Abstract:
:To assess the role of the vacuole in responses to hyperosmotic and hypo-osmotic stress, the electrical properties of the vacuole were measured in situ. A double-barrel micropipette was inserted into the vacuole for voltage clamping. A second double-barrel micropipette was inserted into the cytoplasm to provide a virtual ground that separated the electrical properties of the vacuole from those of the plasma membrane. Osmotic stress causes immediate electrical responses at the plasma membrane (Lew RR [1996] Plant Physiol 97: 2002-2005) and ion flux changes and turgor recovery (Shabala SN, Lew RR [2002] 129: 290-299) in Arabidopsis root cells. In situ, the vacuole also responds rapidly to changes in extracellular osmotic potential. Hyperosmotic treatment caused a very large increase in the ionic conductance of the vacuole. Hypo-osmotic treatment did not affect the vacuolar conductance. In either case, the vacuolar electrical potential was unchanged. Taken in concert with previous studies of changes at the plasma membrane, these results demonstrate a highly coordinated system in which the vacuole and plasma membrane are primed to respond immediately to hyperosmotic stress before changes in gene expression.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Lew RRdoi
10.1104/pp.103.031427subject
Has Abstractpub_date
2004-01-01 00:00:00pages
352-60issue
1eissn
0032-0889issn
1532-2548pii
134/1/352journal_volume
134pub_type
杂志文章相关文献
PLANT PHYSIOLOGY文献大全abstract::Plants respond to phosphate (Pi) starvation by exhibiting a suite of developmental, biochemical, and physiological changes to cope with this nutritional stress. To understand the molecular mechanism underlying these responses, we isolated an Arabidopsis (Arabidopsis thaliana) mutant, hypersensitive to phosphate starva...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.110.171736
更新日期:2011-07-01 00:00:00
abstract::Partitioning and translocation of (14)C-photosynthates were examined during flowering and seed maturation in soybean (Glycine max [L.]Merr.) plants to quantify allocation to sugars, amino acids, organic acids, and starch and to study transport of C and N from leaves to reproductive sinks. The trifoliolate leaf at the ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.75.4.1040
更新日期:1984-08-01 00:00:00
abstract::AtATM3, an ATP-binding cassette transporter of Arabidopsis (Arabidopsis thaliana), is a mitochondrial protein involved in the biogenesis of iron-sulfur clusters and iron homeostasis in plants. Our gene expression analysis showed that AtATM3 is up-regulated in roots of plants treated with cadmium [Cd(II)] or lead (II);...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.105.074146
更新日期:2006-03-01 00:00:00
abstract::The mechanism of sucrose transport into vacuoles isolated from leaf tissue has been studied only in barley (Hordeum vulgare) mesophyll cells. In this tissue, sucrose transport was reported to be a facilitated diffusion. We have observed a facilitated diffusion of sucrose into vacuoles isolated from this tissue. Howeve...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.88.2.251
更新日期:1988-10-01 00:00:00
abstract::The field of proteomics suffers from the immense complexity of even small proteomes and the enormous dynamic range of protein concentrations within a given sample. Most protein samples contain a few major proteins, which hamper in-depth proteomic analysis. In the human field, combinatorial hexapeptide ligand libraries...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.112.198077
更新日期:2012-07-01 00:00:00
abstract::Histidine (His) is an essential ingredient for protein synthesis and is required by all living organisms. In higher plants, although there is considerable evidence that His is essential for plant growth and survival, there is very little information as to whether it plays any specific role in plant development. Here, ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.106.084178
更新日期:2006-08-01 00:00:00
abstract::Temperature has a large impact on plant immune responses. Earlier studies identified intracellular immune receptor nucleotide-binding leucine-rich repeat (NLR) genes and salicylic acid (SA) as targets of high-temperature inhibition of plant immunity. Here, we report that moderately low temperature enhances immunity to...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.19.01130
更新日期:2020-01-01 00:00:00
abstract::The Type II mutants of the cyanobacterium Synechococcus PCC7942 (G.D. Price, M.R. Badger [1989] Plant Physiol 91: 514-525) are able to accumulate a large pool of inorganic carbon inside the cell, but are unable to utilize it for CO(2) fixation, resulting in a high CO(2)-requiring phenotype. We have isolated a 3.5-kb B...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.100.2.794
更新日期:1992-10-01 00:00:00
abstract::Stimulation of active oxygen metabolism occurs during the early stages of interactions involving bacteria and plant cell suspensions. Although many cellular processes are known to affect active oxygen metabolism in plants, it is not known which of these factors affect active oxygen levels during plant-bacteria interac...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.108.1.353
更新日期:1995-05-01 00:00:00
abstract::Eukaryotic Δ6-desaturases are microsomal enzymes that balance the synthesis of ω-3 and ω-6 C18-polyunsaturated fatty acids (C18-PUFAs) according to their specificity. In several microalgae, including Ostreococcus tauri, plastidic C18-PUFAs are strictly regulated by environmental cues suggesting an autonomous control o...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.20.00281
更新日期:2020-09-01 00:00:00
abstract::Membranes enriched in ATP-dependent proton transport were prepared from suspension cultures of tomato cells (Lycopersicon esculentum Mill cv VF36). Suspension cultures were a source of large quantities of membranes from rapidly growing, undifferentiated cells. Proton transport activity was assayed as quench of acridin...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.77.1.69
更新日期:1985-01-01 00:00:00
abstract::Intact amyloplasts from potato (Solanum tuberosum L.) were used to study starch biosynthesis and phosphorylation. Assessed by the degree of intactness and by the level of cytosolic and vacuolar contamination, the best preparations were selected by searching for amyloplasts containing small starch grains. The isolated,...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.119.2.455
更新日期:1999-02-01 00:00:00
abstract::Embryos from rice (Oryza sativa L. var. Bluebonnet) and wheat (Triticum aestivum L.) contain an aminoacyl-tRNA protein transferase which transfers arginine from arginyl-tRNA to the N terminus of a protein acceptor. The activity was measured in vitro in a reaction mixture containing embryo supernatant fraction, buffer,...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.52.1.13
更新日期:1973-07-01 00:00:00
abstract::Here, we investigated the physiological role of Arabidopsis (Arabidopsis thaliana) AtNUDX6, the gene encoding ADP-ribose (Rib)/NADH pyrophosphohydrolase, using its overexpressor (Pro35S:AtNUDX6) or disruptant (KO-nudx6). The level of NADH in Pro35S:AtNUDX6 and KO-nudx6 plants was decreased and increased, respectively,...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.110.153569
更新日期:2010-04-01 00:00:00
abstract::Protein dephosphorylation by the serine/threonine protein phosphatase 2A (PP2A) modulates a broad array of cellular functions. PP2A normally acts as a heterotrimeric holoenzyme complex comprising a catalytic subunit bound by regulatory A and B subunits. Characterization of the regulatory A subunit isoforms (ROOTS CURL...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.107.112995
更新日期:2008-02-01 00:00:00
abstract::Brassinosteroids (BRs) play important roles throughout plant development. Although many genes have been identified that are involved in BR biosynthesis, genetic approaches in Arabidopsis have led to the identification of only one gene, BRI1, that encodes a membrane receptor for BRs. To expand our knowledge of the mole...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.127.1.14
更新日期:2001-09-01 00:00:00
abstract::The composition of the cell wall of the cotton fiber (Gossypium hirsutum L. Acala SJ-1) has been studied from the early stages of elongation (5 days postanthesis) through the period of secondary wall formation, using cell walls derived both from fibers developing on the plant and from fibers obtained from excised, cul...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.59.6.1088
更新日期:1977-06-01 00:00:00
abstract::Ferns (Pteridophyta) are very important members of the plant kingdom that lag behind other taxa with regards to our understanding of their genetics, genomics, and molecular biology. We report here, to our knowledge, the first instance of stable transformation of fern with recovery of transgenic sporophytes. Spores of ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.113.224675
更新日期:2013-10-01 00:00:00
abstract::A vibrating probe was used to measure the changes in ionic currents around gravistimulated roots of Zea mays L. in an effort to determine whether these currents are involved in stimulus transduction from the root cap to the elongation zone. We did not observe a migration of the previously reported auxin-insensitive cu...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.100.3.1417
更新日期:1992-11-01 00:00:00
abstract::Gas exchange and plant luminescence (delayed light emission) of a single red kidney bean leaf undergoing synchronous oscillations in gas exchange were recorded and analyzed. Introduction of 1.1 microliter per liter SO(2) during these oscillations produced increases in plant luminescence that, when averaged over a port...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.78.4.904
更新日期:1985-08-01 00:00:00
abstract::Phosphoinositides function as lipid signals in plant development and stress tolerance by binding with partner proteins. We previously reported that Arabidopsis (Arabidopsis thaliana) phosphoinositide-specific phospholipase C2 functions in the endoplasmic reticulum (ER) stress response. However, the underlying molecula...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.19.01488
更新日期:2020-05-01 00:00:00
abstract::In higher plants, three subfamilies of sucrose nonfermenting-1 (Snf1)-related protein kinases have evolved. While the Snf1-related protein kinase 1 (SnRK1) subfamily has been shown to share pivotal roles with the orthologous yeast Snf1 and mammalian AMP-activated protein kinase in modulating energy and metabolic homeo...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.109.150789
更新日期:2010-05-01 00:00:00
abstract::The nucleotide-binding site (NBS)-Leucine-rich repeat (LRR) gene family accounts for the largest number of known disease resistance genes, and is one of the largest gene families in plant genomes. We have identified 333 nonredundant NBS-LRRs in the current Medicago truncatula draft genome (Mt1.0), likely representing ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.107.104588
更新日期:2008-01-01 00:00:00
abstract::Vesicular-arbuscular mycorrhizal fungi are symbionts for a large variety of crop plants; however, the form in which they take up carbon from the host is not established. To trace the course of carbon metabolism, we have used nuclear magnetic resonance spectroscopy with [13C]glucose labeling in vivo and in extracts to ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.108.1.7
更新日期:1995-05-01 00:00:00
abstract::Function of the cyanide-sensitive mitochondrial electron transport system was required for germination of the Zea mays embryo. Respiration of the standard electron transport system (rather than the alternate oxidase) began immediately upon initiation of imbibition. This respiration depended upon cytochrome c oxidase a...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.93.1.295
更新日期:1990-05-01 00:00:00
abstract::Plants are able to respond to herbivore damage with de novo biosynthesis of an herbivore-characteristic blend of volatiles. The signal transduction initiating volatile biosynthesis may involve the activation of the octadecanoid pathway, as exemplified by the transient increase of endogenous jasmonic acid (JA) in leave...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.121.1.153
更新日期:1999-09-01 00:00:00
abstract::Low-oxygen stress imposed by field waterlogging is a serious impediment to plant germination and growth. Plants respond to waterlogging with a complex set of physiological responses regulated at the transcriptional, cellular, and tissue levels. The Arabidopsis (Arabidopsis thaliana) NAC domain-containing gene ANAC102 ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.108.131912
更新日期:2009-04-01 00:00:00
abstract::Cytokinins promote expansion of cotyledons detached from seedlings of more than a dozen species. The zeatin-enhanced expansion of cucumber (Cucumis sativus L. cv Marketer) cotyledons was investigated. In addition, whether acid secretion is involved in wall loosening accompanying such accelerated growth was evaluated. ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.70.5.1470
更新日期:1982-11-01 00:00:00
abstract::Sorghum (Sorghum bicolor L. Moench) accumulates the anthocyanin cyanidin 3-dimalonyl glucoside in etiolated mesocotyls in response to light. Inoculation with the nonpathogenic fungus Cochliobolus heterostrophus drastically reduced the light-induced accumulation of anthocyanin by repressing the transcription of the ant...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.116.3.979
更新日期:1998-03-01 00:00:00
abstract::Purified potato tuber (Solanum tuberosum L. cv Bintie) mitochondria contain soluble, highly latent NAD(+)- and NADP(+)-isocitrate dehydrogenases, NAD(+)- and NADP(+)-malate dehydrogenases, as well as an NADPH-specific glutathione reductase (160, 25, 7200, 160, and 16 nanomoles NAD(P)H per minute and milligram protein,...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.94.3.1012
更新日期:1990-11-01 00:00:00