Abstract:
:Changes in the rate and pattern of protein synthesis and in translatable mRNA population during cold acclimation of alfalfa (Medicago falcata cv Anik) seedlings have been examined. There appears to be a positive correlation between the increase in ability to synthesize proteins at 4 degrees C and the increase in freezing resistance (survival at -10 degrees C). Results obtained with three different approaches using sodium dodecyl sulfate-polyacrylamide gel electrophoresis pattern visualized by (a) staining, (b) immunoblotting and autoradiography, and (c) fluorography of in vivo labeled proteins, show that at least eight polypeptides are newly synthesized during cold acclimation. Results of analysis of in vitro translation products of mRNA from nonacclimated and acclimated seedlings show the appearance of new translatable mRNAs. It is concluded that changes in gene expression occur during cold acclimation, most probably at the transcriptional level.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Mohapatra SS,Poole RJ,Dhindsa RSdoi
10.1104/pp.84.4.1172subject
Has Abstractpub_date
1987-08-01 00:00:00pages
1172-6issue
4eissn
0032-0889issn
1532-2548journal_volume
84pub_type
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