The liverwort contains a lectin that is structurally and evolutionary related to the monocot mannose-binding lectins.

Abstract:

:A mannose (Man)-binding lectin has been isolated and characterized from the thallus of the liverwort Marchantia polymorpha. N-terminal sequencing indicated that the M. polymorpha agglutinin (Marpola) shares sequence similarity with the superfamily of monocot Man-binding lectins. Searches in the databases yielded expressed sequence tags encoding Marpola. Sequence analysis, molecular modeling, and docking experiments revealed striking structural similarities between Marpola and the monocot Man-binding lectins. Activity and specificity studies further indicated that Marpola is a much stronger agglutinin than the Galanthus nivalis agglutinin and exhibits a preference for methylated Man and glucose, which is unprecedented within the family of monocot Man-binding lectins. The discovery of Marpola allows us, for the first time, to corroborate the evolutionary relationship between a lectin from a lower plant and a well-established lectin family from flowering plants. In addition, the identification of Marpola sheds a new light on the molecular evolution of the superfamily of monocot Man-binding lectins. Beside evolutionary considerations, the occurrence of a G. nivalis agglutinin homolog in a lower plant necessitates the rethinking of the physiological role of the whole family of monocot Man-binding lectins.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Peumans WJ,Barre A,Bras J,Rougé P,Proost P,Van Damme EJ

doi

10.1104/pp.010959

subject

Has Abstract

pub_date

2002-07-01 00:00:00

pages

1054-65

issue

3

eissn

0032-0889

issn

1532-2548

journal_volume

129

pub_type

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