Abstract:
:We have found that NPR1 and NPR2, two genes from Lemna gibba L. G-3 that can be negatively regulated by phytochrome action, can also be positively regulated by the plant hormone abscisic acid (ABA). Both genes were responsive to low concentrations of exogenous ABA; an increase in NPR1 RNA could be detected in response to concentrations as low as 10 nM. We have also tested phytochrome responsiveness of 5' promoter-deletion constructs of one of these genes, NPR1, in transient assays utilizing particle bombardment. This analysis demonstrated that DNA sequences important for phytochrome regulation are present downstream of -198 from the transcription start site. A response to ABA treatment could also be observed in the transient assay system. When intact plants were placed in darkness, there was an increase in ABA levels as well as increased levels of NPR1 and NPR2 RNA.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Williams SA,Weatherwax SC,Bray EA,Tobin EMdoi
10.1104/pp.105.3.949subject
Has Abstractpub_date
1994-07-01 00:00:00pages
949-54issue
3eissn
0032-0889issn
1532-2548pii
105/3/949journal_volume
105pub_type
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