Abstract:
:We have previously described the construction and expression of a chimeric gene that allows developmentally regulated expression of tomato (Lycopersicon esculentum) polygalacturonase in ripening-impaired, mutant (rin) tomato fruit (JJ Giovannoni, D DellaPenna, AB Bennett, RL Fischer [1989] The Plant Cell 1: 53-63). We now show that expression of the chimeric polygalacturonase gene in rin tomato fruit resulted in the accumulation of all three polygalacturonase isozymes (PG1, PG2A, and PG2B). Polyuronide solubilization and polyuronide depolymerization both reached their maximal levels in transgenic rin fruit prior to the appearance of PG2 isozymes. These results demonstrate that PG1, PG2A, and PG2B all arise by differential processing of a single gene product and further suggest that the PG1 isozyme is sufficient to carry out both polyuronide solubilization and depolymerization in vivo.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Dellapenna D,Lashbrook CC,Toenjes K,Giovannoni JJ,Fischer RL,Bennett ABdoi
10.1104/pp.94.4.1882subject
Has Abstractpub_date
1990-12-01 00:00:00pages
1882-6issue
4eissn
0032-0889issn
1532-2548journal_volume
94pub_type
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