Superoxide Dismutases: II. Purification and Quantitative Relationship with Water-soluble Protein in Seedlings.

abstract：:To study in detail the hypersensitive reaction, one of the major defense responses of plants against microbial infection, we used a model system of reduced complexity with cultured parsley (Petroselinum crispum) cells infected with the phytopathogenic fungus Phytophthora infestans. Experimental conditions were establi...

journal_title：Plant physiology

authors： Naton B,Hahlbrock K,Schmelzer E

更新日期：1996-09-01 00:00:00

abstract：:The problem of determining gas exchange rates from flow system data under nonsteady state conditions is analyzed. A correction factor is presented for obtaining constant rates under nonsteady state conditions. A general formula for obtaining any rate under nonsteady state conditions is also given. Turnover time is def...

journal_title：Plant physiology

authors： Marynick DS

更新日期：1975-11-01 00:00:00

abstract：:Through a biochemical and a genetic approach, we have identified several plant genes encoding methylthioribose (MTR) kinase, an enzyme involved in recycling of methionine through the methylthioadenosine (MTA) cycle. OsMTK1, an MTR kinase from rice (Oryza sativa), is 48.6 kD in size and shows cooperative kinetics with ...

journal_title：Plant physiology

authors： Sauter M,Cornell KA,Beszteri S,Rzewuski G

更新日期：2004-12-01 00:00:00

abstract：:A controlled flow of porphyrin metabolites is critical for organisms, but little is known about the control of porphyrin biosynthesis under environmental stress. We monitored transgenic rice (Oryza sativa) plants expressing Myxococcus xanthus protoporphyrinogen oxidase (PPO) for their response to drought stress. Trans...

journal_title：Plant physiology

authors： Phung TH,Jung HI,Park JH,Kim JG,Back K,Jung S

更新日期：2011-12-01 00:00:00

abstract：:Two maize hybrids were grown under growth chamber conditions on solution or vermiculite medium that contained 2.5, 7.5, or 15 millimolar nitrate. The objectives were to determine: (a) the effect of nitrate supply on N metabolism and growth and (b) the interrelationship between nitrate uptake, flux, and reduction on th...

journal_title：Plant physiology

authors： Reed AJ,Hageman RH

更新日期：1980-12-01 00:00:00

abstract：:An NADP-dependent dehydrogenase catalyzing the conversion of l-sorbosone to l-ascorbic acid has been isolated from Phaseolus vulgaris L. and Spinacia oleracea L. and partially purified. It is stable at -20 degrees C for up to 8 months. Molecular masses, as determined by gel filtration, were 21 and 29 kilodaltons for b...

journal_title：Plant physiology

authors： Loewus MW,Bedgar DL,Saito K,Loewus FA

更新日期：1990-11-01 00:00:00

abstract：:Although cytosolic free Ca(2+) mobilization induced by microbe/pathogen-associated molecular patterns is postulated to play a pivotal role in innate immunity in plants, the molecular links between Ca(2+) and downstream defense responses still remain largely unknown. Calcineurin B-like proteins (CBLs) act as Ca(2+) sen...

journal_title：Plant physiology

authors： Kurusu T,Hamada J,Nokajima H,Kitagawa Y,Kiyoduka M,Takahashi A,Hanamata S,Ohno R,Hayashi T,Okada K,Koga J,Hirochika H,Yamane H,Kuchitsu K

更新日期：2010-06-01 00:00:00

abstract：:The gene specifying plastid transketolase (TK) of maize (Zea mays) was cloned from a cDNA library by southern blotting using a heterologous probe from sorghum (Sorghum bicolor). A recombinant fusion protein comprising thioredoxin of Escherichia coli and mature TK of maize was expressed at a high level in E. coli and c...

journal_title：Plant physiology

authors： Gerhardt S,Echt S,Busch M,Freigang J,Auerbach G,Bader G,Martin WF,Bacher A,Huber R,Fischer M

更新日期：2003-08-01 00:00:00

abstract：:Orcinol O-methyltransferase (OOMT) 1 and 2 catalyze the last two steps of the biosynthetic pathway leading to the phenolic methyl ether 3,5-dimethoxytoluene (DMT), the major scent compound of many rose (Rosa x hybrida) varieties. Modern roses are descended from both European and Chinese species, the latter being produ...

journal_title：Plant physiology

authors： Scalliet G,Lionnet C,Le Bechec M,Dutron L,Magnard JL,Baudino S,Bergougnoux V,Jullien F,Chambrier P,Vergne P,Dumas C,Cock JM,Hugueney P

更新日期：2006-01-01 00:00:00

abstract：:Suspension cultures of carrot (Daucus carota, line C1), tobacco (Nicotiana tabacum, line TX1), and Nicotiana plumbaginifolia (line NP) were frozen under controlled conditions with trehalose as the sole cryoprotectant. Maximal post-thaw viability (71-74%), measured by phenosafranin dye exclusion, was obtained with the ...

journal_title：Plant physiology

authors： Bhandal IS,Hauptmann RM,Widholm JM

更新日期：1985-06-01 00:00:00

abstract：:Phosphoenolpyruvate partially inhibits the accumulation of Ca(2+) in isolated mung bean (Phaseolus aureus Roxb.) mitochondria. Succinate-supported Ca(2+) uptake is twice as sensitive to phosphoenolpyruvate inhibition as is NADH- or malate/pyruvate-supported Ca(2+) uptake. Pyruvate, atractylate, and ATP, but not ITP, r...

journal_title：Plant physiology

authors： Graesser RJ

更新日期：1977-02-01 00:00:00

abstract：:The lipid and protein composition of the plasma membrane isolated from mulberry (Morus bombycis Koidz.) bark cells was analyzed throughout the cold acclimation period under natural and controlled environment conditions. There was a significant increase in phospholipids and unsaturation of their fatty acids during cold...

journal_title：Plant physiology

authors： Yoshida S

更新日期：1984-09-01 00:00:00

abstract：:An NAD-linked dehydrogenase from Chlorella pyrenoidosa Chick catalyzing the conversion of l-proline to Delta(1)-pyrroline-5-carboxylic acid was partially purified. Delta(1)-Pyrroline-5-carboxylic acid was identified as the product by co-chromatography of it and its o-aminobenzaldehyde derivative with authentic compoun...

journal_title：Plant physiology

authors： McNamer AD,Stewart CR

更新日期：1974-03-01 00:00:00

abstract：:An acetylcholine hydrolyzing enzyme was prepared and purified (40 times) from dwarf bean hypocotyl hooks. The purity of the enzyme was proved by polyacrylamide gel electrophoresis. The molecular weight of the enzyme was determined to be 65,000 daltons. Enzyme activity was the highest at pH 8.0 and between 30 and 36 C....

journal_title：Plant physiology

authors： Ernst M,Hartmann E

更新日期：1980-03-01 00:00:00

abstract：:The levels of activity of 2-phosphoglycolate phosphatase in the green algae, Chlamydomonas reinhardtii and Chlorella vulgaris, were in the range of 37 to 60 micromoles per milligram chlorophyll per hour and in the blue-green algae, Anacystis nidulans and Anabaena variabilis were 204 to 310 micromoles per milligram chl...

journal_title：Plant physiology

authors： Husic HD,Tolbert NE

更新日期：1985-10-01 00:00:00

abstract：:With the aid of an extracellular vibrating electrode, natural electric fields were detected and measured in the medium near growing roots and root hairs of barley seedlings. An exploration of these fields indicates that both the root as a whole, as well as individual root hairs, drive large steady currents through the...

journal_title：Plant physiology

authors： Weisenseel MH,Dorn A,Jaffe LF

更新日期：1979-09-01 00:00:00

abstract：:Understanding the relationship of the size and shape of an organism to the size, shape, and number of its constituent cells is a basic problem in biology; however, numerous studies indicate that the relationship is complex and often nonintuitive. To investigate this problem, we used a system for the inducible expressi...

journal_title：Plant physiology

authors： Kuwabara A,Backhaus A,Malinowski R,Bauch M,Hunt L,Nagata T,Monk N,Sanguinetti G,Fleming A

更新日期：2011-08-01 00:00:00

abstract：:The sensitivity of lettuce (Lactuca sativa L. cv Grand Rapids) seeds to red light was reduced by NaCl concentrations which had no effect upon the germination of continuously illuminated seeds. The germination capacity of the seeds was fully restored by increased red light exposures. Indirect evidence indicates that Na...

journal_title：Plant physiology

authors： Scorer KN,Epel BL,Waisel Y

更新日期：1985-09-01 00:00:00

abstract：:Though Helminthosporium maydis race T (HmT) toxin decreased active Ca(2+) uptake into mitochondria isolated from susceptible (T) but not resistant (N) corn (Kimber, Sze, 1984 Plant Physiol 74: 804-809 the mode of toxin action is not understood. This study shows that HmT toxin or A23187 (a Ca(2+) ionophore) dissipated ...

journal_title：Plant physiology

authors： Holden MJ,Sze H

更新日期：1984-05-01 00:00:00

abstract：:Victorin, the pathotoxin from the host-specific pathogen, Helminthosporium victoriae, promotes the growth of coleoptile segments when given at concentrations that are high but which still show selective effects on susceptible and resistant tissue. The latent period in the growth response of both susceptible and resist...

journal_title：Plant physiology

authors： Saftner RA,Evans ML

更新日期：1974-03-01 00:00:00

abstract：:In this article, we demonstrate the connection between intracellular iron storage and oxidative stress response in cyanobacteria. Iron is essential for the survival of all organisms. However, the redox properties that make iron a valuable cofactor also lead to oxidative interactions, resulting in the formation of harm...

journal_title：Plant physiology

authors： Shcolnick S,Summerfield TC,Reytman L,Sherman LA,Keren N

更新日期：2009-08-01 00:00:00

abstract：:A full-genome microarray of the (oxy)photosynthetic cyanobacterium Synechocystis sp. PCC 6803 was used to identify genes that were transcriptionally regulated by growth in iron (Fe)-deficient versus Fe-sufficient media. Transcript accumulation for 3,165 genes in the genome was analyzed using an analysis of variance mo...

journal_title：Plant physiology

authors： Singh AK,McIntyre LM,Sherman LA

更新日期：2003-08-01 00:00:00

abstract：:The patterns of expression for genes encoding several C4 photosynthetic enzymes were examined in light-grown and dark-grown (etiolated) cotyledons of amaranth (Amaranthus hypochondriacus), a dicotyledonous C4 plant. The large subunit and small subunit of ribulose-1,5-bisphosphate carboxylase (RuBPCase), phosphoenolpyr...

journal_title：Plant physiology

authors： Wang JL,Long JJ,Hotchkiss T,Berry JO

更新日期：1993-08-01 00:00:00

abstract：:The rate of Cl(-) transport at the plasma membrane of the freshwater alga Chara corallina is investigated with respect to possible in vivo controls acting in addition to the two well established ones of cytoplasmic Cl(-) and cytoplasmic pH. In contrast with results from many other plant tissues, halides appear to be t...

journal_title：Plant physiology

authors： Sanders D

更新日期：1981-06-01 00:00:00

abstract：:Cotton (Gossypium spp.) fiber cells are seed trichomes derived from the epidermal layer of the cotton seed coat. The molecular components responsible for regulating fiber cell differentiation have not been fully elucidated. A cotton PROTODERMAL FACTOR1 gene (GbPDF1) was found to be expressed preferentially during fibe...

journal_title：Plant physiology

authors： Deng F,Tu L,Tan J,Li Y,Nie Y,Zhang X

更新日期：2012-02-01 00:00:00

abstract：:Terpenes are specialized plant metabolites that act as attractants to pollinators and as defensive compounds against pathogens and herbivores, but they also play an important role in determining the quality of horticultural food products. We show that the genome of cultivated apple (Malus domestica) contains 55 putati...

journal_title：Plant physiology

authors： Nieuwenhuizen NJ,Green SA,Chen X,Bailleul EJ,Matich AJ,Wang MY,Atkinson RG

更新日期：2013-02-01 00:00:00

abstract：:In pea leaves, much of the metabolism of imported asparagine is by transamination. This activity was previously shown to be localized in the peroxisomes, suggesting a possible connection between asparagine and photorespiratory nitrogen metabolism. This was investigated by examination of the transfer of (15)N from the ...

journal_title：Plant physiology

authors： Ta TC,Joy KW,Ireland RJ

更新日期：1985-06-01 00:00:00

abstract：:Previous work on the growth biophysics of maize (Zea mays L.) primary roots suggested that cell walls in the apical 5 mm of the elongation zone increased their yielding ability as an adaptive response to low turgor and water potential (psi w). To test this hypothesis more directly, we measured the acid-induced extens...

journal_title：Plant physiology

authors： Wu Y,Sharp RE,Durachko DM,Cosgrove DJ

更新日期：1996-07-01 00:00:00

abstract：:Mg-chelatase catalyzes the first step unique to the chlorophyll branch of tetrapyrrole biosynthesis, namely the insertion of Mg into protoporphyrin IX (Proto). Mg-chelatase was assayed in intact chloroplasts from semi-green cucumber (Cucumis sativus, cv Sumter) cotyledons. In the presence of Proto and MgATP, enzyme ac...

journal_title：Plant physiology

authors： Walker CJ,Weinstein JD

更新日期：1991-04-01 00:00:00

abstract：:We have used (13)C-labeled sugars and nuclear magnetic resonance (NMR) spectrometry to study the metabolic pathway of starch biosynthesis in developing wheat grain (Triticum aestivum cv Mardler). Our aim was to examine the extent of redistribution of (13)C between carbons atoms 1 and 6 of [1-(13)C] or [6-(13)C]glucose...

journal_title：Plant physiology

authors： Keeling PL,Wood JR,Tyson RH,Bridges IG

更新日期：1988-06-01 00:00:00