Abstract:
:Much of the ADP-Glc required for starch synthesis in the plastids of cereal endosperm is synthesized in the cytosol and transported across the plastid envelope. To provide information on the nature and role of the plastidial ADP-Glc transporter in barley (Hordeum vulgare), we screened a collection of low-starch mutants for lines with abnormally high levels of ADP-Glc in the developing endosperm. Three independent mutants were discovered, all of which carried mutations at the lys5 locus. Plastids isolated from the lys5 mutants were able to synthesize starch at normal rates from Glc-1-P but not from ADP-Glc, suggesting a specific lesion in the transport of ADP-Glc across the plastid envelope. The major plastidial envelope protein was purified, and its sequence showed it to be homologous to the maize (Zea mays) ADP-Glc transporter BRITTLE1. The gene encoding this protein in barley, Hv.Nst1, was cloned, sequenced, and mapped. Like lys5, Hv.Nst1 lies on chromosome 6(6H), and all three of the lys5 alleles that were examined were shown to carry lesions in Hv.Nst1. Two of the identified mutations in Hv.Nst1 lead to amino acid substitutions in a domain that is conserved in all members of the family of carrier proteins to which Hv.NST1 belongs. This strongly suggests that Hv.Nst1 lies at the Lys5 locus and encodes a plastidial ADP-Glc transporter. The low-starch phenotype of the lys5 mutants shows that the ADP-Glc transporter is required for normal rates of starch synthesis. This work on Hv.NST1, together with the earlier work on BRITTLE1, suggests that homologous transporters are probably present in the endosperm of all cereals.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Patron NJ,Greber B,Fahy BF,Laurie DA,Parker ML,Denyer Kdoi
10.1104/pp.104.045203subject
Has Abstractpub_date
2004-08-01 00:00:00pages
2088-97issue
4eissn
0032-0889issn
1532-2548pii
pp.104.045203journal_volume
135pub_type
杂志文章相关文献
PLANT PHYSIOLOGY文献大全abstract::We have described the modulation of four auxin-regulated genes during the growth cycle of suspension-cultured tobacco (Nicotiana tabacum [L.] var White Burley) cells. The genes were transiently expressed 2 to 8 h after transfer of stationary phase cells to fresh medium, during the transition from the quiescent phase o...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.102.2.513
更新日期:1993-06-01 00:00:00
abstract::Six species having characteristics of plants with the C(4) dicarboxylic photosynthetic pathway, Echinochloa utilis L. Ohwi et Yabuno (Japanese millet), Cynodon dactylon L. (Bermuda grass), Kyllinga brevifolia Rottb., Amaranthus tricolor L. cv. Early splendour, Kochia childsii Hort., and Portulaca grandiflora Hook (ros...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.49.5.794
更新日期:1972-05-01 00:00:00
abstract::C-TERMINALLY ENCODED PEPTIDEs (CEPs) control root system architecture in a non-cell-autonomous manner. In Medicago truncatula, MtCEP1 affects root development by increasing nodule formation and inhibiting lateral root emergence by unknown pathways. Here, we show that the MtCEP1 peptide-dependent increase in nodulation...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.16.00113
更新日期:2016-08-01 00:00:00
abstract::Maize plants (Zea mays) attacked by caterpillars release a mixture of odorous compounds that attract parasitic wasps, natural enemies of the herbivores. We assessed the genetic variability of these induced volatile emissions among 31 maize inbred lines representing a broad range of genetic diversity used by breeders i...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.104.039891
更新日期:2004-08-01 00:00:00
abstract::Upon Agrobacterium tumefaciens infection of a host plant, Tumor morphology root (Tmr) a bacterial adenosine phosphate-isopentenyltransferase (IPT), creates a metabolic bypass in the plastid for direct synthesis of trans-zeatin (tZ) with 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate as the prenyl donor. To understand ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.112.198572
更新日期:2012-07-01 00:00:00
abstract::A pyridoxal 5'-phosphate (PLP)-dependent enzyme, 1-aminocyclopropane-1-carboxylic acid (ACC) synthase (S-adenosyl-L-Met methylthioadenosine-lyase, EC 4.4.1.14), catalyzes the conversion of S-adenosyl-L-methionine (AdoMet) to ACC. A tomato ACC synthase isozyme (LE-ACS2) with a deletion of 46 amino acids at the C termin...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.121.3.913
更新日期:1999-11-01 00:00:00
abstract::The biochemical and immunochemical characterization of a superoxide dismutase (SOD, EC 1.15.1.1) from peroxisomal origin has been carried out. The enzyme is a Cu,Zn-containing SOD (CuZn-SOD) located in the matrix of peroxisomes from watermelon (Citrullus vulgaris Schrad.) cotyledons (L.M. Sandalio and L.A. del Río [19...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.108.3.1151
更新日期:1995-07-01 00:00:00
abstract::Terpenes are specialized plant metabolites that act as attractants to pollinators and as defensive compounds against pathogens and herbivores, but they also play an important role in determining the quality of horticultural food products. We show that the genome of cultivated apple (Malus domestica) contains 55 putati...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.112.208249
更新日期:2013-02-01 00:00:00
abstract::FLAGELLIN-SENSING2 (FLS2) is the plant cell surface receptor that perceives bacterial flagellin or flg22 peptide, initiates flg22-signaling responses, and contributes to bacterial growth restriction. Flg22 elicitation also leads to ligand-induced endocytosis and degradation of FLS2 within 1 h. Why plant cells remove t...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.113.229179
更新日期:2014-01-01 00:00:00
abstract::Pollen viability depends on dynamic vacuolar changes during pollen development involving increases and decreases of vacuolar volume through water and osmolite accumulation and vacuolar fission. Mutations in FAB1A to FAB1D, the genes encoding phosphatidylinositol 3,5-bisphosphate [PI(3,5)P2]-converting kinases, are mal...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.18.00495
更新日期:2018-08-01 00:00:00
abstract::The unicellular green alga Chlamydomonas reinhardtii is a widely used model organism for studies of oxygenic photosynthesis in eukaryotes. Here we describe the development of a resource for functional genomics of photosynthesis using insertional mutagenesis of the Chlamydomonas nuclear genome. Chlamydomonas cells were...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.104.055244
更新日期:2005-02-01 00:00:00
abstract::Stem sections from poplar that were stably transformed with a eucalypt cinnamyl alcohol dehydrogenase promoter-[beta]-glucuronidase construct were prepared by using either a technique routinely used in herbaceous species or a technique designed to take into account the particular anatomy of woody plants. Although both...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.113.2.321
更新日期:1997-02-01 00:00:00
abstract::The assimilation of NH(4) (+) causes a rapid increase in respiration to provided carbon skeletons for amino acid synthesis. In this study we propose a model for the regulation of carbon partitioning from starch to respiration and N assimilation in the green alga Selenastrum minutum. We provide evidence for both a cyto...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.93.1.166
更新日期:1990-05-01 00:00:00
abstract::To our knowledge, this report describes the first application of video imaging of Chl fluorescence to the study of light utilization in photosystem II of attached leaves of Phaseolus vulgaris infected with the obligate biotrophic fungus Uromyces appendiculatus (race 38). The video-based detection system produced a spa...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.108.1.163
更新日期:1995-05-01 00:00:00
abstract::Young cabbage plants (Brassica oleracea L. var. capitata) that were exposed to an atmosphere at 50% relative humidity transpired freely and accumulated significant quantities of (45)Ca in the leaves. Plants that were enclosed by plastic bags to stop transpiration from all leaves exhibited guttation with the developmen...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.60.6.854
更新日期:1977-12-01 00:00:00
abstract::Reactive oxygen species (ROS) are potent signal molecules rapidly generated in response to stress. Detection of pathogen-associated molecular patterns induces a transient apoplastic ROS through the function of the NADPH respiratory burst oxidase homologs D (RbohD). However, little is known about the regulation of path...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.110.154567
更新日期:2010-09-01 00:00:00
abstract::The field of proteomics suffers from the immense complexity of even small proteomes and the enormous dynamic range of protein concentrations within a given sample. Most protein samples contain a few major proteins, which hamper in-depth proteomic analysis. In the human field, combinatorial hexapeptide ligand libraries...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.112.198077
更新日期:2012-07-01 00:00:00
abstract::There are at least five lipoxygenases (TomloxA, TomloxB, TomloxC, TomloxD, and TomloxE) present in tomato (Lycopersicon esculentum Mill.) fruit, but their role in generation of fruit flavor volatiles has been unclear. To assess the physiological role of TomloxC in the generation of volatile C6 aldehyde and alcohol fla...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.104.041608
更新日期:2004-09-01 00:00:00
abstract::The loss of photosynthesis is frequently associated with parasitic or pathogenic lifestyles, but it also can occur in free-living, plastid-bearing lineages. A common consequence of becoming nonphotosynthetic is the reduction in size and gene content of the plastid genome. In exceptional circumstances, it can even resu...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.16.01628
更新日期:2017-02-01 00:00:00
abstract::The LOSS OF APOMEIOSIS (LOA) locus is one of two dominant loci known to control apomixis in the eudicot Hieracium praealtum. LOA stimulates the differentiation of somatic aposporous initial cells after the initiation of meiosis in ovules. Aposporous initial cells undergo nuclear proliferation close to sexual megaspore...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.111.181164
更新日期:2011-11-01 00:00:00
abstract::Changes in acyl lipids and pigments during leaf development in a virescens barley mutant (M) and the normal (N) were studied. Apical 3-cm leaf segments were extracted with chloroform-methanol, the extracts were purified on Sephadex G-25 columns, and the polar lipids were separated on two-dimensional-thin layer chromat...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.67.4.646
更新日期:1981-04-01 00:00:00
abstract::Mitochondrial citrate synthase was purified from leaves of Pisum sativum L. cv Progress 9. A three step purification was employed using ATP-Sepharose affinity chromatography which resulted in a 600-fold enrichment. Enzyme activity was assayed spectrophotometrically during greening of etiolated leaves under constant wh...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.89.3.719
更新日期:1989-03-01 00:00:00
abstract::The capacity of sodium orthovanadate to inhibit the plasmalemma H(+) ATPase of Nitella translucens internodal cells in vivo was tested. Here we show that 1 millimolar vanadate added externally depolarizes strongly and permanently the membrane potential, both in dark and light, to the Nernst potential for potassium con...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.96.1.91
更新日期:1991-05-01 00:00:00
abstract::We have isolated full-length cDNA clones that encode oat (Avena sativa) seed storage globulin mRNAs from a cDNA library in the expression vector lambda gtll. The longest of these clones, pOG2, has an 1840-base pair insert that encodes a complete precursor subunit with a signal peptide of 24 amino acids followed by an ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.87.3.698
更新日期:1988-07-01 00:00:00
abstract::Small GTP-binding proteins regulate diverse processes in eukaryotic cells such as signal transduction, cell proliferation, cytoskeletal organization, and intracellular membrane trafficking. These proteins function as molecular switches that cycle between "active" and "inactive" states, and this cycle is linked to the ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.013052
更新日期:2003-03-01 00:00:00
abstract::Vacuolar sorting receptors, BP80/VSRs, play a critical role in vacuolar trafficking of soluble proteins in plant cells. However, the mechanism of action of BP80 is not well understood. Here, we investigate the action mechanism of AtVSR1, a member of BP80 proteins in Arabidopsis (Arabidopsis thaliana), in vacuolar traf...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.110.159814
更新日期:2010-09-01 00:00:00
abstract::The effects of ultraviolet-B (UV-B) radiation on stomatal conductance (g(s)) in pea (Pisum sativum L.), commelina (Commelina communis L.), and oilseed rape (Brassica napus L.) plants were investigated. Plants were grown in a greenhouse either with three different high ratios of UV-B to photosynthetically active radiat...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.121.2.489
更新日期:1999-10-01 00:00:00
abstract::The influence of light on asexual fruiting and mycelial growth of Phycomyces blakesleeanus Burgeff was studied by means of fruiting body counts and size measurements in cultures on solid media under varied incubation conditions. Five types of photoresponses were shown by ATCC Strain 8743a: (a) photoinduction of giant ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.51.3.570
更新日期:1973-03-01 00:00:00
abstract::The carbon dioxide (CO2)-concentrating mechanism of cyanobacteria is characterized by the occurrence of Rubisco-containing microcompartments called carboxysomes within cells. The encapsulation of Rubisco allows for high-CO2 concentrations at the site of fixation, providing an advantage in low-CO2 environments. Cyanoba...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.114.237941
更新日期:2014-05-01 00:00:00
abstract::Endoplasmic reticulum membranes stripped of attached ribosomes were isolated from homogenates of germinating castor bean (Ricinus communis L.) endosperm by sucrose density gradient centrifugation. The isolated endoplasmic reticulum fraction was further separated into two major membrane subfractions by centrifugation o...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.72.2.547
更新日期:1983-06-01 00:00:00