Stable transformation of ferns using spores as targets: Pteris vittata and Ceratopteris thalictroides.


:Ferns (Pteridophyta) are very important members of the plant kingdom that lag behind other taxa with regards to our understanding of their genetics, genomics, and molecular biology. We report here, to our knowledge, the first instance of stable transformation of fern with recovery of transgenic sporophytes. Spores of the arsenic hyperaccumulating fern Pteris vittata and tetraploid 'C-fern Express' (Ceratopteris thalictroides) were stably transformed by Agrobacterium tumefaciens with constructs containing the P. vittata actin promoter driving a GUSPlus reporter gene. Reporter gene expression assays were performed on multiple tissues and growth stages of gametophytes and sporophytes. Southern-blot analysis confirmed stable transgene integration in recovered sporophytes and also confirmed that no plasmid from A. tumefaciens was present in the sporophyte tissues. We recovered seven independent transformants of P. vittata and four independent C. thalictroides transgenics. Inheritance analyses using β-glucuronidase (GUS) histochemical staining revealed that the GUS transgene was stably expressed in second generation C. thalictroides sporophytic tissues. In an independent experiment, the gusA gene that was driven by the 2× Cauliflower mosaic virus 35S promoter was bombarded into P. vittata spores using biolistics, in which putatively stable transgenic gametophytes were recovered. Transformation procedures required no tissue culture or selectable marker genes. However, we did attempt to use hygromycin selection, which was ineffective for recovering transgenic ferns. This simple stable transformation method should help facilitate functional genomics studies in ferns.


Plant Physiol


Plant physiology


Muthukumar B,Joyce BL,Elless MP,Stewart CN Jr




Has Abstract


2013-10-01 00:00:00














  • A novel image-analysis technique for kinematic study of growth and curvature.

    abstract::Kinematic analysis has provided important insights into the biology of growth by revealing the distribution of expansion within growing organs. Modern methods of kinematic analysis have made use of new image-tracking algorithms and computer-assisted evaluation, but these methods have yet to be adapted for examination ...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Basu P,Pal A,Lynch JP,Brown KM

    更新日期:2007-10-01 00:00:00

  • Photoinduced Carotenogenesis in Chlorotic Euglena gracilis.

    abstract::Light induces beta-carotene synthesis in streptomycin-bleached Euglena gracilis Z. Light-adapted, chemostat-grown cells have up to 10-fold as much beta-carotene and 25% more protein than similarly grown, dark-adapted cells. Carotenogenesis does not occur under anaerobic conditions or in the presence of diphenylamine, ...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Dolphin WD

    更新日期:1970-11-01 00:00:00

  • AtKC1 and CIPK23 Synergistically Modulate AKT1-Mediated Low-Potassium Stress Responses in Arabidopsis.

    abstract::In Arabidopsis (Arabidopsis thaliana), the Shaker K(+) channel AKT1 conducts K(+) uptake in root cells, and its activity is regulated by CBL1/9-CIPK23 complexes as well as by the AtKC1 channel subunit. CIPK23 and AtKC1 are both involved in the AKT1-mediated low-K(+) (LK) response; however, the relationship between the...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Wang XP,Chen LM,Liu WX,Shen LK,Wang FL,Zhou Y,Zhang Z,Wu WH,Wang Y

    更新日期:2016-04-01 00:00:00

  • Amino Acid uptake by pea leaf fragments: specificity, energy sources, and mechanism.

    abstract::Amino acid uptake into leaf fragments of Pisum sativum depended on metabolism. Glycine uptake was optimal at 30 C and could be supported by respiration and by photosynthesis. Based on studies with an electron flow cofactor, inhibitors, and uncouplers, the energy source for glycine uptake was apparently ATP.The energy-...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Cheung YN,Nobel PS

    更新日期:1973-12-01 00:00:00

  • Identification and kinetics of accumulation of proteins induced by ethylene in bean abscission zones.

    abstract::A two-dimensional gel electrophoresis system that combines a cationic polyacrylamide gel electrophoresis at pH near neutrality with sodium dodecyl sulfate-polyacrylamide gel electrophoresis was used to analyze the spectrum of basic polypeptides that accumulate in bean (Phaseolus vulgaris) abscission zones after treatm...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Del Campillo E,Lewis LN

    更新日期:1992-03-01 00:00:00

  • Swelling of Phaseolus mitochondria induced by the action of phospholipase A.

    abstract::Phaseolus vulgaris mitochondria incubated in sucrose swell rapidly upon the addition of phospholipase A. Bovine serum albumin inhibits the swelling. The release of free fatty acids as a result of phospholipase A action on the mitochondria is detected only in the presence of bovine serum albumin, which promotes the hyd...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Earnshaw MJ,Truelove B

    更新日期:1970-03-01 00:00:00

  • A mutation in Arabidopsis seedling plastid development1 affects plastid differentiation in embryo-derived tissues during seedling growth.

    abstract::Oilseed plants like Arabidopsis (Arabidopsis thaliana) develop green photosynthetically active embryos. Upon seed maturation, the embryonic chloroplasts degenerate into a highly reduced plastid type called the eoplast. Upon germination, eoplasts redifferentiate into chloroplasts and other plastid types. Here, we descr...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Ruppel NJ,Logsdon CA,Whippo CW,Inoue K,Hangarter RP

    更新日期:2011-01-01 00:00:00

  • Oxidation of reduced nicotinamide adenine dinucleotide phosphate by isolated corn mitochondria.

    abstract::Isolated corn (Zea mays L.) mitochondria were found to oxidize reduced nicotinamide adenine dinucleotide phosphate in a KCl reaction medium. This oxidation was dependent on the presence of calcium or phosphate or both. Strontium and manganese substituted for calcium, but magnesium or barium did not. The oxidation of N...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Koeppe DE,Miller RJ

    更新日期:1972-03-01 00:00:00

  • Isolation of a Putative Carboxysomal Carbonic Anhydrase Gene from the Cyanobacterium Synechococcus PCC7942.

    abstract::The Type II mutants of the cyanobacterium Synechococcus PCC7942 (G.D. Price, M.R. Badger [1989] Plant Physiol 91: 514-525) are able to accumulate a large pool of inorganic carbon inside the cell, but are unable to utilize it for CO(2) fixation, resulting in a high CO(2)-requiring phenotype. We have isolated a 3.5-kb B...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Yu JW,Price GD,Song L,Badger MR

    更新日期:1992-10-01 00:00:00

  • Genetic and genomic evidence that sucrose is a global regulator of plant responses to phosphate starvation in Arabidopsis.

    abstract::Plants respond to phosphate (Pi) starvation by exhibiting a suite of developmental, biochemical, and physiological changes to cope with this nutritional stress. To understand the molecular mechanism underlying these responses, we isolated an Arabidopsis (Arabidopsis thaliana) mutant, hypersensitive to phosphate starva...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Lei M,Liu Y,Zhang B,Zhao Y,Wang X,Zhou Y,Raghothama KG,Liu D

    更新日期:2011-07-01 00:00:00

  • Increasing sucrose uptake capacity of wheat grains stimulates storage protein synthesis.

    abstract::Increasing grain sink strength by improving assimilate uptake capacity could be a promising approach toward getting higher yield. The barley (Hordeum vulgare) sucrose transporter HvSUT1 (SUT) was expressed under control of the endosperm-specific Hordein B1 promoter (HO). Compared with the wild type, transgenic HOSUT g...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Weichert N,Saalbach I,Weichert H,Kohl S,Erban A,Kopka J,Hause B,Varshney A,Sreenivasulu N,Strickert M,Kumlehn J,Weschke W,Weber H

    更新日期:2010-02-01 00:00:00

  • Chlorophyll Formation and Photosynthetic Competence in Euglena During Light-Induced Chloroplast Development in the Presence of 3, (3,4-dichlorophenyl) 1,1-Dimethyl Urea (DCMU).

    abstract::The possibility that photosynthetic competence is gratuitous for light-induced chloroplast development in Euglena gracilis var. bacillaris was examined by incubating dark-grown resting cells in the light with DCMU, an inhibitor of photosynthesis. Under these conditions photosynthetic carbon dioxide fixation was inhibi...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Schiff JA,Zeldin MH,Rubman J

    更新日期:1967-12-01 00:00:00

  • Measurement of turgor pressure and its gradient in the Phloem of oak.

    abstract::A direct method is described for measuring the pressure in secondary phloem sieve tubes of oak trees. One end of a 26-gauge stainless steel tube was shaped such that when it penetrated the outer bark and transected a few sieve elements, it was stopped by the xylem so that small openings in the end allowed phloem sap t...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Hammel HT

    更新日期:1968-07-01 00:00:00

  • Characteristics of glutamate dehydrogenase in mitochondria prepared from corn shoots.

    abstract::The amination of alpha-ketoglutarate (alpha-KG) by NADH-glutamate dehydrogenase (GDH) obtained from Sephadex G-75 treated crude extracts from shoots of 5-day-old seedlings was stimulated by the addition of Ca(2+). The NADH-GDH purified 161-fold with ammonium sulfate, DEAE-Toyopearl, and Sephadex G-200 was also activat...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Yamaya T,Oaks A,Matsumoto H

    更新日期:1984-12-01 00:00:00

  • Ethylene rapidly up-regulates the activities of both monomeric GTP-binding proteins and protein kinase(s) in epicotyls of pea.

    abstract::It is demonstrated that, in etiolated pea (Pisum sativum) epicotyls, ethylene affects the activation of both monomeric GTP-binding proteins (monomeric G-proteins) and protein kinases. For monomeric G-proteins, the effect may be a rapid (2 min) and bimodal up-regulation, a transiently unimodal activation, or a transien...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Moshkov IE,Novikova GV,Mur LA,Smith AR,Hall MA

    更新日期:2003-04-01 00:00:00

  • Cytokinin-to-Auxin Ratios and Morphology of Shoots and Tissues Transformed by a Chimeric Isopentenyl Transferase Gene.

    abstract::Tissues transformed with the isopentenyl transferase (ipt) gene cloned from the T-DNA region of the Ti plasmid or with the ipt gene placed under the control of the cauliflower mosaic virus 35S promoter (35S-ipt) were analyzed for auxin and cytokinin. Free and total indole-3-acetic acid (IAA) levels in 35S-ipt-transfor...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Smigocki AC,Owens LD

    更新日期:1989-11-01 00:00:00

  • Compromised virus-induced gene silencing in RDR6-deficient plants.

    abstract::RNA silencing in plants serves as a potent antiviral defense mechanism through the action of small interfering RNAs (siRNAs), which direct RNA degradation. siRNAs can be derived directly from the viral genome or via the action of host-encoded RNA-dependent RNA polymerases (RDRs). Plant genomes encode multiple RDRs, an...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Vaistij FE,Jones L

    更新日期:2009-03-01 00:00:00

  • 3,4-Dehydroproline inhibits cell wall assembly and cell division in tobacco protoplasts.

    abstract::We investigated the function of cell wall hydroxyproline-rich glycoproteins by observing the effects of a selective inhibitor of prolyl hydroxylase, 3,4-dehydro-L-proline (Dhp), on wall regeneration by Nicotiana tabacum mesophyll cell protoplasts. Protoplasts treated with micromolar concentrations of Dhp do not develo...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Cooper JB,Heuser JE,Varner JE

    更新日期:1994-02-01 00:00:00

  • Effect of temperature on water and ion transport in soybean and broccoli systems.

    abstract::Steady-state flow rates and exudate osmotic potentials were measured from complete root systems from warm- (28/23 C) or cold-(17/11 C) grown soybean or broccoli (Brassica oleracea) plants at various pressures or different temperatures.In warm-grown soybean roots systems, a break occurred at 14.7 C in the Arrhenius plo...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Markhart AH,Fiscus EL,Naylor AW,Kramer PJ

    更新日期:1979-07-01 00:00:00

  • Activation of a Reserve Pool of Photosystem II in Chlamydomonas reinhardtii Counteracts Photoinhibition.

    abstract::The effect of strong irradiance (2000 micromole photons per square meter per second) on PSII heterogeneity in intact cells of Chlamydomonas reinhardtii was investigated. Low light (LL, 15 micromole photons per square meter per second) grown C. reinhardtii are photoinhibited upon exposure to strong irradiance, and the ...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Neale PJ,Melis A

    更新日期:1990-04-01 00:00:00

  • Manipulation of in vivo sorbitol production alters boron uptake and transport in tobacco

    abstract::Recent evidence that some species can retranslocate boron as complexes with sugar alcohols in the phloem suggests a possible mechanism for enhancing boron efficiency. We investigated the relationship between sugar alcohol (sorbitol) content, boron uptake and distribution, and translocation of foliar-applied, isotopica...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Bellaloui N,Brown PH,Dandekar AM

    更新日期:1999-02-01 00:00:00

  • Complete turgor maintenance at low water potentials in the elongating region of maize leaves.

    abstract::Leaf elongation rate, water potential, and osmotic potential were measured in the fifth leaf of maize (Zea mays L.) plants growing in soil from which water was withheld for varying times. Elongation occurred in the basal region, which was enclosed by other leaf sheaths. When water was withheld from the soil, leaf elon...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Michelena VA,Boyer JS

    更新日期:1982-05-01 00:00:00

  • Abscission: quantitative measurement with a recording abscissor.

    abstract::The construction, operation, and effectiveness of an abscission measuring instrument called an abscissor is described. The device measured the force required for a spring-opposed plunger to shear abscission zone explants and was capable of automatically recording break strength data. Examples of data obtained with the...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Craker LE,Abeles FB

    更新日期:1969-08-01 00:00:00

  • Arabidopsis mitogen-activated protein kinase kinases MKK1 and MKK2 have overlapping functions in defense signaling mediated by MEKK1, MPK4, and MKS1.

    abstract::The Arabidopsis (Arabidopsis thaliana) MKK1 and MKK2 mitogen-activated protein kinase kinases have been implicated in biotic and abiotic stress responses as part of a signaling cascade including MEKK1 and MPK4. Here, the double loss-of-function mutant (mkk1/2) of MKK1 and MKK2 is shown to have marked phenotypes in dev...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Qiu JL,Zhou L,Yun BW,Nielsen HB,Fiil BK,Petersen K,Mackinlay J,Loake GJ,Mundy J,Morris PC

    更新日期:2008-09-01 00:00:00

  • Na-NMR Studies of the Intracellular Sodium Ion Concentration in the Halotolerant Alga Dunaliella salina.

    abstract::The Intracellular Na(+) concentration in the halotolerant alga Dunaliella salina was measured in intact cells by (23)Na-NMR spectroscopy, utilizing the dysprosium tripolyphosphate complex as a sodium shift reagent, and was found to be 88 +/- 28 millimolar. Intracellular sodium ion content and intracellular volume were...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Bental M,Degani H,Avron M

    更新日期:1988-08-01 00:00:00


    abstract::Pectate lyase was isolated from the cell extract of Erwinia aroideae. The enzyme was further purified to a high degree by a procedure involving ammonium sulfate fractionation and chromatography on CM-Sephadex C-50 and on Sephadex G-200. The enzyme attacked its substrate in an endo fashion and was more active on the so...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Konno H,Yamasaki Y

    更新日期:1982-04-01 00:00:00

  • The metabolism of soluble nucleotides in wheat aleurone layers treated with gibberellic Acid.

    abstract::The metabolism of soluble nucleotides was investigated in wheat (Triticum aestivum var. Olympic) aleurone layers treated with gibberellic acid. Whereas nucleotide levels were relatively unaffected by the treatment, a transient increase was observed in the incorporation of (32)P. The effect was maximal 30 to 60 minutes...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Collins GG,Jenner CF,Paleg LG

    更新日期:1972-03-01 00:00:00

  • The structure of chloroplast DNA molecules and the effects of light on the amount of chloroplast DNA during development in Medicago truncatula.

    abstract::We used pulsed-field gel electrophoresis and restriction fragment mapping to analyze the structure of Medicago truncatula chloroplast DNA (cpDNA). We find most cpDNA in genome-sized linear molecules, head-to-tail genomic concatemers, and complex branched forms with ends at defined sites rather than at random sites as ...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Shaver JM,Oldenburg DJ,Bendich AJ

    更新日期:2008-03-01 00:00:00

  • Purification and Characterization of an Endophytic Fungal Proteinase That Is Abundantly Expressed in the Infected Host Grass.

    abstract::A novel Acremonium typhinum proteinase that is expressed during endophytic infection of the grass Poa ampla Merr. was purified from endophyte-infected leaf sheath tissue. It is a thiol-containing serine alkaline endoproteinase with bound carbohydrate. In the infected host tissue, this proteinase is an abundant protein...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Lindstrom JT,Belanger FC

    更新日期:1994-09-01 00:00:00

  • Comparative Transcriptomic Approaches Exploring Contamination Stress Tolerance in Salix sp. Reveal the Importance for a Metaorganismal de Novo Assembly Approach for Nonmodel Plants.

    abstract::Metatranscriptomic study of nonmodel organisms requires strategies that retain the highly resolved genetic information generated from model organisms while allowing for identification of the unexpected. A real-world biological application of phytoremediation, the field growth of 10 Salix cultivars on polluted soils, w...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Brereton NJ,Gonzalez E,Marleau J,Nissim WG,Labrecque M,Joly S,Pitre FE

    更新日期:2016-05-01 00:00:00