Cytochemical localization of glycolate dehydrogenase in mitochondria of chlamydomonas.

Abstract:

:Mildly disrupted cells of Chlamydomonas reinhardi Dangeard were incubated in a reaction medium containing glycolate, ferricyanide, and cupric ions, and then processed for electron microscopy. As a result of the cytochemical treatment, an electron opaque product was deposited specifically in the outer compartment of mitochondria; other cellular components, including microbodies, did not accumulate stain. Incubation with d-lactate yielded similar results, while treatment with l-lactate produced only a weak reaction. Oxamate, which inhibits glycolate dehydrogenase activity in cell-free extracts, also inhibited the cytochemical reaction. These findings demonstrate in situ that glycolate dehydrogenase is localized in mitochondria, and thus corroborate similar conclusions reached on the basis of enzymic studies of isolated algal organelles.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Beezley BB,Gruber PJ,Frederick SE

doi

10.1104/pp.58.3.315

subject

Has Abstract

pub_date

1976-09-01 00:00:00

pages

315-9

issue

3

eissn

0032-0889

issn

1532-2548

journal_volume

58

pub_type

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