Regulation of Glyoxysomal Enzymes during Germination of Cucumber: 2. Isolation and Immunological Detection of Isocitrate Lyase and Catalase.

Abstract:

:The glyoxysomal enzymes isocitrate lyase and catalase have been isolated from etiolated cucumber (Cucumis sativus) cotyledons. The enzymes co-purified through polyethyleneimine precipitation and (NH(4))(2)SO(4) precipitation, and were resolved by gel filtration on Sepharose 6B followed by chromatography on diethylaminoethyl-cellulose (isocitrate lyase) or hydroxylapatite (catalase). Purity of the isolated enzymes was assessed by sodium dodecyl sulfate-polyacrylamide electrophoresis, isoelectric focusing, and immunoelectrophoresis. Antibodies raised to both enzymes in rabbits and in tumor-bearing mice were shown to be monospecific by immunoelectrophoresis against total homogenate protein. Isocitrate lyase and catalase represent about 0.56% and 0.1%, respectively, of total extractable cotyledonary protein. Both enzymes appear to be present in a single form. Molecular weights of the native enzymes and its subunits are 225,000 and 54,500 for catalase, and 325,000 and 63,500 for isocitrate lyase. The pH optimum for isocitrate lyase is about 6.75 in morpholinopropane sulfonic acid buffer, but varies significantly with buffer used. The K(m) for d-isocitrate is 39 micromolar. A double antibody technique (rabbit anti-isocitrate lyase followed by (125)I-labeled goat anti-rabbit immunoglobulin G) has been used to visualize isocitrate lyase subunit protein on sodium dodecyl sulfate-polyacrylamide with high specificity and sensitivity.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Lamb JE,Riezman H,Becker WM

doi

10.1104/pp.62.5.754

subject

Has Abstract

pub_date

1978-11-01 00:00:00

pages

754-60

issue

5

eissn

0032-0889

issn

1532-2548

journal_volume

62

pub_type

杂志文章
  • Tracing cationic nutrients from xylem into stem tissue of French bean by stable isotope tracers and cryo-secondary ion mass spectrometry.

    abstract::Fluxes of mineral nutrients in the xylem are strongly influenced by interactions with the surrounding stem tissues and are probably regulated by them. Toward a mechanistic understanding of these interactions, we applied stable isotope tracers of magnesium, potassium, and calcium continuously to the transpiration strea...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.109.143776

    authors: Metzner R,Schneider HU,Breuer U,Thorpe MR,Schurr U,Schroeder WH

    更新日期:2010-02-01 00:00:00

  • SERK and APOSTART. Candidate genes for apomixis in Poa pratensis.

    abstract::Seed production generally requires the mating of opposite sex gametes. Apomixis, an asexual mode of reproduction, avoids both meiotic reduction and egg fertilization. The essential feature of apomixis is that an embryo is formed autonomously by parthenogenesis from an unreduced egg of an embryo sac generated through a...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.105.062059

    authors: Albertini E,Marconi G,Reale L,Barcaccia G,Porceddu A,Ferranti F,Falcinelli M

    更新日期:2005-08-01 00:00:00

  • Novel Technique for Measuring Tissue Firmness within Tomato (Lycopersicon esculentum Mill.) Fruit.

    abstract::Developmental changes of tomato (Lycopersicon esculentum) fruit tissues during maturation were analyzed by a physically defined method (stress-relaxation analysis). The tip of a conical probe connected to a load sensor was positioned on the cut surface of a sliced tomato fruit, and the decay of the imposed stress was ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.96.2.545

    authors: Kojima K,Sakurai N,Kuraishi S,Yamamoto R,Nevins DJ

    更新日期:1991-06-01 00:00:00

  • Biosynthesis of Indol-3-yl-acetyl-myo-inositol Arabinoside in Kernels of Zea mays L.

    abstract::Extracts of immature kernels of Zea mays L. catalyzed the synthesis of indol-3-yl-acetyl-myo-inositol arabinoside from indol-3-yl-acetyl-myo-inositol and UDP-[U-(14)C]xylose. The product contained radioactivity which upon hydrolysis with trifluoroacetic acid cochromatographed with arabinose and not xylose. The amount ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.70.6.1664

    authors: Corcuera LJ,Bandurski RS

    更新日期:1982-12-01 00:00:00

  • Cellular distribution of polysome populations of castor bean endosperm.

    abstract::The distribution of polysomes in the cells of the endosperm of very young (48 hours of germination) seedlings of castor bean (Ricinus communis var. Hale) has been examined. Seedlings exposed to 100 micromolar gibberellin A(3) for periods up to 24 hours showed increased numbers of polysomes associated with endomembrane...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.65.2.359

    authors: González E

    更新日期:1980-02-01 00:00:00

  • Effect of Temperature, CO(2) Concentration, and Light Intensity on Oxygen Inhibition of Photosynthesis in Wheat Leaves.

    abstract::The effect of 21% O(2) and 3% O(2) on the CO(2) exchange of detached wheat leaves was measured in a closed system with an infrared carbon dioxide analyzer. Temperature was varied between 2 degrees and 43 degrees , CO(2) concentration between 0.000% and 0.050% and light intensity between 40 ft-c and 1000 ft-c. In most ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.43.6.902

    authors: Jolliffe PA,Tregunna EB

    更新日期:1968-06-01 00:00:00

  • Freeze-thaw stress: effects of temperature on hydraulic conductivity and ultrasonic activity in ten woody angiosperms.

    abstract::Freeze-thaw events can affect plant hydraulics by inducing embolism. This study analyzed the effect of temperature during the freezing process on hydraulic conductivity and ultrasonic emissions (UE). Stems of 10 angiosperms were dehydrated to a water potential at 12% percentage loss of hydraulic conductivity (PLC) and...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.113.228403

    authors: Charrier G,Charra-Vaskou K,Kasuga J,Cochard H,Mayr S,Améglio T

    更新日期:2014-02-01 00:00:00

  • Abscisic Acid promotes both volume flow and ion release to the xylem in sunflower roots.

    abstract::The effect of abscisic acid (ABA) on the exudation process in excised sunflower root was investigated. A promotion of both ion flux and volume exudation rate was observed. Cutting off the external supply of ions to the roots markedly increased the magnitude of the ABA effect. The promotive effect of ABA on exudation r...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.65.3.537

    authors: Glinka Z

    更新日期:1980-03-01 00:00:00

  • Tri-Hydroxy-Triacylglycerol Is Efficiently Produced by Position-Specific Castor Acyltransferases.

    abstract::Understanding the biochemistry of triacylglycerol (TAG) assembly is critical in tailoring seed oils to produce high-value products. Hydroxy-fatty acid (HFA) is one such valuable modified fatty acid, which can be produced at low levels in Arabidopsis (Arabidopsis thaliana) seed through transgenic expression of the cast...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.18.01409

    authors: Lunn D,Wallis JG,Browse J

    更新日期:2019-03-01 00:00:00

  • Regulation of ADP-Glucose Pyrophosphorylase from Chlorella vulgaris.

    abstract::ADP-glucose pyrophosphorylase was partially purified from Chlorella vulgaris 11h. 3-Phosphoglycerate activated the enzyme by lowering the Michaelis constant for glucose-1-phosphate (from 0.97 to 0.36 millimolar in the presence of 2 millimolar phosphoglycerate) and ATP (from 0.23 to 0.10 millimolar), as well as increas...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.78.3.601

    authors: Nakamura Y,Imamura M

    更新日期:1985-07-01 00:00:00

  • Hydroxycinnamoyl-CoA:Putrescine Hydroxycinnamoyltransferase in Tobacco Cell Cultures with High and Low Levels of Caffeoylputrescine.

    abstract::A new hydroxycinnamoyl-CoA:putrescine hydroxycinnamoyltransferase (PHT) was detected in two variant lines of Nicotiana tabacum L. (TX1, TX4) accumulating markedly different levels of caffeoylputrescine. The enzyme accepted only the aliphatic diamines putrescine, cadaverine and 1,3-diaminopropane at a ratio of 100:33:8...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.89.2.488

    authors: Meurer-Grimes B,Berlin J,Strack D

    更新日期:1989-02-01 00:00:00

  • Increased Ethylene Production during Clinostat Experiments May Cause Leaf Epinasty.

    abstract::Ethylene production from tomato (Lycopersicum esculentum L. cv. Rutgers) plants based on a clinostat doubled during the first 2 hours of rotation. Carbon dioxide blocked the appearance of leaf epinasty normally associated with plants rotated on a clinostat. These results support the idea that epinasty of clinostated p...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.49.2.183

    authors: Leather GR,Forrence LE

    更新日期:1972-02-01 00:00:00

  • Effects of vanadate on the plasma membrane ATPase of red beet and corn.

    abstract::The effect of vanadate on the plant plasma membrane ATPase were investigated in plasma membrane fractions derived from corn roots (Zea mays L.) and red beets (Beta vulgaris L.). The K(i) for vanadate inhibition of the plasma membrane ATPase from corn roots and red beets was between 6 and 15 micromolar vanadate. In bot...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.75.3.586

    authors: O'neill SD,Spanswick RM

    更新日期:1984-07-01 00:00:00

  • Nitrogen Metabolism in Soybean Tissue Culture: II. Urea Utilization and Urease Synthesis Require Ni.

    abstract::Potassium citrate (10 mM, pH 6) inhibits the growth of cultured (Glycine max L.) cells when urea is the sole nitrogen source. Ureadependent citrate toxicity is overcome by three separate additions to the growth medium: (a) NH(4)Cl (20 mM); (b) high levels of MgCl(2) (10 mM) or CaCl(2) (5-10 mM); (c) low levels of NiSO...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.59.5.827

    authors: Polacco JC

    更新日期:1977-05-01 00:00:00

  • Dehydroascorbate influences the plant cell cycle through a glutathione-independent reduction mechanism.

    abstract::Glutathione is generally accepted as the principal electron donor for dehydroascorbate (DHA) reduction. Moreover, both glutathione and DHA affect cell cycle progression in plant cells. But other mechanisms for DHA reduction have been proposed. To investigate the connection between DHA and glutathione, we have evaluate...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.103.033548

    authors: Potters G,Horemans N,Bellone S,Caubergs RJ,Trost P,Guisez Y,Asard H

    更新日期:2004-04-01 00:00:00

  • CHLOROPLAST RIBOSOME ASSOCIATED Supports Translation under Stress and Interacts with the Ribosomal 30S Subunit.

    abstract::Chloroplast ribosomes, which originated from cyanobacteria, comprise a large subunit (50S) and a small subunit (30S) containing ribosomal RNAs (rRNAs) and various ribosomal proteins. Genes for many chloroplast ribosomal proteins, as well as proteins with auxiliary roles in ribosome biogenesis or functioning, reside in...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.18.00602

    authors: Pulido P,Zagari N,Manavski N,Gawronski P,Matthes A,Scharff LB,Meurer J,Leister D

    更新日期:2018-08-01 00:00:00

  • A Ca/H Antiport System Driven by the Proton Electrochemical Gradient of a Tonoplast H-ATPase from Oat Roots.

    abstract::Two types of ATP-dependent calcium (Ca(2+)) transport systems were detected in sealed microsomal vesicles from oat roots. Approximately 80% of the total Ca(2+) uptake was associated with vesicles of 1.11 grams per cubic centimeter and was insensitive to vanadate or azide, but inhibited by NO(3) (-). The remaining 20% ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.79.4.1111

    authors: Schumaker KS,Sze H

    更新日期:1985-12-01 00:00:00

  • Transcription Factor Interplay between LEAFY and APETALA1/CAULIFLOWER during Floral Initiation.

    abstract::The transcription factors LEAFY (LFY) and APETALA1 (AP1), together with the AP1 paralog CAULIFLOWER (CAL), control the onset of flower development in a partially redundant manner. This redundancy is thought to be mediated, at least in part, through the regulation of a shared set of target genes. However, whether these...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.17.00098

    authors: Goslin K,Zheng B,Serrano-Mislata A,Rae L,Ryan PT,Kwaśniewska K,Thomson B,Ó'Maoiléidigh DS,Madueño F,Wellmer F,Graciet E

    更新日期:2017-06-01 00:00:00

  • Hormonal regulation of cell elongation in the hypocotyl of rootless soybean: an evaluation of the role of DNA synthesis.

    abstract::A method was developed where soybean seedlings were grown without roots to study the influence of hormones of root origin on shoot growth. Excision of the root resulted in inhibition of apical section growth and DNA synthesis and inhibited elongating section growth. A synthetic cytokinin restored DNA synthesis in the ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.44.9.1295

    authors: Holm RE,Key JL

    更新日期:1969-09-01 00:00:00

  • Isomerization of 1-O-indol-3-ylacetyl-beta-D-glucose. Enzymatic hydrolysis of 1-O, 4-O, and 6-O-indol-3-ylacetyl-beta-D-glucose and the enzymatic synthesis of indole-3-acetyl glycerol by a hormone metabolizing complex.

    abstract::The first compound in the series of reactions leading to the ester conjugates of indole-3-acetic acid (IAA) in kernels of Zea mays sweet corn is the acyl alkyl acetal, 1-O-indol-3-ylacetyl-beta-D-glucose (1-O-IAGlu). The enzyme catalyzing the synthesis of this compound is UDP-glucose:indol-3-ylacetate glucosyl-transf...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.94.1.4

    authors: Kowalczyk S,Bandurski RS

    更新日期:1990-01-01 00:00:00

  • The C-Domain of Oleuropein β-Glucosidase Assists in Protein Folding and Sequesters the Enzyme in Nucleus.

    abstract::Oleuropein, a terpene-derived glycosylated secoiridoid biosynthesized exclusively by members of the Oleaceae family, is involved in a two-component defense system comprising a β-glucosidase that activates oleuropein into a toxic glutaraldehyde-like structure. Oleuropein and its deglycosylated derivatives have high pha...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.17.00512

    authors: Koudounas K,Thomopoulou M,Michaelidis C,Zevgiti E,Papakostas G,Tserou P,Daras G,Hatzopoulos P

    更新日期:2017-07-01 00:00:00

  • Separation of the Mg-ATPases from the Ca-Phosphatase Activity of Microsomal Membranes Prepared from Barley Roots.

    abstract::Two methods for preparing membrane fractions from barley (Hordeum vulgare cv California Mariout 72) roots were compared in order to resolve reported differences between the characteristics of the plasma membrane ATPase of barley and that of other species. When microsomal membranes were prepared by a published procedur...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.77.4.857

    authors: Dupont FM,Hurkman WJ

    更新日期:1985-04-01 00:00:00

  • R2R3-NaMYB8 regulates the accumulation of phenylpropanoid-polyamine conjugates, which are essential for local and systemic defense against insect herbivores in Nicotiana attenuata.

    abstract::Although phenylpropanoid-polyamine conjugates (PPCs) occur ubiquitously in plants, their biological roles remain largely unexplored. The two major PPCs of Nicotiana attenuata plants, caffeoylputrescine (CP) and dicaffeoylspermidine, increase dramatically in local and systemic tissues after herbivore attack and simulat...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.109.151738

    authors: Kaur H,Heinzel N,Schöttner M,Baldwin IT,Gális I

    更新日期:2010-03-01 00:00:00

  • Five Nodulation Mutants of White Sweetclover (Melilotus alba Desr.) Exhibit Distinct Phenotypes Blocked at Root Hair Curling, Infection Thread Development, and Nodule Organogenesis.

    abstract::In an effort to obtain a developmental sequence of mutations in the Rhizobium-legume interaction within a single legume species, we have characterized the early events of nodule development in 10 nodulation mutants of sweetclover, Melilotus alba Desr. cv U389, representing five genetic loci. Both seed and root exudate...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.103.3.925

    authors: Utrup LJ,Cary AJ,Norris JH

    更新日期:1993-11-01 00:00:00

  • A β-Ketoacyl-CoA Synthase Is Involved in Rice Leaf Cuticular Wax Synthesis and Requires a CER2-LIKE Protein as a Cofactor.

    abstract::Cuticular waxes are complex mixtures of very-long-chain fatty acids (VLCFAs) and their derivatives, forming a natural barrier on aerial surfaces of terrestrial plants against biotic and abiotic stresses. In VLCFA biosynthesis, β-ketoacyl-CoA synthase (KCS) is the key enzyme, catalyzing the first reaction in fatty acid...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.16.01527

    authors: Wang X,Guan Y,Zhang D,Dong X,Tian L,Qu LQ

    更新日期:2017-02-01 00:00:00

  • Predicting Dynamic Metabolic Demands in the Photosynthetic Eukaryote Chlorella vulgaris.

    abstract::Phototrophic organisms exhibit a highly dynamic proteome, adapting their biomass composition in response to diurnal light/dark cycles and nutrient availability. Here, we used experimentally determined biomass compositions over the course of growth to determine and constrain the biomass objective function (BOF) in a ge...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.17.00605

    authors: Zuñiga C,Levering J,Antoniewicz MR,Guarnieri MT,Betenbaugh MJ,Zengler K

    更新日期:2018-01-01 00:00:00

  • Is there a third photoreceptor involved in the control of chloroplast movements in mougeotia?

    abstract::The photometric method was used to test a possibility proposed recently that a new photoreceptor with maximum activity at 620 nm is involved in mediating chloroplast rotation in Mougeotia (Z Lechowski, J Bialczyk [1988] Plant Physiol 88: 189-193). The hypothesis was tested under conditions of continuous dichromatic un...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.94.1.221

    authors: Walczak T,Gabrỳs H,Appenroth KJ

    更新日期:1990-09-01 00:00:00

  • Characterization of MdMADS2, a member of the SQUAMOSA subfamily of genes, in apple.

    abstract::A MADS-box gene, MdMADS2, was isolated from the apple (Malus x domestica Borkh.) var Fuji and its developmental expression pattern was studied during flower development. MdMADS2 shares a high degree of amino acid sequence identity with the SQUAMOSA subfamily of genes. RNA blot analysis showed that MdMADS2 is transcrib...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.120.4.969

    authors: Sung SK,Yu GH,An G

    更新日期:1999-08-01 00:00:00

  • Arabidopsis NATA1 Acetylates Putrescine and Decreases Defense-Related Hydrogen Peroxide Accumulation.

    abstract::Biosynthesis of the polyamines putrescine, spermidine, and spermine is induced in response to pathogen infection of plants. Putrescine, which is produced from Arg, serves as a metabolic precursor for longer polyamines, including spermidine and spermine. Polyamine acetylation, which has important regulatory functions i...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.16.00446

    authors: Lou YR,Bor M,Yan J,Preuss AS,Jander G

    更新日期:2016-06-01 00:00:00

  • arc6, A Fertile Arabidopsis Mutant with Only Two Mesophyll Cell Chloroplasts.

    abstract::A novel mutant of Arabidopsis thaliana, arc6 (accumulation and replication of chloroplasts), has been isolated from a transfer DNA-mutagenized population of Arabidopsis seedlings. arc6 has the most extreme arc mutant phenotype we have yet described, with only one to three chloroplasts per leaf mesophyll cell compared ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.106.3.1169

    authors: Pyke KA,Rutherford SM,Robertson EJ,Leech RM

    更新日期:1994-11-01 00:00:00