Abstract:
:In this study, the pathway of β-citraurin biosynthesis, carotenoid contents and the expression of genes related to carotenoid metabolism were investigated in two varieties of Satsuma mandarin (Citrus unshiu), Yamashitabeni-wase, which accumulates β-citraurin predominantly, and Miyagawa-wase, which does not accumulate β-citraurin. The results suggested that CitCCD4 (for Carotenoid Cleavage Dioxygenase4) was a key gene contributing to the biosynthesis of β-citraurin. In the flavedo of Yamashitabeni-wase, the expression of CitCCD4 increased rapidly from September, which was consistent with the accumulation of β-citraurin. In the flavedo of Miyagawa-wase, the expression of CitCCD4 remained at an extremely low level during the ripening process, which was consistent with the absence of β-citraurin. Functional analysis showed that the CitCCD4 enzyme exhibited substrate specificity. It cleaved β-cryptoxanthin and zeaxanthin at the 7,8 or 7',8' position. But other carotenoids tested in this study (lycopene, α-carotene, β-carotene, all-trans-violaxanthin, and 9-cis-violaxanthin) were not cleaved by the CitCCD4 enzyme. The cleavage of β-cryptoxanthin and zeaxanthin by CitCCD4 led to the formation of β-citraurin. Additionally, with ethylene and red light-emitting diode light treatments, the gene expression of CitCCD4 was up-regulated in the flavedo of Yamashitabeni-wase. These increases in the expression of CitCCD4 were consistent with the accumulation of β-citraurin in the two treatments. These results might provide new strategies to improve the carotenoid contents and compositions of citrus fruits.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Ma G,Zhang L,Matsuta A,Matsutani K,Yamawaki K,Yahata M,Wahyudi A,Motohashi R,Kato Mdoi
10.1104/pp.113.223297subject
Has Abstractpub_date
2013-10-01 00:00:00pages
682-95issue
2eissn
0032-0889issn
1532-2548pii
pp.113.223297journal_volume
163pub_type
杂志文章相关文献
PLANT PHYSIOLOGY文献大全abstract::Distribution of K, Ca, Cl, S, and P in freeze-dried sections of Arabidopsis flower stalk was analyzed by energy dispersive x-ray imaging. Concentrations of these elements in different cell types were quantified by microanalysis of single-cell samples and phloem exudates. Results showed a differential pattern of distri...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.124.2.599
更新日期:2000-10-01 00:00:00
abstract::Truncated transcription factor-like proteins called microProteins (miPs) can modulate transcription factor activities, thereby increasing transcriptional regulatory complexity. To understand their prevalence, evolution, and function, we predicted over 400 genes that encode putative miPs from Arabidopsis (Arabidopsis t...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.114.235903
更新日期:2014-05-01 00:00:00
abstract::An Arabidopsis (Arabidopsis thaliana) L. Heynh mutant deficient in an isoform of adenylate kinase (ADK; At2g37250) was isolated by reverse genetics. It contains a T-DNA insertion 377 bp downstream of the start point of transcription. The mutant lacks At2g37250 transcripts and has a mild reduction in total cellular ADK...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.104.056143
更新日期:2005-01-01 00:00:00
abstract::Carotenoids are essential pigments of the photosynthetic apparatus and an indispensable component of the human diet. In addition to being potent antioxidants, they also provide the vitamin A precursor beta-carotene. In tomato (Solanum lycopersicum) fruits, carotenoids accumulate in specialized plastids, the chromoplas...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.109.140533
更新日期:2009-09-01 00:00:00
abstract::Metal accumulation in seeds is a prerequisite for germination and establishment of plants but also for micronutrient delivery to humans. To investigate metal transport processes and their interactions in seeds, we focused on METAL TOLERANCE PROTEIN8 (MTP8), a tonoplast transporter of the manganese (Mn) subclade of cat...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.16.01646
更新日期:2017-07-01 00:00:00
abstract::There has been considerable interest recently in the prospect of engineering crops to produce triacylglycerol (TAG) in their vegetative tissues as a means to achieve a step change in oil yield. Here, we show that disruption of TAG hydrolysis in the Arabidopsis (Arabidopsis thaliana) lipase mutant sugar-dependent1 (sdp...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.113.219840
更新日期:2013-07-01 00:00:00
abstract::Previously, in an effort to better understand the male contribution to fertilization, we completed a maize (Zea mays) sperm expressed sequence tag project. Here, we used this resource to identify promoters that would direct gene expression in sperm cells. We used reverse transcription-polymerase chain reaction to iden...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.104.054213
更新日期:2005-08-01 00:00:00
abstract::The expression of alternative oxidase (Aox) and uncoupling proteins (Ucp) was investigated during ripening in mango (Mangifera indica) and compared with the expression of peroxisomal thiolase, a previously described ripening marker in mango. The multigene family for the Aox in mango was expressed differentially during...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.126.4.1619
更新日期:2001-08-01 00:00:00
abstract::A number of plants have been surveyed with respect to isolation by mild grinding in large quantities of leaf cells. The extent of recovery of mesophyll cells per unit leaf area was found to vary with plant species and the method of grinding. Greater than 70% recovery was obtained from the leaves of Canna indica L., Cr...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.54.4.569
更新日期:1974-10-01 00:00:00
abstract::Biochemical and electrophysiological studies on plant vacuolar transporters became feasible in the late 1970s and early 1980s, when methods to isolate large quantities of intact vacuoles and purified vacuolar membrane vesicles were established. However, with the exception of the H+-ATPase and H+-PPase, which could be ...
journal_title:Plant physiology
pub_type: 杂志文章,评审
doi:10.1104/pp.17.01481
更新日期:2018-02-01 00:00:00
abstract::Arbuscular mycorrhiza (AM) is a widespread symbiotic association between plants and fungal microsymbionts that supports plant development under nutrient-limiting and various stress conditions. In this study, we focused on the overlapping genetic program activated by two commonly studied microsymbionts in addition to i...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.104.056572
更新日期:2005-04-01 00:00:00
abstract::Constitutively produced extracellular pectinesterases from culture filtrates of the potato late blight fungus Phytophthora infestans were purified and characterized. One enzyme (PE II) was purified to homogeneity. Sodium dodecyl sulfate electrophoresis of the second enzyme (PE I) revealed two protein bands; there are ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.77.1.109
更新日期:1985-01-01 00:00:00
abstract::Using a novel screening procedure, we have selected a new class of mutant from the cyanobacterium Synechococcus PCC7942 that fails to adapt to growth at an extremely low inorganic carbon (Ci) concentration. The mutant (Tm17) reported in this study grows normally at or above air levels of CO2 (340 [mu]L L-1) but does n...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.104.2.605
更新日期:1994-02-01 00:00:00
abstract::Sections of sugar beet (Beta vulgaris L.) taproot were incubated in various concentrations of mannitol. At 0.4, 0.6, and 0.8 molar, the membrane electrical potential difference (E(m)) averaged about -130 millivolts; at 0.2 molar, about -90 millivolts; and at 0 molar, between -60 and -80 millivolts. Additions of 10 mil...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.82.4.1148
更新日期:1986-12-01 00:00:00
abstract::Lutescens-1, a tobacco mutant with a maternally inherited dysfunction, displayed an unusual developmental phenotype. In vivo measurement of chlorophyll fluorescence revealed deterioration in photosystem II (PSII) function as leaves expanded. Analysis of thylakoid membrane proteins by polyacrylamide gel electrophoresis...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.82.1.19
更新日期:1986-09-01 00:00:00
abstract::A large proton leak not coupled to ATP synthesis (slip) occurs at alkaline pH through the chloroplast ATP synthase (Y. Evron, M. Avron [1990] Biochim Biophys Acta 1019: 115-120). The involvement of the ATP synthase [gamma]-subunit in the regulation of proton conductance was analyzed by measuring the effect of thiolalk...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.115.4.1549
更新日期:1997-12-01 00:00:00
abstract::The temporal sequence of development of the major proteins of seeds of soybean (Merr.) has been studied during development of cotyledons from flowering to maturity. A well-defined difference occurred in the times of appearance and the periods of maximum accumulation of alpha, alpha'-, and beta-subunits of betaconglyci...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.67.5.958
更新日期:1981-05-01 00:00:00
abstract::Red-light irradiation of etiolated wheat (Triticum aestivum L.) leaf protoplasts rapidly increases calcium-dependent phosphorylation in vivo of 70- and 60-kD peptides, and the phosphorylation is attenuated by simultaneous far-red light (K.M. Fallon, P.S. Shacklock, A.J. Trewavas [1993] Plant Physiology 101:1039-1045)....
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.105.1.253
更新日期:1994-05-01 00:00:00
abstract::In response to a moisture gradient, roots exhibit hydrotropism to control the orientation of their growth. To exhibit hydrotropism, however, they must overcome the gravitropism that is dominant on Earth. We found that moisture gradient or water stress caused immediate degradation of the starch anchors, amyloplasts, in...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.102.018853
更新日期:2003-06-01 00:00:00
abstract::Cellular Na(+)/K(+) ratio is a crucial parameter determining plant salinity stress resistance. We tested the function of plasma membrane Na(+)/K(+) cotransporters in the High-affinity K(+) Transporter (HKT) family from the halophytic Arabidopsis (Arabidopsis thaliana) relative Thellungiella salsuginea. T. salsuginea c...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.111.193110
更新日期:2012-03-01 00:00:00
abstract::The patterns of nitrate reductase activity (NRA) in the leaves (in vivo assay) and root nodule nitrogenase activity (C(2)H(2) reduction) were investigated throughout the season in field-grown Phaseolus vulgaris plants.Maximal NRA (per g fresh weight) occurred at early stages of leaf development but total activity (per...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.63.3.421
更新日期:1979-03-01 00:00:00
abstract::Localization of mRNAs at the subcellular level is an essential mechanism for specific protein targeting and local control of protein synthesis in both eukaryotes and bacteria. While mRNA localization is well documented in metazoans, somatic cells, and microorganisms, only a handful of well-defined mRNA localization ex...
journal_title:Plant physiology
pub_type: 杂志文章,评审
doi:10.1104/pp.19.00972
更新日期:2020-01-01 00:00:00
abstract::In pea leaves, much of the metabolism of imported asparagine is by transamination. This activity was previously shown to be localized in the peroxisomes, suggesting a possible connection between asparagine and photorespiratory nitrogen metabolism. This was investigated by examination of the transfer of (15)N from the ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.78.2.334
更新日期:1985-06-01 00:00:00
abstract::ASSIMILATORY NITRATE REDUCTASE (NADH: nitrate oxidoreductase, EC 1.6.6.1) from Chlorella vulgaris has been purified to electrophoretic homogeneity with an overall yield of 60% by a procedure that utilizes blue dextran-agarose as an affinity column. Nitrate reductase binds to blue dextran and is not eluted in the prese...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.56.6.853
更新日期:1975-12-01 00:00:00
abstract::The diffusion efficiency of oxygen in the atmosphere, like that of CO2, is approximately 104 times greater than that in aqueous environments. Consequently, terrestrial photosynthetic organisms need mechanisms to protect against potential oxidative damage. The liverwort Marchantia polymorpha, a basal land plant, has ha...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.16.01038
更新日期:2017-03-01 00:00:00
abstract::Arcelin, a seed protein originally discovered in wild bean accessions, was purified, characterized, and compared to phaseolin, the major seed protein of common bean, and to phytohemagglutinin (PHA), the major bean seed lectin. Arcelin and PHA has several characteristics in common. Both were glycoproteins having simila...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.86.2.399
更新日期:1988-02-01 00:00:00
abstract::A hybrid of sweet corn, Zea mays L. (;1720'; Rogers Brothers Seed Co.), was found to be comprised of glycinebetaine-positive and glycinebetaine-deficient individuals in a 1:1 mixture. This phenomenon was traced to segregation for a single, nuclear, dominant gene determining leaf glycinebetaine content within the femal...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.91.3.1112
更新日期:1989-11-01 00:00:00
abstract::Through a biochemical and a genetic approach, we have identified several plant genes encoding methylthioribose (MTR) kinase, an enzyme involved in recycling of methionine through the methylthioadenosine (MTA) cycle. OsMTK1, an MTR kinase from rice (Oryza sativa), is 48.6 kD in size and shows cooperative kinetics with ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.104.053587
更新日期:2004-12-01 00:00:00
abstract::After incubation for 1 hr with (75)Se-selenate, excised roots of Astragalus crotalariae, a selenium-accumulating species, and A. lentiginosus, a nonaccumulator, had absorbed radioactivity to levels well over the external concentration. About 98% of the radioactivity was ethanol-soluble, and when analyzed by column and...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.44.6.893
更新日期:1969-06-01 00:00:00
abstract::Auxin induction of the proliferation of Nicotiana tabacum (cv Xanthi) mesophyll protoplasts and of protoplast-derived cells was studied. The growth-promoting properties and cytotoxicities at high concentrations of IAA and naphthaleneacetic acid were strongly affected by cell density. The induction of growth by 2,4-dic...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.75.1.54
更新日期:1984-05-01 00:00:00