Expression and localization of nitrilase during symptom development of the clubroot disease in Arabidopsis.

Abstract:

:The expression of nitrilase in Arabidopsis during the development of the clubroot disease caused by the obligate biotroph Plasmodiophora brassicae was investigated. A time course study showed that only during the exponential growth phase of the clubs was nitrilase prominently enhanced in infected roots compared with controls. NIT1 and NIT2 are the nitrilase isoforms predominantly expressed in clubroot tissue, as shown by investigating promoter-beta-glucuronidase fusions of each. Two peaks of beta-glucuronidase activity were visible: an earlier peak (21 d post inoculation) consisting only of the expression of NIT1, and a second peak at about 32 d post inoculation, which predominantly consisted of NIT2 expression. Using a polyclonal antibody against nitrilase, it was shown that the protein was mainly found in infected cells containing sporulating plasmodia, whereas in cells of healthy roots and in uninfected cells of inoculated roots only a few immunosignals were detected. To determine which effect a missing nitrilase isoform might have on symptom development, the P. brassicae infection in a nitrilase mutant (nit1-3) of Arabidopsis was investigated. As a comparison, transgenic plants overexpressing NIT2 under the control of the cauliflower mosaic virus 35S promoter were studied. Root galls were smaller in nit1-3 plants compared with the wild type. The phenotype of smaller clubs in the mutant was correlated with a lower free indole-3-acetic acid content in the clubs compared with the wild type. Overexpression of nitrilase did not result in larger clubs compared with the wild type. The putative role of nitrilase and auxins during symptom development is discussed.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Grsic-Rausch S,Kobelt P,Siemens JM,Bischoff M,Ludwig-Müller J

doi

10.1104/pp.122.2.369

subject

Has Abstract

pub_date

2000-02-01 00:00:00

pages

369-78

issue

2

eissn

0032-0889

issn

1532-2548

journal_volume

122

pub_type

杂志文章
  • Regulation of Photosynthetic Rate of Two Sunflower Hybrids under Water Stress.

    abstract::The effect of short-term water stress on photosynthesis of two sunflower hybrids (Helianthus annuus L. cv Sungro-380 and cv SH-3622), differing in productivity under field conditions, was measured. The rate of CO(2) assimilation of young, mature leaves of SH-3622 under well-watered conditions was approximately 30% gre...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.98.2.516

    authors: Gimenez C,Mitchell VJ,Lawlor DW

    更新日期:1992-02-01 00:00:00

  • Polygalacturonase Isozymes and Pectin Depolymerization in Transgenic rin Tomato Fruit.

    abstract::We have previously described the construction and expression of a chimeric gene that allows developmentally regulated expression of tomato (Lycopersicon esculentum) polygalacturonase in ripening-impaired, mutant (rin) tomato fruit (JJ Giovannoni, D DellaPenna, AB Bennett, RL Fischer [1989] The Plant Cell 1: 53-63). We...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.94.4.1882

    authors: Dellapenna D,Lashbrook CC,Toenjes K,Giovannoni JJ,Fischer RL,Bennett AB

    更新日期:1990-12-01 00:00:00

  • Changes in Protein Patterns and Translatable Messenger RNA Populations during Cold Acclimation of Alfalfa.

    abstract::Changes in the rate and pattern of protein synthesis and in translatable mRNA population during cold acclimation of alfalfa (Medicago falcata cv Anik) seedlings have been examined. There appears to be a positive correlation between the increase in ability to synthesize proteins at 4 degrees C and the increase in freez...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.84.4.1172

    authors: Mohapatra SS,Poole RJ,Dhindsa RS

    更新日期:1987-08-01 00:00:00

  • The Anti-nptII Gene (A Potential Negative Selectable Marker for Plants).

    abstract::An efficient negative selection procedure is crucial to the isolation of rare homologous recombinants in gene targeting. Although gene targeting is a common practice in lower eukaryotes and is becoming routine in mammals, its application to plants has not been achieved. In this report, we have evaluated an antisense c...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.102.1.287

    authors: Xiang C,Guerra DJ

    更新日期:1993-05-01 00:00:00

  • Effects of inorganic phosphate on the light dependent thylakoid energization of intact spinach chloroplasts.

    abstract::The light dependent energization of the thylakoid membrane was analyzed in isolated intact spinach (Spinacia oleracea L.) chloroplasts incubated with different concentrations of inorganic phosphate (Pi). Two independent methods were used: (a) the accumulation of [(14)C]5,5-dimethyl-2,4-oxazolidinedione and [(14)C] met...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.91.1.221

    authors: Heineke D,Stitt M,Heldt HW

    更新日期:1989-09-01 00:00:00

  • The abscisic acid induction of a novel peroxidase is antagonized by cytokinin in Spirodela polyrrhiza L.

    abstract::The growth regulator abscisic acid (ABA) can be used to induce dormant bud structures (turions) in the duckweed Spirodela polyrrhiza L. In this paper we show that during this process, ABA rapidly induces elevated levels of mRNA transcripts encoding a novel basic peroxidase. In addition, we show that in the presence of...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.105.2.497

    authors: Chaloupková K,Smart CC

    更新日期:1994-06-01 00:00:00

  • Comparative cross-species alternative splicing in plants.

    abstract::Alternative splicing (AS) can add significantly to genome complexity. Plants are thought to exhibit less AS than animals. An algorithm, based on expressed sequence tag (EST) pairs gapped alignment, was developed that takes advantage of the relatively small intron and exon size in plants and directly compares pairs of ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.107.098640

    authors: Ner-Gaon H,Leviatan N,Rubin E,Fluhr R

    更新日期:2007-07-01 00:00:00

  • Expression of the Heat Shock Response in a Tomato Interspecific Hybrid Is Not Intermediate between the Two Parental Responses.

    abstract::While it is apparent that the heat shock response is ubiquitous, variabilities in the nature of the heat shock response between closely related species have not been well characterized. The heat shock response of three genotypes of tomato, Lycopersicon esculentum, Lycopersicon pennellii, and the interspecific sexual h...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.93.3.1140

    authors: Fender SE,O'connell MA

    更新日期:1990-07-01 00:00:00

  • Kinetic Variance of Ribulose-1,5-bisphosphate Carboxylase/Oxygenase Isolated from Diverse Taxonomic Sources : II. Analysis by Two Dual Label Methods.

    abstract::Two dual label methods were used to investigate kinetic variability of ribulose 1,5-bisphosphate (RuBP) carboxylase/oxygenase (EC 4.1.1.39). In addition to using [1-(14)C,5-(3)H]RuBP (method 1), we describe here the detailed assay with (14)CO(2) and [5-(3)H]RuBP (method 2), which generates [(3)H,(14)C]3-phosphoglyceri...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.75.3.645

    authors: Kent SS,Tomany MJ

    更新日期:1984-07-01 00:00:00

  • Auxin and red light in the control of hypocotyl hook opening in beans.

    abstract::Evidence is presented to support the suggestion that endogenous auxinlike substances participate in controlling the unbending of the hypocotyl hook of Phaseolus vulgaris L. (cv. Black Valentine). An acidic indole was detected in hook diffusates by fluorometry; triiodobenzoic acid, an inhibitor of auxin transport, prev...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.48.2.187

    authors: Rubinstein B

    更新日期:1971-08-01 00:00:00

  • Oxygen Concentration in Isolated Chloroplasts during Photosynthesis.

    abstract::The O(2) concentration in intact and osmotically disrupted isolated spinach (Spinacia oleracea, L.) chloroplasts during photosynthesis was estimated. The chloroplasts were allowed to reduce 3-phosphoglycerate, CO(2), or ferricyanide in light until the rate of O(2) production was linear. When the light was turned off O...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.60.6.903

    authors: Steiger HM,Beck E

    更新日期:1977-12-01 00:00:00

  • Degree of C(4) Photosynthesis in C(4) and C(3)-C(4)Flaveria Species and Their Hybrids : II. Inhibition of Apparent Photosynthesis by a Phosphoenolpyruvate Carboxylase Inhibitor.

    abstract::Hybrids have been made between species of Flaveria exhibiting varying levels of C(4) photosynthesis. The degree of C(4) photosynthesis expressed in four interspecific hybrids (Flaveria trinervia [C(4)] x F. linearis [C(3)-C(4)], F. brownii [C(4)-like] x F. linearis, and two three-species hybrids from F. trinervia x [F...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.100.2.947

    authors: Brown RH,Byrd GT,Black CC

    更新日期:1992-10-01 00:00:00

  • Water-stress-induced ethylene production in wheat : a fact or artifact?

    abstract::Effects of water stress on ethylene evolution from excised leaf segments and intact plants of wheat (Triticum aestivum L. cv Katepwa) were studied. Excised leaf segments of 8 day or 6 week old plants were dried until they lost 8% of their fresh weight (water potential about -2.3 megapascals). These and nondried contro...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.96.2.406

    authors: Narayana I,Lalonde S,Saini HS

    更新日期:1991-06-01 00:00:00

  • Amide-Linked Indoleacetic Acid Conjugates May Control Levels of Indoleacetic Acid in Germinating Seedlings of Phaseolus vulgaris.

    abstract::We have shown that amide-linked IAA (indole-3-acetic acid) conjugates accumulated to high levels during maturation of bean seeds (K. Bialek and J.D. Cohen [1989] Plant Physiol 91: 775-779). In the present study, we were interested in the fate of these and other IAA conjugates during seed germination. The content of am...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.100.4.2002

    authors: Bialek K,Cohen JD

    更新日期:1992-12-01 00:00:00

  • The AP2/ERF domain transcription factor ORA59 integrates jasmonic acid and ethylene signals in plant defense.

    abstract::Plant defense against pathogens depends on the action of several endogenously produced hormones, including jasmonic acid (JA) and ethylene. In certain defense responses, JA and ethylene signaling pathways synergize to activate a specific set of defense genes. Here, we describe the role of the Arabidopsis (Arabidopsis ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.108.117523

    authors: Pré M,Atallah M,Champion A,De Vos M,Pieterse CM,Memelink J

    更新日期:2008-07-01 00:00:00

  • Circadian rhythmicity in excised samanea pulvini: I. Sucrose-white light interactions.

    abstract::The rhythmic movement of excised Samanea saman pulvini incubated in H(2)O or 50 mm sucrose was monitored during extended periods of white light (cool white fluorescent, 2,000 ft-c), darkness, or alternating white light (16 hr) and darkness (8 hr). In continuous white light, the rhythm damps at an intermediate angle af...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.58.3.417

    authors: Simon E,Satter RL,Galston AW

    更新日期:1976-09-01 00:00:00

  • The dominant mutant Wavy auricle in blade1 disrupts patterning in a lateral domain of the maize leaf.

    abstract::Mature maize leaves have defined cell types along the proximal distal and medial lateral axes. The patterning events that establish these axes take place early in leaf initiation. We have identified a new dominant mutation, Wavy auricle in blade1 (Wab1), which affects patterning in both axes in a dose-dependent manner...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.103.036707

    authors: Hay A,Hake S

    更新日期:2004-05-01 00:00:00

  • Characteristics of glutamate dehydrogenase in mitochondria prepared from corn shoots.

    abstract::The amination of alpha-ketoglutarate (alpha-KG) by NADH-glutamate dehydrogenase (GDH) obtained from Sephadex G-75 treated crude extracts from shoots of 5-day-old seedlings was stimulated by the addition of Ca(2+). The NADH-GDH purified 161-fold with ammonium sulfate, DEAE-Toyopearl, and Sephadex G-200 was also activat...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.76.4.1009

    authors: Yamaya T,Oaks A,Matsumoto H

    更新日期:1984-12-01 00:00:00

  • The G-Box Transcriptional Regulatory Code in Arabidopsis.

    abstract::Plants have significantly more transcription factor (TF) families than animals and fungi, and plant TF families tend to contain more genes; these expansions are linked to adaptation to environmental stressors. Many TF family members bind to similar or identical sequence motifs, such as G-boxes (CACGTG), so it is diffi...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.17.01086

    authors: Ezer D,Shepherd SJK,Brestovitsky A,Dickinson P,Cortijo S,Charoensawan V,Box MS,Biswas S,Jaeger KE,Wigge PA

    更新日期:2017-10-01 00:00:00

  • β-galactosyl Yariv reagent binds to the β-1,3-galactan of arabinogalactan proteins.

    abstract::Yariv phenylglycosides [1,3,5-tri(p-glycosyloxyphenylazo)-2,4,6-trihydroxybenzene] are a group of chemical compounds that selectively bind to arabinogalactan proteins (AGPs), a type of plant proteoglycan. Yariv phenylglycosides are widely used as cytochemical reagents to perturb the molecular functions of AGPs as well...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.112.211722

    authors: Kitazawa K,Tryfona T,Yoshimi Y,Hayashi Y,Kawauchi S,Antonov L,Tanaka H,Takahashi T,Kaneko S,Dupree P,Tsumuraya Y,Kotake T

    更新日期:2013-03-01 00:00:00

  • Detecting mutants that have impaired photosynthesis by their increased level of fluorescence.

    abstract::A technique is described for detecting mutants of Chlamydomonas reinhardi that have impaired photosynthesis. It is based on the fact that when photosynthetic electron transport is blocked there may be an increase in the level of fluorescence similar to that obtained in the presence of an inhibitor of photosynthetic el...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.42.9.1284

    authors: Bennoun P,Levine RP

    更新日期:1967-09-01 00:00:00

  • The Effect of Nitrogen Nutrition on the Cellular Localization of Glutamine Synthetase Isoforms in Barley Roots.

    abstract::Glutamine synthetase (GS) was detected by immunogold localization in the cytosol and plastids of roots of 7-d-old barley (Hordeum vulgare L. cv Klaxon) seedlings grown in the presence or absence of NO3- (15 mM) or NH4+ (30 mM). The number of GS polypeptides changed during root development, and this was affected by N n...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.111.4.1109

    authors: Peat LJ,Tobin AK

    更新日期:1996-08-01 00:00:00

  • Malate Metabolism in Leaf Mitochondria from the Crassulacean Acid Metabolism Plant Kalanchoë blossfeldiana Poelln.

    abstract::The mechanisms and the controlling factors of malate oxidation by mitochondria from leaves of Kalanchoë blossfeldiana Poelln. plants performing Crassulacean acid metabolism were investigated using Percollpurified mitochondria. The effects of pH and of various cofactors (ATP, NAD(+), coenzyme A) on malate dehydrogenase...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.81.4.1039

    authors: Rustin P,Lance C

    更新日期:1986-08-01 00:00:00

  • Partial purification of gibberellin oxidases from spinach leaves.

    abstract::Four enzyme activities catalyzing the following oxidative steps in the gibberellin (GA) biosynthetic pathway have been extracted from spinach (Spinacia oleracea L.) leaves after exposure to 8 long days: GA(12) --> GA(53) --> GA(44) --> GA(19) --> GA(20). Two of these, GA(53) oxidase and GA(19) oxidase, were separable ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.85.1.87

    authors: Gilmour SJ,Bleecker AB,Zeevaart JA

    更新日期:1987-09-01 00:00:00

  • Role of Potassium in Carbon Dioxide Assimilation in Medicago sativa L.

    abstract::Alfalfa was grown hydroponically in 0, 0.6, and 4.8 millimolar K in order to determine the influence of tissue level of K on photosynthesis, dark respiration, photorespiration, stomatal and mesophyll resistance to CO(2), photosystem I and II activity, and synthesis and activity of ribulose 1,5-bisphosphate carboxylase...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.63.5.878

    authors: Peoples TR,Koch DW

    更新日期:1979-05-01 00:00:00

  • Periplasmic carbonic anhydrase structural gene (Cah1) mutant in chlamydomonas reinhardtii

    abstract::To survive in various conditions of CO2 availability, Chlamydomonas reinhardtii shows adaptive changes, such as induction of a CO2-concentrating mechanism, changes in cell organization, and induction of several genes, including a periplasmic carbonic anhydrase (pCA1) encoded by Cah1. Among a collection of insertionall...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.120.3.757

    authors: Van K,Spalding MH

    更新日期:1999-07-01 00:00:00

  • A class I ADP-ribosylation factor GTPase-activating protein is critical for maintaining directional root hair growth in Arabidopsis.

    abstract::Membrane trafficking and cytoskeletal dynamics are important cellular processes that drive tip growth in root hairs. These processes interact with a multitude of signaling pathways that allow for the efficient transfer of information to specify the direction in which tip growth occurs. Here, we show that AGD1, a class...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.108.119529

    authors: Yoo CM,Wen J,Motes CM,Sparks JA,Blancaflor EB

    更新日期:2008-08-01 00:00:00

  • An aquaglyceroporin is abundantly expressed early in the development of the suspensor and the embryo proper of loblolly pine.

    abstract::In contrast to angiosperms, pines and other gymnosperms form well-developed suspensors in somatic embryogenic cultures. This creates a useful system to study suspensor biology. In a study of gene expression during the early stages of conifer embryogenesis, we identified a transcript, PtNIP1;1, that is abundant in imma...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:

    authors: Ciavatta VT,Morillon R,Pullman GS,Chrispeels MJ,Cairney J

    更新日期:2001-12-01 00:00:00

  • Abscisic acid regulates axillary bud outgrowth responses to the ratio of red to far-red light.

    abstract::Low red light/far-red light ratio (R:FR) serves as an indicator of impending competition and has been demonstrated to suppress branch development. The regulation of Arabidopsis (Arabidopsis thaliana) rosette bud outgrowth by the R:FR and the associated mechanisms were investigated at several levels. Growth under low R...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.113.221895

    authors: Reddy SK,Holalu SV,Casal JJ,Finlayson SA

    更新日期:2013-10-01 00:00:00

  • Biochemical characterization of a spearmint mutant that resembles peppermint in monoterpene content.

    abstract::A radiation-induced mutant of Scotch spearmint (Mentha x gracilis) was shown to produce an essential oil containing principally C3-oxygenated p-menthane monoterpenes that are typical of peppermint, instead of the C6-oxygenated monoterpene family characteristic of spearmint. In vitro measurement of all of the enzymes r...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.96.3.744

    authors: Croteau R,Karp F,Wagschal KC,Satterwhite DM,Hyatt DC,Skotland CB

    更新日期:1991-07-01 00:00:00