Abstract:
:Germination of lettuce (Lactuca sativa) 'Grand Rapids' seeds is regulated by phytochrome. The action of phytochrome includes alterations in the levels of gibberellin (GA) and abscisic acid (ABA). To determine the molecular mechanism of phytochrome regulation of ABA metabolism, we isolated four lettuce cDNAs encoding 9-cis-epoxycarotenoid dioxygenase (biosynthesis; LsNCED1-LsNCED4) and four cDNAs for ABA 8'-hydroxylase (catabolism; LsABA8ox1-LsABA8ox4). Measurements of ABA and its catabolites showed that a decrease in ABA level coincided with a slight increase in the level of the ABA catabolite phaseic acid after red light treatment. Quantitative reverse transcription-polymerase chain reaction analysis indicated that ABA levels are controlled by phytochrome through down-regulation of LsNCED2 and LsNCED4 expression and up-regulation of LsABA8ox4 expression in lettuce seeds. Furthermore, the expression levels of LsNCED4 decreased after GA(1) treatment, whereas the levels of expression of the other two genes were unaffected. The LsNCED4 expression was also down-regulated by red light in lettuce seeds in which GA biosynthesis was suppressed by AMO-1618, a specific GA biosynthesis inhibitor. These results indicate that phytochrome regulation of ABA metabolism is mediated by both GA-dependent and -independent mechanisms. Spatial analysis showed that after red light treatment, the ABA decrease on the hypocotyl side was greater than that on the cotyledon side of lettuce seeds. Moreover, phytochrome-regulated expression of ABA and GA biosynthesis genes was observed on the hypocotyl side, rather than the cotyledon side, suggesting that this regulation occurs near the photoperceptive site.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Sawada Y,Aoki M,Nakaminami K,Mitsuhashi W,Tatematsu K,Kushiro T,Koshiba T,Kamiya Y,Inoue Y,Nambara E,Toyomasu Tdoi
10.1104/pp.107.115162subject
Has Abstractpub_date
2008-03-01 00:00:00pages
1386-96issue
3eissn
0032-0889issn
1532-2548pii
pp.107.115162journal_volume
146pub_type
杂志文章相关文献
PLANT PHYSIOLOGY文献大全abstract::The ability of 21 C3 and C4 monocot and dicot species to rapidly export newly fixed C in the light at both ambient and enriched CO2 levels was compared. Photosynthesis and concurrent export rates were estimated during isotopic equilibrium of the transport sugars using a steady-state 14CO2-labeling procedure. At ambien...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.117.1.207
更新日期:1998-05-01 00:00:00
abstract::Oxygen deprivation induces a wide variety of genes, but the most extensively studied are those encoding enzymes of the glycolytic pathway. Lactate dehydrogenase (LDH, EC 1.1.1.27) activity increases up to 3.5-fold in maize (Zea mays L.) roots during several days of hypoxic induction. This increase in activity is accom...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.112.3.1015
更新日期:1996-11-01 00:00:00
abstract::Maximizing light capture by light-harvesting pigment optimization represents an attractive but challenging strategy to improve photosynthetic efficiency. Here, we report that loss of a previously uncharacterized gene, HIGH PHOTOSYNTHETIC EFFICIENCY1 (HPE1), optimizes light-harvesting pigments, leading to improved phot...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.16.00698
更新日期:2016-11-01 00:00:00
abstract::During adaptation of barley (Hordeum vulgare L.) seedlings to extremely high concentrations of sodium chloride in the root space, the content of galactolipids of chloroplast membranes decreased considerably. Alterations in membrane lipids were due to the high concentration of ions rather than to the increase in the wa...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.62.3.326
更新日期:1978-09-01 00:00:00
abstract::E2F transcription factors are required for the progression and arrest of the cell cycle in animals. Like animals, plants have evolved to conserve the E2F family. The Arabidopsis genome encodes E2F and DP proteins that share a high similarity with the animal E2F and DP families. Here, we show that Arabidopsis E2F and D...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.010642
更新日期:2002-03-01 00:00:00
abstract::Boron (B) polyol complexes have been isolated and characterized from the phloem sap of celery (Apium graveolens L.) and the extrafloral nectar of peach (Prunus persica L.). In celery the direct analysis of untreated phloem sap by matrix-assisted laser desorption-Fourier transform mass spectrometry, with verification b...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.113.2.649
更新日期:1997-02-01 00:00:00
abstract::NAD-dependent glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a ubiquitous enzyme involved in the glycolytic pathway. It has been widely demonstrated that mammalian GAPDH, in addition to its role in glycolysis, fulfills alternative functions mainly linked to its susceptibility to oxidative posttranslational modifi...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.113.215194
更新日期:2013-05-01 00:00:00
abstract::Previous experiments using in situ RNA hybridization have shown that the mRNAs encoding phenylalanine ammonia-lyase, 4-coumarate:coenzyme A ligase, and pathogenesis-related protein 1 accumulated transiently around fungal infection sites in parsley (Petroselinum crispum) leaf buds. These studies have now been extended ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.112.1.131
更新日期:1996-09-01 00:00:00
abstract::Doubling the concentration of atmospheric CO2 often inhibits plant respiration, but the mechanistic basis of this effect is unknown. We investigated the direct effects of increasing the concentration of CO2 by 360 [mu]L L-1 above ambient on O2 uptake in isolated mitochondria from soybean (Glycine max L. cv Ransom) cot...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.112.3.1349
更新日期:1996-11-01 00:00:00
abstract::The effect of salicylic acid (SA) on respiration and mitochondrial function was examined in tobacco (Nicotiana tabacum) suspension cell cultures in the range of 0.01 to 5 mm. Cells rapidly accumulated SA up to 10-fold of the externally applied concentrations. At the lower concentrations, SA accumulation was transitory...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.103.031039
更新日期:2004-01-01 00:00:00
abstract::Hydrogen peroxide (H2O2) is a stable component of reactive oxygen species, and its production in plants represents the successful recognition of pathogen infection and pathogen-associated molecular patterns (PAMPs). This production of H2O2 is typically apoplastic but is subsequently associated with intracellular immun...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.15.01237
更新日期:2016-07-01 00:00:00
abstract::Ion and oxygen uptake were studied on aging bean stem slices. Oxygen uptake was high immediately after slicing, decreased to a minimum at 100 minutes, and then increased again. Ion uptake per unit of O(2) uptake data suggested that metabolic energy was utilized almost exclusively for sodium transport in fresh tissue b...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.47.5.663
更新日期:1971-05-01 00:00:00
abstract::Sucrose synthase (EC 2.4.1.13) of sugarbeet (Beta vulgaris L.) fibrous roots has a distinctly different order of preference for nucleoside diphosphate substrates than that of the taproot enzyme (Silvius, Snyder 1979 Plant Physiol 64: 1070-1073). Separation and quantitation of UDP, ADP, and GDP in root extracts by high...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.70.1.316
更新日期:1982-07-01 00:00:00
abstract::Light-induced conversion of NAD to NADP was investigated in higher plants. Upon illumination, conversion of NAD to NADP was observed in intact leaves of wheat and pea following incubation in the dark. This conversion was also observed in mesophyll protoplasts of wheat leaves when they were isolated in the dark or isol...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.68.2.324
更新日期:1981-08-01 00:00:00
abstract::Knowledge about physiological stress thresholds provides crucial information about plant performance and survival under drought. In this study, we report on the triphasic nature of the relationship between plant water potential (Ψ) at predawn and midday and describe a method that predicts Ψ at stomatal closure and tur...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.20.00500
更新日期:2020-10-01 00:00:00
abstract::Pollen mother cells (PMCs) represent a critical early stage in plant sexual reproduction in which the stage is set for male gamete formation. Understanding the global molecular genetics of this early meiotic stage has so far been limited to whole stamen or floret transcriptome studies, but since PMCs are a discrete po...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.110.161661
更新日期:2010-12-01 00:00:00
abstract::Syntheses are described for tetra and pentadeutero indole-3-acetic acid (IAA) labeled in positions 4, 5, 6, 7 or 2, 4, 5, 6, 7 of the indole moiety. Polydeuterated IAA is proposed as an internal standard for gas chromatographic-mass spectrometric analysis of IAA by selected ion monitoring. Nanogram amounts of IAA may ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.66.4.775
更新日期:1980-10-01 00:00:00
abstract::We have introduced an ice nucleation gene (inaZ) from Pseudomonas syringae pv. syringae into Nicotiana tabacum, a freezing-sensitive species, and Solanum commersonii, a freezing-tolerant species. Transformants of both species showed increased ice nucleation activity over untransformed controls. The concentration of ic...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.100.4.1730
更新日期:1992-12-01 00:00:00
abstract::Leaf tissues from Xerosicyos danguyi H. Humb., a succulent member of the Cucurbitaceae, were found to possess both galactinol synthase activity and the capacity for photosynthetic production of stachyose, the phloem transport oligosaccharide common to other nonsucculent cucurbits. The amounts of stachyose isolated fro...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.87.3.588
更新日期:1988-07-01 00:00:00
abstract::Suspension cultures of carrot (Daucus carota, line C1), tobacco (Nicotiana tabacum, line TX1), and Nicotiana plumbaginifolia (line NP) were frozen under controlled conditions with trehalose as the sole cryoprotectant. Maximal post-thaw viability (71-74%), measured by phenosafranin dye exclusion, was obtained with the ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.78.2.430
更新日期:1985-06-01 00:00:00
abstract::When petunia (Petunia hybrida Vilm, cv Rosy Morn) cells are cultured in the presence of 2 [mu]M antimycin A (AA), respiration proceeds mainly via the cyanide-resistant pathway. Cyanide-resistant respiratory rates were higher in mitochondria from AA cells than in control mitochondria. Compared with control cells, an in...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.115.2.617
更新日期:1997-10-01 00:00:00
abstract::We recently identified a cDNA encoding a putative isovaleryl-coenzyme A (CoA) dehydrogenase in Arabidopsis (AtIVD). In animals, this homotetrameric enzyme is located in mitochondria and catalyzes the conversion of isovaleryl-CoA to 3-methylcrotonyl-CoA as an intermediate step in the leucine (Leu) catabolic pathway. Ex...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.126.2.601
更新日期:2001-06-01 00:00:00
abstract::Steady state rates of NADP reduction decline upon commencement of nitrite reduction in reconstituted chloroplast preparations. Similarly, steady state rates of nitrite reduction are lower, but not zero, during concurrent NADP reduction. These results imply that competition for substrate occurs and suggest that nitrite...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.77.2.318
更新日期:1985-02-01 00:00:00
abstract::Stomatal movements depend on an osmoregulation process in which swelling or shrinking of the guard cells opens or closes the stomatal pore. Ions and water fluxes are an essential aspect of guard cell osmoregulation. Thus far, studies of these fluxes have focused on the guard cell plasma membrane. Guard cells, however,...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.105.3.999
更新日期:1994-07-01 00:00:00
abstract::Embryos from rice (Oryza sativa L. var. Bluebonnet) and wheat (Triticum aestivum L.) contain an aminoacyl-tRNA protein transferase which transfers arginine from arginyl-tRNA to the N terminus of a protein acceptor. The activity was measured in vitro in a reaction mixture containing embryo supernatant fraction, buffer,...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.52.1.13
更新日期:1973-07-01 00:00:00
abstract::Maize plants (Zea mays) attacked by caterpillars release a mixture of odorous compounds that attract parasitic wasps, natural enemies of the herbivores. We assessed the genetic variability of these induced volatile emissions among 31 maize inbred lines representing a broad range of genetic diversity used by breeders i...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.104.039891
更新日期:2004-08-01 00:00:00
abstract::Mildly disrupted cells of Chlamydomonas reinhardi Dangeard were incubated in a reaction medium containing glycolate, ferricyanide, and cupric ions, and then processed for electron microscopy. As a result of the cytochemical treatment, an electron opaque product was deposited specifically in the outer compartment of mi...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.58.3.315
更新日期:1976-09-01 00:00:00
abstract::A water-soluble, ethanol-insoluble extract of autolytically inactive tomato (Lycopersicon esculentum Mill.) pericarp tissue contains a series of galacturonic acid-containing (pectic) oligosaccharides that will elicit a transient increase in ethylene biosynthesis when applied to pericarp discs cut from mature green fru...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.106.2.575
更新日期:1994-10-01 00:00:00
abstract::Full-length cDNA corresponding to Arabidopsis (Arabidopsis thaliana) gene At2g31690, which has been annotated in GenBank as a putative triacylglycerol (TAG) lipase, was obtained by reverse transcription-polymerase chain reaction using RNA from senescing rosette leaves of Arabidopsis as a template. The cognate protein ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.106.090811
更新日期:2007-03-01 00:00:00
abstract::Concanavalin A, soybean (Glycine max L.) lectin, castor bean (Ricinus communis L.) lectin, and peanut (Arachis hypogaea L.) lectin were able to agglutinate protoplasts prepared from a variety of plant species. The seven other lectins tried were unable to agglutinate those protoplasts tested. Protoplasts prepared from ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.61.4.626
更新日期:1978-04-01 00:00:00