Purification and Characterization of Extracellular Pectinesterases from Phytophthora infestans.

Abstract:

:Constitutively produced extracellular pectinesterases from culture filtrates of the potato late blight fungus Phytophthora infestans were purified and characterized. One enzyme (PE II) was purified to homogeneity. Sodium dodecyl sulfate electrophoresis of the second enzyme (PE I) revealed two protein bands; there are indications that both proteins are pectinesterases, which were not separable by a number of different techniques. Thus, P. infestans might produce three pectinesterases in vitro. Enzyme activities were optimal in the neutral pH range and were largely dependent on the presence of NaCl or CaCl(2) in the reaction medium. The molecular weight of the PE I-complex was between 45 and 48 kilodaltons, and the one of PE II was between 35 and 40 kilodaltons. Further investigations will help us to clarify the role of these enzymes during pathogenesis.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Förster H,Rasched I

doi

10.1104/pp.77.1.109

subject

Has Abstract

pub_date

1985-01-01 00:00:00

pages

109-12

issue

1

eissn

0032-0889

issn

1532-2548

journal_volume

77

pub_type

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