Abstract:
:Etiolated maize and soybean seedlings were grown for several days in atmospheres enriched with O(18). Hydroxyproline subsequently isolated from the seedlings by column and thin-layer chromatography was labeled with excess O(18), but proline was not. Control experiments in which seedlings were grown in H(2)O(18) and unlabeled atmospheres demonstrated that neither proline nor hydroxyproline was labeled with excess O(18). It was concluded that oxygen fixation is an essential feature of hydroxyproline biosynthesis in these seedlings, and that the hydroxyl oxygen atom in hydroxyproline is derived from molecular oxygen and not from water; similar results have been reported previously for sycamore cell suspensions.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Stout ER,Fritz GJdoi
10.1104/pp.41.2.197subject
Has Abstractpub_date
1966-02-01 00:00:00pages
197-202issue
2eissn
0032-0889issn
1532-2548journal_volume
41pub_type
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