Expression of water channel proteins in Mesembryanthemum crystallinum.

Abstract:

:We have characterized transcripts for nine major intrinsic proteins (MIPs), some of which function as water channels (aquaporins), from the ice plant Mesembryanthemum crystallinum. To determine the cellular distribution and expression of these MIPs, oligopeptide-based antibodies were generated against MIP-A, MIP-B, MIP-C, or MIP-F, which, according to sequence and functional characteristics, are located in the plasma membrane (PM) and tonoplast, respectively. MIPs were most abundant in cells involved in bulk water flow and solute flux. The tonoplast MIP-F was found in all cells, while signature cell types identified different PM-MIPs: MIP-A predominantly in phloem-associated cells, MIP-B in xylem parenchyma, and MIP-C in the epidermis and endodermis of immature roots. Membrane protein analysis confirmed MIP-F as tonoplast located. MIP-A and MIP-B were found in tonoplast fractions and also in fractions distinct from either the tonoplast or PM. MIP-C was most abundant but not exclusive to PM fractions, where it is expected based on its sequence signature. We suggest that within the cell, MIPs are mobile, which is similar to aquaporins cycling through animal endosomes. MIP cycling and the differential regulation of these proteins observed under conditions of salt stress may be fundamental for the control of tissue water flux.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Kirch HH,Vera-Estrella R,Golldack D,Quigley F,Michalowski CB,Barkla BJ,Bohnert HJ

doi

10.1104/pp.123.1.111

subject

Has Abstract

pub_date

2000-05-01 00:00:00

pages

111-24

issue

1

eissn

0032-0889

issn

1532-2548

journal_volume

123

pub_type

杂志文章
  • SHORT-ROOT and SCARECROW regulate leaf growth in Arabidopsis by stimulating S-phase progression of the cell cycle.

    abstract::SHORT-ROOT (SHR) and SCARECROW (SCR) are required for stem cell maintenance in the Arabidopsis (Arabidopsis thaliana) root meristem, ensuring its indeterminate growth. Mutation of SHR and SCR genes results in disorganization of the quiescent center and loss of stem cell activity, resulting in the cessation of root gro...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.110.158857

    authors: Dhondt S,Coppens F,De Winter F,Swarup K,Merks RM,Inzé D,Bennett MJ,Beemster GT

    更新日期:2010-11-01 00:00:00

  • Trithorax group protein Oryza sativa Trithorax1 controls flowering time in rice via interaction with early heading date3.

    abstract::Trithorax group proteins are chromatin-remodeling factors that activate target gene expression by antagonistically functioning against the Polycomb group. In Arabidopsis (Arabidopsis thaliana), Arabidopsis Trithorax protein1 (ATX1) regulates flowering time and floral organ identity. Here, we observed that suppression ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.113.228049

    authors: Choi SC,Lee S,Kim SR,Lee YS,Liu C,Cao X,An G

    更新日期:2014-03-01 00:00:00

  • Intercellular localization of nitrate reductase in roots.

    abstract::Experiments were conducted with segments of corn roots to investigate whether nitrate reductase (NR) is compartmentalized in particular groups of cells that collectively form the root symplastic pathway. A microsurgical technique was used to separate cells of the epidermis, of the cortex, and of the stele. The presenc...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.82.3.675

    authors: Rufty TW,Thomas JF,Remmler JL,Campbell WH,Volk RJ

    更新日期:1986-11-01 00:00:00

  • Characterization of cadmium uptake by plant tissue.

    abstract::The uptake of cadmium by excised root tissue of barley (Hordeum vulgare L. cv. Arivat) was investigated with respect to kinetics, concentration, and interactions with various cations. The role of metabolism in Cd absorption was examined using a range of temperatures, anaerobic treatments, and chemical inhibitors. The ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.54.1.67

    authors: Cutler JM,Rains DW

    更新日期:1974-07-01 00:00:00

  • Inhibition of adenosine triphosphatase activity of the plasma membrane fraction of oat roots by diethylstilbestrol.

    abstract::Diethylstibestrol (DES) inhibited noncompetitively the ATPase in the plasma membrane fraction from Avena sativa L. cv. Goodfield roots when assayed in the presence of MgSO(4) or MgSO(4) plus KCl. In the presence of MgSO(4), 7.1x10(-5) molar DES inhibited the enzyme 50%; whereas in the presence of MgSO(4) and KCl, 1.3x...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.63.1.48

    authors: Balke NE,Hodges TK

    更新日期:1979-01-01 00:00:00

  • Lipid synthesis in the presence of nitrogenous compounds in Chlorella pyrenoidosa.

    abstract::Acetate was incorporated by Chlorella pyrenoidosa into nonvolatile water-soluble compounds (amino acids, organic acids and carbohydrates) with pH optimum between 4 and 5. Incorporation into lipid was maximal at pH 7.5. The proportion of incorporated acetate in lipid was not significantly affected by acetate concentrat...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.41.3.506

    authors: Yung KH,Mudd JB

    更新日期:1966-03-01 00:00:00

  • Anion-Sensitive, H-Pumping ATPase of Oat Roots : Direct Effects of Cl, NO(3), and a Disulfonic Stilbene.

    abstract::To understand the mechanism and molecular properties of the tonoplast-type H(+)-translocating ATPase, we have studied the effect of Cl(-), NO(3) (-), and 4,4'-diisothiocyano-2,2'-stilbene disulfonic acid (DIDS) on the activity of the electrogenic H(+)-ATPase associated with low-density microsomal vesicles from oat roo...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.76.2.490

    authors: Churchill KA,Sze H

    更新日期:1984-10-01 00:00:00

  • The lys5 mutations of barley reveal the nature and importance of plastidial ADP-Glc transporters for starch synthesis in cereal endosperm.

    abstract::Much of the ADP-Glc required for starch synthesis in the plastids of cereal endosperm is synthesized in the cytosol and transported across the plastid envelope. To provide information on the nature and role of the plastidial ADP-Glc transporter in barley (Hordeum vulgare), we screened a collection of low-starch mutant...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.104.045203

    authors: Patron NJ,Greber B,Fahy BF,Laurie DA,Parker ML,Denyer K

    更新日期:2004-08-01 00:00:00

  • The occurrence of phytoferritin and its relationship to effectiveness of soybean nodules.

    abstract::Polyacrylamide gel electrophoresis and electron microscopy revealed that accumulation of iron-protein in soybean nodules is influenced by nodule age, mutation in bradyrhizobia, and rhizobial/bradyrhizobial strain-soybean cultivar interactions. Iron-protein concentrations (micrograms per milligram protein) were inverse...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.83.2.299

    authors: Ko MP,Huang PY,Huang JS,Barker KR

    更新日期:1987-02-01 00:00:00

  • Concomitant determination of folar nitrogen loss, net carbon dioxide uptake, and transpiration.

    abstract::A closed system was designed for concomitant determination of net CO(2) uptake, transpiration, and foliar nitrogen (N) loss in soybeans (Glycine max [L.] Merr.). The CO(2) uptake was monitored by infrared analysis of system air removed periodically in aliquots. Leaf vapors were trapped in calibrated tubes at Dry Ice t...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.65.2.403

    authors: Weiland RT,Stutte CA

    更新日期:1980-02-01 00:00:00

  • L1 division and differentiation patterns influence shoot apical meristem maintenance.

    abstract::Plant development requires regulation of both cell division and differentiation. The class 1 KNOTTED1-like homeobox (KNOX) genes such as knotted1 (kn1) in maize (Zea mays) and SHOOTMERISTEMLESS in Arabidopsis (Arabidopsis thaliana) play a role in maintaining shoot apical meristem indeterminacy, and their misexpression...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.105.076075

    authors: Kessler S,Townsley B,Sinha N

    更新日期:2006-08-01 00:00:00

  • Reduced expression of succinyl-coenzyme A ligase can be compensated for by up-regulation of the gamma-aminobutyrate shunt in illuminated tomato leaves.

    abstract::Increasing experimental evidence suggests that the tricarboxylic acid cycle in plants is of greater importance in illuminated photosynthetic tissues than previously thought. In this study, transgenic tomato (Solanum lycopersicum) plants expressing a fragment of the beta-subunit of succinyl-coenzyme A ligase in either ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.107.103101

    authors: Studart-Guimarães C,Fait A,Nunes-Nesi A,Carrari F,Usadel B,Fernie AR

    更新日期:2007-11-01 00:00:00

  • Emission of Hydrogen Sulfide by Leaf Tissue in Response to l-Cysteine.

    abstract::Leaf discs and detached leaves exposed to l-cysteine emitted a volatile sulfur compound which was proven by gas chromatography to be H(2)S. This phenomenon was demonstrated in all nine species tested (Cucumis sativus, Cucurbita pepo, Nicotiana tabacum, Coleus blumei, Beta vulgaris, Phaseolus vulgaris, Medicago sativa,...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.70.2.430

    authors: Sekiya J,Schmidt A,Wilson LG,Filner P

    更新日期:1982-08-01 00:00:00

  • Malate Metabolism in Leaf Mitochondria from the Crassulacean Acid Metabolism Plant Kalanchoë blossfeldiana Poelln.

    abstract::The mechanisms and the controlling factors of malate oxidation by mitochondria from leaves of Kalanchoë blossfeldiana Poelln. plants performing Crassulacean acid metabolism were investigated using Percollpurified mitochondria. The effects of pH and of various cofactors (ATP, NAD(+), coenzyme A) on malate dehydrogenase...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.81.4.1039

    authors: Rustin P,Lance C

    更新日期:1986-08-01 00:00:00

  • Superoxide Dismutases: II. Purification and Quantitative Relationship with Water-soluble Protein in Seedlings.

    abstract::Superoxide dismutase was purified from pea (Pisum sativum L., cv. Wando) seeds and corn (Zea mays L., cv. Michigan 500) seedlings. The purified pea enzyme eluting as a single peak from gel exclusion chromatography columns contained the three electrophoretically distinct bands of superoxide dismutase characterizing the...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.59.2.315

    authors: Giannopolitis CN,Ries SK

    更新日期:1977-02-01 00:00:00

  • Reprogramming the phenylpropanoid metabolism in seeds of oilseed rape by suppressing the orthologs of reduced epidermal fluorescence1.

    abstract::As a result of the phenylpropanoid pathway, many Brassicaceae produce considerable amounts of soluble hydroxycinnamate conjugates, mainly sinapate esters. From oilseed rape (Brassica napus), we cloned two orthologs of the Arabidopsis (Arabidopsis thaliana) gene reduced epidermal fluorescence1 (REF1) encoding a conifer...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.113.215491

    authors: Mittasch J,Böttcher C,Frolov A,Strack D,Milkowski C

    更新日期:2013-04-01 00:00:00

  • Cell-specific photosynthetic gene expression in maize determined using cell separation techniques and hybridization in situ.

    abstract::Bundle sheath strands and mesophyll cell extracts have been isolated from maize (Zea mays L.) leaves using a mechanical disruption-filtration technique. Northern blot analysis showed that phosphoenolpyruvate carboxylase (PEPCase; EC 4.1.1.31) mRNA accumulates only in mesophyll cells. The mechanisms regulating the cell...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.82.2.613

    authors: Martineau B,Taylor WC

    更新日期:1986-10-01 00:00:00

  • Nickel: a micronutrient essential for higher plants.

    abstract::Nickel was established as an essential micronutrient for the growth of temperate cereal crops. Grain from barley (Hordeum vulgare L. cv ;Onda'; containing 40 to 80 nanograms of Ni per gram dry weight) grown in solution culture with negligible Ni concentrations (< 30 nanograms of Ni per liter) exhibited greatly reduced...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.85.3.801

    authors: Brown PH,Welch RM,Cary EE

    更新日期:1987-11-01 00:00:00

  • Phosphatidylinositol specific phospholipase C of plant stems : membrane associated activity concentrated in plasma membranes.

    abstract::A phosphatidylinositol-specific phospholipase C of plant stems (EC 3.1.4.10) assayed at pH 6.6 and at 30 degrees C cleaved phosphatidylinositol such that more than 85% of the product was inositol-1-phosphate. Other phospholipids were cleaved 5 to 10% or less under these conditions. The phospholipase had both a soluble...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.85.4.1151

    authors: Pfaffmann H,Hartmann E,Brightman AO,Morré DJ

    更新日期:1987-12-01 00:00:00

  • Identification of negative cis-acting elements in response to copper in the chloroplastic iron superoxide dismutase gene of the moss Barbula unguiculata.

    abstract::Superoxide dismutases (SODs) are ubiquitous metalloenzymes that catalyze the dismutation of superoxide radicals. Chloroplasts have two isozymes, copper/zinc SOD (Cu/ZnSOD) and iron SOD (FeSOD), encoded by nuclear genes. Because bryophytes are considered as the earliest land plants, they are one of the most interesting...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.107.114868

    authors: Nagae M,Nakata M,Takahashi Y

    更新日期:2008-04-01 00:00:00

  • Directed mutation of the Rubisco large subunit of tobacco influences photorespiration and growth.

    abstract::The gene for the large subunit of Rubisco was specifically mutated by transforming the chloroplast genome of tobacco (Nicotiana tabacum). Codon 335 was altered to encode valine instead of leucine. The resulting mutant plants could not grow without atmospheric CO2 enrichment. In 0.3% (v/v) CO2, the mutant and wild-type...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.121.2.579

    authors: Whitney SM,von Caemmerer S,Hudson GS,Andrews TJ

    更新日期:1999-10-01 00:00:00

  • Purification and Characterization of Two Forms of Glutamine Synthetase from the Pedicel Region of Maize (Zea mays L.) Kernels.

    abstract::Maize (Zea mays L.) kernel pedicels, including vascular tissues, pedicel parenchyma, placento-chalazal tissue, and the surrounding pericarp, contained two forms of glutamine synthetase (EC 6.3.1.2), separable by anion exchange chromatography under mildly acidic conditions. The earlier-eluting activity (GS(p1)), but no...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.91.3.868

    authors: Muhitch MJ

    更新日期:1989-11-01 00:00:00

  • Modulation of oat mitochondrial ATPase activity by CA2+ and phytochrome.

    abstract::The activity of a Mg(2+)-dependent ATPase present in highly purified preparations of Avena mitochondria was photoreversibly modulated by red/far-red light treatments. These results were obtained either with mitochondria isolated from plants irradiated with white light prior to the extraction or with mitochondria isol...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.74.4.827

    authors: Serlin BS,Sopory SK,Roux SJ

    更新日期:1984-04-01 00:00:00

  • Signals involved in Arabidopsis resistance to Trichoplusia ni caterpillars induced by virulent and avirulent strains of the phytopathogen Pseudomonas syringae.

    abstract::Plants have evolved different but interconnected strategies to defend themselves against herbivorous insects and microbial pathogens. We used an Arabidopsis/Pseudomonas syringae pathosystem to investigate the impact of pathogen-induced defense responses on cabbage looper (Trichoplusia ni) larval feeding. Arabidopsis m...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.010815

    authors: Cui J,Jander G,Racki LR,Kim PD,Pierce NE,Ausubel FM

    更新日期:2002-06-01 00:00:00

  • Reaction Properties of the Ascorbic Acid Oxidase from Myrothecium verrucaria.

    abstract::Ascorbic acid oxidase activity in Myrothecium verrucaria extracts resulted in O(2) uptake exceeding 0.5 mole per mole of ascorbic acid and in CO(2) evolution. Measurement of oxidized ascorbic acid at completion of the reaction demonstrated that an average of 10% of the oxidized product disappeared. A comparison of the...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.41.10.1553

    authors: Lillehoj EB,Smith FG

    更新日期:1966-12-01 00:00:00

  • The synthetic elicitor 3,5-dichloroanthranilic acid induces NPR1-dependent and NPR1-independent mechanisms of disease resistance in Arabidopsis.

    abstract::Immune responses of Arabidopsis (Arabidopsis thaliana) are at least partially mediated by coordinated transcriptional up-regulation of plant defense genes, such as the Late/sustained Up-regulation in Response to Hyaloperonospora parasitica (LURP) cluster. We found a defined region in the promoter of the LURP member Ca...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.108.133678

    authors: Knoth C,Salus MS,Girke T,Eulgem T

    更新日期:2009-05-01 00:00:00

  • Isolation and Characterization of a Protein Associated with Carotene Globules in the Alga Dunaliella bardawil.

    abstract::The halotolerant alga Dunaliella bardawil accumulates very large amounts of [beta]-carotene when exposed to high light intensity. The accumulated [beta]-carotene is concentrated in small, oily globules within the chloroplast and has been suggested to protect the alga against photodamage by high irradiation (A. Ben-Amo...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.108.4.1657

    authors: Katz A,Jimenez C,Pick U

    更新日期:1995-08-01 00:00:00

  • Distinct Cellular Locations of Carbonic Anhydrases Mediate Carbon Dioxide Control of Stomatal Movements.

    abstract::Elevated carbon dioxide (CO2) in leaves closes stomatal apertures. Research has shown key functions of the β-carbonic anhydrases (βCA1 and βCA4) in rapid CO2-induced stomatal movements by catalytic transmission of the CO2 signal in guard cells. However, the underlying mechanisms remain unclear, because initial studies...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.15.00646

    authors: Hu H,Rappel WJ,Occhipinti R,Ries A,Böhmer M,You L,Xiao C,Engineer CB,Boron WF,Schroeder JI

    更新日期:2015-10-01 00:00:00

  • Vacuolar Transporters - Companions on a Longtime Journey.

    abstract::Biochemical and electrophysiological studies on plant vacuolar transporters became feasible in the late 1970s and early 1980s, when methods to isolate large quantities of intact vacuoles and purified vacuolar membrane vesicles were established. However, with the exception of the H+-ATPase and H+-PPase, which could be ...

    journal_title:Plant physiology

    pub_type: 杂志文章,评审

    doi:10.1104/pp.17.01481

    authors: Martinoia E

    更新日期:2018-02-01 00:00:00

  • Abundance of the Major Chloroplast Polypeptides during Development and Ripening of Tomato Fruits: An Immunological Study.

    abstract::During maturation and ripening of tomato (Lycopersicon esculentum, cv Tamar) fruits, there are differential changes in the steady state levels of chloroplast proteins. Western blot analysis indicated that with the exception of the core polypeptide of photosystem I (PSI) (subunit I) the whole complex disappears during ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.87.1.239

    authors: Livne A,Gepstein S

    更新日期:1988-05-01 00:00:00