Role of petal-specific orcinol O-methyltransferases in the evolution of rose scent.

Abstract:

:Orcinol O-methyltransferase (OOMT) 1 and 2 catalyze the last two steps of the biosynthetic pathway leading to the phenolic methyl ether 3,5-dimethoxytoluene (DMT), the major scent compound of many rose (Rosa x hybrida) varieties. Modern roses are descended from both European and Chinese species, the latter being producers of phenolic methyl ethers but not the former. Here we investigated why phenolic methyl ether production occurs in some but not all rose varieties. In DMT-producing varieties, OOMTs were shown to be localized specifically in the petal, predominantly in the adaxial epidermal cells. In these cells, OOMTs become increasingly associated with membranes during petal development, suggesting that the scent biosynthesis pathway catalyzed by these enzymes may be directly linked to the cells' secretory machinery. OOMT gene sequences were detected in two non-DMT-producing rose species of European origin, but no mRNA transcripts were detected, and these varieties lacked both OOMT protein and enzyme activity. These data indicate that up-regulation of OOMT gene expression may have been a critical step in the evolution of scent production in roses.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Scalliet G,Lionnet C,Le Bechec M,Dutron L,Magnard JL,Baudino S,Bergougnoux V,Jullien F,Chambrier P,Vergne P,Dumas C,Cock JM,Hugueney P

doi

10.1104/pp.105.070961

subject

Has Abstract

pub_date

2006-01-01 00:00:00

pages

18-29

issue

1

eissn

0032-0889

issn

1532-2548

pii

pp.105.070961

journal_volume

140

pub_type

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