Lipid peroxidation in peribacteroid membranes from French-bean nodules.

Abstract:

:Enriched peribacteroid membranes were prepared from Phaseolus vulgaris nodules and, in the presence of metleghemoglobin and H(2)O(2), membranal lipid peroxidation was observed. The initial rate of the reaction was low and increased with time. Ferrous leghemoglobin was unable to induce this peroxidation with H(2)O(2). Thus, it appears that leghemoglobin (IV) is not the activated species involved in this process. Heme plays a role in this peroxidation and the hydroxyl radical is not an intermediate of the reaction. Lipid peroxidation in peribacteroid membranes was also observed in the presence of iron ions. A mixture of iron (III) and iron (II) produced a maximal peroxidation. Senescing nodule extracts were able to provoke membranal lipid peroxidation; they contained nonprotein-bound iron. Peribacteroid membranes were more sensitive than microsomes to peroxidation, as measured by malonaldehyde formation.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Puppo A,Herrada G,Rigaud J

doi

10.1104/pp.96.3.826

subject

Has Abstract

pub_date

1991-07-01 00:00:00

pages

826-30

issue

3

eissn

0032-0889

issn

1532-2548

journal_volume

96

pub_type

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