Xyloglucan galactosyl- and fucosyltransferase activities from pea epicotyl microsomes.

Abstract:

:Microsomal membranes from growing tissue of pea (Pisum sativum L.) epicotyls were incubated with the substrate UDP-[14C]galactose (Gal) with or without tamarind seed xyloglucan (XG) as a potential galactosyl acceptor. Added tamarind seed XG enhanced incorporation of [14C]Gal into high-molecular-weight products (eluted from columns of Sepharose CL-6B in the void volume) that were trichloroacetic acid-soluble but insoluble in 67% ethanol. These products were hydrolyzed by cellulase to fragments comparable in size to XG subunit oligosaccharides. XG-dependent galactosyltransferase activity could be solubilized, along with XG fucosyltransferase, by the detergent 3-[(3-cholamidopropyl)-dimethylammonio]-1-propanesulfonate. When this enzyme was incubated with tamarind (Tamarindus indica L.) seed XG or nasturtium (Tropaeolum majus L.) seed XG that had been partially degalactosylated with an XG-specific beta-galactosidase, the rates of Gal transfer increased and fucose transfer decreased compared with controls with native XG. The reaction products were hydrolyzed by cellulase to 14C fragments that were analyzed by gel-filtration and high-performance liquid chromatography fractionation with pulsed amperometric detection. The major components were XG subunits, namely one of the two possible monogalactosyl octasaccharides (-XXLG-) and digalactosyl nonasaccharide (-XLLG-), whether the predominant octasaccharide in the acceptor was XXLG (as in tamarind seed XG) or XLXG (as in nasturtium seed XG). It is concluded that the first xylosylglucose from the reducing end of the subunits was the Gal acceptor locus preferred by the solubilized pea transferase. These observations are incorporated into a model for the biosynthesis of cell wall XGs.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Faïk A,Chileshe C,Sterling J,Maclachlan G

doi

10.1104/pp.114.1.245

subject

Has Abstract

pub_date

1997-05-01 00:00:00

pages

245-54

issue

1

eissn

0032-0889

issn

1532-2548

pii

114/1/245

journal_volume

114

pub_type

杂志文章
  • B-Box Containing Proteins BBX30 and BBX31, Acting Downstream of HY5, Negatively Regulate Photomorphogenesis in Arabidopsis.

    abstract::Light-mediated seedling development is coordinately controlled by a variety of key regulators. Here, we identified two B-box (BBX)-containing proteins, BBX30 and BBX31, as repressors of photomorphogenesis. ELONGATED HYPOCOTYL5, a central regulator of light signaling, directly binds to the G-box cis-element present in ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.18.01244

    authors: Heng Y,Lin F,Jiang Y,Ding M,Yan T,Lan H,Zhou H,Zhao X,Xu D,Deng XW

    更新日期:2019-05-01 00:00:00

  • CYP704B1 is a long-chain fatty acid omega-hydroxylase essential for sporopollenin synthesis in pollen of Arabidopsis.

    abstract::Sporopollenin is the major component of the outer pollen wall (exine). Fatty acid derivatives and phenolics are thought to be its monomeric building blocks, but the precise structure, biosynthetic route, and genetics of sporopollenin are poorly understood. Based on a phenotypic mutant screen in Arabidopsis (Arabidopsi...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.109.144469

    authors: Dobritsa AA,Shrestha J,Morant M,Pinot F,Matsuno M,Swanson R,Møller BL,Preuss D

    更新日期:2009-10-01 00:00:00

  • Comparative Expression Profiling Reveals Genes Involved in Megasporogenesis.

    abstract::Megasporogenesis is a key step during ovule development in angiosperms, but the small number and inaccessibility of these cells have hampered molecular and genome-wide studies. Thus, many questions remain regarding the molecular basis of cell specification, differentiation, and development in the female gametophyte. H...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.19.01254

    authors: Zhao H,Guo M,Yan M,Cheng H,Liu Y,She Z,Lai L,Shi C,Zhang M,Li Y,Lin D,Qin Y

    更新日期:2020-04-01 00:00:00

  • Phytochrome B and at Least One Other Phytochrome Mediate the Accelerated Flowering Response of Arabidopsis thaliana L. to Low Red/Far-Red Ratio.

    abstract::We have investigated the involvement of phytochrome B in the early-flowering response of Arabidopsis thaliana L. seedlings to low red:far-red (R/FR) ratio light conditions. The phytochrome B-deficient hy3 (phyB) mutant is early flowering, and in this regard it resembles the shade-avoidance phenotype of its isogenic wi...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.104.4.1311

    authors: Halliday KJ,Koornneef M,Whitelam GC

    更新日期:1994-04-01 00:00:00

  • Association of Plant p40 Protein with Ribosomes Is Enhanced When Polyribosomes Form during Periods of Active Tissue Growth.

    abstract::p40s are acidic proteins of eukaryotic cells occurring either free in the cytoplasm or in association with ribosomes, the latter occurring in both monosomes and polysomes. p40s may play a role in the regulation of protein synthesis, although the exact mechanism is not known. Leaves of all 10 plant species examined her...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.111.2.559

    authors: Garcia-Hernandez M,Davies E,Baskin TI,Staswick PE

    更新日期:1996-06-01 00:00:00

  • Comparison between a Stable NaCl-Selected Nicotiana Cell Line and the Wild Type : K, Na, and Proline Pools as a Function of Salinity.

    abstract::An NaCl-resistant line has been developed from suspension-cultured tobacco cells (Nicotiana tabacum/gossii) by stepwise increases in the NaCl concentration in the medium. Resistance showed stability through at least 24 generations in the absence of added NaCl.Above an external NaCl concentration of 35 millimolar, prol...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.73.3.624

    authors: Watad AE,Reinhold L,Lerner HR

    更新日期:1983-11-01 00:00:00

  • Isolation and Characterization of the Multiple 7S Globulins of Soybean Proteins.

    abstract::Two major proteins (the 7S and 11S globulins) of soybean (Glycine max) were simultaneously isolated by a simple method based on their different solubilities in dilute tris (hydroxymethyl) aminomethane buffers. The purified 7S globulins, which represented essentially the entire 7S soybean protein fraction capable of di...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.56.1.19

    authors: Thanh VH,Okubo K,Shibasaki K

    更新日期:1975-07-01 00:00:00

  • Inhibition of ethylene production by cobaltous ion.

    abstract::The effect of Co(2+) on ethylene production by mung bean (Phaseolus aureus Roxb.) and by apple tissues was studied. Co(2+), depending on concentrations applied, effectively inhibited ethylene production by both tissues. It also strongly inhibited the ethylene production induced by IAA, kinetin, IAA plus kinetin, Ca(2+...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.58.1.114

    authors: Lau OL,Yang SF

    更新日期:1976-07-01 00:00:00

  • In Vivo Photomodification of Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase Holoenzyme by Ultraviolet-B Radiation (Formation of a 66-Kilodalton Variant of the Large Subunit).

    abstract::Increased levels of solar ultraviolet (290-320 nm) (UV-B) radiation could have profound effects on plant proteins because the aromatic amino acids in proteins absorb strongly in this spectral region. We have investigated the effects of UV-B radiation on plant proteins and have observed a novel 66-kD protein. This prod...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.109.1.221

    authors: Wilson MI,Ghosh S,Gerhardt KE,Holland N,Babu TS,Edelman M,Dumbroff EB,Greenberg BM

    更新日期:1995-09-01 00:00:00

  • Sugar Potentiation of Fatty Acid and Triacylglycerol Accumulation.

    abstract::Photosynthetically derived sugar provides carbon skeletons for lipid biosynthesis. We used mutants of Arabidopsis (Arabidopsis thaliana) and the expression of oleogenic factors to investigate relationships among sugar availability, lipid synthesis, and the accumulation of triacylglycerol (TAG) in leaf tissue. The adg1...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.17.00828

    authors: Zhai Z,Liu H,Xu C,Shanklin J

    更新日期:2017-10-01 00:00:00

  • Responses of Cultured Tobacco Cells to Cryptogein, a Proteinaceous Elicitor from Phytophthora cryptogea: Possible Plasmalemma Involvement.

    abstract::In culture, the phytopathogenic fungus Phytophthora cryptogea secretes a protein which elicits hypersensitive-like necroses and protects tobacco plants against invasion by the pathogen Phytophthora parasitica var. nicotianae. This protein, named cryptogein, has been purified and its amino acid sequence determined. In ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.95.2.486

    authors: Blein JP,Milat ML,Ricci P

    更新日期:1991-02-01 00:00:00

  • Effect on Root Growth of Endogenous and Applied IAA and ABA: A Critical Reexamination.

    abstract::Applications of indole-3yl-acetic acid (IAA) and abscisic acid (ABA) were done on two-day-old intact maize (cv LG 11) roots. The effect of the treatment on the root growth depends on their initial elongation rate. The slow growing roots were all inhibited by exogenous IAA and ABA at any concentrations used whereas for...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.83.1.33

    authors: Pilet PE,Saugy M

    更新日期:1987-01-01 00:00:00

  • Physiological roles of the light, oxygen, or voltage domains of phototropin 1 and phototropin 2 in Arabidopsis.

    abstract::Phototropins (phot1 and phot2) are plant blue-light receptors that mediate phototropism, chloroplast movement, stomatal opening, rapid inhibition of growth of etiolated seedlings, and leaf expansion in Arabidopsis (Arabidopsis thaliana). Their N-terminal region contains two light, oxygen, or voltage (LOV) domains, whi...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.106.089839

    authors: Cho HY,Tseng TS,Kaiserli E,Sullivan S,Christie JM,Briggs WR

    更新日期:2007-01-01 00:00:00

  • Osmotic adjustment, symplast volume, and nonstomatally mediated water stress inhibition of photosynthesis in wheat.

    abstract::At low water potential (psi(w)), dehydration reduces the symplast volume of leaf tissue. The effect of this reduction on photosynthetic capacity was investigated. The influence of osmotic adjustment on this relationship was also examined. To examine these relationships, comparative studies were undertaken on two wheat...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.85.4.1040

    authors: Gupta AS,Berkowitz GA

    更新日期:1987-12-01 00:00:00

  • The Arabidopsis embryo mutant schlepperless has a defect in the chaperonin-60alpha gene.

    abstract::We identified a T-DNA-generated mutation in the chaperonin-60alpha gene of Arabidopsis that produces a defect in embryo development. The mutation, termed schlepperless (slp), causes retardation of embryo development before the heart stage, even though embryo morphology remains normal. Beyond the heart stage, the slp m...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.126.2.717

    authors: Apuya NR,Yadegari R,Fischer RL,Harada JJ,Zimmerman JL,Goldberg RB

    更新日期:2001-06-01 00:00:00

  • The transcribed 165-bp CentO satellite is the major functional centromeric element in the wild rice species Oryza punctata.

    abstract::Centromeres are required for faithful segregation of chromosomes in cell division. It is not clear what kind of sequences act as functional centromeres and how centromere sequences are organized in Oryza punctata, a BB genome species. In this study, we found that the CentO centromeric satellites in O. punctata share h...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.105.064147

    authors: Zhang W,Yi C,Bao W,Liu B,Cui J,Yu H,Cao X,Gu M,Liu M,Cheng Z

    更新日期:2005-09-01 00:00:00

  • Glutamine Synthetase/Glutamine: alpha-Ketoglutarate Aminotransferase in Chloroplasts from the Marine Alga Caulerpa simpliciuscula.

    abstract::The enzymic capacities for ammonia assimilation into amino acids have been investigated in chloroplasts from the siphonous green alga Caulerpa simpliciuscula (Turner) C. Ag. The results show that these chloroplasts differ from those of higher plants in having present simultaneously the enzymic capacities to permit ass...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.63.3.578

    authors: McKenzie GH,Ch'ng AL,Gayler KR

    更新日期:1979-03-01 00:00:00

  • Solubilization of the starch-granule-bound starch synthase of normal maize kernels.

    abstract::The starch-granule-bound starch synthase from Zea mays has been solubilized with a recovery of between 50 and 84%. Chromatography of the solubilized enzyme on DEAE-Sepharose resolves two fractions of activity which may be distinguished by their response to citrate. Neither solubilized isoenzyme displays any significan...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.73.1.175

    authors: Macdonald FD,Preiss J

    更新日期:1983-09-01 00:00:00

  • Arabidopsis map-based cloning in the post-genome era.

    abstract::Map-based cloning is an iterative approach that identifies the underlying genetic cause of a mutant phenotype. The major strength of this approach is the ability to tap into a nearly unlimited resource of natural and induced genetic variation without prior assumptions or knowledge of specific genes. One begins with an...

    journal_title:Plant physiology

    pub_type: 杂志文章,评审

    doi:10.1104/pp.003533

    authors: Jander G,Norris SR,Rounsley SD,Bush DF,Levin IM,Last RL

    更新日期:2002-06-01 00:00:00

  • Seasonal Patterns of Nitrate Reductase and Nitrogenase Activities in Phaseolus vulgaris L.

    abstract::The patterns of nitrate reductase activity (NRA) in the leaves (in vivo assay) and root nodule nitrogenase activity (C(2)H(2) reduction) were investigated throughout the season in field-grown Phaseolus vulgaris plants.Maximal NRA (per g fresh weight) occurred at early stages of leaf development but total activity (per...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.63.3.421

    authors: Franco AA,Pereira JC,Neyra CA

    更新日期:1979-03-01 00:00:00

  • DeltapH-dependent photosystem II fluorescence quenching induced by saturating, multiturnover pulses in red algae

    abstract::We have previously shown that in the red alga Rhodella violacea, exposure to continuous low intensities of light 2 (green light) or near-saturating intensities of white light induces a DeltapH-dependent PSII fluorescence quenching. In this article we further characterize this fluorescence quenching by using white, sat...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.118.1.103

    authors: Delphin E,Duval JC,Etienne AL,Kirilovsky D

    更新日期:1998-09-01 00:00:00

  • Nod factor elicits two separable calcium responses in Medicago truncatula root hair cells.

    abstract::Modulation of intracellular calcium levels plays a key role in the transduction of many biological signals. Here, we characterize early calcium responses of wild-type and mutant Medicago truncatula plants to nodulation factors produced by the bacterial symbiont Sinorhizobium meliloti using a dual-dye ratiometric imagi...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.005546

    authors: Shaw SL,Long SR

    更新日期:2003-03-01 00:00:00

  • Photoperiodic and genetic control of carbon partitioning in peas and its relationship to apical senescence.

    abstract::Apical senescence but not flower initiation is delayed by short days (SD) compared to long days (LD) in pea plants (Pisum sativum L.) of genotype E Sn Hr. We recently reported that delay of senescence correlated with slower reproductive development, suggesting that fruits are weaker sinks for assimilates under delayed...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.86.3.978

    authors: Kelly MO,Davies PJ

    更新日期:1988-03-01 00:00:00

  • Effect of Temperature, CO(2) Concentration, and Light Intensity on Oxygen Inhibition of Photosynthesis in Wheat Leaves.

    abstract::The effect of 21% O(2) and 3% O(2) on the CO(2) exchange of detached wheat leaves was measured in a closed system with an infrared carbon dioxide analyzer. Temperature was varied between 2 degrees and 43 degrees , CO(2) concentration between 0.000% and 0.050% and light intensity between 40 ft-c and 1000 ft-c. In most ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.43.6.902

    authors: Jolliffe PA,Tregunna EB

    更新日期:1968-06-01 00:00:00

  • Decoys Untangle Complicated Redundancy and Reveal Targets of Circadian Clock F-Box Proteins.

    abstract::Eukaryotic circadian clocks utilize the ubiquitin proteasome system to precisely degrade clock proteins. In plants, the F-box-type E3 ubiquitin ligases ZEITLUPE (ZTL), FLAVIN-BINDING, KELCH REPEAT, F-BOX1 (FKF1), and LOV KELCH PROTEIN2 (LKP2) regulate clock period and couple the clock to photoperiodic flowering in res...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.18.00331

    authors: Lee CM,Feke A,Li MW,Adamchek C,Webb K,Pruneda-Paz J,Bennett EJ,Kay SA,Gendron JM

    更新日期:2018-07-01 00:00:00

  • Hormones and young leaves control development of cotyledonary buds in tomato seedlings.

    abstract::Hormonal and plant factors regulating the development of the inhibited cotyledonary buds of Lycopersicon esculentum Mill. cv. ;Fireball' seedlings were studied. Excision of the immature plumular leaves of 5- to 20- millimeter length significantly stimulated bud development after 2 to 4 days, but excision of leaves exc...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.62.2.276

    authors: Aung LH

    更新日期:1978-08-01 00:00:00

  • Water Stress Effects on Nitrogen Assimilation and Growth of Trifolium subterraneum L. Using Dinitrogen or Ammonium Nitrate.

    abstract::The relative effects of water stress on growth parameters of subterranean clover (Trifolium subterraneum L. cv. Woogenellup) dependent on either N(2) or 8 millimolar NH(4)NO(3) for N were examined. Whole-plant carbon exchange rate (CER), acetylene reduction (AR), dry matter production, and Kjeldahl N accumulation were...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.69.2.416

    authors: Dejong TM,Phillips DA

    更新日期:1982-02-01 00:00:00

  • Negative pressures produced in an artificial osmotic cell by extracellular freezing.

    abstract::A rigid artificial osmotic cell has been constructed using reverse osmosis membranes that were supported by metal grids from both sides to yield a high elastic modulus of the system. The cell could be subjected to changes of external water potential either by evaporation or by application of hypertonic solutions so th...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.91.4.1454

    authors: Zhu JJ,Steudle E,Beck E

    更新日期:1989-12-01 00:00:00

  • Vacuolar/Extravacuolar Distribution of Aminopeptidases in Giant Alga Chara australis and Partial Purification of One Such Enzyme.

    abstract::The presence of two major aminopeptidases (aminopeptidases I and II) in the giant alga Chara australis was shown using polyacrylamide gel electrophoresis. Partially purified aminopeptidase I had a molecular weight of about 120,000, hydrolyzed both leucine-beta-naphthylamide (pH optimum 6.0) and alanine-beta-naphthylam...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.84.3.720

    authors: Moriyasu Y,Sakano K,Tazawa M

    更新日期:1987-07-01 00:00:00

  • Two Class I Aldolases in the Green Alga Chara foetida (Charophyceae).

    abstract::Aldolase activity of Chara foetida (Braun) could be separated into a minor (peak I) and a major peak (peak II) by ion-exchange chromatography on DEAE-cellulose. Affinity chromatography on P-cellulose resulted in highly purified aldolase preparations with specific activities of 3.2 and 4.8 units per milligram protein a...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.87.1.78

    authors: Jacobshagen S,Schnarrenberger C

    更新日期:1988-05-01 00:00:00