Calcium-Dependent Protein Phosphorylation May Mediate the Gibberellic Acid Response in Barley Aleurone

Abstract:

:Peptide substrates of well-defined protein kinases were microinjected into aleurone protoplasts of barley (Hordeum vulgare L. cv Himalaya) to inhibit, and therefore identify, protein kinase-regulated events in the transduction of the gibberellin (GA) and abscisic acid signals. Syntide-2, a substrate designed for Ca2+- and calmodulin (CaM)-dependent kinases, selectively inhibited the GA response, leaving constitutive and abscisic acid-regulated events unaffected. Microinjection of syntide did not affect the GA-induced increase in cytosolic [Ca2+], suggesting that it inhibited GA action downstream of the Ca2+ signal. When photoaffinity-labeled syntide-2 was electroporated into protoplasts and cross-linked to interacting proteins in situ, it selectively labeled proteins of approximately 30 and 55 kD. A 54-kD, soluble syntide-2 phosphorylating protein kinase was detected in aleurone cells. This kinase was activated by Ca2+ and was CaM independent, but was inhibited by the CaM antagonist N-(6-aminohexyl)-5-chloro-1-naphthalene-sulfonamide (250 mum), suggesting that it was a CaM-domain protein kinase-like activity. These results suggest that syntide-2 inhibits the GA response of the aleurone via an interaction with this kinase, implicating the 54-kD kinase as a Ca2+-dependent regulator of the GA response in these cells.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Ritchie S,Gilroy S

doi

10.1104/pp.116.2.765

subject

Has Abstract

pub_date

1998-02-01 00:00:00

pages

765-76

issue

2

eissn

0032-0889

issn

1532-2548

journal_volume

116

pub_type

杂志文章
  • Effect of asymmetric auxin application on Helianthus hypocotyl curvature.

    abstract::Indole-3-acetic acid was applied asymmetrically to the hypocotyls of sunflower (Helianthus annuus L.) seedlings. After 5 hours on a clinostat, auxin gradients as small as 1 to 1.3 produced substantial (more than 60 degrees) hypocotyl curvature. This result suggests the asymmetric growth underlying hypocotyl gravitro...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.91.2.466

    authors: Migliaccio F,Rayle DL

    更新日期:1989-01-01 00:00:00

  • Multisite inhibition of Pinus pinea isocitrate lyase by phosphate.

    abstract::Our results show that the phosphate ion is a nonlinear competitive inhibitor of Pinus pinea isocitrate lyase. In addition, this compound induces a sigmoidal response of the enzyme, which usually exhibits standard Michaelis-Menten kinetics. This peculiar behavior of P. pinea isocitrate lyase could be explained by a dim...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.124.3.1131

    authors: Ranaldi F,Vanni P,Giachetti E

    更新日期:2000-11-01 00:00:00

  • AtNUDT7, a negative regulator of basal immunity in Arabidopsis, modulates two distinct defense response pathways and is involved in maintaining redox homeostasis.

    abstract::Plants have evolved complicated regulatory systems to control immune responses. Both positive and negative signaling pathways interplay to coordinate development of a resistance response with the appropriate amplitude and duration. AtNUDT7, a Nudix domain-containing protein in Arabidopsis (Arabidopsis thaliana) that h...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.107.103374

    authors: Ge X,Li GJ,Wang SB,Zhu H,Zhu T,Wang X,Xia Y

    更新日期:2007-09-01 00:00:00

  • Amyloplast sedimentation dynamics in maize columella cells support a new model for the gravity-sensing apparatus of roots.

    abstract::Quantitative analysis of statolith sedimentation behavior was accomplished using videomicroscopy of living columella cells of corn (Zea mays) roots, which displayed no systematic cytoplasmic streaming. Following 90 degrees rotation of the root, the statoliths moved downward along the distal wall and then spread out al...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.125.2.1045

    authors: Yoder TL,Zheng HQ,Todd P,Staehelin LA

    更新日期:2001-02-01 00:00:00

  • Peroxidase Release Induced by Ozone in Sedum album Leaves: Involvement of Ca.

    abstract::The effect of ozone was studied on the peroxidase activity from various compartments of Sedum album leaves (epidermis, intercellular fluid, residual cell material, and total cell material). The greatest increase following a 2-hour ozone exposure (0.4 microliters O(3) per liter) was observed in extracellular peroxidase...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.74.4.846

    authors: Castillo FJ,Penel C,Greppin H

    更新日期:1984-04-01 00:00:00

  • Chloroplast Ultrastructure in Mutant Strains of Chlamydomonas reinhardi Lacking Components of the Photosynthetic Apparatus.

    abstract::The fine structure of the chloroplast of wild-type and 9 photosynthetic mutant strains of Chlamydomonas reinhardi is described. The chloroplast phenotypes of the mutant strains are clearly distinct from the wild type in all but 2 cases. Moreover, strains with similar photosynthetic disabilities have structurally simil...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.44.7.990

    authors: Goodenough UW,Levine RP

    更新日期:1969-07-01 00:00:00

  • Sensitivity to Flg22 is modulated by ligand-induced degradation and de novo synthesis of the endogenous flagellin-receptor FLAGELLIN-SENSING2.

    abstract::FLAGELLIN-SENSING2 (FLS2) is the plant cell surface receptor that perceives bacterial flagellin or flg22 peptide, initiates flg22-signaling responses, and contributes to bacterial growth restriction. Flg22 elicitation also leads to ligand-induced endocytosis and degradation of FLS2 within 1 h. Why plant cells remove t...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.113.229179

    authors: Smith JM,Salamango DJ,Leslie ME,Collins CA,Heese A

    更新日期:2014-01-01 00:00:00

  • Identification and characterization of nucleotide-binding site-leucine-rich repeat genes in the model plant Medicago truncatula.

    abstract::The nucleotide-binding site (NBS)-Leucine-rich repeat (LRR) gene family accounts for the largest number of known disease resistance genes, and is one of the largest gene families in plant genomes. We have identified 333 nonredundant NBS-LRRs in the current Medicago truncatula draft genome (Mt1.0), likely representing ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.107.104588

    authors: Ameline-Torregrosa C,Wang BB,O'Bleness MS,Deshpande S,Zhu H,Roe B,Young ND,Cannon SB

    更新日期:2008-01-01 00:00:00

  • Immunological Comparisons of Chymotrypsin Inhibitor I among Several Genera of the Solanaceae.

    abstract::Microcomplement fixation was employed to compare the immunological differences that occur between purified inhibitor I from potato tubers, the four purified protomers that comprise it, and inhibitor I from tuber and leaf extracts. Total inhibitors of chymotrypsin and trypsin in leaves of seven genera of the Solanaceae...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.50.5.627

    authors: Gurusiddaiah S,Kuo T,Ryan CA

    更新日期:1972-11-01 00:00:00

  • Agrobacterium tumefaciens Site Attachment as a Necessary Prerequisite for Crown Gall Tumor Formation on Potato Discs.

    abstract::The infectivity of Agrobacterium tumefaciens strain B6 was inhibited about 50% when these bacteria were inoculated on potato discs with equal viable cell counts of a weakly virulent strain of A. tumefaciens (B-48) or autoclaved strains of B6 or B-48. Inhibition by B-48 or autoclaved B6 could still be obtained when the...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.61.6.1031

    authors: Glogowski W,Galsky AG

    更新日期:1978-06-01 00:00:00

  • Growth Response of Halophila engelmanni Aschers. to Sulfide, Copper, and Organic Nitrogen in Marine Sediments.

    abstract::Growth response of the seagrass Halophila engelmanni Aschers. was studied using defined culture techniques. When added to the rhizosphere, nutrient-seawater medium supported vigorous plant growth, if substantial Cu (10 times that in modified Hoagland solution) plus organic N were present. H(2)S up to 0.2 millimolar in...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.71.4.975

    authors: Pulich WM

    更新日期:1983-04-01 00:00:00

  • Ozone-Induced Alterations in the Accumulation of Newly Synthesized Proteins in Leaves of Maize.

    abstract::We examined the response of leaves of 3-week-old maize (Zea mays L.) to short-term (5 h) fumigation with O3-enriched air (0, 0.12, 0.24, or 0.36 [mu]L/L). Older leaves and leaf tissue developed more severe visible damage at higher external O3 concentrations. To investigate the immediate effect of O3 exposure on the ac...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.108.2.777

    authors: Pino ME,Mudd JB,Bailey-Serres J

    更新日期:1995-06-01 00:00:00

  • Preparation of Membrane Vesicles Enriched in ATP-Dependent Proton Transport from Suspension Cultures of Tomato Cells.

    abstract::Membranes enriched in ATP-dependent proton transport were prepared from suspension cultures of tomato cells (Lycopersicon esculentum Mill cv VF36). Suspension cultures were a source of large quantities of membranes from rapidly growing, undifferentiated cells. Proton transport activity was assayed as quench of acridin...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.77.1.69

    authors: Dupont FM,Zabala Mde G

    更新日期:1985-01-01 00:00:00

  • Differential metal selectivity and gene expression of two zinc transporters from rice.

    abstract::Zinc is an essential mineral for a wide variety of physiological and biochemical processes. To understand zinc transport in cereals, we identified putative zinc transporters in gene databases. Three full-length cDNAs were identified and characterized from rice (Oryza sativa). Two of the cDNAs partially complemented a ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.103.026815

    authors: Ramesh SA,Shin R,Eide DJ,Schachtman DP

    更新日期:2003-09-01 00:00:00

  • Extremely low frequency resistance fluctuations of chara membrane.

    abstract::The membrane resistivity of resting characean cells was measured and found to fluctuate unpredictably about its resting level. The amplitude of these fluctuations relative to the mean resistivity was normally somewhat larger than the same ratio for the vacuolar voltage; further the resistivity and voltage fluctuations...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.79.3.723

    authors: Gokhale AV,Pickard WF

    更新日期:1985-11-01 00:00:00

  • Identification of a Transport Mechanism for NH4+ in the Symbiosome Membrane of Pea Root Nodules.

    abstract::Symbiosome membrane vesicles, facing bacteroid-side-out, were purified from pea (Pisum sativum L.) root nodules and used to study NH4+ transport across the membrane by recording vesicle uptake of the NH4+ analog [14C]methylamine (MA). Membrane potentials ([delta][psi]) were imposed on the vesicles using K+ concentrati...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.115.2.519

    authors: Mouritzen P,Rosendahl L

    更新日期:1997-10-01 00:00:00

  • Photosynthesis in the Higher Plant, Vicia faba: III. Serine, a Precursor of the Tricarboxylic Acid Cycle.

    abstract::Evidence is presented to support the hypothesis that serine, rather than 3-phosphoglycerate of the Calvin cycle, is a precursor of the tricarboxylic acid cycle during photosynthesis by the higher plant, Vicia faba. Identification of the serine intermediate is based upon a unique C(1) > C(2) > C(3) isotope distribution...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.53.3.491

    authors: Kent SS,Pinkerton FD,Strobel GA

    更新日期:1974-03-01 00:00:00

  • Analysis of the Distribution of Potassium-stimulated Adenosine Triphosphatase Activity in Soybean Root.

    abstract::The distribution of activity of a potassium-stimulated ATPase associated with the plasma membrane was determined in 4-day-old soybean roots. Changes in protein-based specific activity of the enzyme coincided with developmental changes in the root. Activity was low in the region of the root cap, increased to a maximum ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.63.6.1187

    authors: Travis RL,Geng S,Berkowitz RL

    更新日期:1979-06-01 00:00:00

  • Nucleoid-enriched proteomes in developing plastids and chloroplasts from maize leaves: a new conceptual framework for nucleoid functions.

    abstract::Plastids contain multiple copies of the plastid chromosome, folded together with proteins and RNA into nucleoids. The degree to which components of the plastid gene expression and protein biogenesis machineries are nucleoid associated, and the factors involved in plastid DNA organization, repair, and replication, are ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.111.188474

    authors: Majeran W,Friso G,Asakura Y,Qu X,Huang M,Ponnala L,Watkins KP,Barkan A,van Wijk KJ

    更新日期:2012-01-01 00:00:00

  • Induction of the Root Cell Plasma Membrane Ferric Reductase (An Exclusive Role for Fe and Cu).

    abstract::Induction of ferric reductase activity in dicots and nongrass monocots is a well-recognized response to Fe deficiency. Recent evidence has shown that Cu deficiency also induces plasma membrane Fe reduction. In this study we investigated whether other nutrient deficiencies could also induce ferric reductase activity in...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.114.3.1061

    authors: Cohen CK,Norvell WA,Kochian LV

    更新日期:1997-07-01 00:00:00

  • Movement of Kinetin and Gibberellic Acid in Leaf Petioles during Water Stress-induced Abscission in Cotton.

    abstract::Movement of [(14)C]kinetin and [(14)C]gibberellic acid was examined in cotton (Gossypium hirsutum L.) cotyledonary petiole sections independent of label uptake or exit from the tissue. Sections 20 millimeters in length were taken from well watered, stressed, and poststressed plants. Transport capacity was determined u...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.63.1.152

    authors: Davenport TL,Jordan WR,Morgan PW

    更新日期:1979-01-01 00:00:00

  • Photocontrol of the Germination of Onoclea Spores: II. Analysis of Germination Processes by Means of Anaerobiosis.

    abstract::The oxygen requirements during the three phases of photoinduced germination of Onoclea sensibilis L. spores were analyzed by temporarily applying nitrogen atmosphere. The dark preinduction phase, during which the spores imbibe water and establish sensitivity to irradiation, involves an oxidative process which can be r...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.55.2.150

    authors: Towill LR,Ikuma H

    更新日期:1975-02-01 00:00:00

  • Mercury-induced Ethylene Formation and Abscission in Citrus and Coleus Explants.

    abstract::Mercury vapor induces ethylene formation and abscission in Citrus and Coleus explants. Both responses are markedly greater in the absence of CO(2). The stimulation of these metabolically complex processes indicates that the action of mercury vapor is not consistent with the more popular conception of mercury toxicity....

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.57.4.628

    authors: Goren R,Siegel SM

    更新日期:1976-04-01 00:00:00

  • Characterization of root hair cell walls as potential barriers to the infection of plants by rhizobia : the carbohydrate component.

    abstract::The sugar compositions of root hairs of a variety of plant species were determined. Root hairs of legumes had very similar compositions, whereas those of different families varied widely. Only approximately 50% of the weight of the root hairs of legumes could be accounted for by sugar. Up to 80% of the weight of root ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.86.2.638

    authors: Mort AJ,Grover PB

    更新日期:1988-02-01 00:00:00

  • Membrane Response to Diclofop Acid Is pH Dependent and Is Regulated by the Protonated Form of the Herbicide in Roots of Pea and Resistant and Susceptible Rigid Ryegrass.

    abstract::Electrophysiological studies in roots of pea (Pisum sativum L.) and rigid ryegrass (Lolium rigidum Gaud.) seedlings were conducted to elucidate the mechanism involved in the membrane response to the herbicide diclofop. In pea, a dicotyledonous plant insensitive to diclofop, membrane depolarization at varying pH values...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.102.4.1331

    authors: DiTomaso JM

    更新日期:1993-08-01 00:00:00

  • Ubiquitin-Proteasome Dependent Regulation of the GOLDEN2-LIKE 1 Transcription Factor in Response to Plastid Signals.

    abstract::Arabidopsis (Arabidopsis thaliana) GOLDEN2-LIKE (GLK) transcription factors promote chloroplast biogenesis by regulating the expression of photosynthesis-related genes. Arabidopsis GLK1 is also known to participate in retrograde signaling from chloroplasts to the nucleus. To elucidate the mechanism by which GLK1 is re...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.16.01546

    authors: Tokumaru M,Adachi F,Toda M,Ito-Inaba Y,Yazu F,Hirosawa Y,Sakakibara Y,Suiko M,Kakizaki T,Inaba T

    更新日期:2017-01-01 00:00:00

  • Transcriptome analysis of proliferating Arabidopsis endosperm reveals biological implications for the control of syncytial division, cytokinin signaling, and gene expression regulation.

    abstract::During the early stages of seed development, Arabidopsis (Arabidopsis thaliana) endosperm is syncytial and proliferates rapidly through repeated rounds of mitosis without cytokinesis. This stage of endosperm development is important in determining final seed size and is a model for studying aspects of cellular and mol...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.108.128108

    authors: Day RC,Herridge RP,Ambrose BA,Macknight RC

    更新日期:2008-12-01 00:00:00

  • Fructokinase (Fraction IV) of Pea Seeds.

    abstract::A fructokinase (EC 2.7.1.4) was obtained from pea (Pisum sativum L.) seeds. This enzyme, termed fructokinase (fraction IV), was specific for fructose as substrate and had little activity with glucose or mannose. Excess fructose inhibited the enzyme at the optimum pH (8.2) but not at pH 6.6. MgATP was inhibitory at pH ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.60.5.666

    authors: Turner JF,Harrison DD,Copeland L

    更新日期:1977-11-01 00:00:00

  • Evidence for circadian regulation of starch and sucrose synthesis in sugar beet leaves.

    abstract::Starch accumulation and sucrose synthesis and export were measured in leaves of sugar beet (Beta vulgaris L.) during a period of prolonged irradiance in which illumination was extended beyond the usual 14-hour day period. During much of the 14-hour day period, approximately 50% of the newly fixed carbon was distribute...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.99.4.1393

    authors: Li B,Geiger DR,Shieh WJ

    更新日期:1992-08-01 00:00:00

  • Metabolic Interactions between Spinach Leaf Nitrite Reductase and Ferredoxin-NADP Reductase: Competition for Reduced Ferredoxin.

    abstract::Steady state rates of NADP reduction decline upon commencement of nitrite reduction in reconstituted chloroplast preparations. Similarly, steady state rates of nitrite reduction are lower, but not zero, during concurrent NADP reduction. These results imply that competition for substrate occurs and suggest that nitrite...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.77.2.318

    authors: Baysdorfer C,Robinson JM

    更新日期:1985-02-01 00:00:00