Pyruvate Dehydrogenase Complex from Chloroplasts of Pisum sativum L.

Abstract:

:Pyruvate dehydrogenase complex is associated with intact chloroplasts and mitochondria of 9-day-old Pisum sativum L. seedlings. The ratio of the mitochondrial complex to the chloroplast complex activities is about 3 to 1. Maximal rates observed for chloroplast pyruvate dehydrogenase complex activity ranged from 6 to 9 micromoles of NADH produced per milligram of chlorophyll per hour. Osmotic rupture of pea chloroplasts released 88% of the complex activity, indicating that chloroplast pyruvate dehydrogenase complex is a stromal complex. The pH optimum for chloroplast pyruvate dehydrogenase complex was between 7.8 and 8.2, whereas the mitochondrial pyruvate dehydrogenase complex had a pH optimum between 7.3 and 7.7. Chloroplast pyruvate dehydrogenase complex activity was specific for pyruvate, dependent upon coenzyme A and NAD and partially dependent upon Mg(2+) and thiamine pyrophosphate.Chloroplast-associated pyruvate dehydrogenase complex provides a direct link between pyruvate metabolism and chloroplast fatty acid biosynthesis by providing the substrate, acetyl-CoA, necessary for membrane development in young plants.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Williams M,Randall DD

doi

10.1104/pp.64.6.1099

subject

Has Abstract

pub_date

1979-12-01 00:00:00

pages

1099-103

issue

6

eissn

0032-0889

issn

1532-2548

journal_volume

64

pub_type

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