A comprehensive analysis of microProteins reveals their potentially widespread mechanism of transcriptional regulation.

Abstract:

:Truncated transcription factor-like proteins called microProteins (miPs) can modulate transcription factor activities, thereby increasing transcriptional regulatory complexity. To understand their prevalence, evolution, and function, we predicted over 400 genes that encode putative miPs from Arabidopsis (Arabidopsis thaliana) using a bioinformatics pipeline and validated two novel miPs involved in flowering time and response to abiotic and biotic stress. We provide an evolutionary perspective for a class of miPs targeting homeodomain transcription factors in plants and metazoans. We identify domain loss as one mechanism of miP evolution and suggest the possible roles of miPs on the evolution of their target transcription factors. Overall, we reveal a prominent layer of transcriptional regulation by miPs, show pervasiveness of such proteins both within and across genomes, and provide a framework for studying their function and evolution.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Magnani E,de Klein N,Nam HI,Kim JG,Pham K,Fiume E,Mudgett MB,Rhee SY

doi

10.1104/pp.114.235903

subject

Has Abstract

pub_date

2014-05-01 00:00:00

pages

149-59

issue

1

eissn

0032-0889

issn

1532-2548

pii

pp.114.235903

journal_volume

165

pub_type

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