The mitochondrial isovaleryl-coenzyme a dehydrogenase of arabidopsis oxidizes intermediates of leucine and valine catabolism.

Abstract:

:We recently identified a cDNA encoding a putative isovaleryl-coenzyme A (CoA) dehydrogenase in Arabidopsis (AtIVD). In animals, this homotetrameric enzyme is located in mitochondria and catalyzes the conversion of isovaleryl-CoA to 3-methylcrotonyl-CoA as an intermediate step in the leucine (Leu) catabolic pathway. Expression of AtIVD:smGFP4 fusion proteins in tobacco (Nicotiana tabacum) protoplasts and biochemical studies now demonstrate the in vivo import of the plant isovaleryl-CoA dehydrogenase (IVD) into mitochondria and the enzyme in the matrix of these organelles. Two-dimensional separation of mitochondrial proteins by blue native and SDS-PAGE and size determination of the native and overexpressed proteins suggest homodimers to be the dominant form of the plant IVD. Northern-blot hybridization and studies in transgenic Arabidopsis plants expressing Ativd promoter:gus constructs reveal strong expression of this gene in seedlings and young plants grown in the absence of sucrose, whereas promoter activity in almost all tissues is strongly inhibited by exogeneously added sucrose. Substrate specificity tests with AtIVD expressed in Escherichia coli indicate a strong preference toward isovaleryl-CoA but surprisingly also show considerable activity with isobutyryl-CoA. This strongly indicates a commitment of the enzyme in Leu catabolism, but the activity observed with isobutyryl-CoA also suggests a parallel involvement of the enzyme in the dehydrogenation of intermediates of the valine degradation pathway. Such a dual activity has not been observed with the animal IVD and may suggest a novel connection of the Leu and valine catabolism in plants.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Däschner K,Couée I,Binder S

doi

10.1104/pp.126.2.601

subject

Has Abstract

pub_date

2001-06-01 00:00:00

pages

601-12

issue

2

eissn

0032-0889

issn

1532-2548

journal_volume

126

pub_type

杂志文章
  • Developmental Loss of Photosystem II Activity and Structure in a Chloroplast-Encoded Tobacco Mutant, Lutescens-1.

    abstract::Lutescens-1, a tobacco mutant with a maternally inherited dysfunction, displayed an unusual developmental phenotype. In vivo measurement of chlorophyll fluorescence revealed deterioration in photosystem II (PSII) function as leaves expanded. Analysis of thylakoid membrane proteins by polyacrylamide gel electrophoresis...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.82.1.19

    authors: Chia CP,Duesing JH,Arntzen CJ

    更新日期:1986-09-01 00:00:00

  • Serine Hydroxymethyltransferase from Mung Bean (Vigna radiata) Is Not a Pyridoxal-5'-Phosphate-Dependent Enzyme.

    abstract::Serine hydroxymethyltransferase from mammalian and bacterial sources is a pyridoxal-5'-phosphate-containing enzyme, but the requirement of pyridoxal-5'-phosphate for the activity of the enzyme from plant sources is not clear. The specific activity of serine hydroxymethyltransferase isolated from mung bean (Vigna radia...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.95.2.351

    authors: Sukanya N,Vijaya M,Savithri HS,Radhakrishnan AN,Rao NA

    更新日期:1991-02-01 00:00:00

  • Stimulation of root elongation and curvature by calcium.

    abstract::Ca2+ has been proposed to mediate inhibition of root elongation. However, exogenous Ca2+ at 10 or 20 millimolar, applied directly to the root cap, significantly stimulated root elongation in pea (Pisum sativum L.) and corn (Zea mays L.) seedlings. Furthermore, Ca2+ at 1 to 20 millimolar, applied unilaterally to the ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.98.1.246

    authors: Takahashi H,Scott TK,Suge H

    更新日期:1992-01-01 00:00:00

  • Purification and characterization of the reconstitutively active citrate carrier from maize mitochondria.

    abstract::The citrate carrier from maize (Zea mays) shoot mitochondria was solubilized with Triton X-100 and purified by sequential chromatography on hydroxyapatite and hydroxyapatite/celite in the presence of cardiolipin. SDS-gel electrophoresis of the purified fraction showed a single polypeptide band with an apparent molecul...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.120.3.841

    authors: Genchi G,Spagnoletta A,De Santis A,Stefanizzi L,Palmieri F

    更新日期:1999-07-01 00:00:00

  • Chorismate Mutase Isoenzymes from Selected Plants and Their Immunological Comparison with the Isoenzymes from Sorghum bicolor.

    abstract::The isoenzyme pattern of chorismate mutase (EC 5.4.99.5) was examined by diethylaminoethyl-cellulose chromatography in a wide variety of plants. All plants contained a regulated form of chorismate mutase (CM-1), and most contained an additional, unregulated form (CM-2). The regulatory properties of CM-1 differed signi...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.81.3.717

    authors: Singh BK,Lonergan SG,Conn EE

    更新日期:1986-07-01 00:00:00

  • Pollen Tube Discharge Completes the Process of Synergid Degeneration That Is Initiated by Pollen Tube-Synergid Interaction in Arabidopsis.

    abstract::In flowering plant reproduction, pollen tube reception is the signaling system that results in pollen tube discharge, synergid degeneration, and successful delivery of male gametes (two sperm cells) to the site where they can fuse with female gametes (egg cell and central cell). Some molecules required for this comple...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.15.00528

    authors: Leydon AR,Tsukamoto T,Dunatunga D,Qin Y,Johnson MA,Palanivelu R

    更新日期:2015-09-01 00:00:00

  • Reduction of Nitrate and Nitrite in Lambsquarters (Chenopodium album) Biotypes Resistant and Susceptible to Atrazine Toxicity.

    abstract::The nitrite-reducing activity of the normal susceptible biotype of lambsquarters (Chenopodium album L.) was strongly inhibited by atrazine in the assay medium, both in the case of the in vivo assays of leaf discs in light, and in vitro photoreduction assays of crude extracts. In vitro assays of crude extracts with met...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.65.5.984

    authors: Lawrence JM,Foster RJ,Herrick HE

    更新日期:1980-05-01 00:00:00

  • Effects of univalent cations on the activity of particulate starch synthetase.

    abstract::An investigation was made to determine the univalent cation requirements of starch synthetase from a variety of plant species of economic importance. The particulate enzyme from sweet corn was shown to have an absolute requirement for potassium, with the optimum activation occurring at 0.05 M KCl. Rubidium, cesium, an...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.44.9.1260

    authors: Nitsos RE,Evans HJ

    更新日期:1969-09-01 00:00:00

  • Phosphorylation of MAP65-1 by Arabidopsis Aurora Kinases Is Required for Efficient Cell Cycle Progression.

    abstract::Aurora kinases are key effectors of mitosis. Plant Auroras are functionally divided into two clades. The alpha Auroras (Aurora1 and Aurora2) associate with the spindle and the cell plate and are implicated in controlling formative divisions throughout plant development. The beta Aurora (Aurora3) localizes to centromer...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.16.01602

    authors: Boruc J,Weimer AK,Stoppin-Mellet V,Mylle E,Kosetsu K,Cedeño C,Jaquinod M,Njo M,De Milde L,Tompa P,Gonzalez N,Inzé D,Beeckman T,Vantard M,Van Damme D

    更新日期:2017-01-01 00:00:00

  • The procambium specification gene Oshox1 promotes polar auxin transport capacity and reduces its sensitivity toward inhibition.

    abstract::The auxin-inducible homeobox gene Oshox1 of rice (Oryza sativa) is a positive regulator of procambial cell fate commitment, and its overexpression reduces the sensitivity of polar auxin transport (PAT) to the PAT inhibitor 1-N-naphthylphthalamic acid (NPA). Here, we show that wild-type rice leaves formed under conditi...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.009167

    authors: Scarpella E,Boot KJ,Rueb S,Meijer AH

    更新日期:2002-11-01 00:00:00

  • Cytokinin structure-activity relationships and the metabolism of N-(delta-isopentenyl)adenosine-8-C in phaseolus callus tissues.

    abstract::The activities of the free base and ribonucleoside forms of cytokinins bearing saturated and unsaturated N(6)-isoprenoid side chains have been examined in callus cultures derived from Phaseolus vulgaris cv. Great Northern, P. lunatus cv. Kingston, and the interspecific hybrid Great Northern x Kingston. In callus of cv...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.70.1.173

    authors: Mok MC,Mok DW,Dixon SC,Armstrong DJ,Shaw G

    更新日期:1982-07-01 00:00:00

  • Isolation and Freezing Tolerances of Mesophyll Cells from Cold-hardened and Nonhardened Winter Rye.

    abstract::A method is described for the release of large quantities of mesophyll cells from leaves of both cold-hardened and nonhardened winter rye seedlings by a combination of enzymic and physical maceration. Such preparations usually contain a certain percentage of nonviable cells and are thus not suitable for quantitative b...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.67.5.906

    authors: Singh J

    更新日期:1981-05-01 00:00:00

  • Ploidy effects on anatomy and gas exchange of tall fescue leaves.

    abstract::A growth chamber study was designed to interpret differences in CO(2) exchange rate (CER) and leaf diffusive resistance among 4X, 6X, 8X, and 10X ploidy levels of tall fescue (Festuca arundinacea, Schreb). Mesophyll cell size, stomatal density, number of major and minor veins, and bundle cap size of leaf blades were e...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.68.4.891

    authors: Byrne MC,Nelson CJ,Randall DD

    更新日期:1981-10-01 00:00:00

  • Development of two isogenic sweet corn hybrids differing for glycinebetaine content.

    abstract::A hybrid of sweet corn, Zea mays L. (;1720'; Rogers Brothers Seed Co.), was found to be comprised of glycinebetaine-positive and glycinebetaine-deficient individuals in a 1:1 mixture. This phenomenon was traced to segregation for a single, nuclear, dominant gene determining leaf glycinebetaine content within the femal...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.91.3.1112

    authors: Rhodes D,Rich PJ,Brunk DG,Ju GC,Rhodes JC,Pauly MH,Hansen LA

    更新日期:1989-11-01 00:00:00

  • Variable Effects of C-Terminal Fusions on FLS2 Function: Not All Epitope Tags Are Created Equal.

    abstract::Receptor-like kinases (RLKs) are the largest family of proteins in plants and are responsible for perceiving the vast majority of extracellular stimuli. Thus, RLKs function in diverse processes, including sensing pathogen attacks, regulating symbiotic interactions, transducing hormone and peptide signals, and monitori...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.17.01700

    authors: Hurst CH,Turnbull D,Myles SM,Leslie K,Keinath NF,Hemsley PA

    更新日期:2018-06-01 00:00:00

  • Immunocytolocalization of polygalacturonase in ripening tomato fruit.

    abstract::Using tissue blotting and immunocytolocalization, we have investigated the appearance and accumulation of polygalacturonase (PG) during tomato (Lycopersicon esculentum Mill.) fruit ripening. Results show that PG first appears in the collumella region followed by sequential appearance in the exopericarp and endopericar...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.90.1.17

    authors: Tieman DM,Handa AK

    更新日期:1989-05-01 00:00:00

  • The DEAD-box RNA Helicase RH50 Is a 23S-4.5S rRNA Maturation Factor that Functionally Overlaps with the Plastid Signaling Factor GUN1.

    abstract::DEAD-box RNA helicases (DBRHs) modulate RNA secondary structure, allowing RNA molecules to adopt the conformations required for interaction with their target proteins. RH50 is a chloroplast-located DBRH that colocalizes and is coexpressed with GUN1, a central factor in chloroplast-to-nucleus signaling. When combined w...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.17.01545

    authors: Paieri F,Tadini L,Manavski N,Kleine T,Ferrari R,Morandini P,Pesaresi P,Meurer J,Leister D

    更新日期:2018-01-01 00:00:00

  • Identical amino acid substitutions in the repression domain of auxin/indole-3-acetic acid proteins have contrasting effects on auxin signaling.

    abstract::Auxin/indole-3-acetic acid (Aux/IAA) proteins function as repressors of auxin response gene expression when auxin concentrations in a cell are low. At elevated auxin concentrations, these repressors are destroyed via the ubiquitin-proteasome pathway, resulting in derepression/activation of auxin response genes. Most A...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.110.171322

    authors: Li H,Tiwari SB,Hagen G,Guilfoyle TJ

    更新日期:2011-03-01 00:00:00

  • Regulation of ribulose diphosphate formation in vivo by light.

    abstract::Light-dependent formation of ribulose-1,5 diphosphate is completely inhibited by low concentrations of 3-(3,4-dichlorophenyl)-1,1-dimethylurea which do not severely affect cyclic photophosphorylation. Also in Scenedesmus mutant number 11, capable of cyclic photophosphorylation, cellular ribulose-1,5 diphosphate-levels...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.49.6.904

    authors: Klob W,Kandler O,Tanner W

    更新日期:1972-06-01 00:00:00

  • Light Energy Dissipation under Water Stress Conditions: Contribution of Reassimilation and Evidence for Additional Processes.

    abstract::Using (14)CO(2) gas exchange and metabolite analyses, stomatal as well as total internal CO(2) uptake and evolution were estimated. Pulse modulated fluorescence was measured during induction and steady state of photosynthesis. Leaf water potential of Digitalis lanata EHRH. plants decreased to -2.5 megapascals after wi...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.92.4.1053

    authors: Stuhlfauth T,Scheuermann R,Fock HP

    更新日期:1990-04-01 00:00:00

  • Integrated Analysis of Engineered Carbon Limitation in a Quadruple CO2/HCO3- Uptake Mutant of Synechocystis sp. PCC 6803.

    abstract::Cyanobacteria have efficient carbon concentration mechanisms and suppress photorespiration in response to inorganic carbon (Ci) limitation. We studied intracellular Ci limitation in the slow-growing CO2/HCO3 (-)-uptake mutant ΔndhD3 (for NADH dehydrogenase subunit D3)/ndhD4 (for NADH dehydrogenase subunit D4)/cmpA (fo...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.15.01289

    authors: Orf I,Klähn S,Schwarz D,Frank M,Hess WR,Hagemann M,Kopka J

    更新日期:2015-11-01 00:00:00

  • A Noninvasive Technique for Monitoring Peroxidative and H2O2-Scavenging Activities during Interactions between Bacterial Plant Pathogens and Suspension Cells.

    abstract::Stimulation of active oxygen metabolism occurs during the early stages of interactions involving bacteria and plant cell suspensions. Although many cellular processes are known to affect active oxygen metabolism in plants, it is not known which of these factors affect active oxygen levels during plant-bacteria interac...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.108.1.353

    authors: Baker CJ,Harmon GL,Glazener JA,Orlandi EW

    更新日期:1995-05-01 00:00:00

  • Concentrations of Indole-3-acetic Acid and Its Esters in Avena and Zea.

    abstract::An isotope-dilution method has been developed for the assay of free indole-3-acetic acid and ester indole-3-acetic acid as measured by indole-3-acetic acid liberated by mild alkaline hydrolysis. Application of this method to seedlings of Avena sativa and Zea mays indicates the upper limit of free indole-3-acetic acid ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.54.3.257

    authors: Bandurski RS,Schulze A

    更新日期:1974-09-01 00:00:00

  • Role of asparagine in the photorespiratory nitrogen metabolism of pea leaves.

    abstract::In pea leaves, much of the metabolism of imported asparagine is by transamination. This activity was previously shown to be localized in the peroxisomes, suggesting a possible connection between asparagine and photorespiratory nitrogen metabolism. This was investigated by examination of the transfer of (15)N from the ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.78.2.334

    authors: Ta TC,Joy KW,Ireland RJ

    更新日期:1985-06-01 00:00:00

  • Collection and Identification of Allelopathic Compounds from the Undisturbed Root System of Bigalta Limpograss (Hemarthria altissima).

    abstract::Collection of allelopathic chemicals from the undisturbed plant root system is difficult because of their low concentrations and the high level of contaminants in growth media such as soil. A new approach for the continuous trapping of quantities of extracellular chemicals from donor plants is described. Bigalta limpo...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.69.1.155

    authors: Tang CS,Young CC

    更新日期:1982-01-01 00:00:00

  • Differential transcription of phycobiliprotein components in Rhodella violacea. Light and nitrogen effects on the 33-kilodalton phycoerythrin rod linker polypeptide, phycocyanin, and phycoerythrin transcripts.

    abstract::In Rhodella violacea phycoerythrin (PE) has two transcripts, a premessenger and a mature messenger (the gene contains an intron). Phycocyanin, which is plastid-encoded, and the 33-kD PE rod linker polypeptide, which is nuclear-encoded, have only one transcript. The PE premessenger had a rapid turnover; mature transcri...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.112.3.1045

    authors: Lichtlé C,Garnier F,Bernard C,Zabulon G,Spilar A,Thomas JC,Etienne AL

    更新日期:1996-11-01 00:00:00

  • Effect of temperature on photosynthesis and growth in marine Synechococcus spp.

    abstract::In this study, we develop a mechanistic understanding of how temperature affects growth and photosynthesis in 10 geographically and physiologically diverse strains of Synechococcus spp. We found that Synechococcus spp. are able to regulate photochemistry over a range of temperatures by using state transitions and alte...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.113.221937

    authors: Mackey KR,Paytan A,Caldeira K,Grossman AR,Moran D,McIlvin M,Saito MA

    更新日期:2013-10-01 00:00:00

  • The glossy1 locus of maize and an epidermis-specific cDNA from Kleinia odora define a class of receptor-like proteins required for the normal accumulation of cuticular waxes.

    abstract::Mutations at the glossy1 (gl1) locus of maize (Zea mays L.) quantitatively and qualitatively affect the deposition of cuticular waxes on the surface of seedling leaves. The gl1 locus has been molecularly cloned by transposon tagging with the Mutator transposon system. The epi23 cDNA was isolated by subtractive hybridi...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.113.4.1091

    authors: Hansen JD,Pyee J,Xia Y,Wen TJ,Robertson DS,Kolattukudy PE,Nikolau BJ,Schnable PS

    更新日期:1997-04-01 00:00:00

  • The PsENOD12 Gene Is Expressed at Two Different Sites in Afghanistan Pea Pseudonodules Induced by Auxin Transport Inhibitors.

    abstract::A number of early nodulin genes are expressed in specific cell types as pea (Pisum sativum) root nodules develop. The Pisum sativum early nodulin PsENOD2 is detected only in the uninfected cells of the nodule parenchyma, whereas PsENOD12 is expressed at two spatially removed sites: in root hairs and adjacent cortical ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.100.4.1649

    authors: Scheres B,McKhann HI,Zalensky A,Löbler M,Bisseling T,Hirsch AM

    更新日期:1992-12-01 00:00:00

  • Necessary conditions for isolation of tightly coupled higher plant mitochondria.

    abstract::A number of parameters associated with the isolation of plant mitochondria were carefully examined in the hope of optimizing the isolation technique. Dark-grown mung bean (Phaseolus aureus var. Jumbo) hypocotyls were used as the source of mitochondria. Necessary conditions for the isolation of tightly coupled plant mi...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.45.6.773

    authors: Ikuma H

    更新日期:1970-06-01 00:00:00