Abstract:
:Map-based cloning is an iterative approach that identifies the underlying genetic cause of a mutant phenotype. The major strength of this approach is the ability to tap into a nearly unlimited resource of natural and induced genetic variation without prior assumptions or knowledge of specific genes. One begins with an interesting mutant and allows plant biology to reveal what gene or genes are involved. Three major advances in the past 2 years have made map-based cloning in Arabidopsis fairly routine: sequencing of the Arabidopsis genome, the availability of more than 50,000 markers in the Cereon Arabidopsis Polymorphism Collection, and improvements in the methods used for detecting DNA polymorphisms. Here, we describe the Cereon Collection and show how it can be used in a generic approach to mutation mapping in Arabidopsis. We present the map-based cloning of the VTC2 gene as a specific example of this approach.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Jander G,Norris SR,Rounsley SD,Bush DF,Levin IM,Last RLdoi
10.1104/pp.003533subject
Has Abstractpub_date
2002-06-01 00:00:00pages
440-50issue
2eissn
0032-0889issn
1532-2548journal_volume
129pub_type
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