Abstract:
:Constitutively produced extracellular pectinesterases from culture filtrates of the potato late blight fungus Phytophthora infestans were purified and characterized. One enzyme (PE II) was purified to homogeneity. Sodium dodecyl sulfate electrophoresis of the second enzyme (PE I) revealed two protein bands; there are indications that both proteins are pectinesterases, which were not separable by a number of different techniques. Thus, P. infestans might produce three pectinesterases in vitro. Enzyme activities were optimal in the neutral pH range and were largely dependent on the presence of NaCl or CaCl(2) in the reaction medium. The molecular weight of the PE I-complex was between 45 and 48 kilodaltons, and the one of PE II was between 35 and 40 kilodaltons. Further investigations will help us to clarify the role of these enzymes during pathogenesis.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Förster H,Rasched Idoi
10.1104/pp.77.1.109subject
Has Abstractpub_date
1985-01-01 00:00:00pages
109-12issue
1eissn
0032-0889issn
1532-2548journal_volume
77pub_type
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