NPR genes, which are negatively regulated by phytochrome action in Lemna gibba L. G-3, can also be positively regulated by abscisic acid.

Abstract:

:We have found that NPR1 and NPR2, two genes from Lemna gibba L. G-3 that can be negatively regulated by phytochrome action, can also be positively regulated by the plant hormone abscisic acid (ABA). Both genes were responsive to low concentrations of exogenous ABA; an increase in NPR1 RNA could be detected in response to concentrations as low as 10 nM. We have also tested phytochrome responsiveness of 5' promoter-deletion constructs of one of these genes, NPR1, in transient assays utilizing particle bombardment. This analysis demonstrated that DNA sequences important for phytochrome regulation are present downstream of -198 from the transcription start site. A response to ABA treatment could also be observed in the transient assay system. When intact plants were placed in darkness, there was an increase in ABA levels as well as increased levels of NPR1 and NPR2 RNA.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Williams SA,Weatherwax SC,Bray EA,Tobin EM

doi

10.1104/pp.105.3.949

subject

Has Abstract

pub_date

1994-07-01 00:00:00

pages

949-54

issue

3

eissn

0032-0889

issn

1532-2548

pii

105/3/949

journal_volume

105

pub_type

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