Abstract:
:Purified potato tuber (Solanum tuberosum L. cv Bintie) mitochondria contain soluble, highly latent NAD(+)- and NADP(+)-isocitrate dehydrogenases, NAD(+)- and NADP(+)-malate dehydrogenases, as well as an NADPH-specific glutathione reductase (160, 25, 7200, 160, and 16 nanomoles NAD(P)H per minute and milligram protein, respectively). The two isocitrate dehydrogenase activities, but not the two malate dehydrogenase activities, could be separated by ammonium sulfate precipitation. Thus, the NADP(+)-isocitrate dehydrogenase activity is due to a separate matrix enzyme, whereas the NADP(+)-malate dehydrogenase activity is probably due to unspecificity of the NAD(+)-malate dehydrogenase. NADP(+)-specific isocitrate dehydrogenase had much lower K(m)s for NADP(+) and isocitrate (5.1 and 10.7 micromolar, respectively) than the NAD(+)-specific enzyme (101 micromolar for NAD(+) and 184 micromolar for isocitrate). A broad activity optimum at pH 7.4 to 9.0 was found for the NADP(+)-specific isocitrate dehydrogenase whereas the NAD(+)-specific enzyme had a sharp optimum at pH 7.8. Externally added NADP(+) stimulated both isocitrate and malate oxidation by intact mitochondria under conditions where external NADPH oxidation was inhibited. This shows that (a) NADP(+) is taken up by the mitochondria across the inner membrane and into the matrix, and (b) NADP(+)-reducing activities of malate dehydrogenase and the NADP(+)-specific isocitrate dehydrogenase in the matrix can contribute to electron transport in intact plant mitochondria. The physiological relevance of mitochondrial NADP(H) and soluble NADP(H)-consuming enzymes is discussed in relation to other known mitochondrial NADP(H)-utilizing enzymes.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Rasmusson AG,Møller IMdoi
10.1104/pp.94.3.1012subject
Has Abstractpub_date
1990-11-01 00:00:00pages
1012-8issue
3eissn
0032-0889issn
1532-2548journal_volume
94pub_type
杂志文章相关文献
PLANT PHYSIOLOGY文献大全abstract::First leaves and flag leaves of the wheat species Triticum aestivum cv Anza (6x), T. boeoticum Boiss (2x) L. were examined for content of pyruvate, orthophosphate dikinase (PPDK), phosphoenolpyruvate carboxylase (PEPC), and ribulose 1,5-bisphosphate carboxylase (RuBPC) by protein blot analyses using antibodies to maiz...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.82.1.96
更新日期:1986-09-01 00:00:00
abstract::Ethylene inhibited the tuberization of etiolated potato (Solanum tuberosum L. var. Red La Soda) sprout sections cultured in vitro. Carbon dioxide did not overcome the C(2)H(4) inhibition but it was required for normal tuberization. Ethylene totally prevented root formation and development. It inhibited stolon elongati...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.57.4.480
更新日期:1976-04-01 00:00:00
abstract::Gibberellic acid (GA) enhances the synthesis of alpha-amylase and ribonuclease in isolated aleurone layers and this process is inhibited by abscisin. Removal of gibberellic acid in mid-course of alpha-amylase production results in a slowing down of alpha-amylase synthesis, suggesting a continued requirement of GA for ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.42.7.1008
更新日期:1967-07-01 00:00:00
abstract::Phytoene synthase (PSY) catalyzes the highly regulated, frequently rate-limiting synthesis of the first biosynthetically formed carotene. While PSY constitutes a small gene family in most plant taxa, the Brassicaceae, including Arabidopsis (Arabidopsis thaliana), predominantly possess a single PSY gene. This monogenic...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.16.01262
更新日期:2016-12-01 00:00:00
abstract::Removal of the lower epidermis from a tobacco leaf allows a faster and wider range of water fluxes, without damaging the mesophyll. It also permits a more direct examination of the photosynthetic potential of the tissue at various levels of hydration.The rehydration rate of leaf discs is essentially linear. It decreas...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.48.5.575
更新日期:1971-11-01 00:00:00
abstract::Plants have evolved different but interconnected strategies to defend themselves against herbivorous insects and microbial pathogens. We used an Arabidopsis/Pseudomonas syringae pathosystem to investigate the impact of pathogen-induced defense responses on cabbage looper (Trichoplusia ni) larval feeding. Arabidopsis m...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.010815
更新日期:2002-06-01 00:00:00
abstract::Lys deacylases are essential regulators of cell biology in many contexts. Here, we have identified CddA (cyanobacterial deacetylase/depropionylase), a Lys deacylase enzyme expressed in the cyanobacterium Synechococcus sp. PCC 7002 that has both deacetylase and depropionylase activity. Loss of the gene cddA led to slow...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.20.00583
更新日期:2020-10-01 00:00:00
abstract::Root developmental plasticity enables plants to adapt to limiting or fluctuating nutrient conditions in the soil. When grown under nitrogen (N) deficiency, plants develop a more exploratory root system by increasing primary and lateral root length. However, mechanisms underlying this so-called foraging response remain...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.20.00440
更新日期:2020-07-01 00:00:00
abstract::Histone H2B monoubiquitination (H2Bub) is being recognized as a regulatory mechanism that controls a range of cellular processes in plants, but the molecular mechanisms of H2Bub that are involved in responses to biotic stress are largely unknown. In this study, we used wild-type and H2Bub loss-of-function mutations of...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.113.234567
更新日期:2014-04-01 00:00:00
abstract::Three forms of pyrophosphate fructose-6-phosphate 1-phosphotransferase (PFP) were purified from both green and red tomato (Lycopersicon esculentum) fruit: (a) a classical form (designated Q(2)) containing alpha- (66 kilodalton) and beta- (60 kilodalton) subunits; (b) a form (Q(1)) containing a beta-doublet subunit; an...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.94.2.499
更新日期:1990-10-01 00:00:00
abstract::Response regulators of two-component systems play pivotal roles in the transcriptional regulation of responses to environmental signals in bacteria. Rre37, an OmpR-type response regulator, is induced by nitrogen depletion in the unicellular cyanobacterium Synechocystis species PCC 6803. Microarray and quantitative rea...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.113.232025
更新日期:2014-04-01 00:00:00
abstract::Nitrate releases seed dormancy in Arabidopsis (Arabidopsis thaliana) Columbia accession seeds in part by reducing abscisic acid (ABA) levels. Nitrate led to lower levels of ABA in imbibed seeds when included in the germination medium (exogenous nitrate). Nitrate also reduced ABA levels in dry seeds when provided to th...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.108.126938
更新日期:2009-02-01 00:00:00
abstract::The in vivo synthesis of early heat-shock proteins in young leaves of barley (Hordeum vulgare L.) and sorghum (Sorghum bicolor L.) was studied by one- and two-dimensional electrophoresis. Analysis of whole leaf protein patterns demonstrated clearly the enhanced resolution of heat-shock proteins, especially those of lo...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.94.2.567
更新日期:1990-10-01 00:00:00
abstract::The expression of nitrilase in Arabidopsis during the development of the clubroot disease caused by the obligate biotroph Plasmodiophora brassicae was investigated. A time course study showed that only during the exponential growth phase of the clubs was nitrilase prominently enhanced in infected roots compared with c...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.122.2.369
更新日期:2000-02-01 00:00:00
abstract::d-Lactate accumulation in Chlamydomonas reinhardtii was dependent on anaerobic conditions. As much as 50% of the (14)C after 2 minutes of photosynthetic (14)CO(2) fixation moved into d-lactate from sugar phosphates if the cells became anaerobic for short time periods. No lactate accumulated in the dark until the O(2) ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.78.2.277
更新日期:1985-06-01 00:00:00
abstract::Quantitative analysis of statolith sedimentation behavior was accomplished using videomicroscopy of living columella cells of corn (Zea mays) roots, which displayed no systematic cytoplasmic streaming. Following 90 degrees rotation of the root, the statoliths moved downward along the distal wall and then spread out al...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.125.2.1045
更新日期:2001-02-01 00:00:00
abstract::With the aim to specifically study the molecular mechanisms behind photoinhibition of photosystem I, stacked spinach (Spinacia oleracea) thylakoids were irradiated at 4 degrees C with far-red light (>715 nm) exciting photosystem I, but not photosystem II. Selective excitation of photosystem I by far-red light for 130 ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.125.4.2007
更新日期:2001-04-01 00:00:00
abstract::A culture of Chlorella established by 30-fold dilution of a culture already grown to a level of 15 ml packed cell volume per liter produces little chlorophyll for approximately 12 hours. Investigation of other characteristics such as nitrogen incorporation, increase in packed cell volume and dry weight as well as RNA ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.41.10.1695
更新日期:1966-12-01 00:00:00
abstract::THE PRESENT STUDY COMPARES THE BINDING AND INHIBITORY ACTIVITY OF TWO PHOTOSYSTEM II INHIBITORS: 3-(3,4-dichlorophenyl)-1,1-dimethylurea (diuron [DCMU]) and 2-chloro-4-(ethylamine)-6-(isopropyl amine)-S-triazene (atrazine). Chloroplasts isolated from naturally occurring triazine-susceptible and triazine-resistant biot...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.64.6.995
更新日期:1979-12-01 00:00:00
abstract::The ethylene biosynthesis-inducing xylanase (EIX) is known to be a potent elicitor of ethylene biosynthesis and other responses when applied to leaf tissue of Nicotiana tabacum L. cv Xanthi. In contrast, leaf tissue of the tobacco cultivar Hicks was insensitive to EIX at concentrations 100-fold higher than was needed ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.101.3.1081
更新日期:1993-03-01 00:00:00
abstract::A depolarization-activated K(+) channel capable of carrying the large K(+) currents that flow from shrinking cells during movements of Samanea saman leaflets has been described in the plasmalemma of Samanea motor cell protoplasts (N Moran et al [1988] Plant Physiol 88:643-648). We now characterize this channel in grea...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.94.2.424
更新日期:1990-10-01 00:00:00
abstract::Experiments were designed to test the hypothesis that the labeled products recovered from plant tissue incubated with [(14)C]GA(12)-7-aldehyde ([(14)C]GA(12)ald) would serve as appropriate [(14)C]markers for the recovery of naturally-occurring gibberellins (GAs). The [(14)C]GA(12)ald (about 200 millicuries per millimo...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.81.4.984
更新日期:1986-08-01 00:00:00
abstract::Gln is a key player in plant metabolism. It is one of the major free amino acids that is transported into the developing seed and is central for nitrogen metabolism. However, Gln natural variation and its regulation and interaction with other metabolic processes in seeds remain poorly understood. To investigate the la...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.20.00039
更新日期:2020-06-01 00:00:00
abstract::Truncated transcription factor-like proteins called microProteins (miPs) can modulate transcription factor activities, thereby increasing transcriptional regulatory complexity. To understand their prevalence, evolution, and function, we predicted over 400 genes that encode putative miPs from Arabidopsis (Arabidopsis t...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.114.235903
更新日期:2014-05-01 00:00:00
abstract::A cell line of Eschscholtzia californica selected for meta-fluorotyrosine (MFT) tolerance was found to have 10-fold increased levels of phenylalanine and tyrosine compared to the parent line, while most other amino acids were only increased 2-fold. Tracer experiments with shikimic acid in the presence of MFT showed th...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.89.4.1299
更新日期:1989-04-01 00:00:00
abstract::Changes in lipid composition were followed as a proplastid develops into a chloroplast. Methods were devised for the isolation of developing proplastids from sections of five different ages from the same 7-day-old maize (Zea mays var. Kelvedon Glory) leaf. Electron micrographs illustrate the homogeneity of the five ty...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.57.5.789
更新日期:1976-05-01 00:00:00
abstract::Orcinol O-methyltransferase (OOMT) 1 and 2 catalyze the last two steps of the biosynthetic pathway leading to the phenolic methyl ether 3,5-dimethoxytoluene (DMT), the major scent compound of many rose (Rosa x hybrida) varieties. Modern roses are descended from both European and Chinese species, the latter being produ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.105.070961
更新日期:2006-01-01 00:00:00
abstract::Cell-cell and extracellular cell matrix (ECM) interactions provide cells with information essential for controlling morphogenesis, cell-fate specification, and cell death. In animals, one of the major groups of enzymes that degrade the ECM is the matrix metalloproteinases (MMPs). Here, we report the characterization o...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.123.3.917
更新日期:2000-07-01 00:00:00
abstract::We observed the biphasic production of ethylene and reactive oxygen species (ROS) in susceptible tobacco (Nicotiana tabacum 'Wisconsin 38') plants after shoot inoculation with Phytophthora parasitica var nicotianae. The initial transient increase in ROS and ethylene at 1 and 3 h (phase I), respectively, was followed b...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.112.194654
更新日期:2012-05-01 00:00:00
abstract::The effects of ultraviolet-B (UV-B) radiation on stomatal conductance (g(s)) in pea (Pisum sativum L.), commelina (Commelina communis L.), and oilseed rape (Brassica napus L.) plants were investigated. Plants were grown in a greenhouse either with three different high ratios of UV-B to photosynthetically active radiat...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.121.2.489
更新日期:1999-10-01 00:00:00