Role of oxygen fixation in hydroxyproline biosynthesis by etiolated seedlings.

Abstract:

:Etiolated maize and soybean seedlings were grown for several days in atmospheres enriched with O(18). Hydroxyproline subsequently isolated from the seedlings by column and thin-layer chromatography was labeled with excess O(18), but proline was not. Control experiments in which seedlings were grown in H(2)O(18) and unlabeled atmospheres demonstrated that neither proline nor hydroxyproline was labeled with excess O(18). It was concluded that oxygen fixation is an essential feature of hydroxyproline biosynthesis in these seedlings, and that the hydroxyl oxygen atom in hydroxyproline is derived from molecular oxygen and not from water; similar results have been reported previously for sycamore cell suspensions.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Stout ER,Fritz GJ

doi

10.1104/pp.41.2.197

subject

Has Abstract

pub_date

1966-02-01 00:00:00

pages

197-202

issue

2

eissn

0032-0889

issn

1532-2548

journal_volume

41

pub_type

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