The Arabidopsis embryo mutant schlepperless has a defect in the chaperonin-60alpha gene.

Abstract:

:We identified a T-DNA-generated mutation in the chaperonin-60alpha gene of Arabidopsis that produces a defect in embryo development. The mutation, termed schlepperless (slp), causes retardation of embryo development before the heart stage, even though embryo morphology remains normal. Beyond the heart stage, the slp mutation results in defective embryos with highly reduced cotyledons. slp embryos exhibit a normal apical-basal pattern and radial tissue organization, but they are morphologically retarded. Even though slp embryos are competent to transcribe two late-maturation gene markers, this competence is acquired more slowly as compared with wild-type embryos. slp embryos also exhibit a defect in plastid development-they remain white during maturation in planta and in culture. Hence, the overall developmental phenotype of the slp mutant reflects a lesion in the chloroplast that affects embryo development. The slp phenotype highlights the importance of the chaperonin-60alpha protein for chloroplast development and subsequently for the proper development of the plant embryo and seedling.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Apuya NR,Yadegari R,Fischer RL,Harada JJ,Zimmerman JL,Goldberg RB

doi

10.1104/pp.126.2.717

subject

Has Abstract

pub_date

2001-06-01 00:00:00

pages

717-30

issue

2

eissn

0032-0889

issn

1532-2548

journal_volume

126

pub_type

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