Cytochrome c is released in a reactive oxygen species-dependent manner and is degraded via caspase-like proteases in tobacco Bright-Yellow 2 cells en route to heat shock-induced cell death.

Abstract:

:To gain some insight into the mechanism of plant programmed cell death, certain features of cytochrome c (cyt c) release were investigated in heat-shocked tobacco (Nicotiana tabacum) Bright-Yellow 2 cells in the 2- to 6-h time range. We found that 2 h after heat shock, cyt c is released from intact mitochondria into the cytoplasm as a functionally active protein. Such a release did not occur in the presence of superoxide anion dismutase and catalase, thus showing that it depends on reactive oxygen species (ROS). Interestingly, ROS production due to xanthine plus xanthine oxidase results in cyt c release in sister control cultures. Maximal cyt c release was found 2 h after heat shock; later, activation of caspase-3-like protease was found to increase with time. Activation of this protease did not occur in the presence of ROS scavenger enzymes. The released cyt c was found to be progressively degraded in a manner prevented by either the broad-range caspase inhibitor (zVAD-fmk) or the specific inhibitor of caspase-3 (AC-DEVD-CHO), which have no effect on cyt c release. In the presence of these inhibitors, a significant increase in survival of the cells undergoing programmed cell death was found. We conclude that ROS can trigger release of cyt c, but do not cause cell death, which requires caspase-like activation.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Vacca RA,Valenti D,Bobba A,Merafina RS,Passarella S,Marra E

doi

10.1104/pp.106.078683

subject

Has Abstract

pub_date

2006-05-01 00:00:00

pages

208-19

issue

1

eissn

0032-0889

issn

1532-2548

pii

pp.106.078683

journal_volume

141

pub_type

杂志文章
  • Virus-based microRNA expression for gene functional analysis in plants.

    abstract::Traditional virus-induced gene silencing (VIGS) is a powerful virus-based short interfering RNA-mediated RNA silencing technique for plant functional genomics. Besides short interfering RNAs, microRNAs (miRNAs) have also been shown to regulate gene expression by RNA silencing in various organisms. However, plant virus...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.110.155796

    authors: Tang Y,Wang F,Zhao J,Xie K,Hong Y,Liu Y

    更新日期:2010-06-01 00:00:00

  • The glossyhead1 allele of ACC1 reveals a principal role for multidomain acetyl-coenzyme A carboxylase in the biosynthesis of cuticular waxes by Arabidopsis.

    abstract::A novel mutant of Arabidopsis (Arabidopsis thaliana), having highly glossy inflorescence stems, postgenital fusion in floral organs, and reduced fertility, was isolated from an ethyl methanesulfonate-mutagenized population and designated glossyhead1 (gsd1). The gsd1 locus was mapped to chromosome 1, and the causal gen...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.111.185132

    authors: Lü S,Zhao H,Parsons EP,Xu C,Kosma DK,Xu X,Chao D,Lohrey G,Bangarusamy DK,Wang G,Bressan RA,Jenks MA

    更新日期:2011-11-01 00:00:00

  • Extracellular calmodulin-induced stomatal closure is mediated by heterotrimeric G protein and H2O2.

    abstract::Extracellular calmodulin (ExtCaM) exerts multiple functions in animals and plants, but the mode of ExtCaM action is not well understood. In this paper, we provide evidence that ExtCaM stimulates a cascade of intracellular signaling events to regulate stomatal movement. Analysis of the changes of cytosolic free Ca2+ ([...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.104.047837

    authors: Chen YL,Huang R,Xiao YM,Lü P,Chen J,Wang XC

    更新日期:2004-12-01 00:00:00

  • Parthenocarpic Fruit Development from Grafted Ovaries of Cucuminis sativus L.

    abstract::Reciprocal cleft and pistillate floral bud grafts were made between parthenocarpic ;Fertilla' and nonparthenocarpic ;MSU 713-5' cucumber (Cucumis sativus L.) lines to localize the site for stimulation of parthenocarpic fruit set. No fruit set on ;MSU 713-5' controls, scion grafted to ;Fertilla,' or rootstock with ;Fer...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.55.6.1107

    authors: Cantliffe DJ,Phatak SC

    更新日期:1975-06-01 00:00:00

  • Sugar-regulated expression of a putative hexose transport gene in grape.

    abstract::Different lengths of the promoter of grape (Vitis vinifera) VvHT1 (Hexose Transporter 1) gene, which encodes a putative hexose transporter expressed during the ripening of grape, have been transcriptionally fused to the beta-glucuronidase reporter gene. In transgenic tobacco (Nicotiana tabacum) transformed with these ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.009522

    authors: Atanassova R,Leterrier M,Gaillard C,Agasse A,Sagot E,Coutos-Thévenot P,Delrot S

    更新日期:2003-01-01 00:00:00

  • Stomatal Blue Light Response Is Present in Early Vascular Plants.

    abstract::Light is a major environmental factor required for stomatal opening. Blue light (BL) induces stomatal opening in higher plants as a signal under the photosynthetic active radiation. The stomatal BL response is not present in the fern species of Polypodiopsida. The acquisition of a stomatal BL response might provide co...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.15.00134

    authors: Doi M,Kitagawa Y,Shimazaki K

    更新日期:2015-10-01 00:00:00

  • Reduction of Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase by Antisense RNA in the C4 Plant Flaveria bidentis Leads to Reduced Assimilation Rates and Increased Carbon Isotope Discrimination.

    abstract::Transgenic Flaveria bidentis (a C4 species) plants with an antisense gene directed against the mRNA of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) were used to examine the relationship between the CO2 assimilation rate, Rubisco content, and carbon isotope discrimination. Reduction in the amount of Rubisc...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.113.2.469

    authors: Von Caemmerer S,Millgate A,Farquhar GD,Furbank RT

    更新日期:1997-02-01 00:00:00

  • BpNAC012 Positively Regulates Abiotic Stress Responses and Secondary Wall Biosynthesis.

    abstract::NAC (NAM, ATAF1/2, and CUC2) transcription factors play important roles in plant biological processes and stress responses. Here, we characterized the functional roles of BpNAC012 in white birch (Betula platyphylla). We found that BpNAC012 serves as a transcriptional activator. Gain- and loss-of-function analyses reve...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.18.01167

    authors: Hu P,Zhang K,Yang C

    更新日期:2019-02-01 00:00:00

  • Plant Signaling 2000. Cross talk among geneticists, physiologists, and ecologists.

    abstract::Plants respond in complex ways to their environment, to their internal physiological status, and to the activity of other plants, pathogens, herbivores, and organisms. Plant Signaling 2000, a symposium sponsored by the Penn State Intercollege Graduate Program in Plant Physiology (May 18-20, 2000), explored the machine...

    journal_title:Plant physiology

    pub_type:

    doi:10.1104/pp.124.2.499

    authors: Cosgrove DJ,Gilroy S,Kao TH,Ma H,Schultz JC

    更新日期:2000-10-01 00:00:00

  • Enhanced Disease Susceptibility1 Mediates Pathogen Resistance and Virulence Function of a Bacterial Effector in Soybean.

    abstract::Enhanced disease susceptibility1 (EDS1) and phytoalexin deficient4 (PAD4) are well-known regulators of both basal and resistance (R) protein-mediated plant defense. We identified two EDS1-like (GmEDS1a/GmEDS1b) proteins and one PAD4-like (GmPAD4) protein that are required for resistance signaling in soybean (Glycine m...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.114.242495

    authors: Wang J,Shine MB,Gao QM,Navarre D,Jiang W,Liu C,Chen Q,Hu G,Kachroo A

    更新日期:2014-07-01 00:00:00

  • Selective Inhibition of Active Uptake of Sucrose into Plasma Membrane Vesicles by Polyclonal Sera Directed against a 42 Kilodalton Plasma Membrane Polypeptide.

    abstract::Several polyclonal sera were raised in rabbits and in mice against putative sucrose carrier proteins, i.e. a 42 kilodalton (O Gallet, R Lemoine, C Larsson, S Delrot [1989] Biochim Biophys Acta 978: 56-64) and a 62 kD (KG Ripp, PV Viitanen, WD Hitz, VR Fransceschi [1988] Plant Physiol 88: 1435-1445) polypeptide of the ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.98.1.17

    authors: Gallet O,Lemoine R,Gaillard C,Larsson C,Delrot S

    更新日期:1992-01-01 00:00:00

  • Resistance to Botrytis cinerea in sitiens, an abscisic acid-deficient tomato mutant, involves timely production of hydrogen peroxide and cell wall modifications in the epidermis.

    abstract::Plant defense mechanisms against necrotrophic pathogens, such as Botrytis cinerea, are considered to be complex and to differ from those that are effective against biotrophs. In the abscisic acid-deficient sitiens tomato (Solanum lycopersicum) mutant, which is highly resistant to B. cinerea, accumulation of hydrogen p...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.107.099226

    authors: Asselbergh B,Curvers K,Franca SC,Audenaert K,Vuylsteke M,Van Breusegem F,Höfte M

    更新日期:2007-08-01 00:00:00

  • Cucumber seedling indoleacetaldehyde oxidase.

    abstract::Extracts of light-grown Cucumis sativus L. seedlings catalyzed the oxidation of indole-3-acetaldehyde to indole-3-acetic acid. No added cofactors were required. Inhibitor studies indicated that the enzyme is a metalloflavoprotein. While indole-3-aldehyde, benzaldehyde, and phenylacetaldehyde partially inhibited the ox...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.61.1.107

    authors: Bower PJ,Brown HM,Purves WK

    更新日期:1978-01-01 00:00:00

  • Correction of Freezing Artifacts in the Application of Electron Paramagnetic Resonance for Quantification of the Reduction of Ferric Hydroxamate by Chlorella vulgaris.

    abstract::Electron paramagnetic resonance has been used to quantitate reduction of ferric ion bound to hydroxamate siderophores by iron-stressed fungi (DJ Ecker et al. 1982 J Biol Chem 257:8623-8626) and by iron-stressed Chlorella vulgaris (ATCC 11468) (FCT Allnutt, WD Bonner 1984 J Plant Nutr 7: 427-435). Application of this t...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.83.4.739

    authors: Allnutt FC,Bonner WD

    更新日期:1987-04-01 00:00:00

  • Regulation of Pyrimidine Biosynthesis in Intact Cells of Cucurbita pepo.

    abstract::The occurrence of the complete orotic acid pathway for the biosynthesis de novo of pyrimidine nucleotides was demonstrated in the intact cells of roots excised from summer squash (Cucurbita pepo L. cv. Early Prolific Straightneck). Evidence that the biosynthesis of pyrimidine nucleotides proceeds via the orotate pathw...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.64.4.562

    authors: Lovatt CJ,Albert LS

    更新日期:1979-10-01 00:00:00

  • Nicotinamide Adenine Dinucleotide-dependent Proline Dehydrogenase in Chlorella.

    abstract::An NAD-linked dehydrogenase from Chlorella pyrenoidosa Chick catalyzing the conversion of l-proline to Delta(1)-pyrroline-5-carboxylic acid was partially purified. Delta(1)-Pyrroline-5-carboxylic acid was identified as the product by co-chromatography of it and its o-aminobenzaldehyde derivative with authentic compoun...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.53.3.440

    authors: McNamer AD,Stewart CR

    更新日期:1974-03-01 00:00:00

  • Compartmentation in Vicia faba Leaves: I. Kinetics of C in the Tissues following Pulse Labeling.

    abstract::Leaflets of Vicia faba were pulse-labeled with (14)CO(2) to follow the subsequent movement of photosynthate between leaf tissues. Samples were taken during a (12)CO(2) chase, quick frozen, freeze-substituted, and embedded in methacrylate. Paradermal sections provided tissue samples consisting only of upper epidermis, ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.55.4.699

    authors: Outlaw WH,Fisher DB

    更新日期:1975-04-01 00:00:00

  • Manipulation of in vivo sorbitol production alters boron uptake and transport in tobacco

    abstract::Recent evidence that some species can retranslocate boron as complexes with sugar alcohols in the phloem suggests a possible mechanism for enhancing boron efficiency. We investigated the relationship between sugar alcohol (sorbitol) content, boron uptake and distribution, and translocation of foliar-applied, isotopica...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.119.2.735

    authors: Bellaloui N,Brown PH,Dandekar AM

    更新日期:1999-02-01 00:00:00

  • Distinct patterns of genetic variation alter flowering responses of Arabidopsis accessions to different daylengths.

    abstract::Many plants flower in response to seasonal changes in daylength. This response often varies between accessions of a single species. We studied the variation in photoperiod response found in the model species Arabidopsis (Arabidopsis thaliana). Seventy-two accessions were grown under six daylengths varying in 2-h inter...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.109.140772

    authors: Giakountis A,Cremer F,Sim S,Reymond M,Schmitt J,Coupland G

    更新日期:2010-01-01 00:00:00

  • A new C-type cyclin-dependent kinase from tomato expressed in dividing tissues does not interact with mitotic and G1 cyclins.

    abstract::Cyclin-dependent kinases (CDKs) form a conserved superfamily of eukaryotic serine-threonine protein kinases whose activity requires the binding of a cyclin protein. CDKs are involved in many aspects of cell biology and notably in the regulation of the cell cycle. Three cDNAs encoding a C-type CDK, and a member of each...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.126.4.1403

    authors: Joubès J,Lemaire-Chamley M,Delmas F,Walter J,Hernould M,Mouras A,Raymond P,Chevalier C

    更新日期:2001-08-01 00:00:00

  • Pyrophosphate Fructose-6-P 1-Phosphotransferase from Tomato Fruit : Evidence for Change during Ripening.

    abstract::Three forms of pyrophosphate fructose-6-phosphate 1-phosphotransferase (PFP) were purified from both green and red tomato (Lycopersicon esculentum) fruit: (a) a classical form (designated Q(2)) containing alpha- (66 kilodalton) and beta- (60 kilodalton) subunits; (b) a form (Q(1)) containing a beta-doublet subunit; an...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.94.2.499

    authors: Wong JH,Kiss F,Wu MX,Buchanan BB

    更新日期:1990-10-01 00:00:00

  • Tonoplast intrinsic proteins AtTIP2;1 and AtTIP2;3 facilitate NH3 transport into the vacuole.

    abstract::While membrane transporters mediating ammonium uptake across the plasma membrane have been well described at the molecular level, little is known about compartmentation and cellular export of ammonium. (The term ammonium is used to denote both NH3 and NH4+ and chemical symbols are used when specificity is required.) W...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.104.051268

    authors: Loqué D,Ludewig U,Yuan L,von Wirén N

    更新日期:2005-02-01 00:00:00

  • Synthesis of chymotrypsin inhibitor I protein in potato leaflets induced by detachment.

    abstract::Chymotrypsin inhibitor I is a protein that can be induced to accumulate in potato leaflets within hr in the light when leaflets are detached from intact plants and supplied with water. This increase in inhibitor I is not accompanied by an increase in all proteins in the detached leaflets. The accumulation is polar, pr...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.43.11.1859

    authors: Ryan CA

    更新日期:1968-11-01 00:00:00

  • Host-Pathogen Interactions : XXIV. Fragments Isolated from Suspension-Cultured Sycamore Cell Walls Inhibit the Ability of the Cells to Incorporate [C]Leucine into Proteins.

    abstract::A bioassay to measure the incorporation of [(14)C]leucine into acid-precipitable polymers of suspension-cultured sycamore (Acer pseudoplatanus L.) cells is described. Using this assay, cell wall fragments solubilized from sycamore cell walls by partial acid hydrolysis are shown to contain components that inhibit the i...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.72.3.864

    authors: Yamazaki N,Fry SC,Darvill AG,Albersheim P

    更新日期:1983-07-01 00:00:00

  • Isotope ratios of cellulose from plants having different photosynthetic pathways.

    abstract::Hydrogen and carbon isotope ratios of cellulose nitrate and oxygen isotope ratios of cellulose from C(3), C(4), and Crassulacean acid metabolism (CAM) plants were determined for plants growing within a small area in Val Verde County, Texas. Plants having CAM had distinctly higher deuterium/hydrogen (D/H) ratios than p...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.74.3.557

    authors: Sternberg LO,Deniro MJ,Johnson HB

    更新日期:1984-03-01 00:00:00

  • Sodium and potassium absorption by bean stem tissue.

    abstract::The effect of various periods of pretreatment in CaSO(4) solutions (aging) on the absorption of Na and K by bean stem slices was investigated. Freshly sliced tissue absorbed Na over the entire range of concentrations studied (0.02-50 mm). Potassium absorption by fresh tissue was nil at concentrations below 0.5 mm but ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.44.4.547

    authors: Rains DW

    更新日期:1969-04-01 00:00:00

  • Osmotic adjustment by intact isolated chloroplasts in response to osmotic stress and its effect on photosynthesis and chloroplast volume.

    abstract::Spinach leaf chloroplasts isolated in isotonic media (330 millimolar sorbitol, -1.0 megapascals osmotic potential) had optimum rates of photosynthesis when assayed at -1.0 megapascals. When chloroplasts were isolated in hypertonic media (720 millimolar sorbitol, -2.0 megapascals osmotic potential) the optimum osmotic ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.79.4.996

    authors: Robinson SP

    更新日期:1985-12-01 00:00:00

  • Regulation of Nicotine Biosynthesis by an Endogenous Target Mimicry of MicroRNA in Tobacco.

    abstract::The interaction between noncoding endogenous target mimicry (eTM) and its corresponding microRNA (miRNA) is a newly discovered regulatory mechanism and plays pivotal roles in various biological processes in plants. Tobacco (Nicotiana tabacum) is a model plant for studying secondary metabolite alkaloids, of which nicot...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.15.00649

    authors: Li F,Wang W,Zhao N,Xiao B,Cao P,Wu X,Ye C,Shen E,Qiu J,Zhu QH,Xie J,Zhou X,Fan L

    更新日期:2015-10-01 00:00:00

  • Ion transport processes in corn mitochondria : I. Effect of the local anesthetic dibucaine.

    abstract::The local anesthetic dibucaine inhibited respiration-dependent contraction mediated by the K(+)/H(+) antiport system of isolated corn mitochondria. Respiration declined concurrently. Nigericin, an exogenous K(+)/H(+) exchanger, restored ion efflux in dibucaine-blocked corn mitochondria. It was concluded that dibucaine...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.77.4.909

    authors: Klein RR,Koeppe DE

    更新日期:1985-04-01 00:00:00

  • Uptake and Utilization of Xylem-borne Amino Compounds by Shoot Organs of a Legume.

    abstract::Amino compounds representative of the major N solutes of xylem sap were pulse-fed (10 to 20 minutes) singly in (14)C-labeled form to cut transpiring shoots of white lupin (Lupinus albus L.). (14)C distribution was studied by autoradiography and radioassays of phloem sap, leaflet tissues, and shoot parts harvested at i...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.63.6.1076

    authors: McNeil DL,Atkins CA,Pate JS

    更新日期:1979-06-01 00:00:00