Solubilization, Partial Purification, and Characterization of a Binding Site for a Glycopeptide Elicitor from Microsomal Membranes of Tomato Cells.

abstract：:The plant hormone jasmonic acid (JA) plays a pivotal role in plant-insect interactions. Herbivore attack usually elicits dramatic increases in JA concentrations, which in turn activate the accumulation of metabolites that function as defenses against herbivores. Although almost all enzymes involved in the biosynthesis...

journal_title：Plant physiology

authors： Yang DH,Hettenhausen C,Baldwin IT,Wu J

更新日期：2012-08-01 00:00:00

abstract：:Starch, total amylolytic and phosphorylase activities were determined in lentil cotyledons during the first days of germination. Several independent criteria show that the amylolytic activity is due mainly to an amylase of the alpha type. Starch is degraded slowly in the first days; during this time, alpha- and beta-a...

journal_title：Plant physiology

authors： Tárrago JF,Nicolás G

更新日期：1976-11-01 00:00:00

abstract：:We have found that NPR1 and NPR2, two genes from Lemna gibba L. G-3 that can be negatively regulated by phytochrome action, can also be positively regulated by the plant hormone abscisic acid (ABA). Both genes were responsive to low concentrations of exogenous ABA; an increase in NPR1 RNA could be detected in response...

journal_title：Plant physiology

authors： Williams SA,Weatherwax SC,Bray EA,Tobin EM

更新日期：1994-07-01 00:00:00

abstract：:Detergent-solubilized plasma membrane proteins from pea (Pisum sativum L.) stem tissue were separated by isoelectric focusing (IEF) using a Bio-Rad Rotofor cell, with the goal of identifying protein(s) involved in beta-1,3-glucan synthase (GS-II) activity. Ordinary IEF procedures result in membrane protein precipitati...

journal_title：Plant physiology

authors： Dhugga KS,Ray PM

更新日期：1991-04-01 00:00:00

abstract：:Reduction of Cu2+ by plasma membrane vesicles isolated from tomato (Lycopersicon esculentum Mill.) roots was investigated. Plants were grown in hydroponic culture with complete nutrition for 4 weeks or were deprived of Fe for the last 7 d. Plasma membrane vesicles were prepared by aqueous two-phase partitioning. Reduc...

journal_title：Plant physiology

authors： Holden MJ,Crimmins TJ Jr,Chaney RL

更新日期：1995-07-01 00:00:00

abstract：:Analysis of inner and outer pea (Pisum sativum var. Laxtons Progress No. 9) chloroplast envelope membranes by sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed that, although the two membranes have distinct polypeptide compositions, there are several comigrating polypeptides in the two membrane fracti...

journal_title：Plant physiology

authors： Werner-Washburne M,Cline K,Keegstra K

更新日期：1983-11-01 00:00:00

abstract：:The mode of abscisic acid (ABA) uptake was studied in excised leaf and root tissue discs of sugar beet (Beta vulgaris L.). Discs were incubated in buffered medium that contained 1 mm CaCl(2) and [(14)C]ABA. The sensitivity of ABA uptake to metabolic inhibitors and temperature indicated that the ABA transport system ha...

journal_title：Plant physiology

authors： Daie J,Wyse R

更新日期：1983-06-01 00:00:00

abstract：:Iron (Fe) is an essential mineral nutrient and an important factor for the composition of natural plant communities. Low Fe availability in aerated soils with neutral or alkaline pH has led to the evolution of elaborate mechanisms that extract Fe from the soil solution. In Arabidopsis (Arabidopsis thaliana), Fe is acq...

journal_title：Plant physiology

authors： Tsai HH,Rodríguez-Celma J,Lan P,Wu YC,Vélez-Bermúdez IC,Schmidt W

更新日期：2018-05-01 00:00:00

abstract：:The pigment content of dark-grown primary needles of Pinus jeffreyi L. and Pinus sylvestris L. was determined by high-performance liquid chromatography. The state of protochlorophyllide a and of chlorophylls during dark growth were analyzed by in situ 77 K fluorescence spectroscopy. Both measurements unambiguously dem...

journal_title：Plant physiology

authors： Schoefs B,Franck F

更新日期：1998-12-01 00:00:00

abstract：:We report the molecular characterization and functional analysis of a gene (PsdeltaOAT) from Scots pine (Pinus sylvestris) encoding Orn-delta-aminotransferase (delta-OAT; EC 2.6.1.13), an enzyme of arginine metabolism. The deduced amino acid sequence contains a putative N-terminal signal peptide for mitochondrial targ...

journal_title：Plant physiology

authors： Cañas RA,Villalobos DP,Díaz-Moreno SM,Cánovas FM,Cantón FR

更新日期：2008-09-01 00:00:00

abstract：:As regulators of gene expression in multicellular organisms, microRNAs (miRNAs) are crucial for growth and development. Although a plethora of factors involved in their biogenesis and action in Arabidopsis (Arabidopsis thaliana) has been described, these processes and their fine-tuning are not fully understood. Here, ...

journal_title：Plant physiology

authors： Lang PLM,Christie MD,Dogan ES,Schwab R,Hagmann J,van de Weyer AL,Scacchi E,Weigel D

更新日期：2018-01-01 00:00:00

abstract：:Light-induced modifications in lipoxidase metabolism and chlorophyll formation in the cotyledon of squash (Cucurbita moscata) seedlings were determined. The enzyme activity decreased as light intensity increased, but chlorophyll continued to accumulate long after lipoxidase activity had virtually disappeared. Consider...

journal_title：Plant physiology

authors： Surrey K,Barr EM

更新日期：1966-05-01 00:00:00

abstract：:Cultures of Lemna gibba L. G3 were maintained at a constant, N-limited growth rate by adding nitrate daily in amounts calculated to sustain a rate of culture N increment of 0.20 day(-1). Nitrate added to the culture was consumed within 8 to 10 hours and the partitioning to reduction and accumulation during this phase ...

journal_title：Plant physiology

authors： Ingemarsson B

更新日期：1987-11-01 00:00:00

abstract：:Increased ethylene synthesis was rapidly induced throughout the apical meristematic region of etiolated seedlings of Pisum sativum L., cv. Alaska by cuts made 1 centimeter from the apical hook. The wound signal was transmitted at about 2 millimeters per minute. Accumulation of substance(s) at the cut surfaces of excis...

journal_title：Plant physiology

authors： Saltveit ME,Dilley DR

更新日期：1978-11-01 00:00:00

abstract：:Plants commonly live in a symbiotic association with arbuscular mycorrhizal fungi (AMF). They invest photosynthetic products to feed their fungal partners, which, in return, provide mineral nutrients foraged in the soil by their intricate hyphal networks. Intriguingly, AMF can link neighboring plants, forming common m...

journal_title：Plant physiology

authors： Walder F,Niemann H,Natarajan M,Lehmann MF,Boller T,Wiemken A

更新日期：2012-06-01 00:00:00

abstract：:Membranes enriched in ATP-dependent proton transport were prepared from suspension cultures of tomato cells (Lycopersicon esculentum Mill cv VF36). Suspension cultures were a source of large quantities of membranes from rapidly growing, undifferentiated cells. Proton transport activity was assayed as quench of acridin...

journal_title：Plant physiology

authors： Dupont FM,Zabala Mde G

更新日期：1985-01-01 00:00:00

abstract：:It has been reported that hydroxyphenylethylamines, such as tyramine and octopamine, are toxic to tobacco (Nicotiana tabacum L.) callus cultures grown in the presence of auxins, whereas calli grown in the presence of cytokinins and crown gall cultures are resistant to these amines (P. Christou and K.A. Barton [1989] P...

journal_title：Plant physiology

authors： Negrel J,Javelle F,Paynot M

更新日期：1993-10-01 00:00:00

abstract：:Cold hardiness of leaf blades, leaf sheaths, culms, rhizomes, and leaf buds in wintering Sasa senanensis (Fr. et Sav.) Rehder, a dwarf bamboo, was studied paying special attention to the types of resistance mechanisms which were determined with differential thermal analysis. Coincidence of LT(25) (lethal temperature a...

journal_title：Plant physiology

authors： Ishikawa M

更新日期：1984-05-01 00:00:00

abstract：:Glycoprotein nonspecific elicitor (NSE) and a specific elicitor preparation from intercellular fluids (SE) of tomato (Lycopersicon esculentum Mill. cv Bonny Best or Potentate) infected with race 2.4.5 of Cladosporium fulvum Cooke [syn. Fulvia fulva (Cooke) Ciferri] were injected into cv Sonatine (resistant to race 2.4...

journal_title：Plant physiology

authors： Peever TL,Higgins VJ

更新日期：1989-07-01 00:00:00

abstract：:ZRP4, a 1.4-kb mRNA that preferentially accumulates in roots of young Zea mays L. plants, was identified by isolation of the corresponding cDNA clone. Genomic Southern analysis indicates that the zrp4 gene is represented once in the corn genome. The deduced ZRP4 polypeptide of 39,558 D is rich in leucine, serine, and ...

journal_title：Plant physiology

authors： Held BM,Wang H,John I,Wurtele ES,Colbert JT

更新日期：1993-07-01 00:00:00

abstract：:A procedure is described for isolating and purifying mesophyll protoplasts and bundle sheath protoplasts of the C(4) plant Panicum miliaceum. Following enzymic digestion of leaf tissue, mesophyll protoplasts and bundle sheath protoplasts are released and purified by density centrifugation. The lower density of mesophy...

journal_title：Plant physiology

authors： Edwards GE

更新日期：1979-05-01 00:00:00

abstract：:Promoters of most seed proteins in legumes contain one or more 5'-CATGCAT-3' elements. To test if these elements have a function in the expression of these genes, the 2.3 kilobase pairs Gy2 glycinin promoter was ligated to a beta-glucuronidase reporter sequence and transformed into tobacco. Elimination of a 5'-CATGCAT...

journal_title：Plant physiology

authors： Lelievre JM,Oliveira LO,Nielsen NC

更新日期：1992-01-01 00:00:00

abstract：:We compared the subcellular distribution of native and artificial reticuloplasmins in endosperm, callus, and leaf tissues of transgenic rice (Oryza sativa) to determine the distribution of these proteins among endoplasmic reticulum (ER) and post-ER compartments. The native reticuloplasmin was calreticulin. The artific...

journal_title：Plant physiology

authors： Torres E,Gonzalez-Melendi P,Stöger E,Shaw P,Twyman RM,Nicholson L,Vaquero C,Fischer R,Christou P,Perrin Y

更新日期：2001-11-01 00:00:00

abstract：:Cysthathionine [gamma]-synthase (CS), an enzyme involved in methionine biosynthesis, was purified from an acetone powder prepared from wheat (Triticum aestivum L.). After several chromatographic steps and radiolabeling of the partially purified enzyme with sodium cyanoboro[3H]hydride, a single polypeptide with a molec...

journal_title：Plant physiology

authors： Kreft BD,Townsend A,Pohlenz HD,Laber B

更新日期：1994-04-01 00:00:00

abstract：:The ripening of a fleshy fruit represents the summation of an array of biochemical processes that are regulated by interactions between developmental programs and environmental inputs. Analysis of tomato (Solanum lycopersicum) mutants and inhibitor studies indicate that ethylene is necessary for full development of th...

journal_title：Plant physiology

authors： Barry CS,McQuinn RP,Thompson AJ,Seymour GB,Grierson D,Giovannoni JJ

更新日期：2005-05-01 00:00:00

abstract：:The mitochondrial ATP-binding cassette transporter ATM3 has been studied in Arabidopsis. Its function, however, is poorly understood in other model plant species. This study reports that the ATM3 is required for cytosolic iron-sulfur cluster assembly and is essential for meristem maintenance in rice (Oryza sativa). Th...

journal_title：Plant physiology

authors： Zuo J,Wu Z,Li Y,Shen Z,Feng X,Zhang M,Ye H

更新日期：2017-04-01 00:00:00

abstract：:The primary cell walls of graminaceous monocots were known to have a low content of pectin compared to those of dicots, but it was uncertain how widespread this feature was within the monocots as a whole. Nonlignified cell walls were therefore prepared from 33 monocot species for determination of their pectin content....

journal_title：Plant physiology

authors： Jarvis MC,Forsyth W,Duncan HJ

更新日期：1988-10-01 00:00:00

abstract：:Bicarbonate ion, not dissolved CO(2) gas, is shown to increase 4- to 5-fold the rate of dichlorophenol indophenol reduction by isolated maize (Zea mays) chloroplasts. Glutaraldehyde fixed chloroplasts continue to exhibit bicarbonate-dependent 2,6-dichlorophenol indophenol reduction. Bicarbonate is shown to act close t...

journal_title：Plant physiology

authors： Stemler A,Govindjee

更新日期：1973-08-01 00:00:00

abstract：:Spontaneous growth of isolated inner tissue from the etiolated sunflower (Helianthus annuus L.) hypocotyl growing zone was investigated. A new preparation technique allowed measurements starting 3 s after excision. Elongation with respect to the turgescent and plasmolized state was quantified in terms of relative grow...

journal_title：Plant physiology

authors： Peters WS,Tomos AD

更新日期：2000-06-01 00:00:00

abstract：:Cereal kernel growth and grain yield are functions of endosperm starch accumulation. The objective of this study was to examine how various metabolic factors in developing maize (Zea mays L.) endosperm influence starch deposition. Kernels were grown in vitro on medium with: (a) zero N (-N), (b) optimum N (+N), or (c) ...

journal_title：Plant physiology

authors： Singletary GW,Below FE

更新日期：1990-01-01 00:00:00