Cinnamyl Alcohol Dehydrogenase: Identification of New Sites of Promoter Activity in Transgenic Poplar.

Abstract:

:Stem sections from poplar that were stably transformed with a eucalypt cinnamyl alcohol dehydrogenase promoter-[beta]-glucuronidase construct were prepared by using either a technique routinely used in herbaceous species or a technique designed to take into account the particular anatomy of woody plants. Although both preparation techniques confirmed the pattern of expression previously observed (C. Feuillet, V. Lauvergeat, C. Deswarte, G. Pilate, A. Boudet and J. Grima-Pettenati [1995] Plant Mol Biol 27: 651-657), the latter technique also allowed the detection of other sites of promoter activity not revealed by the first technique. In situ hybridization confirmed the expression pattern obtained with the second sample preparation technique.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Hawkins S,Samaj J,Lauvergeat V,Boudet A,Grima-Pettenati J

doi

10.1104/pp.113.2.321

subject

Has Abstract

pub_date

1997-02-01 00:00:00

pages

321-325

issue

2

eissn

0032-0889

issn

1532-2548

pii

113/2/321

journal_volume

113

pub_type

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