Arabidopsis HMA2, a divalent heavy metal-transporting P(IB)-type ATPase, is involved in cytoplasmic Zn2+ homeostasis.

Abstract:

:PIB-type ATPases transport heavy metal ions (Cu+, Cu2+, Zn2+, Cd2+, Co2+, etc.) across biological membranes. Several members of this subfamily are present in plants. Higher plants are the only eukaryotes where putative Zn(2+)-ATPases have been identified. We have cloned HMA2, a PIB-ATPase present in Arabidopsis (Arabidopsis thaliana), and functionally characterized this enzyme after heterologous expression in yeast (Saccharomyces cerevisiae). HMA2 is a Zn(2+)-dependent ATPase that is also activated by Cd2+ and, to a lesser extent, by other divalent heavy metals (Pb2+, Ni2+, Cu2+, and Co2+). The enzyme forms an acid-stable phosphorylated intermediate and is inhibited by vanadate. HMA2 interacts with Zn2+ and Cd2+ with high affinity (Zn2+ K(1/2) = 0.11 +/- 0.03 microm and Cd2+ K(1/2) = 0.031 +/- 0.007 microm). However, its activity is dependent on millimolar concentrations of Cys in the assay media. Zn2+ transport determinations indicate that the enzyme drives the outward transport of metals from the cell cytoplasm. Analysis of HMA2 mRNA suggests that the enzyme is present in all plant organs and transcript levels do not change in plants exposed to various metals. Removal of HMA2 full-length transcript results in Zn2+ accumulation in plant tissues. hma2 mutant plants also accumulate Cd2+ when exposed to this metal. These results suggest that HMA2 is responsible for Zn2+ efflux from the cells and therefore is required for maintaining low cytoplasmic Zn2+ levels and normal Zn2+ homeostasis.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Eren E,Argüello JM

doi

10.1104/pp.104.046292

subject

Has Abstract

pub_date

2004-11-01 00:00:00

pages

3712-23

issue

3

eissn

0032-0889

issn

1532-2548

pii

pp.104.046292

journal_volume

136

pub_type

杂志文章
  • Comparison between a Stable NaCl-Selected Nicotiana Cell Line and the Wild Type : K, Na, and Proline Pools as a Function of Salinity.

    abstract::An NaCl-resistant line has been developed from suspension-cultured tobacco cells (Nicotiana tabacum/gossii) by stepwise increases in the NaCl concentration in the medium. Resistance showed stability through at least 24 generations in the absence of added NaCl.Above an external NaCl concentration of 35 millimolar, prol...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.73.3.624

    authors: Watad AE,Reinhold L,Lerner HR

    更新日期:1983-11-01 00:00:00

  • Functional analysis of corn husk photosynthesis.

    abstract::The husk surrounding the ear of corn/maize (Zea mays) has widely spaced veins with a number of interveinal mesophyll (M) cells and has been described as operating a partial C(3) photosynthetic pathway, in contrast to its leaves, which use the C(4) photosynthetic pathway. Here, we characterized photosynthesis in maize ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.111.176495

    authors: Pengelly JJ,Kwasny S,Bala S,Evans JR,Voznesenskaya EV,Koteyeva NK,Edwards GE,Furbank RT,von Caemmerer S

    更新日期:2011-06-01 00:00:00

  • Calcium-Dependent Protein Phosphorylation May Mediate the Gibberellic Acid Response in Barley Aleurone

    abstract::Peptide substrates of well-defined protein kinases were microinjected into aleurone protoplasts of barley (Hordeum vulgare L. cv Himalaya) to inhibit, and therefore identify, protein kinase-regulated events in the transduction of the gibberellin (GA) and abscisic acid signals. Syntide-2, a substrate designed for Ca2+-...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.116.2.765

    authors: Ritchie S,Gilroy S

    更新日期:1998-02-01 00:00:00

  • Expression profile and cellular localization of maize Rpd3-type histone deacetylases during plant development.

    abstract::We analyzed the expression profile and cellular localization of the maize (Zea mays) Rpd3-type histone deacetylases genes ZmRpd3/101, ZmRpd3/102, and ZmRpd3/108 (indicated as ZmHDA101, ZmHDA102, and ZmHDA108 in the Plant Chromatin Database). This study shows that maize Rpd3 transcripts are present in all the organs an...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.103.025403

    authors: Varotto S,Locatelli S,Canova S,Pipal A,Motto M,Rossi V

    更新日期:2003-10-01 00:00:00

  • Concentrations of Indole-3-acetic Acid and Its Esters in Avena and Zea.

    abstract::An isotope-dilution method has been developed for the assay of free indole-3-acetic acid and ester indole-3-acetic acid as measured by indole-3-acetic acid liberated by mild alkaline hydrolysis. Application of this method to seedlings of Avena sativa and Zea mays indicates the upper limit of free indole-3-acetic acid ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.54.3.257

    authors: Bandurski RS,Schulze A

    更新日期:1974-09-01 00:00:00

  • Turnover of dhurrin in green sorghum seedlings.

    abstract::The turnover of dhurrin in green seedlings of Sorghum bicolor (Linn) Moench var Redland x Greenleaf, Sudan 70 has been investigated using glyphosate and pulse-labeling studies with (14)C-tyrosine and [(14)C]shikimic acid. The rate of dhurrin breakdown was 4.8 nanomoles per hour in the shoot and 1.4 nanomoles per hour ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.94.3.1219

    authors: Adewusi SR

    更新日期:1990-11-01 00:00:00

  • Role of RNA and protein synthesis in abscission.

    abstract::The cell separation aspect of abscission is thought to involve the action of specific cell wall degrading enzymes. Enzymes represent synthesis which in turn is preceded by the synthesis of specific RNA molecules, and it follows that inhibition of either of these processes would also block abscission. Since abscission ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:

    authors: Abeles FB

    更新日期:1968-09-01 00:00:00

  • Cytokinin-to-Auxin Ratios and Morphology of Shoots and Tissues Transformed by a Chimeric Isopentenyl Transferase Gene.

    abstract::Tissues transformed with the isopentenyl transferase (ipt) gene cloned from the T-DNA region of the Ti plasmid or with the ipt gene placed under the control of the cauliflower mosaic virus 35S promoter (35S-ipt) were analyzed for auxin and cytokinin. Free and total indole-3-acetic acid (IAA) levels in 35S-ipt-transfor...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.91.3.808

    authors: Smigocki AC,Owens LD

    更新日期:1989-11-01 00:00:00

  • Sorbitol metabolism and sink-source interconversions in developing apple leaves.

    abstract::In apple (Malus domestica Borkh.) sorbitol is the primary product of photosynthesis, the major translocated form of carbon, and a common fruit constituent and storage compound. Previous work on sorbitol metabolism has revealed a NADPH-dependent aldose 6-phosphate reductase (A6PR) in green tissues, and a NAD-dependent ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.70.2.335

    authors: Loescher WH,Marlow GC,Kennedy RA

    更新日期:1982-08-01 00:00:00

  • Immunocytolocalization of polygalacturonase in ripening tomato fruit.

    abstract::Using tissue blotting and immunocytolocalization, we have investigated the appearance and accumulation of polygalacturonase (PG) during tomato (Lycopersicon esculentum Mill.) fruit ripening. Results show that PG first appears in the collumella region followed by sequential appearance in the exopericarp and endopericar...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.90.1.17

    authors: Tieman DM,Handa AK

    更新日期:1989-05-01 00:00:00

  • Disruption of Ethylene Responses by Turnip mosaic virus Mediates Suppression of Plant Defense against the Green Peach Aphid Vector.

    abstract::Plants employ diverse responses mediated by phytohormones to defend themselves against pathogens and herbivores. Adapted pathogens and herbivores often manipulate these responses to their benefit. Previously, we demonstrated that Turnip mosaic virus (TuMV) infection suppresses callose deposition, an important plant de...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.15.00332

    authors: Casteel CL,De Alwis M,Bak A,Dong H,Whitham SA,Jander G

    更新日期:2015-09-01 00:00:00

  • Effects of Sink Removal on Photosynthesis and Senescence in Leaves of Soybean (Glycine max L.) Plants.

    abstract::Photosynthetic rate, ribulose 1,5-bisphosphate carboxylase activity, specific leaf weight, and leaf concentrations of carbohydrates, proteins, chlorophyll, and inorganic phosphate were determined periodically from midbloom until maturity in leaves of soybean plants (Glycine max L., var. Hodgson) from which reproductiv...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.61.3.394

    authors: Mondal MH,Brun WA,Brenner ML

    更新日期:1978-03-01 00:00:00

  • Structural and Functional Insights into a Lysine Deacylase in the Cyanobacterium Synechococcus sp. PCC 7002.

    abstract::Lys deacylases are essential regulators of cell biology in many contexts. Here, we have identified CddA (cyanobacterial deacetylase/depropionylase), a Lys deacylase enzyme expressed in the cyanobacterium Synechococcus sp. PCC 7002 that has both deacetylase and depropionylase activity. Loss of the gene cddA led to slow...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.20.00583

    authors: Liu X,Yang M,Liu Y,Ge F,Zhao J

    更新日期:2020-10-01 00:00:00

  • Heterogeneous distribution of glycosyltransferases in the endoplasmic reticulum of castor bean endosperm.

    abstract::Endoplasmic reticulum membranes stripped of attached ribosomes were isolated from homogenates of germinating castor bean (Ricinus communis L.) endosperm by sucrose density gradient centrifugation. The isolated endoplasmic reticulum fraction was further separated into two major membrane subfractions by centrifugation o...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.72.2.547

    authors: Conder MJ,Lord JM

    更新日期:1983-06-01 00:00:00

  • Characterization of Blue Light Signal Transduction Chains That Control Development and Maintenance of Sexual Competence in Chlamydomonas reinhardtii.

    abstract::Blue light induces the differentiation of Chlamydomonas reinhardtii pregametes to gametes. The light-induced conversion of pregametes to gametes is protein synthesis dependent and proceeds only after a lag phase. Upon incubation in the dark, gametes lost their mating ability, resulting in dark-inactivated gametes. Rei...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.115.3.1241

    authors: Pan JM,Haring MA,Beck CF

    更新日期:1997-11-01 00:00:00

  • Histone H2B monoubiquitination is involved in regulating the dynamics of microtubules during the defense response to Verticillium dahliae toxins in Arabidopsis.

    abstract::Histone H2B monoubiquitination (H2Bub) is being recognized as a regulatory mechanism that controls a range of cellular processes in plants, but the molecular mechanisms of H2Bub that are involved in responses to biotic stress are largely unknown. In this study, we used wild-type and H2Bub loss-of-function mutations of...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.113.234567

    authors: Hu M,Pei BL,Zhang LF,Li YZ

    更新日期:2014-04-01 00:00:00

  • Biphasic gene expression changes elicited by Phakopsora pachyrhizi in soybean correlate with fungal penetration and haustoria formation.

    abstract::Inoculation of soybean (Glycine max) plants with Phakopsora pachyrhizi, the causal organism of Asian soybean rust, elicits a biphasic response characterized by a burst of differential gene expression in the first 12 h. A quiescent period occurs from 24 to 48 h after inoculation, in which P. pachyrhizi continues to dev...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.111.181149

    authors: Schneider KT,van de Mortel M,Bancroft TJ,Braun E,Nettleton D,Nelson RT,Frederick RD,Baum TJ,Graham MA,Whitham SA

    更新日期:2011-09-01 00:00:00

  • Three 1-aminocyclopropane-1-carboxylate synthase genes regulated by primary and secondary pollination signals in orchid flowers.

    abstract::The temporal and spatial expression patterns of three 1-aminocyclopropane-1-carboxylate (ACC) synthase genes were investigated in pollinated orchid (Phalaenopsis spp.) flowers. Pollination signals initiate a cascade of development events in multiple floral organs, including the induction of ethylene biosynthesis, whic...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.116.1.419

    authors: Bui AQ,O'Neill SD

    更新日期:1998-01-01 00:00:00

  • Ca(2+)-activated anion channels and membrane depolarizations induced by blue light and cold in Arabidopsis seedlings.

    abstract::The activation of an anion channel in the plasma membrane of Arabidopsis thaliana hypocotyls by blue light (BL) is believed to be a signal-transducing event leading to growth inhibition. Here we report that the open probability of this particular anion channel depends on cytoplasmic Ca2+ ([Ca2+]cyt) within the concent...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.114.4.1327

    authors: Lewis BD,Karlin-Neumann G,Davis RW,Spalding EP

    更新日期:1997-08-01 00:00:00

  • Interactions between Mitochondria and Chloroplasts in Cells: I. Action of Cyanide and of 3-(3,4-Dichlorophenyl)-1,1-dimethylurea on the Spore of Funaria hygrometrica.

    abstract::The effects of cyanide and 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU) on photosynthesis and respiration of intact chlorophyllic moss (Funaria hygrometrica) spore was investigated. Thirty micromolar cyanide strongly inhibited dark respiration, was without effect on photosynthesis at high light intensities (above th...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.57.3.400

    authors: Chevallier D,Douce R

    更新日期:1976-03-01 00:00:00

  • Phytochrome Regulation of the Response to Exogenous Gibberellins by Epicotyls of Vigna sinensis.

    abstract::The elongation rate of cowpea epicotyls from whole cowpea (Vigna sinensis) seedlings and derooted and debladed plants (explants) increased after the main light period (8-hour duration) was extended with either continuous low intensity tungsten light or brief (5 minutes) far-red (FR) irradiation. This end-of-day FR eff...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.85.1.212

    authors: García-Martínez JL,Keith B,Bonner BA,Stafford AE,Rappaport L

    更新日期:1987-09-01 00:00:00

  • Nonadditive expression and parent-of-origin effects identified by microarray and allele-specific expression profiling of maize endosperm.

    abstract::Plant endosperm cells have a nuclear ratio of two maternal genomes to one paternal genome. This 2 to 1 dosage relationship provides a unique system for studying the additivity of gene expression levels in reciprocal hybrids. A combination of microarray profiling and allele-specific expression analysis was performed us...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.107.101428

    authors: Stupar RM,Hermanson PJ,Springer NM

    更新日期:2007-10-01 00:00:00

  • Cytokinins from the Moss Physcomitrella patens.

    abstract::Gametophore-over-producing mutants of the moss, Physcomitrella patens, when grown in liquid culture export high levels of cytokinin into their culture medium. The cytokinin produced by these mutants is postulated to account for their peculiar phenotype, that of mosses treated with exogenous cytokinin. N(6)-(Delta(2)-i...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.68.3.735

    authors: Wang TL,Horgan R,Cove D

    更新日期:1981-09-01 00:00:00

  • Studies of a novel cysteine sulfoxide lyase from Petiveria alliacea: the first heteromeric alliinase.

    abstract::A novel alliinase (EC 4.4.1.4) was detected and purified from the roots of the Amazonian medicinal plant Petiveria alliacea. The isolated enzyme is a heteropentameric glycoprotein composed of two alpha-subunits (68.1 kD each), one beta-subunit (56.0 kD), one gamma-subunit (24.8 kD), and one delta-subunit (13.9 kD). Th...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.109.142430

    authors: Musah RA,He Q,Kubec R,Jadhav A

    更新日期:2009-11-01 00:00:00

  • Effect of plant hormones on sucrose uptake by sugar beet root tissue discs.

    abstract::Abscisic acid (ABA), auxins, cytokinins, gibberellic acid, alone or in combination were tested for their effects on short-term sucrose uptake in sugar beet (Beta vulgaris cv USH-20) roots. The effect of ABA on active sucrose uptake varied from no effect to the more generally observed 1.4-to 3.0-fold stimulation. A rac...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.74.4.951

    authors: Saftner RA,Wyse RE

    更新日期:1984-04-01 00:00:00

  • Stachyose Synthesis in Source Leaf Tissues of the CAM Plant Xerosicyos danguyi H. Humb.

    abstract::Leaf tissues from Xerosicyos danguyi H. Humb., a succulent member of the Cucurbitaceae, were found to possess both galactinol synthase activity and the capacity for photosynthetic production of stachyose, the phloem transport oligosaccharide common to other nonsucculent cucurbits. The amounts of stachyose isolated fro...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.87.3.588

    authors: Madore MA,Mitchell DE,Boyd CM

    更新日期:1988-07-01 00:00:00

  • Electrogenic transport properties of growing Arabidopsis root hairs : the plasma membrane proton pump and potassium channels.

    abstract::Ion transport, measured using double-barreled micropipettes to obtain current-voltage relations, was examined in Arabidopsis thaliana root hairs that continued tip growth and cytoplasmic streaming after impalement with the micropipette. To do this required in situ measurements with no handling of the seedlings to avoi...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.97.4.1527

    authors: Lew RR

    更新日期:1991-12-01 00:00:00

  • The PsENOD12 Gene Is Expressed at Two Different Sites in Afghanistan Pea Pseudonodules Induced by Auxin Transport Inhibitors.

    abstract::A number of early nodulin genes are expressed in specific cell types as pea (Pisum sativum) root nodules develop. The Pisum sativum early nodulin PsENOD2 is detected only in the uninfected cells of the nodule parenchyma, whereas PsENOD12 is expressed at two spatially removed sites: in root hairs and adjacent cortical ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.100.4.1649

    authors: Scheres B,McKhann HI,Zalensky A,Löbler M,Bisseling T,Hirsch AM

    更新日期:1992-12-01 00:00:00

  • Termination of nutrient import and development of vein loading capacity in albino tobacco leaves.

    abstract::The sink-source conversion in developing leaves of tobacco (Nicotiana tabacum L.) was studied to determine whether import termination is caused by the onset of export or is related to achievement of positive carbon balance. Albino shoots were grown in vitro and grafted to detopped stems of green tobacco plants. Termin...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.76.1.45

    authors: Turgeon R

    更新日期:1984-09-01 00:00:00

  • Structure-activity relationship in the auxin activity of mono-substituted phenylacetic acids.

    abstract::The analysis of substituent constants for the lipophilic and electronic factors in the auxin activity of substituted phenylacetic acids in elongation of coleoptile segments shows that these factors parallel those for the phenoxyacetic acids but assign reactivity in growth promotion to the meta position of phenylacetic...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.42.11.1519

    authors: Muir RM,Fujita T,Hansch C

    更新日期:1967-11-01 00:00:00