Abstract:
:This study was performed to test if alternative carbon sources besides recently photosynthetically fixed CO2 are used for isoprene formation in the leaves of young poplar (Populus x canescens) trees. In a 13CO2 atmosphere under steady state conditions, only about 75% of isoprene became 13C labeled within minutes. A considerable part of the unlabeled carbon may be derived from xylem transported carbohydrates, as may be shown by feeding leaves with [U-13C]Glc. As a consequence of this treatment approximately 8% to 10% of the carbon emitted as isoprene was 13C labeled. In order to identify further carbon sources, poplar leaves were depleted of leaf internal carbon pools and the carbon pools were refilled with 13C labeled carbon by exposure to 13CO2. Results from this treatment showed that about 30% of isoprene carbon became 13C labeled, clearly suggesting that, in addition to xylem transported carbon and CO2, leaf internal carbon pools, e.g. starch, are used for isoprene formation. This use was even increased when net assimilation was reduced, for example by abscisic acid application. The data provide clear evidence of a dynamic exchange of carbon between different cellular precursors for isoprene biosynthesis, and an increasing importance of these alternative carbon pools under conditions of limited photosynthesis. Feeding [1,2-13C]Glc and [3-13C]Glc to leaves via the xylem suggested that alternative carbon sources are probably derived from cytosolic pyruvate/phosphoenolpyruvate equivalents and incorporated into isoprene according to the predicted cleavage of the 3-C position of pyruvate during the initial step of the plastidic deoxyxylulose-5-phosphate pathway.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Schnitzler JP,Graus M,Kreuzwieser J,Heizmann U,Rennenberg H,Wisthaler A,Hansel Adoi
10.1104/pp.103.037374subject
Has Abstractpub_date
2004-05-01 00:00:00pages
152-60issue
1eissn
0032-0889issn
1532-2548pii
pp.103.037374journal_volume
135pub_type
杂志文章相关文献
PLANT PHYSIOLOGY文献大全abstract::The Arabidopsis (Arabidopsis thaliana) root epidermal bulger1-1 (reb1-1) mutant (allelic to root hair defective1 [rhd1]) is characterized by a reduced root elongation rate and by bulging of trichoblast cells. The REB1/RHD1 gene belongs to a family of UDP-D-Glucose 4-epimerases involved in the synthesis of D-Galactose ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.105.074997
更新日期:2006-04-01 00:00:00
abstract::Arabidopsis (Arabidopsis thaliana) leaf development relies on subsequent phases of cell proliferation and cell expansion. During the proliferation phase, chloroplasts need to divide extensively, and during the transition from cell proliferation to expansion, they differentiate into photosynthetically active chloroplas...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.114.256180
更新日期:2015-03-01 00:00:00
abstract::Polyphosphoinositide-specific phospholipase C activity was present in plasma membranes isolated from different tissues of several higher plants. Phospholipase C activities against added phosphatidylinositol 4-phosphate (PIP) and phosphatidylinositol 4,5-bisphosphate (PIP(2)) were further characterized in plasma membra...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.100.3.1296
更新日期:1992-11-01 00:00:00
abstract::Here, we report the aluminum (Al)-induced organ-specific expression of a WAK1 (cell wall-associated receptor kinase 1) gene and cell type-specific localization of WAK proteins in Arabidopsis. WAK1-specific reverse transcriptase-polymerase chain reaction analysis revealed an Al-induced WAK1 gene expression in roots. Sh...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.103.022129
更新日期:2003-08-01 00:00:00
abstract::Plant cytosolic lipid droplets (LDs) are covered with a layer of phospholipids and oleosin and were extensively studied before those in mammals and yeast. Oleosin has short amphipathic N- and C-terminal peptides flanking a conserved 72-residue hydrophobic hairpin, which penetrates and stabilizes the LD Oleosin is synt...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.17.00366
更新日期:2017-08-01 00:00:00
abstract::Epidemics of obesity and type 2 diabetes drive strong consumer interest in plant-based low-calorie sweeteners. Trilobatin is a sweetener found at high concentrations in the leaves of a range of crabapple (Malus) species, but not in domesticated apple (Malus × domestica) leaves, which contain trilobatin's bitter positi...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.20.00807
更新日期:2020-10-01 00:00:00
abstract::Major storage reserves of Arabidopsis (Arabidopsis thaliana) seeds are triacylglycerols (seed oils) and proteins. Seed oil content is severely reduced for the regulatory mutant wrinkled1 (wri1-1; At3g54320) and for a double mutant in two isoforms of plastidic pyruvate kinase (pkpbeta(1)pkpalpha; At5g52920 and At3g2296...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.109.144121
更新日期:2009-11-01 00:00:00
abstract::Stationary volume fluxes through living and denatured parenchyma slices of the potato (Solanum tuberosum L.) storage organ were studied to estimate the hydraulic conductivity of the cell wall and to evaluate the significance of water transport through protoplasts, cell walls, and intercellular spaces. Slices were plac...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.115.3.1089
更新日期:1997-11-01 00:00:00
abstract::Maize (Zea mays L.) endosperm suspension cultures are a useful model system for studying biochemical and physiological events in developing maize endosperm. In this report, sugar uptake by the cultures is characterized. Uptake of (14)C-labeled fructose and l-glucose was linear with time, while the rate of uptake of ra...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.88.4.1235
更新日期:1988-12-01 00:00:00
abstract::The radial movement of oxygen in excised corn and jack bean roots was measured with a platinum wire electrode embedded in the root tissue. Measurements were made with the roots exposed to air and with the roots immersed in nutrient solution in the presence and absence of millimolar sodium azide. Effective rates of oxy...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.45.6.667
更新日期:1970-06-01 00:00:00
abstract::We have determined the levels of photosystem II activity and polypeptide abundance in whole leaves and isolated bundle sheath and mesophyll cells of C(4), "C(4)-like," and C(3) species of the genus Flaveria (Asteraceae). On a chlorophyll basis, the whole leaf levels of the D1, D2, and 34-kilodalton photosystem II poly...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.98.3.1154
更新日期:1992-03-01 00:00:00
abstract::The effect of ethylene on the growth rate of pollen tubes in styles of Petunia hybrida was examined. Apart from its strong inhibition of pollination-induced ethylene synthesis, aminoethoxyvinylglycine, placed on the stigma, did not impede tube growth. The inhibitors of the action of ethylene, silver thiosulfate and 2,...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.86.1.4
更新日期:1988-01-01 00:00:00
abstract::Trithorax group proteins are chromatin-remodeling factors that activate target gene expression by antagonistically functioning against the Polycomb group. In Arabidopsis (Arabidopsis thaliana), Arabidopsis Trithorax protein1 (ATX1) regulates flowering time and floral organ identity. Here, we observed that suppression ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.113.228049
更新日期:2014-03-01 00:00:00
abstract::Disc electrophoresis was used to separate water soluble proteins from hardy, non-hardy, and frost killed cabbage (Brassica oleracea var. capitata) leaves. Amidoschwarz staining failed to reveal any new bands as a result of hardening although the relative amounts of proteins in individual bands changed. Sulfhydryl grou...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.44.2.168
更新日期:1969-02-01 00:00:00
abstract::Phototropism and hypocotyl growth inhibition are modulated by the coaction of different blue-light photoreceptors and their signaling pathways. How seedlings integrate the activities of the different blue-light photoreceptors to coordinate these hypocotyl growth responses is still unclear. We have used time-lapse imag...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.102.018481
更新日期:2003-07-01 00:00:00
abstract::Nuclear pore complexes (NPCs) mediate the transport of RNA and other cargo between the nucleus and the cytoplasm. In vertebrates, the NPC protein TRANSLOCATED PROMOTER REGION (TPR) is associated with the inner filaments of the nuclear basket and is thought to serve as a scaffold for the assembly of transport machinery...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.107.100735
更新日期:2007-07-01 00:00:00
abstract::Adenosine kinase was partially purified from wheat germ. This enzyme preparation, which was devoid of adenine phosphoribosyltransferase and nearly free of adenosine deaminase but contained adenylate kinase, rapidly phosphorylated adenosine and a cytokinin, N(6)-(delta(2)-isopentenyl)adenosine. Electrophoretic analysis...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.59.3.443
更新日期:1977-03-01 00:00:00
abstract::Amyloplasts of starchy tissues such as those of maize (Zea mays L.) function in the synthesis and accumulation of starch during kernel development. ADP-glucose pyrophosphorylase (AGPase) is known to be located in chloroplasts, and for many years it was generally accepted that AGPase was also localized in amyloplasts o...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.117.4.1235
更新日期:1998-08-01 00:00:00
abstract::4,6-Dioxoheptanoic acid (DA), an inhibitor of 5-aminolevulinic acid (ALA) dehydratase (EC 4.3.1.24), causes ALA to accumulate at the expense of chlorophyll when applied to greening leaves of Hordeum vulgare L. var. Larker. Preincubating etiolated leaves with DA in darkness eliminates the lag phase in ALA accumulation ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.67.6.1065
更新日期:1981-06-01 00:00:00
abstract::A metabolite profiling technique for Chlamydomonas reinhardtii cells for multiparallel analysis of low-molecular weight polar compounds was developed. The experimental protocol was optimized to quickly inactivate enzymatic activity, achieve maximum extraction capacity, and process large sample quantities. As a result ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.105.071589
更新日期:2005-12-01 00:00:00
abstract::The plant plasma membrane H(+)-ATPase energizes the secondary uptake of nutrients and may facilitate cell expansion by acidifying the cell wall. In yeast, Glc stimulates the accumulation of H(+)-ATPase mRNA, and the growth rate supported by various sugars is correlated with H(+)-ATPase protein abundance. Expression of...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.112.3.1229
更新日期:1996-11-01 00:00:00
abstract::Cyclic nucleotide-gated channels (CNGCs) have been implicated in diverse aspects of plant growth and development, including responses to biotic and abiotic stress, as well as pollen tube growth and fertility. Here, genetic evidence identifies CNGC16 in Arabidopsis (Arabidopsis thaliana) as critical for pollen fertilit...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.112.206888
更新日期:2013-02-01 00:00:00
abstract::A Mg(2+)-dependent, cation-stimulated ATPase was associated with plasma membranes isolated from corn leaf mesophyll protoplasts. Potassium was the preferred monovalent cation for stimulating the ATPase above the Mg(2+)-activated level. The enzyme was substrate-specific for ATP, was inhibited by N,N'-dicyclohexylcarbod...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.68.3.521
更新日期:1981-09-01 00:00:00
abstract::A mutant of Arabidopsis thaliana has been isolated in which ribulose-1,5-bisphosphate carboxylase is present in a nonactivatable form in vivo. The mutation appears to affect carboxylase activation specifically, and not any other enzyme of the photosynthesis or photorespiratory cycles. The effect of the mutation on car...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.70.2.381
更新日期:1982-08-01 00:00:00
abstract::The soluble proteins of C(3) and C(4) mesophyll chloroplasts and C(4) bundle sheath extracts have been analyzed by gel electrophoresis for fraction I protein. Gel scans of soluble protein from C(4) bundle sheath extracts and C(3) mesophyll chloroplasts showed typical fraction I protein peaks that could be identified b...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.57.5.730
更新日期:1976-05-01 00:00:00
abstract::Fruit ripening and abscission are associated with an ethylene burst in several melon (Cucumis melo) genotypes. In cantaloupe as in other climacteric fruit, exogenous ethylene can prematurely induce abscission, ethylene production, and ripening. Melon genotypes without fruit abscission or without ethylene burst also ex...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.010613
更新日期:2002-05-01 00:00:00
abstract::In this study, we develop a mechanistic understanding of how temperature affects growth and photosynthesis in 10 geographically and physiologically diverse strains of Synechococcus spp. We found that Synechococcus spp. are able to regulate photochemistry over a range of temperatures by using state transitions and alte...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.113.221937
更新日期:2013-10-01 00:00:00
abstract::Cell-free preparations from seeds of Marah macrocarpus L. and Malus domestica L. catalyzed the conversion of gibberellin A(9) (GA(9)) and 2,3-dehydroGA(9) to GA(7); GA(9) was also metabolized to GA(4) in a branch pathway. The preparation from Marah seeds also metabolized GA(5) to GA(3) in high yield; GA(6) was a minor...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.94.1.132
更新日期:1990-09-01 00:00:00
abstract::A technique of centrifuging pea epicotyl sections which extracts water-soluble cell wall polysaccharides with less than 1.5% cytoplasmic contamination as revealed by malate dehydrogenase activity determinations was developed. Tests for protein, hexose, pentose, and malate dehydrogenase indicate that significant damage...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.66.2.321
更新日期:1980-08-01 00:00:00
abstract::The activity of the alternative pathway is affected by a number of factors, including the level and reduction state of the alternative oxidase (AOX) protein, and the reduction state of the ubiquinone pool. To investigate the significance of these factors for the rate of alternative respiration in vivo, we studied root...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.126.1.376
更新日期:2001-05-01 00:00:00