

  • Phytoluminographic detection of dynamic variations in leaf gaseous conductivity.

    abstract::Gas exchange and plant luminescence (delayed light emission) of a single red kidney bean leaf undergoing synchronous oscillations in gas exchange were recorded and analyzed. Introduction of 1.1 microliter per liter SO(2) during these oscillations produced increases in plant luminescence that, when averaged over a port...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.78.4.904

    authors: Ellenson JL

    更新日期:1985-08-01 00:00:00

  • The Unfolded Protein Response Modulates a Phosphoinositide-Binding Protein through the IRE1-bZIP60 Pathway.

    abstract::Phosphoinositides function as lipid signals in plant development and stress tolerance by binding with partner proteins. We previously reported that Arabidopsis (Arabidopsis thaliana) phosphoinositide-specific phospholipase C2 functions in the endoplasmic reticulum (ER) stress response. However, the underlying molecula...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.19.01488

    authors: Yu CY,Kanehara K

    更新日期:2020-05-01 00:00:00

  • The protein kinase SnRK2.6 mediates the regulation of sucrose metabolism and plant growth in Arabidopsis.

    abstract::In higher plants, three subfamilies of sucrose nonfermenting-1 (Snf1)-related protein kinases have evolved. While the Snf1-related protein kinase 1 (SnRK1) subfamily has been shown to share pivotal roles with the orthologous yeast Snf1 and mammalian AMP-activated protein kinase in modulating energy and metabolic homeo...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.109.150789

    authors: Zheng Z,Xu X,Crosley RA,Greenwalt SA,Sun Y,Blakeslee B,Wang L,Ni W,Sopko MS,Yao C,Yau K,Burton S,Zhuang M,McCaskill DG,Gachotte D,Thompson M,Greene TW

    更新日期:2010-05-01 00:00:00

  • Identification and characterization of nucleotide-binding site-leucine-rich repeat genes in the model plant Medicago truncatula.

    abstract::The nucleotide-binding site (NBS)-Leucine-rich repeat (LRR) gene family accounts for the largest number of known disease resistance genes, and is one of the largest gene families in plant genomes. We have identified 333 nonredundant NBS-LRRs in the current Medicago truncatula draft genome (Mt1.0), likely representing ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.107.104588

    authors: Ameline-Torregrosa C,Wang BB,O'Bleness MS,Deshpande S,Zhu H,Roe B,Young ND,Cannon SB

    更新日期:2008-01-01 00:00:00

  • Partitioning of Intermediary Carbon Metabolism in Vesicular-Arbuscular Mycorrhizal Leek.

    abstract::Vesicular-arbuscular mycorrhizal fungi are symbionts for a large variety of crop plants; however, the form in which they take up carbon from the host is not established. To trace the course of carbon metabolism, we have used nuclear magnetic resonance spectroscopy with [13C]glucose labeling in vivo and in extracts to ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.108.1.7

    authors: Shachar-Hill Y,Pfeffer PE,Douds D,Osman SF,Doner LW,Ratcliffe RG

    更新日期:1995-05-01 00:00:00

  • Respiration and mitochondrial biogenesis in germinating embryos of maize.

    abstract::Function of the cyanide-sensitive mitochondrial electron transport system was required for germination of the Zea mays embryo. Respiration of the standard electron transport system (rather than the alternate oxidase) began immediately upon initiation of imbibition. This respiration depended upon cytochrome c oxidase a...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.93.1.295

    authors: Ehrenshaft M,Brambl R

    更新日期:1990-05-01 00:00:00

  • Differential induction of plant volatile biosynthesis in the lima bean by early and late intermediates of the octadecanoid-signaling pathway.

    abstract::Plants are able to respond to herbivore damage with de novo biosynthesis of an herbivore-characteristic blend of volatiles. The signal transduction initiating volatile biosynthesis may involve the activation of the octadecanoid pathway, as exemplified by the transient increase of endogenous jasmonic acid (JA) in leave...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.121.1.153

    authors: Koch T,Krumm T,Jung V,Engelberth J,Boland W

    更新日期:1999-09-01 00:00:00

  • The low-oxygen-induced NAC domain transcription factor ANAC102 affects viability of Arabidopsis seeds following low-oxygen treatment.

    abstract::Low-oxygen stress imposed by field waterlogging is a serious impediment to plant germination and growth. Plants respond to waterlogging with a complex set of physiological responses regulated at the transcriptional, cellular, and tissue levels. The Arabidopsis (Arabidopsis thaliana) NAC domain-containing gene ANAC102 ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.108.131912

    authors: Christianson JA,Wilson IW,Llewellyn DJ,Dennis ES

    更新日期:2009-04-01 00:00:00

  • Evaluation of h secretion relative to zeatin-induced growth of detached cucumber cotyledons.

    abstract::Cytokinins promote expansion of cotyledons detached from seedlings of more than a dozen species. The zeatin-enhanced expansion of cucumber (Cucumis sativus L. cv Marketer) cotyledons was investigated. In addition, whether acid secretion is involved in wall loosening accompanying such accelerated growth was evaluated. ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.70.5.1470

    authors: Ross CW,Rayle DL

    更新日期:1982-11-01 00:00:00

  • Reduction of light-induced anthocyanin accumulation in inoculated sorghum mesocotyls. Implications for a compensatory role in the defense response.

    abstract::Sorghum (Sorghum bicolor L. Moench) accumulates the anthocyanin cyanidin 3-dimalonyl glucoside in etiolated mesocotyls in response to light. Inoculation with the nonpathogenic fungus Cochliobolus heterostrophus drastically reduced the light-induced accumulation of anthocyanin by repressing the transcription of the ant...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.116.3.979

    authors: Lo SC,Nicholson RL

    更新日期:1998-03-01 00:00:00

  • NADP-Utilizing Enzymes in the Matrix of Plant Mitochondria.

    abstract::Purified potato tuber (Solanum tuberosum L. cv Bintie) mitochondria contain soluble, highly latent NAD(+)- and NADP(+)-isocitrate dehydrogenases, NAD(+)- and NADP(+)-malate dehydrogenases, as well as an NADPH-specific glutathione reductase (160, 25, 7200, 160, and 16 nanomoles NAD(P)H per minute and milligram protein,...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.94.3.1012

    authors: Rasmusson AG,Møller IM

    更新日期:1990-11-01 00:00:00