Abstract:
:Hydrogen peroxide (H(2)O(2)) induces increases, to different degrees, in transcripts, protein levels, and activity of the Ndh complex (EC 1.6.5.3). In the present work, we have compared the effects of relatively excess light, H(2)O(2), dimethylthiourea (a scavenger of H(2)O(2)), and/or EGTA (a Ca(2+) chelator) on the activity and protein levels of the Ndh complex of barley (Hordeum vulgare cv Hassan) leaf segments. The results show the involvement of H(2)O(2) in the modulation of both the protein level and activity of the Ndh complex and the participation of Ca(2+) mainly in the activity regulation of pre-existing protein. Changes in Ndh complex activity could not be explained only by changes in Ndh protein levels, suggesting posttranslational modifications. Hence, we investigate the possible phosphorylation of the Ndh complex both in thylakoids and in the immunopurified Ndh complex using monoclonal phosphoamino acid antibodies. We demonstrate that the Ndh complex is phosphorylated in vivo at threonine residue(s) of the NDH-F polypeptide and that the level of phosphorylation is closely correlated with the Ndh complex activity. The emerging picture is that full activity of the Ndh complex is reached by phosphorylation of its NDH-F subunit in a H(2)O(2)- and Ca(2+)-mediated action.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Lascano HR,Casano LM,Martín M,Sabater Bdoi
10.1104/pp.103.020321subject
Has Abstractpub_date
2003-05-01 00:00:00pages
256-62issue
1eissn
0032-0889issn
1532-2548journal_volume
132pub_type
杂志文章相关文献
PLANT PHYSIOLOGY文献大全abstract::Comparative genomics provides insight into the evolutionary dynamics that shape discrete sequences as well as whole genomes. To advance comparative genomics within the Brassicaceae, we have end sequenced 23,136 medium-sized insert clones from Boechera stricta, a wild relative of Arabidopsis (Arabidopsis thaliana). A s...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.105.073981
更新日期:2006-04-01 00:00:00
abstract::Nitrate reductase (NR; EC 1.6.6.1) activity increased at the beginning of the photoperiod in mature green maize (Zea mays L.) leaves as a result of increased enzyme protein level and protein dephosphorylation. In vitro experiments suggested that phosphorylation of maize leaf NR affected sensitivity to Mg2+ inhibition,...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.106.4.1667
更新日期:1994-12-01 00:00:00
abstract::Regulation of cortical microtubule reorganization is essential for plant cell survival under high salinity conditions. In response to salt stress, microtubules undergo rapid depolymerization followed by reassembly to form a new microtubule network that promotes cell survival; however, the upstream regulatory mechanism...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.17.01124
更新日期:2018-03-01 00:00:00
abstract::Using isopycnic sucrose gradients, we have ascertained the subcellular location of several enzymes involved in the processing of the N-linked oligosaccharides of glycoproteins in developing cotyledons of the common bean, Phaseolus vulgaris. All are localized in the endoplasmic reticulum (ER) or Golgi complex as determ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.85.3.741
更新日期:1987-11-01 00:00:00
abstract::A large proton leak not coupled to ATP synthesis (slip) occurs at alkaline pH through the chloroplast ATP synthase (Y. Evron, M. Avron [1990] Biochim Biophys Acta 1019: 115-120). The involvement of the ATP synthase [gamma]-subunit in the regulation of proton conductance was analyzed by measuring the effect of thiolalk...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.115.4.1549
更新日期:1997-12-01 00:00:00
abstract::Alternative oxidase (AOX) is encoded in small multigene families in plants. Functional analysis of the Arabidopsis (Arabidopsis thaliana) alternative oxidase 1c (AtAOX1c) promoter, an AOX gene not induced by oxidative stress, indicated that regulation of expression was complex, with the upstream promoter region contai...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.106.091819
更新日期:2007-04-01 00:00:00
abstract::Phaseolus vulgaris mitochondria incubated in sucrose swell rapidly upon the addition of phospholipase A. Bovine serum albumin inhibits the swelling. The release of free fatty acids as a result of phospholipase A action on the mitochondria is detected only in the presence of bovine serum albumin, which promotes the hyd...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.45.3.322
更新日期:1970-03-01 00:00:00
abstract::The fate of glutamine, the major source of nitrogen supplied to the developing maize endosperm, has been examined in endosperm tissues of corn caryopsis grown under sterile conditions. In the culture system, [U-(14)C]glutamine was included in the medium or was injected directly into the endosperm. Samples were harvest...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.77.3.520
更新日期:1985-03-01 00:00:00
abstract::The peptide snakin-2 (StSN2) has been isolated from potato (Solanum tuberosum cv Jaerla) tubers and found to be active (EC(50) = 1-20 microM) against fungal and bacterial plant pathogens. It causes a rapid aggregation of both Gram-positive and Gram-negative bacteria. The corresponding StSN2 cDNA encodes a signal seque...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.010685
更新日期:2002-03-01 00:00:00
abstract::Western blots of soluble protein from wheat, rice, and corn showed that ADPglucose pyrophosphorylase subunits have a size of 50 kilodaltons from endosperm tissue and 43 and 46 kilodaltons from leaf. Antisera to ADPglucose pyrophosphorylase precipitated in vitro translation products of 73 and 76 kilodaltons when leaf p...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.81.2.642
更新日期:1986-06-01 00:00:00
abstract::Hydroxyproline-rich glycoproteins (HRGPs) are a superfamily of plant cell wall proteins that function in diverse aspects of plant growth and development. This superfamily consists of three members: hyperglycosylated arabinogalactan proteins (AGPs), moderately glycosylated extensins (EXTs), and lightly glycosylated pro...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.110.156554
更新日期:2010-06-01 00:00:00
abstract::Insertional mutagenesis and gene silencing are efficient tools for the determination of gene function. In contrast to gain- or loss-of-function approaches, RNA interference (RNAi)-induced gene silencing can possibly silence multigene families and homoeologous genes in polyploids. This is of great importance for functi...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.106.084517
更新日期:2006-09-01 00:00:00
abstract::Glutathione reductase (EC 1.6.4.2) activity is present in spinach (Spinacia oleracea L.) chloroplasts. The pH dependence and substrate concentration for half-maximal rate are reported and a possible role in chloroplasts is proposed. ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.59.5.1011
更新日期:1977-05-01 00:00:00
abstract::Enriched peribacteroid membranes were prepared from Phaseolus vulgaris nodules and, in the presence of metleghemoglobin and H(2)O(2), membranal lipid peroxidation was observed. The initial rate of the reaction was low and increased with time. Ferrous leghemoglobin was unable to induce this peroxidation with H(2)O(2). ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.96.3.826
更新日期:1991-07-01 00:00:00
abstract::To dissect the expression of the psbB gene cluster of the Chlamydomonas reinhardtii chloroplast genome and to assess the role of the photosystem II H-phosphoprotein (PSII-H) in the biogenesis and/or stabilization of PSII, an aadA gene cassette conferring spectinomycin resistance was employed for mutagenesis. Disruptio...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.113.4.1359
更新日期:1997-04-01 00:00:00
abstract::The BEL1-like family of transcription factors is ubiquitous in plants and plays important roles in regulating development. They function in tandem with KNOTTED1 types to bind to a double TTGAC motif in the upstream sequence of target genes. StBEL5 of potato (Solanum tuberosum) functions as a mobile RNA signal that is ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.15.01314
更新日期:2016-01-01 00:00:00
abstract::The temperature response of net CO(2) assimilation rate (A), the rate of whole-chain electron transport, the activity and activation state of Rubisco, and the pool sizes of ribulose-1,5-bisphosphate (RuBP) and 3-phosphoglyceric acid (PGA) were assessed in sweet potato (Ipomoea batatas) grown under greenhouse condition...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.105.066233
更新日期:2005-10-01 00:00:00
abstract::At least two hydroxypyruvate reductases (HPRs), differing in specificity for NAD(P)H and (presumably) utilizing glyoxylate as a secondary substrate, were identified by fractionation of crude maize leaf extracts with ammonium sulfate. The NADH-preferring enzyme, which most probably represented peroxisomal HPR, was prec...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.91.1.278
更新日期:1989-09-01 00:00:00
abstract::The lv-1 mutant of pea (Pisum sativum L.) is deficient in responses regulated by phytochrome B (phyB) in other species but has normal levels of spectrally active phyB. We have characterized three further lv mutants (lv-2, lv-3, and lv-4), which are all elongated under red (R) and white light but are indistinguishable ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.108.2.525
更新日期:1995-06-01 00:00:00
abstract::SHORT-ROOT (SHR) and SCARECROW (SCR) are required for stem cell maintenance in the Arabidopsis (Arabidopsis thaliana) root meristem, ensuring its indeterminate growth. Mutation of SHR and SCR genes results in disorganization of the quiescent center and loss of stem cell activity, resulting in the cessation of root gro...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.110.158857
更新日期:2010-11-01 00:00:00
abstract::Phloem protein 2 (PP2) is one of the most abundant and enigmatic proteins in the phloem sap. Although thought to be associated with structural P-protein, PP2 is translocated in the assimilate stream where its lectin activity or RNA-binding properties can exert effects over long distances. Analyzing the diversity of th...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.013086
更新日期:2003-01-01 00:00:00
abstract::Auxin induction of the proliferation of Nicotiana tabacum (cv Xanthi) mesophyll protoplasts and of protoplast-derived cells was studied. The growth-promoting properties and cytotoxicities at high concentrations of IAA and naphthaleneacetic acid were strongly affected by cell density. The induction of growth by 2,4-dic...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.75.1.54
更新日期:1984-05-01 00:00:00
abstract::The influence of plant spacing and row orientation on spectral distribution of light received by growing soybean (Gylcine max [L.] Merr.) plants was measured under field conditions. Light absorption, reflection and transmission of individual leaves showed that most of the blue and red was absorbed while most of the fa...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.85.2.350
更新日期:1987-10-01 00:00:00
abstract::Tobacco (Nicotiana tabacum L. cv Xanthi) plants were exposed to toxic levels of zinc (Zn). Zn exposure resulted in toxicity signs in plants, and these damages were partly reduced by a calcium (Ca) supplement. Confocal imaging of intracellular Zn using Zinquin showed that Zn was preferentially accumulated in trichomes....
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.106.082743
更新日期:2006-07-01 00:00:00
abstract::Photosynthetically derived sugar provides carbon skeletons for lipid biosynthesis. We used mutants of Arabidopsis (Arabidopsis thaliana) and the expression of oleogenic factors to investigate relationships among sugar availability, lipid synthesis, and the accumulation of triacylglycerol (TAG) in leaf tissue. The adg1...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.17.00828
更新日期:2017-10-01 00:00:00
abstract::Injection of [(14)C]tryptophan into one seed in a soybean fruit resulted in recovery of radiolabel in a fraction that cochromatographed with indoleacetic acid (IAA) through three successive high performance liquid chromatography separations. Label was found in the putative IAA fraction in all of the fruit tissues samp...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.82.2.454
更新日期:1986-10-01 00:00:00
abstract::Apple (Malus × domestica) trees naturally produce an excess of fruitlets that negatively affect the commercial value of fruits brought to maturity and impact their capacity to develop flower buds the following season. Therefore, chemical thinning has become an important cultural practice, allowing the selective remova...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.114.236117
更新日期:2014-04-01 00:00:00
abstract::Like other chenopods, sugarbeets (Beta vulgaris L. cv Great Western D-2) accumulate glycine betaine when salinized; this may be an adaptive response to stress. The pathway of betaine synthesis in leaves of salinized (150-200 millimolar NaCl) sugarbeet plants was investigated by supplying [(14)C]formate, phosphoryl[(14...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.71.3.692
更新日期:1983-03-01 00:00:00
abstract::In angiosperms, auxin phytohormones play a crucial regulatory role in fruit initiation. The expression of auxin biosynthesis genes in ovules and placenta results in uncoupling of tomato (Solanum lycopersicum) fruit development from fertilization with production of parthenocarpic fruits. We have identified two newly de...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.108.131367
更新日期:2009-01-01 00:00:00
abstract::The occurrence of the complete orotic acid pathway for the biosynthesis de novo of pyrimidine nucleotides was demonstrated in the intact cells of roots excised from summer squash (Cucurbita pepo L. cv. Early Prolific Straightneck). Evidence that the biosynthesis of pyrimidine nucleotides proceeds via the orotate pathw...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.64.4.562
更新日期:1979-10-01 00:00:00