Molecular characterization of oat seed globulins.

Abstract:

:We have isolated full-length cDNA clones that encode oat (Avena sativa) seed storage globulin mRNAs from a cDNA library in the expression vector lambda gtll. The longest of these clones, pOG2, has an 1840-base pair insert that encodes a complete precursor subunit with a signal peptide of 24 amino acids followed by an acidic polypeptide of 293 amino acids and a basic polypeptide of 201 amino acids. Near the C terminus of the acidic polypeptide are four repeats of a highly conserved, glutamine-rich octapeptide. Other oat globulin cDNA clones contain five of these repeats. Nucleotide sequence comparisons between these clones indicate that the genes encoding these proteins are highly conserved. We estimate there to be 7 to 10 genes for the oat globulin per haploid genome. Comparisons of amino acid sequences show that the oat globulin is 30 to 40% homologous with storage globulins of legumes and about 70% homologous with the rice seed storage globulin (glutelin).

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Shotwell MA,Afonso C,Davies E,Chesnut RS,Larkins BA

doi

10.1104/pp.87.3.698

subject

Has Abstract

pub_date

1988-07-01 00:00:00

pages

698-704

issue

3

eissn

0032-0889

issn

1532-2548

journal_volume

87

pub_type

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