Phytochrome Regulation of the Response to Exogenous Gibberellins by Epicotyls of Vigna sinensis.

Abstract:

:The elongation rate of cowpea epicotyls from whole cowpea (Vigna sinensis) seedlings and derooted and debladed plants (explants) increased after the main light period (8-hour duration) was extended with either continuous low intensity tungsten light or brief (5 minutes) far-red (FR) irradiation. This end-of-day FR effect was reversed by red (R) irradiation suggesting the involvement of phytochrome. These results confirm and extend those obtained previously with other species. Localization studies indicate the epicotyl to be the site of the photoreceptor. Treatment of cowpea seedlings with paclobutrazol, a gibberellin (GA) biosynthetic inhibitor, abolished the FR promoted epicotyl elongation, indicating a role for GAs in this process. There was no significant difference in epicotyl elongation rates of R plus FR irradiated explants treated with GA(1) or GA(20) and R irradiated explants treated with GA(1). However, R irradiation inhibited subsequent epicotyl elongation of GA(20) treated explants. Moreover, the observation, using GC-MS, that GA(1) and GA(20) are native GAs in cowpea lends support to the concept that phytochrome may control the conversion of endogenous GA(20) to GA(1) in cowpea.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

García-Martínez JL,Keith B,Bonner BA,Stafford AE,Rappaport L

doi

10.1104/pp.85.1.212

subject

Has Abstract

pub_date

1987-09-01 00:00:00

pages

212-6

issue

1

eissn

0032-0889

issn

1532-2548

journal_volume

85

pub_type

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