Protein synthesis associated with quiescence and senescence in auxin-starved pear cells.

Abstract:

:Pear fruit cells (Pyrus communis L. cv Passe Crassane) stopped dividing when subcultured in a bioreactor under auxin starvation in the presence of 0.37 molar mannitol. The cessation of cell division was preceded by the accumulation of a specific basic polypeptide of 24 kilodalton. Readdition of 2.3 micromolar 2,4-dichlorophenoxyacetic acid (2,4-D) neither caused a resumption of cell division nor depressed the accumulation of this polypeptide. Under complete auxin starvation, cells began to die at day 18. In vivo radioactive labeling of proteins followed by two-dimensional electrophoresis showed that during auxin starvation the synthesis of some polypeptides including the 24 kilodalton one (referred to as homeostasis-related proteins, HRPs) was decreased while the synthesis of some others (referred as senescence-related proteins, SRPs) was increased. Readdition of 2.3 micromolar 2,4-D postponed the onset of cell death by 10 to 15 days while supplementation with 7.6 micromolar abscisic acid advanced cell death by 8 days. Two-dimensional analysis of protein synthesis indicated that both hormones interact on the synthesis of these two groups of polypeptides. The levels of most HRPs were maintained or increased in the presence of auxin, while the levels of the SRPs were decreased by auxin and increased by abscisic acid. Short and long-term effects of 2,4-D and abscisic acid on the synthesis of specific polypeptides were observed, allowing a discrimination between the direct and indirect effect of both hormones on the development of cell senescence.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Lelievre JM,Balague C,Pech JC,Meyer Y

doi

10.1104/pp.85.2.400

subject

Has Abstract

pub_date

1987-10-01 00:00:00

pages

400-6

issue

2

eissn

0032-0889

issn

1532-2548

journal_volume

85

pub_type

杂志文章
  • Starch-synthesizing Enzymes in the Endosperm and Pollen of Maize.

    abstract::Two mutations, amylose-extender and waxy, which affect the proportion of amylose and amylopectin of starch synthesized in the endosperm of maize (Zea mays L.) seeds, are also expressed in the pollen. However, most mutations that affect starch synthesis in the maize endosperm are not expressed in the pollen. In an atte...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.63.2.312

    authors: Bryce WH,Nelson OE

    更新日期:1979-02-01 00:00:00

  • Preparation and Properties of a beta-d-Glucanase for the Specific Hydrolysis of beta-d-Glucans.

    abstract::A beta-d-glucanase highly specific for glucans containing a linkage sequence ... Glc 1 --> 4 Glc 1 --> 3 Glc 1 --> 4 Glc ... has been isolated from several commercial preparations of Bacillus subtilis alpha-amylase including one purified by repeated crystallization. The beta-d-glucanase will not hydrolyze cellulose or...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.60.2.300

    authors: Huber DJ,Nevins DJ

    更新日期:1977-08-01 00:00:00

  • Regulation of Carbon Partitioning to Respiration during Dark Ammonium Assimilation by the Green Alga Selenastrum minutum.

    abstract::The assimilation of NH(4) (+) causes a rapid increase in respiration to provided carbon skeletons for amino acid synthesis. In this study we propose a model for the regulation of carbon partitioning from starch to respiration and N assimilation in the green alga Selenastrum minutum. We provide evidence for both a cyto...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.93.1.166

    authors: Turpin DH,Botha FC,Smith RG,Feil R,Horsey AK,Vanlerberghe GC

    更新日期:1990-05-01 00:00:00

  • Properties of a Chloroplast Enzyme that Cleaves the Chlorophyll a/b Binding Protein Precursor : Optimization of an Organelle-Free Reaction.

    abstract::The major light-harvesting chlorophyll a/b binding protein (LHCP) of higher plant chloroplasts is nuclear-encoded, synthesized as a precursor, and processed upon import. We have previously (GK Lamppa, M Abad [1987] J Cell Biol 105: 2641-2648) identified a soluble enzyme that cleaves the LHCP precursor (pLHCP). In this...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.90.1.117

    authors: Abad MS,Clark SE,Lamppa GK

    更新日期:1989-05-01 00:00:00

  • Simulation of Cl Uptake by Low-salt Barley Roots as a Test of Models of Salt Uptake.

    abstract::Computing techniques are used to simulate the course of uptake of K(+), Na(+), and Cl(-) by low-salt roots. Measurements of the fluxes of these ions in high-salt roots are used to calculate membrane permeabilities, which are then used to calculate cell uptake. In this way it is possible to test the predictive value of...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.44.10.1417

    authors: Pitman MG

    更新日期:1969-10-01 00:00:00

  • Hormonal regulation of cell elongation in the hypocotyl of rootless soybean: an evaluation of the role of DNA synthesis.

    abstract::A method was developed where soybean seedlings were grown without roots to study the influence of hormones of root origin on shoot growth. Excision of the root resulted in inhibition of apical section growth and DNA synthesis and inhibited elongating section growth. A synthetic cytokinin restored DNA synthesis in the ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.44.9.1295

    authors: Holm RE,Key JL

    更新日期:1969-09-01 00:00:00

  • Selective Inhibition of Active Uptake of Sucrose into Plasma Membrane Vesicles by Polyclonal Sera Directed against a 42 Kilodalton Plasma Membrane Polypeptide.

    abstract::Several polyclonal sera were raised in rabbits and in mice against putative sucrose carrier proteins, i.e. a 42 kilodalton (O Gallet, R Lemoine, C Larsson, S Delrot [1989] Biochim Biophys Acta 978: 56-64) and a 62 kD (KG Ripp, PV Viitanen, WD Hitz, VR Fransceschi [1988] Plant Physiol 88: 1435-1445) polypeptide of the ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.98.1.17

    authors: Gallet O,Lemoine R,Gaillard C,Larsson C,Delrot S

    更新日期:1992-01-01 00:00:00

  • Studies on the Pectic Substances of Plant Cell Walls: III. DEGRADATION OF CARROT ROOT CELL WALLS BY ENDOPECTATE LYASE PURIFIED FROM ERWINIA AROIDEAE.

    abstract::Pectate lyase was isolated from the cell extract of Erwinia aroideae. The enzyme was further purified to a high degree by a procedure involving ammonium sulfate fractionation and chromatography on CM-Sephadex C-50 and on Sephadex G-200. The enzyme attacked its substrate in an endo fashion and was more active on the so...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.69.4.864

    authors: Konno H,Yamasaki Y

    更新日期:1982-04-01 00:00:00

  • Decreased cell wall digestibility in canola transformed with chimeric tyrosine decarboxylase genes from opium poppy

    abstract::Tyrosine decarboxylase (TYDC) is a common plant enzyme involved in the biosynthesis of numerous secondary metabolites, including hydroxycinnamic acid amides. Although a definite function has not yet been determined, amides have been proposed to form a physical barrier against pathogens because they are usually found a...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.120.3.653

    authors: Facchini PJ,Yu M,Penzes-Yost C

    更新日期:1999-07-01 00:00:00

  • A matrix metalloproteinase gene is expressed at the boundary of senescence and programmed cell death in cucumber.

    abstract::Cell-cell and extracellular cell matrix (ECM) interactions provide cells with information essential for controlling morphogenesis, cell-fate specification, and cell death. In animals, one of the major groups of enzymes that degrade the ECM is the matrix metalloproteinases (MMPs). Here, we report the characterization o...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.123.3.917

    authors: Delorme VG,McCabe PF,Kim DJ,Leaver CJ

    更新日期:2000-07-01 00:00:00

  • Na-NMR Studies of the Intracellular Sodium Ion Concentration in the Halotolerant Alga Dunaliella salina.

    abstract::The Intracellular Na(+) concentration in the halotolerant alga Dunaliella salina was measured in intact cells by (23)Na-NMR spectroscopy, utilizing the dysprosium tripolyphosphate complex as a sodium shift reagent, and was found to be 88 +/- 28 millimolar. Intracellular sodium ion content and intracellular volume were...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.87.4.813

    authors: Bental M,Degani H,Avron M

    更新日期:1988-08-01 00:00:00

  • Regulation of Glyoxysomal Enzymes during Germination of Cucumber: 2. Isolation and Immunological Detection of Isocitrate Lyase and Catalase.

    abstract::The glyoxysomal enzymes isocitrate lyase and catalase have been isolated from etiolated cucumber (Cucumis sativus) cotyledons. The enzymes co-purified through polyethyleneimine precipitation and (NH(4))(2)SO(4) precipitation, and were resolved by gel filtration on Sepharose 6B followed by chromatography on diethylamin...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.62.5.754

    authors: Lamb JE,Riezman H,Becker WM

    更新日期:1978-11-01 00:00:00

  • Influence of s-Triazines on Some Enzymes of Carbohydrates and Nitrogen Metabolism in Leaves of Pea (Pisum sativum L.) and Sweet Corn (Zea mays L.).

    abstract::Foliar applications of 2 milligrams per liter of 2-chloro-4,6-bis (ethylamino)-s-triazine, 2-methylmercapto-4-ethylamino-6-isobutylamino-s-triazine, and 2-methoxy-4-isopropylamino-6-butylamino-s-triazine caused increases in the activities of starch phosphorylase, pyruvate kinase, cytochrome oxidase, and glutamate dehy...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.48.5.517

    authors: Wu MT,Singh B,Salunkhe DK

    更新日期:1971-11-01 00:00:00

  • A β-Ketoacyl-CoA Synthase Is Involved in Rice Leaf Cuticular Wax Synthesis and Requires a CER2-LIKE Protein as a Cofactor.

    abstract::Cuticular waxes are complex mixtures of very-long-chain fatty acids (VLCFAs) and their derivatives, forming a natural barrier on aerial surfaces of terrestrial plants against biotic and abiotic stresses. In VLCFA biosynthesis, β-ketoacyl-CoA synthase (KCS) is the key enzyme, catalyzing the first reaction in fatty acid...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.16.01527

    authors: Wang X,Guan Y,Zhang D,Dong X,Tian L,Qu LQ

    更新日期:2017-02-01 00:00:00

  • Effects of Iron and Oxygen on Chlorophyll Biosynthesis : II. OBSERVATIONS ON THE BIOSYNTHETIC PATHWAY IN ISOLATED ETIOCHLOROPLASTS.

    abstract::The conversion of l-glutamate to delta-aminolevulinate, in preparations of cucumber etiochloroplasts incubated in vitro, was inhibited by protoheme IX and Mg-protoporphyrin IX. Mg-protoporphyrin IX was destroyed in the presence of air and plastids; this breakdown was accelerated by S-adenosyl methionine. Mg-protoporph...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.69.1.112

    authors: Chereskin BM,Castelfranco PA

    更新日期:1982-01-01 00:00:00

  • Rapid Accumulation of Anionic Peroxidases and Phenolic Polymers in Soybean Cotyledon Tissues following Treatment with Phytophthora megasperma f. sp. Glycinea Wall Glucan.

    abstract::Phytophthora megasperma Drechs. f. sp. glycinea Kuan & Erwin (PMG) cell wall glucan has been extensively characterized as an elicitor of the pterocarpan phytoalexins, the glyceollins in soybean (Glycine max L.). Just recently, this glucan was shown to be a potent elicitor of conjugates of the isoflavones, daidzein and...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.97.4.1445

    authors: Graham MY,Graham TL

    更新日期:1991-12-01 00:00:00

  • Constitutive activation of transcription factor OsbZIP46 improves drought tolerance in rice.

    abstract::OsbZIP46 is one member of the third subfamily of bZIP transcription factors in rice (Oryza sativa). It has high sequence similarity to ABA-responsive element binding factor (ABF/AREB) transcription factors ABI5 and OsbZIP23, two transcriptional activators positively regulating stress tolerance in Arabidopsis (Arabidop...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.111.190389

    authors: Tang N,Zhang H,Li X,Xiao J,Xiong L

    更新日期:2012-04-01 00:00:00

  • Antisense suppression of 2-cysteine peroxiredoxin in Arabidopsis specifically enhances the activities and expression of enzymes associated with ascorbate metabolism but not glutathione metabolism.

    abstract::The aim of this study was to characterize the effect of decreased 2-cysteine peroxiredoxin (2-CP) on the leaf anti-oxidative system in Arabidopsis. At three stages of leaf development, two lines of transgenic Arabidopsis mutants with decreased contents of chloroplast 2-CP were compared with wild type and a control lin...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.124.2.823

    authors: Baier M,Noctor G,Foyer CH,Dietz KJ

    更新日期:2000-10-01 00:00:00

  • Effects of Air Pollutants on the Composition of Stable Carbon Isotopes, deltaC, of Leaves and Wood, and on Leaf Injury.

    abstract::Air pollutants are known to cause visible leaf injury as well as impairment of photosynthetic CO(2) fixation. Here we evaluate whether the effects on photosynthesis are large enough to cause changes in the relative composition of stable carbon isotopes, delta(13)C, of plant tissue samples, and, if so, how the changes ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.88.1.218

    authors: Martin B,Bytnerowicz A,Thorstenson YR

    更新日期:1988-09-01 00:00:00

  • Dual regulation role of GH3.5 in salicylic acid and auxin signaling during Arabidopsis-Pseudomonas syringae interaction.

    abstract::Salicylic acid (SA) plays a central role in plant disease resistance, and emerging evidence indicates that auxin, an essential plant hormone in regulating plant growth and development, is involved in plant disease susceptibility. GH3.5, a member of the GH3 family of early auxin-responsive genes in Arabidopsis (Arabido...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.107.106021

    authors: Zhang Z,Li Q,Li Z,Staswick PE,Wang M,Zhu Y,He Z

    更新日期:2007-10-01 00:00:00

  • mRNA Localization in Plant Cells.

    abstract::Localization of mRNAs at the subcellular level is an essential mechanism for specific protein targeting and local control of protein synthesis in both eukaryotes and bacteria. While mRNA localization is well documented in metazoans, somatic cells, and microorganisms, only a handful of well-defined mRNA localization ex...

    journal_title:Plant physiology

    pub_type: 杂志文章,评审

    doi:10.1104/pp.19.00972

    authors: Tian L,Chou HL,Fukuda M,Kumamaru T,Okita TW

    更新日期:2020-01-01 00:00:00

  • Reconfiguration of the achene and receptacle metabolic networks during strawberry fruit development.

    abstract::The anatomy of strawberry (Fragaria x ananassa) fruit, in which the achene is found on the outer part of the fruit, makes it an excellent species for studying the regulation of fruit development. It can provide a model for the cross talk between primary and secondary metabolism, whose role is of pivotal importance in ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.108.120691

    authors: Fait A,Hanhineva K,Beleggia R,Dai N,Rogachev I,Nikiforova VJ,Fernie AR,Aharoni A

    更新日期:2008-10-01 00:00:00

  • eIFiso4G Augments the Synthesis of Specific Plant Proteins Involved in Normal Chloroplast Function.

    abstract::The plant-specific translation initiation complex eIFiso4F is encoded by three genes in Arabidopsis (Arabidopsis thaliana)-genes encoding the cap binding protein eIFiso4E (eifiso4e) and two isoforms of the large subunit scaffolding protein eIFiso4G (i4g1 and i4g2). To quantitate phenotypic changes, a phenomics platfor...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.19.00557

    authors: Lellis AD,Patrick RM,Mayberry LK,Lorence A,Campbell ZC,Roose JL,Frankel LK,Bricker TM,Hellmann HA,Mayberry RW,Zavala AS,Choy GS,Wylie DC,Abdul-Moheeth M,Masood A,Prater AG,Van Hoorn HE,Cole NA,Browning KS

    更新日期:2019-09-01 00:00:00

  • High Energy Charge as a Requirement for Axis Elongation in Response to Gibberellic Acid and Kinetin during Stratification of Acer saccharum Seeds.

    abstract::The growth potential of embryonic axes of Acer saccharum Marsh. increased during moist storage at 5 C but not at 20 C. During the period of increasing growth potential, the oxygen consumption of the axes remained constant. It was possible to distinguish three phases of the stratification-germination process at 5 C wit...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.53.1.91

    authors: Simmonds JA,Dumbroff EB

    更新日期:1974-01-01 00:00:00

  • Bacterial AvrRpt2-Like Cysteine Proteases Block Activation of the Arabidopsis Mitogen-Activated Protein Kinases, MPK4 and MPK11.

    abstract::To establish infection, pathogens deliver effectors into host cells to target immune signaling components, including elements of mitogen-activated protein kinase (MPK) cascades. The virulence function of AvrRpt2, one of the first identified Pseudomonas syringae effectors, involves cleavage of the plant defense regulat...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.16.00336

    authors: Eschen-Lippold L,Jiang X,Elmore JM,Mackey D,Shan L,Coaker G,Scheel D,Lee J

    更新日期:2016-07-01 00:00:00

  • The biochemical and molecular basis for the divergent patterns in the biosynthesis of terpenes and phenylpropenes in the peltate glands of three cultivars of basil.

    abstract::Surface glandular trichomes distributed throughout the aerial parts of sweet basil (Ocimum basilicum) produce and store monoterpene, sesquiterpene, and phenylpropene volatiles. Three distinct basil chemotypes were used to examine the molecular mechanisms underlying the divergence in their monoterpene and sesquiterpene...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.104.051318

    authors: Iijima Y,Davidovich-Rikanati R,Fridman E,Gang DR,Bar E,Lewinsohn E,Pichersky E

    更新日期:2004-11-01 00:00:00

  • Chlorophyll biosynthesis. Expression of a second chl I gene of magnesium chelatase in Arabidopsis supports only limited chlorophyll synthesis.

    abstract::Magnesium (Mg) chelatase is a heterotrimeric enzyme complex that catalyzes a key regulatory and enzymatic reaction in chlorophyll biosynthesis, the insertion of Mg(2+) into protoporphyrin IX. Studies of the enzyme complex reconstituted in vitro have shown that all three of its subunits, CHL I, CHL D, and CHL H, are re...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.010625

    authors: Rissler HM,Collakova E,DellaPenna D,Whelan J,Pogson BJ

    更新日期:2002-02-01 00:00:00

  • CML42-mediated calcium signaling coordinates responses to Spodoptera herbivory and abiotic stresses in Arabidopsis.

    abstract::In the interaction between Arabidopsis (Arabidopsis thaliana) and the generalist herbivorous insect Spodoptera littoralis, little is known about early events in defense signaling and their link to downstream phytohormone pathways. S. littoralis oral secretions induced both Ca²⁺ and phytohormone elevation in Arabidopsi...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.112.198150

    authors: Vadassery J,Reichelt M,Hause B,Gershenzon J,Boland W,Mithöfer A

    更新日期:2012-07-01 00:00:00

  • Cytoplasmic pH dynamics in maize pulvinal cells induced by gravity vector changes.

    abstract::In maize (Zea mays) and other grasses, changes in orientation of stems are perceived by pulvinal tissue, which responds to the stimulus by differential growth resulting in upward bending of the stem. The amyloplast-containing bundle sheath cells are the sites of gravity perception, although the initial steps of gravit...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.127.1.119

    authors: Johannes E,Collings DA,Rink JC,Allen NS

    更新日期:2001-09-01 00:00:00

  • Characterization of stomatal closure caused by ultraviolet-B radiation

    abstract::The effects of ultraviolet-B (UV-B) radiation on stomatal conductance (g(s)) in pea (Pisum sativum L.), commelina (Commelina communis L.), and oilseed rape (Brassica napus L.) plants were investigated. Plants were grown in a greenhouse either with three different high ratios of UV-B to photosynthetically active radiat...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.121.2.489

    authors: Nogues S,Allen DJ,Morison JI,Baker NR

    更新日期:1999-10-01 00:00:00