Abstract:
:Membrane trafficking and cytoskeletal dynamics are important cellular processes that drive tip growth in root hairs. These processes interact with a multitude of signaling pathways that allow for the efficient transfer of information to specify the direction in which tip growth occurs. Here, we show that AGD1, a class I ADP ribosylation factor GTPase-activating protein, is important for maintaining straight growth in Arabidopsis (Arabidopsis thaliana) root hairs, since mutations in the AGD1 gene resulted in wavy root hair growth. Live cell imaging of growing agd1 root hairs revealed bundles of endoplasmic microtubules and actin filaments extending into the extreme tip. The wavy phenotype and pattern of cytoskeletal distribution in root hairs of agd1 partially resembled that of mutants in an armadillo repeat-containing kinesin (ARK1). Root hairs of double agd1 ark1 mutants were more severely deformed compared with single mutants. Organelle trafficking as revealed by a fluorescent Golgi marker was slightly inhibited, and Golgi stacks frequently protruded into the extreme root hair apex of agd1 mutants. Transient expression of green fluorescent protein-AGD1 in tobacco (Nicotiana tabacum) epidermal cells labeled punctate bodies that partially colocalized with the endocytic marker FM4-64, while ARK1-yellow fluorescent protein associated with microtubules. Brefeldin A rescued the phenotype of agd1, indicating that the altered activity of an AGD1-dependent ADP ribosylation factor contributes to the defective growth, organelle trafficking, and cytoskeletal organization of agd1 root hairs. We propose that AGD1, a regulator of membrane trafficking, and ARK1, a microtubule motor, are components of converging signaling pathways that affect cytoskeletal organization to specify growth orientation in Arabidopsis root hairs.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Yoo CM,Wen J,Motes CM,Sparks JA,Blancaflor EBdoi
10.1104/pp.108.119529subject
Has Abstractpub_date
2008-08-01 00:00:00pages
1659-74issue
4eissn
0032-0889issn
1532-2548pii
pp.108.119529journal_volume
147pub_type
杂志文章相关文献
PLANT PHYSIOLOGY文献大全abstract::Subsequent production of amylase is severely inhibited if barban [4-chloro-2-butynyl N-(3-chlorophenyl) carbamate] is added to embryo-free half seeds of barley within 4 to 5 hours after gibberellic acid treatment of these seeds. Thirty to 50 mg/L concentrations of barban are effective. Barban inhibition is non-competi...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.42.2.195
更新日期:1967-02-01 00:00:00
abstract::Wheat (Triticum aestivum L. cv Etoile de Choisy) microsomes catalyzed the cytochrome P-450-dependent oxidation of the herbicide diclofop to three hydroxy-diclofop isomers. Hydroxylation was predominant at carbon 4, with migration of chlorine to carbon 5 (67%) and carbon 3 (25%). The 2,4-dichloro-5-hydroxy isomer was i...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.100.2.874
更新日期:1992-10-01 00:00:00
abstract::Ferns (Pteridophyta) are very important members of the plant kingdom that lag behind other taxa with regards to our understanding of their genetics, genomics, and molecular biology. We report here, to our knowledge, the first instance of stable transformation of fern with recovery of transgenic sporophytes. Spores of ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.113.224675
更新日期:2013-10-01 00:00:00
abstract::The pbs3-1 mutant, identified in a screen for Arabidopsis (Arabidopsis thaliana) mutants exhibiting enhanced susceptibility to the avirulent Pseudomonas syringae pathogen DC3000 (avrPphB), also exhibits enhanced susceptibility to virulent P. syringae strains, suggesting it may impact basal disease resistance. Because ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.107.097691
更新日期:2007-06-01 00:00:00
abstract::The apparent association of ornithine decarboxylase (ODC) with rapid cell proliferation in developing tomato (Lycopersicon esculentum Mill. cv. Pearson ms-35) fruits has been previously described. Further evidence is provided by the use of two ODC inhibitors, alpha-difluoromethylornithine (alpha-DFMO) and alpha-methyl...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.70.2.540
更新日期:1982-08-01 00:00:00
abstract::Spinach (Spinacia oleracea CV Bloomsdale Long Standing) leaf cytoplasmic starch phosphorylase and rabbit muscle phosphorylase a were inactivated by incubation with partially purified leaf extract in the presence of ATP and Mg(2+). The inactivating factor(s) were heat stable and susceptible to protease attack. Phosphor...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.73.4.902
更新日期:1983-12-01 00:00:00
abstract::The identity and scope of chloroplast and mitochondrial proteases in higher plants has only started to become apparent in recent years. Biochemical and molecular studies suggested the existence of Clp, FtsH, and DegP proteases in chloroplasts, and a Lon protease in mitochondria, although currently the full extent of t...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.125.4.1912
更新日期:2001-04-01 00:00:00
abstract::Exposure of dark grown resting Euglena to ethanol produced a transient increase in the specific activity of the glyoxysomal enzyme malate synthase. Enzyme specific activity increased during the first 24 hours of ethanol treatment and then declined. Light exposure or malate addition failed to increase enzyme specific a...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.68.2.430
更新日期:1981-08-01 00:00:00
abstract::Extracellular calmodulin (ExtCaM) exerts multiple functions in animals and plants, but the mode of ExtCaM action is not well understood. In this paper, we provide evidence that ExtCaM stimulates a cascade of intracellular signaling events to regulate stomatal movement. Analysis of the changes of cytosolic free Ca2+ ([...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.104.047837
更新日期:2004-12-01 00:00:00
abstract::Storage compound accumulation during seed development prepares the next generation of plants for survival. Therefore, processes involved in the regulation and synthesis of storage compound accumulation during seed development bear relevance to germination and seedling establishment. The wrinkled1 (wri1) mutant of Arab...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.106.079574
更新日期:2006-06-01 00:00:00
abstract::We have isolated the plasma membrane H+-ATPase in a phosphorylated form from spinach (Spinacia oleracea L.) leaf tissue incubated with fusicoccin, a fungal toxin that induces irreversible binding of 14-3-3 protein to the C terminus of the H+-ATPase, thus activating H+ pumping. We have identified threonine-948, the sec...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.118.2.551
更新日期:1998-10-01 00:00:00
abstract::Two hydroxamic acids isolated from maize extracts, 2,4-dihydroxy-7-methoxy-1,4-(2H)-benzoxazin-3(4H)-one (DIMBOA) and the 2-O-beta-d-glucopyranoside of DIMBOA, inhibit photophosphorylation by spinach chloroplasts. Both cyclic and noncyclic photophosphorylations were inhibited to the same extent. The concentrations pro...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.68.4.941
更新日期:1981-10-01 00:00:00
abstract::Numerous studies have demonstrated a rapid increase in the respiration rate during aging of slices of tuber and storage roots. To determine the molecular mechanisms of this phenomenon, the role of enzyme binding to the subcellular particulate fraction has been assessed in carrot (Daucus carota L.) and sugar beet (Beta...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.86.2.348
更新日期:1988-02-01 00:00:00
abstract::Measurements were made of (45)Ca influx into isolated internodal cells of Chara corallina and also into internodal cells of intact plants. (45)Ca influx was closely related to growth. In rapidly expanding internodal cells, the influx was approximately 1.4 nmol m(-2) s(-1) compared to the influx in mature cells from sl...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.100.2.637
更新日期:1992-10-01 00:00:00
abstract::A two-dimensional gel electrophoresis system that combines a cationic polyacrylamide gel electrophoresis at pH near neutrality with sodium dodecyl sulfate-polyacrylamide gel electrophoresis was used to analyze the spectrum of basic polypeptides that accumulate in bean (Phaseolus vulgaris) abscission zones after treatm...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.98.3.955
更新日期:1992-03-01 00:00:00
abstract::Isolated cells obtained by enzymic digestion of young primary leaves of cold-hardened, dark-grown Kharkov winter wheat (Triticum aestivum L.) were exposed to various low temperature stresses. The initial uptake of (86)Rb was generally decreased by increasing concentrations of Ca(2+), but after longer periods of incuba...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.78.3.484
更新日期:1985-07-01 00:00:00
abstract::The tissue distributions of dhurrin [p-hydroxy-(S)-mandelonitrile-beta-d-glucoside] and of enzymes involved in its metabolism have been investigated in leaf blades of light-grown Sorghum bicolor seedlings. Enzymic digestion of these leaves using cellulase has enabled preparations of epidermal and mesophyll protoplasts...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.63.6.1022
更新日期:1979-06-01 00:00:00
abstract::Wheat (Triticum aestivum L. cv Albis) was grown in open-top chambers in the field and fumigated daily with charcoal-filtered air (0.015 microliters per liter O(3)), nonfiltered air (0.03 microliters per liter O(3)), and air enriched with either 0.07 or 0.10 microliters per liter ozone (seasonal 8 hour/day [9 am-5 pm] ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.88.4.1115
更新日期:1988-12-01 00:00:00
abstract::Exposure of six wheat (Triticum aestivum L.) and one rye (Secale cereale L.) cultivar to 40% relative humidity for 24 hours induced the same degree of freezing tolerance in seedling epicotyls as did cold conditioning for 4 weeks at 2 degrees C.Frost hardiness varietal relationships were the same in desiccation-stresse...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.69.1.256
更新日期:1982-01-01 00:00:00
abstract::Bound auxin metabolism in cultured crown-gall tumor cells and pith callus of tobacco was examined by feeding radiolabeled auxins and auxin conjugates. In all tissues fed [(14)C]indoleacetic acid (IAA), at least one-third of the IAA was decarboxylated, and most of the remaining radiolabel occurred in a compound(s) whic...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.80.2.315
更新日期:1986-02-01 00:00:00
abstract::The EGG CELL1 (EC1) gene family of Arabidopsis (Arabidopsis thaliana) comprises five members that are specifically expressed in the egg cell and redundantly control gamete fusion during double fertilization. We investigated the activity of all five EC1 promoters in promoter-deletion studies and identified SUF4 (SUPPRE...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.16.01024
更新日期:2017-01-01 00:00:00
abstract::Three novel beta-xylan xylanohydrolases capable of dissociating ferulated arabinoxylan (Feraxan) from maize (Zea mays L. hybrid B73 x Mo17) coleoptile sections and two conventional beta-xylan xylanohydrolases (xylanases) were purified from a Bacillus subtilis industrial enzyme preparation (Novo Ban L-120). The Feraxan...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.87.4.883
更新日期:1988-08-01 00:00:00
abstract::In pea stem segments whose cuticle has been made permeable by abrading it, actinomycin D (ActD) and 80S ribosomal protein synthesis inhibitors such as cycloheximide (CHI) inhibit enhancement by indole 3-acetic acid (IAA) of the activity of the cell wall biosynthetic enzyme, glucan synthase I (GS). This supersedes earl...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.85.2.523
更新日期:1987-10-01 00:00:00
abstract::The properties of oxaloacetate (OA) transport into mitochondria from potato (Solanum tuberosum) tuber and pea (Pisum sativum) leaves were studied by measuring the uptake of 14C-labeled OA into liposomes with incorporated mitochondrial membrane proteins preloaded with various dicarboxylates or citrate. OA was found to ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.119.3.1025
更新日期:1999-03-01 00:00:00
abstract::Changes in acyl lipids and pigments during leaf development in a virescens barley mutant (M) and the normal (N) were studied. Apical 3-cm leaf segments were extracted with chloroform-methanol, the extracts were purified on Sephadex G-25 columns, and the polar lipids were separated on two-dimensional-thin layer chromat...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.67.4.646
更新日期:1981-04-01 00:00:00
abstract::Elongation of intact young leaves of maize was found to be dynamically dependent on soil water supply. With adequate water, elongation was remarkably constant but slowed when the water potential of the soil in pots dropped from -0.1 to -0.2 bar and stopped when it dropped to -2.5 bars. The corresponding range of leaf ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.48.5.631
更新日期:1971-11-01 00:00:00
abstract::Serial analysis of gene expression was used to profile transcript levels in Arabidopsis roots and assess their responses to 2,4,6-trinitrotoluene (TNT) exposure. SAGE libraries representing control and TNT-exposed seedling root transcripts were constructed, and each was sequenced to a depth of roughly 32,000 tags. Mor...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.103.028019
更新日期:2003-11-01 00:00:00
abstract::Although cytokinins (CKs) affect a number of processes connected with chloroplasts, it has never been rigorously proven that chloroplasts contain CKs. We isolated intact chloroplasts from tobacco (Nicotiana tabacum L. cv SR1) and wheat (Triticum aestivum L. cv Ritmo) leaves and determined their CKs by liquid chromatog...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.121.1.245
更新日期:1999-09-01 00:00:00
abstract::The kinetics of (14)CO(2) carboxylation and decarboxylation in corn root tips were determined to ascertain the sequence of product formation and subsequent utilization, and to obtain further evidence to predict the enzymes mediating the carboxylation and decarboxylations. The carboxylation data indicated that the firs...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.42.5.712
更新日期:1967-05-01 00:00:00
abstract::Purified ribulose-1,5-bisphosphate carboxylase/oxygenase in 50% saturated (NH(4))(2)SO(4) was stable when frozen as small beads in liquid nitrogen and stored at -80 C. When stored as a slurry at 4 C most of the activity was lost within four weeks. This loss was due not only to enzyme polymerization. Activity in old pr...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.67.6.1220
更新日期:1981-06-01 00:00:00