Abstract:
:Pear fruit cells (Pyrus communis L. cv Passe Crassane) stopped dividing when subcultured in a bioreactor under auxin starvation in the presence of 0.37 molar mannitol. The cessation of cell division was preceded by the accumulation of a specific basic polypeptide of 24 kilodalton. Readdition of 2.3 micromolar 2,4-dichlorophenoxyacetic acid (2,4-D) neither caused a resumption of cell division nor depressed the accumulation of this polypeptide. Under complete auxin starvation, cells began to die at day 18. In vivo radioactive labeling of proteins followed by two-dimensional electrophoresis showed that during auxin starvation the synthesis of some polypeptides including the 24 kilodalton one (referred to as homeostasis-related proteins, HRPs) was decreased while the synthesis of some others (referred as senescence-related proteins, SRPs) was increased. Readdition of 2.3 micromolar 2,4-D postponed the onset of cell death by 10 to 15 days while supplementation with 7.6 micromolar abscisic acid advanced cell death by 8 days. Two-dimensional analysis of protein synthesis indicated that both hormones interact on the synthesis of these two groups of polypeptides. The levels of most HRPs were maintained or increased in the presence of auxin, while the levels of the SRPs were decreased by auxin and increased by abscisic acid. Short and long-term effects of 2,4-D and abscisic acid on the synthesis of specific polypeptides were observed, allowing a discrimination between the direct and indirect effect of both hormones on the development of cell senescence.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Lelievre JM,Balague C,Pech JC,Meyer Ydoi
10.1104/pp.85.2.400subject
Has Abstractpub_date
1987-10-01 00:00:00pages
400-6issue
2eissn
0032-0889issn
1532-2548journal_volume
85pub_type
杂志文章相关文献
PLANT PHYSIOLOGY文献大全abstract::Two mutations, amylose-extender and waxy, which affect the proportion of amylose and amylopectin of starch synthesized in the endosperm of maize (Zea mays L.) seeds, are also expressed in the pollen. However, most mutations that affect starch synthesis in the maize endosperm are not expressed in the pollen. In an atte...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.63.2.312
更新日期:1979-02-01 00:00:00
abstract::A beta-d-glucanase highly specific for glucans containing a linkage sequence ... Glc 1 --> 4 Glc 1 --> 3 Glc 1 --> 4 Glc ... has been isolated from several commercial preparations of Bacillus subtilis alpha-amylase including one purified by repeated crystallization. The beta-d-glucanase will not hydrolyze cellulose or...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.60.2.300
更新日期:1977-08-01 00:00:00
abstract::The assimilation of NH(4) (+) causes a rapid increase in respiration to provided carbon skeletons for amino acid synthesis. In this study we propose a model for the regulation of carbon partitioning from starch to respiration and N assimilation in the green alga Selenastrum minutum. We provide evidence for both a cyto...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.93.1.166
更新日期:1990-05-01 00:00:00
abstract::The major light-harvesting chlorophyll a/b binding protein (LHCP) of higher plant chloroplasts is nuclear-encoded, synthesized as a precursor, and processed upon import. We have previously (GK Lamppa, M Abad [1987] J Cell Biol 105: 2641-2648) identified a soluble enzyme that cleaves the LHCP precursor (pLHCP). In this...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.90.1.117
更新日期:1989-05-01 00:00:00
abstract::Computing techniques are used to simulate the course of uptake of K(+), Na(+), and Cl(-) by low-salt roots. Measurements of the fluxes of these ions in high-salt roots are used to calculate membrane permeabilities, which are then used to calculate cell uptake. In this way it is possible to test the predictive value of...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.44.10.1417
更新日期:1969-10-01 00:00:00
abstract::A method was developed where soybean seedlings were grown without roots to study the influence of hormones of root origin on shoot growth. Excision of the root resulted in inhibition of apical section growth and DNA synthesis and inhibited elongating section growth. A synthetic cytokinin restored DNA synthesis in the ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.44.9.1295
更新日期:1969-09-01 00:00:00
abstract::Several polyclonal sera were raised in rabbits and in mice against putative sucrose carrier proteins, i.e. a 42 kilodalton (O Gallet, R Lemoine, C Larsson, S Delrot [1989] Biochim Biophys Acta 978: 56-64) and a 62 kD (KG Ripp, PV Viitanen, WD Hitz, VR Fransceschi [1988] Plant Physiol 88: 1435-1445) polypeptide of the ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.98.1.17
更新日期:1992-01-01 00:00:00
abstract::Pectate lyase was isolated from the cell extract of Erwinia aroideae. The enzyme was further purified to a high degree by a procedure involving ammonium sulfate fractionation and chromatography on CM-Sephadex C-50 and on Sephadex G-200. The enzyme attacked its substrate in an endo fashion and was more active on the so...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.69.4.864
更新日期:1982-04-01 00:00:00
abstract::Tyrosine decarboxylase (TYDC) is a common plant enzyme involved in the biosynthesis of numerous secondary metabolites, including hydroxycinnamic acid amides. Although a definite function has not yet been determined, amides have been proposed to form a physical barrier against pathogens because they are usually found a...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.120.3.653
更新日期:1999-07-01 00:00:00
abstract::Cell-cell and extracellular cell matrix (ECM) interactions provide cells with information essential for controlling morphogenesis, cell-fate specification, and cell death. In animals, one of the major groups of enzymes that degrade the ECM is the matrix metalloproteinases (MMPs). Here, we report the characterization o...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.123.3.917
更新日期:2000-07-01 00:00:00
abstract::The Intracellular Na(+) concentration in the halotolerant alga Dunaliella salina was measured in intact cells by (23)Na-NMR spectroscopy, utilizing the dysprosium tripolyphosphate complex as a sodium shift reagent, and was found to be 88 +/- 28 millimolar. Intracellular sodium ion content and intracellular volume were...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.87.4.813
更新日期:1988-08-01 00:00:00
abstract::The glyoxysomal enzymes isocitrate lyase and catalase have been isolated from etiolated cucumber (Cucumis sativus) cotyledons. The enzymes co-purified through polyethyleneimine precipitation and (NH(4))(2)SO(4) precipitation, and were resolved by gel filtration on Sepharose 6B followed by chromatography on diethylamin...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.62.5.754
更新日期:1978-11-01 00:00:00
abstract::Foliar applications of 2 milligrams per liter of 2-chloro-4,6-bis (ethylamino)-s-triazine, 2-methylmercapto-4-ethylamino-6-isobutylamino-s-triazine, and 2-methoxy-4-isopropylamino-6-butylamino-s-triazine caused increases in the activities of starch phosphorylase, pyruvate kinase, cytochrome oxidase, and glutamate dehy...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.48.5.517
更新日期:1971-11-01 00:00:00
abstract::Cuticular waxes are complex mixtures of very-long-chain fatty acids (VLCFAs) and their derivatives, forming a natural barrier on aerial surfaces of terrestrial plants against biotic and abiotic stresses. In VLCFA biosynthesis, β-ketoacyl-CoA synthase (KCS) is the key enzyme, catalyzing the first reaction in fatty acid...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.16.01527
更新日期:2017-02-01 00:00:00
abstract::The conversion of l-glutamate to delta-aminolevulinate, in preparations of cucumber etiochloroplasts incubated in vitro, was inhibited by protoheme IX and Mg-protoporphyrin IX. Mg-protoporphyrin IX was destroyed in the presence of air and plastids; this breakdown was accelerated by S-adenosyl methionine. Mg-protoporph...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.69.1.112
更新日期:1982-01-01 00:00:00
abstract::Phytophthora megasperma Drechs. f. sp. glycinea Kuan & Erwin (PMG) cell wall glucan has been extensively characterized as an elicitor of the pterocarpan phytoalexins, the glyceollins in soybean (Glycine max L.). Just recently, this glucan was shown to be a potent elicitor of conjugates of the isoflavones, daidzein and...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.97.4.1445
更新日期:1991-12-01 00:00:00
abstract::OsbZIP46 is one member of the third subfamily of bZIP transcription factors in rice (Oryza sativa). It has high sequence similarity to ABA-responsive element binding factor (ABF/AREB) transcription factors ABI5 and OsbZIP23, two transcriptional activators positively regulating stress tolerance in Arabidopsis (Arabidop...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.111.190389
更新日期:2012-04-01 00:00:00
abstract::The aim of this study was to characterize the effect of decreased 2-cysteine peroxiredoxin (2-CP) on the leaf anti-oxidative system in Arabidopsis. At three stages of leaf development, two lines of transgenic Arabidopsis mutants with decreased contents of chloroplast 2-CP were compared with wild type and a control lin...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.124.2.823
更新日期:2000-10-01 00:00:00
abstract::Air pollutants are known to cause visible leaf injury as well as impairment of photosynthetic CO(2) fixation. Here we evaluate whether the effects on photosynthesis are large enough to cause changes in the relative composition of stable carbon isotopes, delta(13)C, of plant tissue samples, and, if so, how the changes ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.88.1.218
更新日期:1988-09-01 00:00:00
abstract::Salicylic acid (SA) plays a central role in plant disease resistance, and emerging evidence indicates that auxin, an essential plant hormone in regulating plant growth and development, is involved in plant disease susceptibility. GH3.5, a member of the GH3 family of early auxin-responsive genes in Arabidopsis (Arabido...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.107.106021
更新日期:2007-10-01 00:00:00
abstract::Localization of mRNAs at the subcellular level is an essential mechanism for specific protein targeting and local control of protein synthesis in both eukaryotes and bacteria. While mRNA localization is well documented in metazoans, somatic cells, and microorganisms, only a handful of well-defined mRNA localization ex...
journal_title:Plant physiology
pub_type: 杂志文章,评审
doi:10.1104/pp.19.00972
更新日期:2020-01-01 00:00:00
abstract::The anatomy of strawberry (Fragaria x ananassa) fruit, in which the achene is found on the outer part of the fruit, makes it an excellent species for studying the regulation of fruit development. It can provide a model for the cross talk between primary and secondary metabolism, whose role is of pivotal importance in ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.108.120691
更新日期:2008-10-01 00:00:00
abstract::The plant-specific translation initiation complex eIFiso4F is encoded by three genes in Arabidopsis (Arabidopsis thaliana)-genes encoding the cap binding protein eIFiso4E (eifiso4e) and two isoforms of the large subunit scaffolding protein eIFiso4G (i4g1 and i4g2). To quantitate phenotypic changes, a phenomics platfor...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.19.00557
更新日期:2019-09-01 00:00:00
abstract::The growth potential of embryonic axes of Acer saccharum Marsh. increased during moist storage at 5 C but not at 20 C. During the period of increasing growth potential, the oxygen consumption of the axes remained constant. It was possible to distinguish three phases of the stratification-germination process at 5 C wit...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.53.1.91
更新日期:1974-01-01 00:00:00
abstract::To establish infection, pathogens deliver effectors into host cells to target immune signaling components, including elements of mitogen-activated protein kinase (MPK) cascades. The virulence function of AvrRpt2, one of the first identified Pseudomonas syringae effectors, involves cleavage of the plant defense regulat...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.16.00336
更新日期:2016-07-01 00:00:00
abstract::Surface glandular trichomes distributed throughout the aerial parts of sweet basil (Ocimum basilicum) produce and store monoterpene, sesquiterpene, and phenylpropene volatiles. Three distinct basil chemotypes were used to examine the molecular mechanisms underlying the divergence in their monoterpene and sesquiterpene...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.104.051318
更新日期:2004-11-01 00:00:00
abstract::Magnesium (Mg) chelatase is a heterotrimeric enzyme complex that catalyzes a key regulatory and enzymatic reaction in chlorophyll biosynthesis, the insertion of Mg(2+) into protoporphyrin IX. Studies of the enzyme complex reconstituted in vitro have shown that all three of its subunits, CHL I, CHL D, and CHL H, are re...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.010625
更新日期:2002-02-01 00:00:00
abstract::In the interaction between Arabidopsis (Arabidopsis thaliana) and the generalist herbivorous insect Spodoptera littoralis, little is known about early events in defense signaling and their link to downstream phytohormone pathways. S. littoralis oral secretions induced both Ca²⁺ and phytohormone elevation in Arabidopsi...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.112.198150
更新日期:2012-07-01 00:00:00
abstract::In maize (Zea mays) and other grasses, changes in orientation of stems are perceived by pulvinal tissue, which responds to the stimulus by differential growth resulting in upward bending of the stem. The amyloplast-containing bundle sheath cells are the sites of gravity perception, although the initial steps of gravit...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.127.1.119
更新日期:2001-09-01 00:00:00
abstract::The effects of ultraviolet-B (UV-B) radiation on stomatal conductance (g(s)) in pea (Pisum sativum L.), commelina (Commelina communis L.), and oilseed rape (Brassica napus L.) plants were investigated. Plants were grown in a greenhouse either with three different high ratios of UV-B to photosynthetically active radiat...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.121.2.489
更新日期:1999-10-01 00:00:00