Phytochrome- and gibberellin-mediated regulation of abscisic acid metabolism during germination of photoblastic lettuce seeds.


:Germination of lettuce (Lactuca sativa) 'Grand Rapids' seeds is regulated by phytochrome. The action of phytochrome includes alterations in the levels of gibberellin (GA) and abscisic acid (ABA). To determine the molecular mechanism of phytochrome regulation of ABA metabolism, we isolated four lettuce cDNAs encoding 9-cis-epoxycarotenoid dioxygenase (biosynthesis; LsNCED1-LsNCED4) and four cDNAs for ABA 8'-hydroxylase (catabolism; LsABA8ox1-LsABA8ox4). Measurements of ABA and its catabolites showed that a decrease in ABA level coincided with a slight increase in the level of the ABA catabolite phaseic acid after red light treatment. Quantitative reverse transcription-polymerase chain reaction analysis indicated that ABA levels are controlled by phytochrome through down-regulation of LsNCED2 and LsNCED4 expression and up-regulation of LsABA8ox4 expression in lettuce seeds. Furthermore, the expression levels of LsNCED4 decreased after GA(1) treatment, whereas the levels of expression of the other two genes were unaffected. The LsNCED4 expression was also down-regulated by red light in lettuce seeds in which GA biosynthesis was suppressed by AMO-1618, a specific GA biosynthesis inhibitor. These results indicate that phytochrome regulation of ABA metabolism is mediated by both GA-dependent and -independent mechanisms. Spatial analysis showed that after red light treatment, the ABA decrease on the hypocotyl side was greater than that on the cotyledon side of lettuce seeds. Moreover, phytochrome-regulated expression of ABA and GA biosynthesis genes was observed on the hypocotyl side, rather than the cotyledon side, suggesting that this regulation occurs near the photoperceptive site.


Plant Physiol


Plant physiology


Sawada Y,Aoki M,Nakaminami K,Mitsuhashi W,Tatematsu K,Kushiro T,Koshiba T,Kamiya Y,Inoue Y,Nambara E,Toyomasu T




Has Abstract


2008-03-01 00:00:00














  • Buoyant density studies of chloroplast and nuclear deoxyribonucleic acid from control and 3-amino-1,2,4-triazole-treated wheat seedlings, Triticum vulgare.

    abstract::The isolation of chloroplast and nuclear DNA from dark- and light-grown, control- and 3-amino-1,2,4-triazole-treated wheat seedlings, Triticum vulgare, is described. Contrary to a previous report, we found that chloroplast and nuclear DNA had similar buoyant densities (1.702 grams per cubic centimeter) and that they c...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Bartels PG,Hyde A

    更新日期:1970-12-01 00:00:00

  • Effect of polar lipids on ATPase activity of membrane preparations from germinating radish seeds.

    abstract::Membrane preparation (sedimenting between 13,000(g) and 80,000(g)) of germinating radish seeds (Raphanus sativus L.) was active in hydrolyzing ATP and, to a lesser extent, a variety of other phosphorylated compounds. Dicyclohexylcarbodiimide (DCCD) and diethylstilbestrol significantly inhibited the ATPase activity (40...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Cocucci M,Ballarin-Denti A

    更新日期:1981-08-01 00:00:00

  • A Common histone modification code on C4 genes in maize and its conservation in Sorghum and Setaria italica.

    abstract::C4 photosynthesis evolved more than 60 times independently in different plant lineages. Each time, multiple genes were recruited into C4 metabolism. The corresponding promoters acquired new regulatory features such as high expression, light induction, or cell type-specific expression in mesophyll or bundle sheath cell...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Heimann L,Horst I,Perduns R,Dreesen B,Offermann S,Peterhansel C

    更新日期:2013-05-01 00:00:00

  • Fatty acid activation in cyanobacteria mediated by acyl-acyl carrier protein synthetase enables fatty acid recycling.

    abstract::In cyanobacteria fatty acids destined for lipid synthesis can be synthesized de novo, but also exogenous free fatty acids from the culture medium can be directly incorporated into lipids. Activation of exogenous fatty acids is likely required prior to their utilization. To identify the enzymatic activity responsible f...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Kaczmarzyk D,Fulda M

    更新日期:2010-03-01 00:00:00

  • A developmental transcriptional network for maize defines coexpression modules.

    abstract::Here, we present a genome-wide overview of transcriptional circuits in the agriculturally significant crop species maize (Zea mays). We examined transcript abundance data at 50 developmental stages, from embryogenesis to senescence, for 34,876 gene models and classified genes into 24 robust coexpression modules. Modul...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Downs GS,Bi YM,Colasanti J,Wu W,Chen X,Zhu T,Rothstein SJ,Lukens LN

    更新日期:2013-04-01 00:00:00

  • Cytoplasmic pH dynamics in maize pulvinal cells induced by gravity vector changes.

    abstract::In maize (Zea mays) and other grasses, changes in orientation of stems are perceived by pulvinal tissue, which responds to the stimulus by differential growth resulting in upward bending of the stem. The amyloplast-containing bundle sheath cells are the sites of gravity perception, although the initial steps of gravit...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Johannes E,Collings DA,Rink JC,Allen NS

    更新日期:2001-09-01 00:00:00

  • Rapidly Induced Wound Ethylene from Excised Segments of Etiolated Pisum sativum L., cv. Alaska: III. Induction and Transmission of the Response.

    abstract::Increased ethylene synthesis was rapidly induced throughout the apical meristematic region of etiolated seedlings of Pisum sativum L., cv. Alaska by cuts made 1 centimeter from the apical hook. The wound signal was transmitted at about 2 millimeters per minute. Accumulation of substance(s) at the cut surfaces of excis...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Saltveit ME,Dilley DR

    更新日期:1978-11-01 00:00:00

  • Chilling-Induced Lipid Degradation in Cucumber (Cucumis sativa L. cv Hybrid C) Fruit.

    abstract::Chilling at 4 degrees C in the dark induced lipid degradation in cucumber (Cucumis sativa L.) fruit upon rewarming at 14 degrees C. Rates of ethane evolution by fruits rewarmed after 3 days of chilling were up to four-fold higher than those evolved by unchilled (14 degrees C) fruits (0.02-0.05 picomoles gram fresh wei...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Parkin KL,Kuo SJ

    更新日期:1989-07-01 00:00:00

  • Separation of Chlorophyll Degradation from Other Senescence Processes in Leaves of a Mutant Genotype of Meadow Fescue (Festuca pratensis L.).

    abstract::Chlorophyll levels in l-cm sections of the youngest fully expanded leaves of normal (Y) Festuca pratensis L. declined almost to zero over a period of 6 days after excision. Chlorophyll in a mutant genotype (NY) remained near the initial level for the whole of this period. Abscisic acid promoted pigment loss in Y but h...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Thomas H,Stoddart JL

    更新日期:1975-09-01 00:00:00

  • Starch degradation in the cotyledons of germinating lentils.

    abstract::Starch, total amylolytic and phosphorylase activities were determined in lentil cotyledons during the first days of germination. Several independent criteria show that the amylolytic activity is due mainly to an amylase of the alpha type. Starch is degraded slowly in the first days; during this time, alpha- and beta-a...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Tárrago JF,Nicolás G

    更新日期:1976-11-01 00:00:00

  • Induction of reduced photorespiratory activity in submersed and amphibious aquatic macrophytes.

    abstract::Incubation under water in a 30 C/14-hour or 12 C/10-hour photoperiod caused the CO(2) compensation points of 10 aquatic macrophytes to decrease below 25 or increase above 50 microliters CO(2) per liter, respectively. Submerged and aerial leaves of two amphibious angiosperms (Myriophyllum brasiliense and Proserpinaca p...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Salvucci ME,Bowes G

    更新日期:1981-02-01 00:00:00

  • Identification and Partial Characterization of the Denaturation Transition of the Photosystem II Reaction Center of Spinach Chloroplast Membranes.

    abstract::Sensitive differential scanning calorimetry was employed to investigate thylakoid membrane structure. Calorimetric scans of chloroplast membranes suspended in a low ionic strength Hepesbuffered medium revealed endothermic transitions centered at the following temperatures ( degrees C): A (42.5), B (60.6), C(1) (64.9),...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Smith KA,Low PS

    更新日期:1989-06-01 00:00:00

  • Effects of Hydroxamic Acids Isolated from Gramineae on Adenosine 5'-triphosphate Synthesis in Chloroplasts.

    abstract::Two hydroxamic acids isolated from maize extracts, 2,4-dihydroxy-7-methoxy-1,4-(2H)-benzoxazin-3(4H)-one (DIMBOA) and the 2-O-beta-d-glucopyranoside of DIMBOA, inhibit photophosphorylation by spinach chloroplasts. Both cyclic and noncyclic photophosphorylations were inhibited to the same extent. The concentrations pro...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Queirolo CB,Andreo CS,Vallejos RH,Niemeyer HM,Corcuera LJ

    更新日期:1981-10-01 00:00:00

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    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Bouquin T,Meier C,Foster R,Nielsen ME,Mundy J

    更新日期:2001-10-01 00:00:00

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    pub_type: 杂志文章


    authors: Rikin A,Schwartzbach SD

    更新日期:1989-05-01 00:00:00

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    abstract::In response to a moisture gradient, roots exhibit hydrotropism to control the orientation of their growth. To exhibit hydrotropism, however, they must overcome the gravitropism that is dominant on Earth. We found that moisture gradient or water stress caused immediate degradation of the starch anchors, amyloplasts, in...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Takahashi N,Yamazaki Y,Kobayashi A,Higashitani A,Takahashi H

    更新日期:2003-06-01 00:00:00

  • DELLA1-Mediated Gibberellin Signaling Regulates Cytokinin-Dependent Symbiotic Nodulation.

    abstract::In legume plants, low-nitrogen soils promote symbiotic interactions with rhizobial bacteria, leading to the formation of nitrogen-fixing root nodules. Among critical signals regulating this developmental process are bacterial Nod Factors (NFs) and several plant hormones, including cytokinins (CKs) and gibberellins (GA...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Fonouni-Farde C,Kisiala A,Brault M,Emery RJN,Diet A,Frugier F

    更新日期:2017-12-01 00:00:00

  • A transcriptome-based characterization of habituation in plant tissue culture.

    abstract::For the last 50 years, scientists have recognized that varying ratios of the plant hormones cytokinin and auxin induce plant cells to form particular tissues: undifferentiated calli, shoot structures, root structures, or a whole plant. Proliferation of undifferentiated callus tissue, greening, and the formation of sho...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Pischke MS,Huttlin EL,Hegeman AD,Sussman MR

    更新日期:2006-04-01 00:00:00

  • The arabidopsis cell plate-associated dynamin-like protein, ADL1Ap, is required for multiple stages of plant growth and development.

    abstract::Dynamin and dynamin-like proteins are GTP-binding proteins involved in vesicle trafficking. In soybean, a 68-kD dynamin-like protein called phragmoplastin has been shown to be associated with the cell plate in dividing cells (Gu and Verma, 1996). Five ADL1 genes encoding dynamin-like proteins related to phragmoplastin...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Kang BH,Busse JS,Dickey C,Rancour DM,Bednarek SY

    更新日期:2001-05-01 00:00:00

  • mEosFP-based green-to-red photoconvertible subcellular probes for plants.

    abstract::Photoconvertible fluorescent proteins (FPs) are recent additions to the biologists' toolbox for understanding the living cell. Like green fluorescent protein (GFP), monomeric EosFP is bright green in color but is efficiently photoconverted into a red fluorescent form using a mild violet-blue excitation. Here, we repor...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Mathur J,Radhamony R,Sinclair AM,Donoso A,Dunn N,Roach E,Radford D,Mohaghegh PS,Logan DC,Kokolic K,Mathur N

    更新日期:2010-12-01 00:00:00

  • Cytochemical localization of catalase activity in glyoxysomes from castor bean endosperm.

    abstract::Glyoxysomes isolated from castor bean (Ricinus communis L. var. zanzibariensis) endosperm have been stained by the cytochemical diaminobenzidine reaction. The reaction product obtained by preincubation with 3,3'-diaminobenzidine and incubation with the reagent and H(2)O(2) is distributed uniformly throughout the matri...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Bieglmayer C,Ruis H

    更新日期:1974-02-01 00:00:00

  • Purification and characterization of the reconstitutively active citrate carrier from maize mitochondria.

    abstract::The citrate carrier from maize (Zea mays) shoot mitochondria was solubilized with Triton X-100 and purified by sequential chromatography on hydroxyapatite and hydroxyapatite/celite in the presence of cardiolipin. SDS-gel electrophoresis of the purified fraction showed a single polypeptide band with an apparent molecul...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Genchi G,Spagnoletta A,De Santis A,Stefanizzi L,Palmieri F

    更新日期:1999-07-01 00:00:00

  • Photorespiration in c(3) and c(4) plant tissue cultures: significance of kranz anatomy to low photorespiration in c(4) plants.

    abstract::Photorespiration rates in tissue cultures of a C(4) plant, Portulaca oleracea, were compared to those in tissue cultures of a C(3) plant, Streptanthus tortuosus. The C(4) plant tissue cultures have one-half to one-third the photorespiration rate of the C(3) plant tissue cultures and respond to varying O(2) concentrati...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Kennedy RA

    更新日期:1976-10-01 00:00:00

  • Cytokinins extracted from pinto bean fruit.

    abstract::Extracts from various parts of Phaseolus vulgaris L. var. Pinto plants were found to exhibit cytokinin activity with the highest levels present in extracts of the fruit tissue. To separate the cytokinins present in the fruit, extracts were chromatographed in several solvent systems. Bioassays of chromatograms indicate...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Krasnuk M,Witham FH,Tegley JR

    更新日期:1971-09-01 00:00:00

  • Influence of chloroplast development on the activation of the diphenyl ether herbicide acifluorfen-methyl.

    abstract::The activity of acifluorfen-methyl (AFM); methyl 5-(2-chloro-4-[trifluoromethyl] phenoxy)-2-nitrobenzoate in excised cucumber cotyledons (Cucumis sativus L.) was examined. AFM induced membrane disruption, was significantly greater when etiolated cotyledons were illuminated 16 hours at 150 microeinsteins per square met...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Halling BP,Peters GR

    更新日期:1987-08-01 00:00:00

  • A Nuclear Magnetic Resonance Study of Water in Cold-acclimating Cereals.

    abstract::Continuous wave nuclear magnetic resonance (NMR) studies indicated that the line width of the water absorption peak (Deltav(1/2)) from crowns of winter and spring wheat (Triticum aestivum L.) increased during cold acclimation. There was a negative correlation between Deltav(1/2) and crown water content, and both of th...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Gusta LV,Fowler DB,Chen P

    更新日期:1979-04-01 00:00:00

  • Global selection on sucrose synthase haplotypes during a century of wheat breeding.

    abstract::Spike number per unit area, number of grains per spike, and thousand kernel weight (TKW) are important yield components. In China, increases in wheat (Triticum aestivum) yields are mainly due to increases in grain number per spike and TKW. TKW mainly depends on starch content, as starch accounts for about 70% of the g...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Hou J,Jiang Q,Hao C,Wang Y,Zhang H,Zhang X

    更新日期:2014-04-01 00:00:00

  • Steps linking the photosynthetic light reactions to the biological clock require calcium.

    abstract::The effect of modifying calcium concentration on the expression of the photosynthesis circadian rhythm was examined in Euglena gracilis, Klebs strain Z. Expression of the oxygen evolution rhythm required the presence of both extracellular and intracellular calcium. Several treatments were found to uncouple the rate of...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Lonergan TA

    更新日期:1990-05-01 00:00:00

  • Plant triose phosphate isomerase isozymes : purification, immunological and structural characterization, and partial amino Acid sequences.

    abstract::We report the first complete purifications of the cytosolic and plastid isozymes of triose phosphate isomerase (TPI; EC from higher plants including spinach (Spinacia oleracea), lettuce (Lactuca sativa), and celery (Apium graveolens). Both isozymes are composed of two isosubunits with approximate molecular we...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Pichersky E,Gottlieb LD

    更新日期:1984-02-01 00:00:00

  • Changes in Mitochondrial Respiratory Chain Components of Petunia Cells during Culture in the Presence of Antimycin A.

    abstract::When petunia (Petunia hybrida Vilm, cv Rosy Morn) cells are cultured in the presence of 2 [mu]M antimycin A (AA), respiration proceeds mainly via the cyanide-resistant pathway. Cyanide-resistant respiratory rates were higher in mitochondria from AA cells than in control mitochondria. Compared with control cells, an in...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Wagner AM,Wagner MJ

    更新日期:1997-10-01 00:00:00