Binding of glycolytic enzymes to a particulate fraction in carrot and sugar beet storage roots : dependence on metabolic state.

Abstract:

:Numerous studies have demonstrated a rapid increase in the respiration rate during aging of slices of tuber and storage roots. To determine the molecular mechanisms of this phenomenon, the role of enzyme binding to the subcellular particulate fraction has been assessed in carrot (Daucus carota L.) and sugar beet (Beta vulgaris L.). Soluble versus particulate fractions were separated by centrifugation at 16,000g and both fractions assayed for the activities of six glycolytic enzymes. Preparations from sliced and aged tissues showed elevated percentages of five enzymes associated with the particulate fraction as compared with controls. The stimulation of respiration which occurs during aging of underground storage organ slices may result, in part, from an association of enzymes with the particulate fraction of the cell promoting an elevated glycolytic rate.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Moorhead GB,Plaxton WC

doi

10.1104/pp.86.2.348

subject

Has Abstract

pub_date

1988-02-01 00:00:00

pages

348-51

issue

2

eissn

0032-0889

issn

1532-2548

journal_volume

86

pub_type

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